Distribution Of Pesticides Research Articles

Geographical Distribution and Pattern of Pesticides in Danish Drinking Water 2002-2018: Reducing Data Complexity.

Pesticides are a large and heterogenous group of chemicals with a complex geographic distribution in the environment. The purpose of this study was to explore the geographic distribution of pesticides in Danish drinking water and identify potential patterns in the grouping of pesticides. Our data included 899,169 analyses of 167 pesticides and metabolites, of which 55 were identified above the detection limit. Pesticide patterns were defined by (1) pesticide groups based on chemical structure and pesticide–metabolite relations and (2) an exploratory factor analysis identifying underlying patterns of related pesticides within waterworks. The geographic distribution was evaluated by mapping the pesticide categories for groups and factor components, namely those detected, quantified, above quality standards, and not analysed. We identified five and seven factor components for the periods 2002–2011 and 2012–2018, respectively. In total, 16 pesticide groups were identified, of which six were representative in space and time with regards to the number of waterworks and analyses, namely benzothiazinone, benzonitriles, organophosphates, phenoxy herbicides, triazines, and triazinones. Pesticide mapping identified areas where multiple pesticides were detected, indicating areas with a higher pesticide burden. The results contribute to a better understanding of the pesticide pattern in Danish drinking water and may contribute to exposure assessments for future epidemiological studies.

Dynamics, Residue Distribution and Dietary Risk Assessment of Pesticides and Metabolites in Rose from Cultivation to Consumption

Dynamics, Residue Distribution and Dietary Risk Assessment of Pesticides and Metabolites in Rose from Cultivation to Consumption

Screening of pesticide distributions in foods of animal origin: a matrix-based approach for biotransfer factor modeling of grazing mammals.

Pesticides are detected in a wide array of foods of animal origin, posing a threat to food safety and human health. Here, to facilitate the management of pesticide residues in livestock products, we proposed a screening model for simulating pesticide biotransfer factors (BTFs, ratio of steady-state pesticide concentration between animal tissues and feed) in mammalian bodies. The proposed model was developed based on simple matrix algebra using first-order kinetics. Simulation of over 700 pesticides in common food products derived from cattle and sheep indicated that pesticide biotransfer is a balancing process between uptake efficiency from the gut lumen and the uptake-elimination ratio (ratio of the overall uptake and elimination rate constants) in individual tissues. Furthermore, we parameterized the developed BTF model using the octanol-water partition coefficient. The simulated pesticide BTFs could be categorized into three lipophilicity phases, namely, the lipophilicity-boosting (log KOW < 4), lipophilicity-balancing (4 ≤ log KOW ≤ 8), and lipophilicity-limiting (log KOW > 8) phases, and the simulated general trends of BTFs were consistent with the experimental data. Although the metabolic rate constants of pesticides in mammalian bodies warrant further evaluation, the model proposed here can assist in the risk assessment and regulatory management of pesticide residues in foods of animal origin.

Distribution and Potential Sources of OCPs and PAHs in Waters from the Danshui River Basin in Yichang, China.

To investigate the concentrations, spatial distribution, potential sources and mass fluxes of organochlorine pesticides (OCPs) and polycyclic aromatic hydrocarbons (PAHs) in waters from the Danshui River Basin, a total of 20 water samples were collected and analyzed from a karstic river in Western Hubei of Central China. The average concentrations of total OCPs and PAHs in the river water were 4719 pg·L−1 and 26.2 ng·L−1, respectively. The characteristic ratios of different isomers and the composition analysis of individual OCPs and PAHs revealed that HCHs originated from a mixed input of technical HCHs and Lindane, DDTs were mainly from technical DDTs, and PAHs mainly originated from biomass and coal combustion. The mass flux analysis showed that PAHs had a higher emission and heavier burden than OCPs in the Danshui River Basin. OCPs and PAHs emitted from agricultural or other human activities could enter the groundwater and then be transported to the surface/river water in the karst area. The adsorption of OCPs and PAHs by particles and the sedimentation of particles could be the primary processes to intercept these pollutants in the water of the karstic river system.

Level, source, and distribution of organochlorine pesticides (OCPs) in agricultural soils of Tanzania.

This study investigated the level, composition, and spatial and vertical distribution of the organochlorine pesticides (OCPs) at 0-2cm and 2-20cm in the agricultural surface soils from Southeastern to Central-western Tanzania. Although the most abundant OCPs were DDT with a mean concentration of 2.29ng/g, dieldrin (1.57ng/g), and methoxychlor (0.79ng/g), HCH was the most dominant (with detection frequency of 88%). OCP dominance was in the Southern Highlands, which is the most productive agricultural zone. Though there were indicators of recent inputs for some sites, OCP contamination was mainly historical. DDT contamination was dominated by p,p'-DDE and resulted from both technical DDT and dicofol while HCH contamination was dominated by γ-HCH and resulted from both technical HCH and lindane. Based on depth, the OCPs dominated mainly the upper 2cm, which was associated with soil and environmental factors rather than recent inputs since most of the detected compounds were historical. Nevertheless, some sites showed exceptional high abundance in the lower soil with more concentration of parent compounds. Therefore, this study recommends the need for further studies on the influence of soil properties on OCPs' transport in the soil, surface water, and air. Besides, detection of recent inputs at some sites calls for more mapping of the OCPs in the country to strengthen their control and prevention of future risks.

Evaluation and risk assessment of organochlorine pesticide residues in some fish species in south-south Nigeria

This study reports the presence, distribution pattern, concentration levels and health risk assessment of organochlorine pesticide residues in fresh and cured fish species in South-South Nigeria. The concentrations of organochlorine pesticides in fresh and cured fish were analysed with Gas Chromatography/ Mass Spectrophotometry (GC-MS) after Soxhlet extraction of the fish species. The study revealed that no organochlorine pesticide was present in fresh fish. However, cured fish species from all the markets sampled had at least two organochlorine pesticide residues which ranged from endosulfan (0.252±0.0022–748±0.004ug/g), lindane (0.386±0.010–0.443±0.009 ug/g), heptachlor-epoxide (0.328±0.005–3.162±0.010ug/g), ortho-para-dichloro-diphenyl-trichloro-ethane (o,p’-DDT) (0.021±0.001–0.180±0.030ug/g) and aldrin (0.140±0.040–2.770±0.020ug/g). These levels are far higher than the maximum residual limit and allowable daily intake values recommended by World Health Organization (WHO) and Food and Agricultural Organization (FAO). There were no pesticide residues in fresh fish probably because the fisher folks in the study area may have abolished the use of obnoxious fishing methods due to the awareness of threats to the aquatic ecosystem. The high percentage occurrences, amounts and distribution of organochlorine pesticides in cured-fish from markets sampled could be as a result of the fact that these organochlorine pesticides were consistently used in the study area by fish processors, distributors and sellers. Human risk assessment of this study revealed a potential danger in the consumption of cured-fish containing the organochlorine pesticides seen in the study.

DDT in Crimea coastal waters and blubber of Black Sea cetaceans

This research is based on the multi-year data on the distribution of organochlorine pesticides of the DDT group in the water of the coastal Black Sea areas of Crimea, obtained in expeditions of the “Professor Vodyanitsky” research vessel from 1999 to 2020. Study of content of DDT and its metabolites (DDTs) in the blubber of three species of Black Sea cetaceans that were astrand on the Black Sea coast from the 1980s to the 2020s was grounded on own and literature data. While there was an overall significant variability in ∑DDT content during the study period, there was a significant downward trend in their concentrations in the water of coastal areas of Crimea. Despite the significantly decreased DDTs pollution of the habitat of the Black Sea marine mammals over the last 10 years, high concentrations of DDTs were accumulated in their blubber, the maximum value of the sum of DDTs concentrations reached (163 µg/g of lipids) in the blubber of adult depleted bottlenose dolphin. The minimum concentrations were determined in the blubber of a newborn harbour porpoise female. In blubber samples up to 70% was of DDE, DDD - from 21 to 24%, and the concentration of the initial DDT varied from 6 to 14%. Based on the analysis of own and literature data, it is assumed that in the modern period, as compared to the 1990s, there is a downward trend in the content of the DDTs in the blubber of Black Sea cetaceans.

Spatiotemporal distribution and risk assessment of organophosphorus pesticides in surface water and groundwater on the North China Plain, China

90 groundwater samples and 14 surface water samples were collected in wet season (summer) and dry season (winter) in the North China Plain (NCP), and analyzed for 11 organophosphorus pesticides (OPPs). The results showed that the main types of OPPs in surface water and groundwater were dimethoate, dichlorvos, methyl-parathion, malathion in both summer and winter. The OPP concentrations in groundwater and surface water were higher in summer than in winter. In the vertical direction, the distribution characteristics of different four types of groundwater sampling points are different. In the horizontal direction: farmland adjacent to a river (FAR) > central farmland (CF) > nonfarm area adjacent to a river (NFAR) > central nonfarm area (CNF). The OPPs concentrations in surface water adjacent to farmland were higher than that in surface water adjacent to nonfarm area. The main factors influencing the distribution of OPPs in the groundwater and surface water were the interaction process between them, the groundwater flow field and the OPPs used in agricultural activities. The ecological risk of OPPs to surface water was greater in summer than in winter. Water Flea was at medium risk, and malathion had the greatest influence on Water Flea in both summer and winter. The non-carcinogenic and carcinogenic risks of the four main OPPs in surface water were higher than in groundwater, and were higher in summer than in winter, but they would not lead to adverse health effects on local residents.

Distribution of Pesticides in Different Commonly Grown Vegetables of Cameron Highlands, Pahang, Malaysia

Gas chromatography-electron capture detector (GC-ECD) is used to extract and analyse pesticides in vegetable samples collected from Cameron Highlands, Pahang, Malaysia. The limit of detection (LOD) for all pesticides was in the range of 0.03 to 4.5 ng g-1. Recoveries in cabbage, lettuce, and celery ranged from 61.8%-121%, 60-128% and 60%-114%, respectively. The relative standard deviation (RSD) ranged 0.2-15% in cabbage, 0.5-18% in lettuce and 3-19.8% in celery. Organochlorine pesticides (OCPs) concentrations increased down the valley with dichlorodiphenyldichloroethylene (DDE) having the highest concentration at 233 µg kg-1. Organophosphorus pesticides (OPPs) were found to be dispersed throughout the valley, with the highest concentration of parathion ethyl (133 µg kg-1) whereas the pyrethroid pesticides (PYRs) concentrations were comparatively less. The detection frequency in the wet season was highest (5 &lt; - &lt; 100 µg kg-1) for most pesticides. However, in the dry season the pesticides concentrations were higher, at &lt; 5 µg kg-1. PCA analysis indicated that farmers were using a mixture of pesticides.

Evaluation of the impact of effective management practices in mitigating the toxicity symptoms of pesticides preparation and storage in Kebbi State, Nigeria

The study Evaluated the Impact of Effective Management Practices in Mitigating the Toxicity Symptoms of Pesticides Preparation, Storage and Application in Kebbi State, Nigeria. Structured Questionnaires were used to obtained information from the respondents. Descriptive statistics comprising frequency distribution tables and percentages and were used for Data Analysis. Likert scale was used to determine farmer’s perception on the toxicity symptoms of pesticides of pesticides preparation, storage and application employed by farmers when handling pesticides. The result of the study showed that majority of the respondents (90%) were male while female formed the minority in pesticide usage with only (10%). The study also showed that all the respondents (100%) were married and engaged in agricultural activities. The study further revealed that all the respondents never had any formal training on the use of pesticide from either governmental or non -governmental organizations. The research study also found out that all (100%) of the respondents were not aware of the toxicity of pesticides during preparation, storage and application. Likert Scale Analysis showed a negative perception by farmers on awareness of pesticide toxicity during preparation, storage and application. On perception of the respondents on the toxicity of pesticides during preparation, storage and application, the study revealed that Nausea, Dizziness, Diarrhea, Respiratory Difficulty, Skin Irritation, Rashes, Fever, Peeling of the Skin, Vomiting, and Headache were the serious toxicity effects of pesticide. It is concluded that respondents had negative perception on pesticide management of toxicity of pesticides during preparation, storage and application. It is therefore recommended that appropriate authorities should enforce the use of protective clothing, appropriate equipment and correct handling practices when using pesticides. Existing pesticide regulations and monitoring policies should be enforced. Government should also intensify efforts at registering and controlling distribution of pesticides and banning hazardous ones. It should also enforce the making of less toxic pesticides available to farmers.

Structure and Evolution of the International Pesticide Trade Networks

To meet the increasing demand for food around the world, pesticides are widely used and will continue to be widely used in agricultural production to reduce yield losses and maintain product quality. International pesticide trade serves to reallocate the distribution of pesticides around the world. We investigate the statistical properties of the international trade networks of five categories of pesticides from the view angle of temporal directed and weighted networks. We observed an overall increasing trend in network size, network density, average in- and out-degrees, average in- and out-strengths, temporal similarity, and link reciprocity, indicating that the rising globalization of pesticides trade is driving the networks denser. However, the distributions of link weights remain unchanged along time for the five categories of pesticides. In addition, all the networks are disassortatively mixed because large importers or exporters are more likely to trade with small exporters or importers. We also observed positive correlations between in-degree and out-degree, in-strength and out-strength, link reciprocity and in-degree, out-degree, in-strength, and out-strength, while node’s local clustering coefficient is negatively related to in-degree, out-degree, in-strength, and out-strength. We show that some structural and dynamic properties of the international pesticide trade networks are different from those of the international trade networks, highlighting the presence of idiosyncratic features of different goods and products in the international trade.

Distribution of pesticides and some of their transformation products in a small lentic waterbody: Fish, water, and sediment contamination in an agricultural watershed

More than 20 years after the Water Framework Directive was adopted, there are still major gaps in the sanitary status of small rivers and waterbodies at the head of basins. These small streams supply water to a large number of wetlands that support a rich biodiversity. Many of these waterbodies are fishponds whose production is destined for human consumption or for the restocking of other aquatic environments. However, these ecosystems are exposed to contaminants, including pesticides and their transformation products. This work aims to provide information on the distribution, diversity, and concentrations of agricultural contaminants in abiotic and biotic compartments from a fishpond located at the head of watersheds. A total of 20 pesticides and 20 transformation products were analyzed by HPLC-ESI-MS/MS in water and sediment sampled monthly throughout a fish production cycle, and in three fish species at the beginning and end of the cycle.The highest mean concentrations were found for metazachlor-OXA (519.48 ± 56.52 ng.L−1) in water and benzamide (4.23 ± 0.17 ng g−1 dry wt.) in sediment. Up to 20 contaminants were detected per water sample and 26 per sediment sample. The transformation products of atrazine (banned in Europe since 2003 but still widely used in other parts of the world), flufenacet, imidacloprid (banned in France since 2018), metazachlor, and metolachlor were more concentrated than their parent compounds. Fewer contaminants were detected in fish and principally prosulfocarb accumulated in organisms during the cycle.Our work brings innovative data on the contamination of small waterbodies located at the head of a basin. The transformation products with the highest frequency of occurrence and concentrations should be prioritized for further environmental monitoring studies, and specific toxicity thresholds should be defined. Few contaminants were found in fish, but the results challenge the widely use of prosulfocarb.

Organochlorine pesticides and chlorpyrifos in the sea anemone Bunodosoma zamponii (Actiniaria: Actiniidae) from Argentina's southeastern coast

Temporal and spatial distribution of organochlorine pesticides (OCPs) and the organophosphate pesticide chlorpyrifos, one of the main insecticides used in Argentina, was evaluated in two populations of the sea anemone Bunodosoma zamponii living under different anthropological stressors: Las Delicias (LD) adjacent to a wastewater plant, and Punta Cantera (PC) a reference site. Pesticides were analyzed throughout the year in water, sediments and whole organisms. Chlorpyrifos represented 50% of the total pesticide found in water samples during winter. HCHs and drins were predominant in sediment samples, mainly in LD. Total pesticide concentration in anemones from LD was higher than those from PC during winter (mainly associated with HCHs, endosulfans, DDTs and chlorpyrifos levels), coincident with the main period of effluent discharge to the coast after pesticide applications and also the rainiest season. Dissimilarities among anemones populations could stem from a differential input of pesticides in each site and/or a contrasting physiological status of the populations.

Modeling Distribution and Dissipation Kinetics of Pesticides in Peel and Medulla Tissues of Postharvest Tuber Crops

Modeling Distribution and Dissipation Kinetics of Pesticides in Peel and Medulla Tissues of Postharvest Tuber Crops

CFD AND TEST OF SINGLE SIDE GANTRY BOOM SPRAYER FOR APPLE ORCHARDS

Aiming at addressing the uneven pesticide distribution when spraying high-spindle apple trees, the parameters of a homemade profiling boom sprayer were analysed, and the optimal parameter combinations of the profiling sprayer were determined. Fluent was used to simulate the spray for various parameter values. The goodness of fit (R2) with experimentally measured data was 0.9270, which verified the accuracy of the numerical simulations. The response surface methodology (RSM) method was used to obtain optimal parameter values from the simulations, and the effective collection rate and spray distribution coefficient of variation were introduced as response values. A sprayer prototype was manufactured based on the optimal parameters determined by RSM analysis, and the results were verified by vertical droplet distribution tests. The results showed that the spray quality increased by 0.7%, 0.9%, and 0.3% for the three best parameters combinations, the droplet distribution was more uniform, and the effect of the profiling spray was better than that of the control tests. The results can provide guidance for the design of sprayers for orchards, and the optimal combination of parameters can provide a basis for subsequent research on precision modelling of profile spraying.

Vertical distribution and effect of historical residual organochlorine pesticides on microbial community structure in sediment cores from an abandoned oxidation pond after dredging for 15 years.

The vertical distribution pattern of 19 organochlorine pesticides (OCPs), together with microbial ester-linked fatty acid methyl ester (EL-FAME) profiles were investigated in sediments from an abandoned oxidation pond of Ya-Er lake, China, which had been heavily polluted by hexachlorocyclohexanes (HCHs) and chlorobenzenes in 1980s. Subsurface sediment samples were taken from five sediment cores along the transect running from the lakeshore (0.5~2.7 m in depth) to lakebed (0.1~0.4 m). The total OCP concentration ranged from 29.8 to 941.8 ng g-1 dw. Hexachlorobenzene (HCB), HCHs, and dichlorodiphenyl-trichloroethanes (DDTs) were the three dominant OCP classes, accounting for 26.5-97.4%, 1.8-33.2%, and 0.4-15.5% of the total OCP concentration, respectively. Hot spots of HCB, HCHs, and DDTs were detected at 0.9~2.7 m deep layers of the lakeshore, where was oncethe maindredged sediment backfill site for in-situ remediation of the oxidation pond in 2002-2004. HCHs and HCBstill showed high potential ecological risks. The sources of OCPs were identified and quantified using principal component analysis with absolute principal component scores-multiple linear regression model. The firstthree major sources were persistent residues, recent agricultural input, and historical industrial input, contributing on average 28.2%, 17.9%, and 17.1% of total OCPs, respectively. Redundancy analysis of microbial EL-FAME profiles and nine dominant OCPs revealed that the spatial variation in microbial community structure was significantly corresponded with the OCP composition. This is the first study highlighting the concern on historical industrial inputs of OCPs in subsurface sediments of the lakeshore disposal zone. The findings could help to distinguish the artificial backfill sediments from undisturbed polluted sediments for optimization of further dredging plans.

Removal and fate of pesticides in a farm constructed wetland for agricultural drainage water treatment under Mediterranean conditions (Italy)

A non-waterproofed surface flow constructed wetland (SFCW), treating agricultural drainage water in Northern Italy, was investigated to gain information on the potential ability for effective pesticide abatement. A mixture of insecticide imidacloprid, fungicide dimethomorph, and herbicide glyphosate was applied, by simulating a single rain event, into 470-m-long water course of the SFCW meanders. The pesticides were monitored in the wetland water and soil for about 2 months after treatment. Even though the distribution of pesticides in the wetland was not uniform, for each of them, a mean dissipation of 50% of the applied amount was already observed at ≤7 days. The dissipation trend in the water phase of the wetland fitted (r2 ≥ 0.8166) the first-order model with calculated DT50 of 20.6, 12.0, 5.8, and 36.7 days for imidacloprid, dimethomorph, glyphosate, and the glyphosate metabolite AMPA, respectively. The pesticide behavior was interpreted based on the chemical and physical characteristics of both the substances and the water-soil system. Despite the fast abatement of glyphosate, traces were detected in the water until the end of the trial. The formation of soluble 1:1 complex between glyphosate and calcium, the most representative cation in the wetland water, was highlighted by infrared analyses. Such a soluble complex was supposed to keep traces of the herbicide in solution.

Examination of the economic and environmental factors affecting pesticides handling and usage and its effects on man and the environment in Danko/Wasagu Local Government Area, Kebbi State, Nigeria

The study examined the Economic and Environmental Factors Influencing Pesticides Handling and Usage in Danko/Wasagu Local Government area of Kebbi State, Nigeria. Structured Questionnaires were used to obtained information from the respondents. Descriptive statistics comprising frequency distribution tables and percentages, Mean and Ranking were used for Data Analysis. The result of the study showed that majority of the respondents (90%) were male while female formed the minority in pesticide usage with only (10%). The study also showed that all the respondents (100%) were married and engaged in agricultural activities. The study further revealed that all the respondents never had any formal training on the use of pesticide from either governmental or non -governmental organizations. The research study also found out that all (100%) of the respondents were not aware of pesticide effects on man and environment. On the economic and environmental factors affecting pesticide handling and usage, it was found out that, the effect of pesticide on man and the environment is in the form of Nausea, Dizziness, Diarrhea, Respiratory Difficulty, Skin Irritation, Rashes, and Fever, Peeling of the Skin, Vomiting, and Headache. These were discovered to be the serious effects of pesticides on man. However, it was found out that the effects of pesticides on the environment were in the form of pollution, annihilation of the micro fauna and flora and reduction in land viability on the occasion of repeated usage over time, pollution of water bodies, pollution of the atmosphere, depletion of plat population etc. It is concluded that respondents were posed with serious challenges on pesticide usage and handling. It is therefore recommended that appropriate authorities should enforce the use of protective clothing, appropriate equipment and correct handling practices when using pesticides. Existing pesticide regulations and monitoring policies should be enforced. Government should also intensify efforts at registering and controlling distribution of pesticides and banning hazardous ones. It should also enforce the making of less toxic pesticides available to farmers.

Drone Payload and Flying Speed Effects on Rotor Blades' RPM and Traveling Pattern for Agricultural Chemical Spraying

The sprayed chemicals by drones have been widely reported to be off-targeted and not uniformly distributed. This study aims to evaluate the drone blade’s revolutions per minute (RPM) and its travelling pattern at different payloads and flight speeds. The obtained results were used to relate to the potential effects on the quantity and quality of spraying. In a test flight on an area of 1000 m2, a hexacopter, Advansia A1 was tested in 6 different flying paths of 56 m length. The drone was set to fly at 5 payloads (10, 8, 6, 4, and 2 kg) and 4 flying speeds (i.e. 1, 3, 5, and 7 m.s-1) combinations. The drone travelling pattern and individual rotor blade rpm at each payload-flying speed combinations were analysed. From the result, the RPM of each rotor blade were found to decrease by 14 to 20% as the payload was decreased from 10kg to 0kg. Thus, in actual spraying activities, the changes in RPM could produce a downwash airflow pattern that continually varies from starting point up to the finishing point that would effect on pesticide's distribution along the flying path. On drone travelling pattern, at higher flying speed, a much lesser time and distance was required for the drone to be stabilized to the targeted speed. This relates to the longer time needed by the drone to accelerate and decelerate. The average real speed of the drone was notably reduced to 0.96, 2.72, 3.83 and 4.05 m.s-1, in which, it was, far less than the initial specified speed set at 1, 3, 5, and 7 m.s-1, respectively. The drone flying pattern during spraying needs to be considered for application rate determination to avoid for the crops to be under or over pesticide applications. The obtained finding is remarkably critical and useful in ensuring the efficiency of agricultural chemical spraying activities using drone.

High-throughput screening and spatial profiling of low-mass pesticides using a novel Ti3C2 MXene nanowire (TMN) as MALDI MS matrix

Pesticides play critical roles in agricultural fields; however, pesticide residues can cause serious damage to human health and the ecological environment; therefore, developing a rapid and sensitive method for pesticide detection is urgently needed. Nanostructure-assisted matrix laser desorption/ionization (MALDI) mass spectrometry (MS) has great potential for the detection of low-mass pesticides. In this study, a novel Ti3C2 MXene nanowire (TMN) was prepared by a facile sol-gel method and served as a matrix to enhance MALDI MS performance in the analysis of pesticides in positive ion mode. The TMN showed superior performance in the high-throughput detection of six kinds of pesticides (organophosphorus, organochlorine, carbamate, neonicotinoids, triazole, and oxadiazines), with ultrahigh sensitivity (detection limits at sub-ppt levels), remarkable repeatability, excellent salt tolerance, and extremely low background compared to traditional organic matrices due to the specific polyaromatic structure and the doping of nitrogen. Furthermore, this matrix was successfully employed for the analysis of residual pesticides in traditional Chinese herbs, and the level of diniconazole was quantified with a linear range of 0–50 ng/mL (R2 > 0.99). More importantly, the spatial distribution of various endogenous compounds (e.g., amino acids and saccharides, fatty acids, alkaloids, and plant hormones) and xenobiotic pesticides from the intact root of the medicinal plant P. quinquefolium was clearly visualized using the TMN self-assembly film as a matrix for MALDI imaging mass spectrometry (IMS). With superior advantages such as sensitivity, simplicity, rapidness, and minimal sample requirement, TMN as a matrix-assisted MALDI MS shows great promise for various applications.