With the developing process from the spermatid to the sperm, the great changes of membrane structure are token place both in plasma membrane and nuclear membrane. This note will report the development of polarization in membrane and the distribution of nuclear pores and protein particles during spermiogenesis, and also compare the number of protein in P-face with that in E-face.Mice, Rats, Rabbits and Dogs were operated by taking out the seminiferous tube. It was fixed in 2.5% glutaradehyde for 2 hrs 5 washed in cacodylated buffer, and then the sample was put in to 30% glycerol solution for 6-12hrs. Samples were put into liquid nitrogen rapidly and afterwards moved to BALZAR BAF-400D instrument to get Freeze-Fracture replica and double replica for observation with TEM.The expression and the development of the polarization in membrane structure of the spermatid. The spermatid in different stepshas different characteristics and there is more polarization for membrane structure following spermiogenesis.