Distribution Of Plasma Proteins Research Articles

Effect of ACTH on distribution of plasma proteins of patients with pulmonary tuberculosis.

Summary1. Albumin level and A/G ratio of pulmonary tuberculosis patients are reduced from normal value. 2. Levels of gamma globulin, fibrinogen, and alpha 1 globulin are considerably higher than those of normal individuals. 3. ACTH depresses the gamma globulin and fibrinogen, and raises the albumin values and A/G ratio toward normal levels. Gamma globulin is less susceptible to immediate changes than the latter two components. 4. Cessation or reduction of ACTH therapy brought about a reversion of protein fractions to values similar to those noted in control samples. 5. Values for alpha 1, alpha 2, and beta globulins appeared to be variable under the influence of ACTH.

Distribution of plasma proteins in the tissues of infants and children

Distribution of plasma proteins in the tissues of infants and children

Electrophoretic studies of plasma and urinaryproteins in children with lipoid nephrosis

Summary 1. Electrophoretic analyses have been made of plasma samples from eleven children with lipoid nephrosis. Distribution of the plasma protein fractions was determined in various stages of the disease and after intravenous administration of concentrated human serum albumin for periods of one to sixty days. 2. In general, the following changes from the normal percentage distribution of plasma proteins were noted in nephrosis: (a) a decrease in albumin, varying with the severity and duration of the disease; (b) an increase in α 2 - and β -globulins and fibrinogen; (c) often an increase in α 1 -globulin; (d) a decrease in γ -globulin. 3. Administration of concentrated serum albumin daily for long periods resulted in some cases in an increase in albumin, and usually a decrease in β -, α 2 - and sometimes α 1 -globulin concentrations. However, the changes were not maintained after albumin therapy was discontinued. 4. In nephrosis the urine may contain all of the protein fractions present in plasma except fibrinogen. Administration of concentrated human serum albumin intravenously results in an increase primarily in albumin excretion, although minor changes in the excretion of the globulin fractions may occur. Ascitic and pleural fluids from children with nephrosis have a slightly differentdistribution of protein fractions than plasma samples from the same children.

Study of the Effect of Bloodletting and Blood Transfusion on Plasma Protein Distribution

he author carried out experimental and clinical studies to clarify the issue of the effect on the distribution of plasma protein of completely opposite manipulations - bloodletting and blood transfusion.

Plasma Protein Distribution by the Modified Wu Colorimetric Method During Glucose Tolerance Tests.

Wu1 published a colorimetric method for the analysis of plasma proteins by use of a phospho-18-molybdictungstic acid (phenol) reagent. The method of fractionation and the standard used was modified by Gibson2 and his method in essential details has been followed in this work. Six cc. of blood were removed by vena puncture, oxalated and centrifuged to obtain the clear plasma. One cc. of the plasma thus obtained was placed in a 15 cc. centrifuge tube and 2 cc. of distilled water added. To this was added 1 cc. of saturated ammonium sulphate thus bringing the solution to one-fourth saturation, precipitating out the fibrinogen. After standing 10 minutes the fibrinogen was lightly centrifuged and the fluid decanted into a dry tube. One cc. of the fluid was placed into a second 15 cc. centrifuge tube with 1/2 cc. of water and 1 cc. of saturated ammonium sulphate. This brought the mixture to half saturation thus precipitating out the globulins. After standing 15-20 min. the tubes were centrifuged rapidly, and the...

Erythrocyte Sedimentation Rate of Citrated Blood and Distribution of Plasma Proteins.

Erythrocyte Sedimentation Rate of Citrated Blood and Distribution of Plasma Proteins.