In this work, the topology-optimized lattices structures were designed to serve as ductile nylon reinforcements in lattice-enhanced cementitious composites. The mechanical behavior of the prepared composites was examined via a flexural test where the strength and mid-span deflection were especially concerned. The digital image correlation (DIC) approach was employed to monitor and evaluate the failure pattern and strain distribution. Further investigation was conducted on the mechanical effects of two major optimization factors in the lattice design process, namely cell size and optimization volume fracture. Finally, the improved ductile performance of the suggested composites was compared to that of prior works. The results indicated that the proposed topology-optimized lattices significantly promote the mechanical performance of the prepared composites, i.e., strength and ductility. It may present a higher ductility enhancement efficiency than homogenous lattices under an identical volume. The cell size of lattices is inversely related to the enhancing effects, and more extensive optimization volume fracture enhances composite strength and ductility. Compared with previous works, this study gained advancement in terms of printing materials, unit topology, and cement types. The topology-optimized lattice generally provides a novel solution to the ductility enhancement effect, providing both superior performance and greater design flexibility.