Mass Balance Distribution Research Articles

Pharmacokinetics, mass balance, tissue distribution, metabolism, and excretion of [14C]aficamten following single oral dose administration to rats

The pharmacokinetics, metabolism, excretion, mass balance, and tissue distribution of [14C]aficamten were evaluated following oral administration of an 8 mg/kg dose in Sprague Dawley rats and in a quantitative whole-body autoradiography study in Long Evans rats. [14C]Aficamten accounted for ∼80% and a hydroxylated metabolite (M1) accounted for ∼12% of total radioactivity in plasma over 48-h (AUC0–48). Plasma t max was 4-h and the t 1/2 of total plasma radioactivity was 5.8-h. Tissues showing highest Cmax exposures were myocardium and semitendinosus muscle. Most [14C]aficamten-derived radioactivity was excreted within 48-h post-administration. Mean cumulative recovery in urine and faeces over 168-h was 8.3% and 90.7%, respectively. In urine and bile, unchanged aficamten was detected at <0.1 and <0.2% of dose, respectively; however, based on total radioactivity excreted in urine (8.0%) and bile (51.7%), approximately 60% of dose was absorbed. [14C]Aficamten was metabolised by hydroxylation with subsequent glucuronidation where the most abundant metabolite recovered in bile was M5 (35.2%), the oxygen-linked glucuronide of hydroxylated aficamten (M1a). The major metabolite detected in faeces was a 1,2,4-oxadiazole moiety ring-cleaved metabolite (M18, 35.3%), shown to be formed from the metabolism of M5 in incubations with rat intestinal contents solution.

A novel framework to investigate wind-driven snow redistribution over an Alpine glacier: combination of high-resolution terrestrial laser scans and large-eddy simulations

Abstract. Wind-driven snow redistribution affects the glacier mass balance by eroding or depositing mass from or to different parts of the glacier’s surface. High-resolution observations are used to test the ability of large-eddy simulations as a tool for distributed mass balance modeling. We present a case study of observed and simulated snow redistribution over Hintereisferner glacier (Ötztal Alps, Austria) between 6 and 9 February 2021. Observations consist of three high-resolution digital elevation models (Δx=1 m) derived from terrestrial laser scans taken shortly before, directly after, and 15 h after snowfall. The scans are complemented by datasets from three on-site weather stations. After the snowfall event, we observed a snowpack decrease of 0.08 m on average over the glacier. The decrease in the snow depth can be attributed to post-snowfall compaction and the wind-driven redistribution of snow. Simulations were performed with the Weather Research and Forecasting (WRF) model at Δx=48 m with a newly implemented snow drift module. The spatial patterns of the simulated snow redistribution agree well with the observed generalized patterns. Snow redistribution contributed −0.026 m to the surface elevation decrease over the glacier surface on 8 February, resulting in a mass loss of −3.9 kg m−2, which is on the same order of magnitude as the observations. With the single case study we cannot yet extrapolate the impact of post-snowfall events on the seasonal glacier mass balance, but the study shows that the snow drift module in WRF is a powerful tool to improve knowledge on wind-driven snow redistribution patterns over glaciers.

Biochar mitigates the adverse effects of antimony on methanogenic activity: role as methane production-enhancer.

Antimony, extensively used in energy applications, poses toxicity and contamination concerns, especially in anaerobic environments where its impact on microbial activity is poorly understood. Emerging remedies, like biochar, show promise in soil and water treatment. This study investigates biochar's influence on methanogenic activity under Sb(V) and Sb(III) stress using anaerobic sludge as inoculum and lactate as the carbon source. Sb(III) and Sb(V) were introduced at varied concentrations (5-80 mg/L), with or without biochar, monitoring changes in biogas production, pH, Sb, and lactate levels over time. Experiments with Sb(V) also involved calculating mass balance and electron distribution. Results showcased the following significant enhancements: biochar notably improved COD removal and biogas production in Sb(III) spiked conditions, up to 5-fold and 2-fold increases, respectively. Sb(III) removal reached up to 99% with biochar, while in high Sb(V) concentrations, biochar reduced the adverse effect on biogas production by 96%. Adsorption capacities favored biomass (60.96 mg Sb(III)/gVSS, and 22.4 mg Sb(V)/gVSS) over biochar (3.33 mg Sb(III)/g, and 1.61 mg Sb(V)/g) for both Sb species. This study underscores biochar's potential to mitigate metalloid impact on methanogenic activity while aiding Sb removal from liquid phase, suggesting promising implications for remediation and methane production enhancement strategies.

Thinning and surface mass balance patterns of two neighbouring debris-covered glaciers in the southeastern Tibetan Plateau

Abstract. Debris-covered glaciers are a common feature of the mountain cryosphere in the southeastern Tibetan Plateau. A better understanding of these glaciers is necessary to reduce the uncertainties in regional water resource variability and to anticipate potential cryospheric risks. In this study, we quantified the seasonal thinning and surface mass balance patterns of two neighbouring debris-covered glaciers (23K Glacier and 24K Glacier) in the southeastern Tibetan Plateau with four unpiloted aerial vehicle surveys and in situ measurements. We observed that the thinning of 23K Glacier was ∼2–7 times greater than that of the 24K Glacier for annual and cold periods. The surface velocity of the 24K Glacier is higher than that of the 23K Glacier (∼5–6 times) for all periods. In contrast with the thinning patterns, the surface mass balance patterns of the two glaciers closely agree across the different periods. We found that the surface mass balance distribution strongly correlated with the spatial distribution of debris thickness for both glaciers. Ice cliffs and supraglacial ponds are prevalent on the surface of these glaciers (∼4.4–7.2±0.5 %) and enhance melt overall (enhancement factor: ∼2.5) but do not control the surface mass balance pattern of either glacier. This comparison study of two neighbouring glaciers confirms the significance of both glacier dynamics and debris thickness in controlling thinning and melt for the different debris-covered glaciers of the southeastern Tibetan Plateau in the context of climate change.

Glaciers Variation at ‘Shocking’ Pace in the Northeastern Margin of Tibetan Plateau from 1957 to 21st Century: A Case Study of Qiyi Glacier

Accelerating glacier shrinkage is one of the most consequential of global warming. Yet, projections for the region remain ambiguous because of the tremendous spatial heterogeneity, especially in the Qilian Mountains, where glacier melt runoff is a vital water resource for the arid downstream area. To better understand glacier changes in this region, this study took regional representative Qiyi Glacier as an example and applied an enhanced distributed surface mass balance (SMB) model to glimpse the SMB variation and possible impacts on melt runoff under the RCP 4.5 and RCP 8.5 scenarios. Further, we combined a modified volume-scaling method to update the glacier geometry gradually to enhance long-term reliability. When forced with observed daily temperature and precipitation, the reconstructed glacier SMB, from 1957 through 2013, agrees well with the in situ observations. The result indicates an abrupt change for SMB from positive to negative in 1992 and subsequent mass accelerated loss after 2000. The increased summer air temperature and the pattern of large-scale atmospheric circulation shifts might both cause these changes. Using projected climate forcing from as many as 31 coupled GCMs from the CMIP 5 ensemble, the Qiyi Glacier is projected to undergo sustained SMB loss throughout the 21st century for both RCPs. By 2100, the Qiyi Glacier will lose ~25 m water equivalent (w.e.) for RCP 4.5 and ~37 m w.e. for RCP 8.5. Whereas the glacier area will shrink by 43% for RCP 4.5 and 54% for RCP 8.5 relative to 2013 glacier content, corresponding to the volume of the Qiyi Glacier will lose by 54% for RCP 4.5 and by 65% for RCP 8.5, accordingly. Simultaneously, the glacier terminus will experience extreme melts. The terminus elevation of the Qiyi Glacier will retreat from 4310 m a.s.l. in 2013 to 4810 m a.s.l. (RCP 4.5) and 4838 m a.s.l. (RCP 8.5) by the end of 2100, which will exceed the multi-year average ELA (4749 m) from 1957 to 2013. If the warming trends keep and glaciers melt like the Qiyi Glacier with this ‘shocking’ rate, it will raise the possibility of crippling, long-term water shortages for Hexi corridors.

Zinc and cadmium contamination in an Avicennia schaueriana mangrove environment: Mass balance and tissue distribution.

The leachate pond of a Zn processing plant in Rio de Janeiro, Brazil, released Zn and Cd into a mangrove during three decades. Soil, root, stems, leaves and phloem bark samples of Avicenna schaueriana from the mangrove were collected and analyzed. Zn and Cd levels were measured by atomic absorption and were much more concentrated in soil at 76kg/ha and 424kg/ha respectively than in the trees (272.3kg/ha Zn and 0.046kg/ha Cd). This species shows a protective mechanism against uptake. The high content of Cd in the bark phloem was due to exposure to tidal flooding with high concentrations of Cd, not translocation from the roots. Allometry, and the biomass of roots, stems, leaves and bark showed that only a small percentage was phytoextracted by A. schaueriana, so this species under the described concentrations cannot phytoaccumulate and should not be used in phytoextraction.

Composite Structured M/Ce0.75Zr0.25O2/Al2O3/FeCrAl (M = Pt, Rh, and Ru) Catalysts for Propane and n-Butane Reforming to Syngas.

Here, we report the preparation, characterization, and performance of reforming propane and n-butane into a syngas of composite structured M/Ce0.75Zr0.25O2/Al2O3/FeCrAl (M = 0.46 wt.% Pt, 0.24 wt.% Rh, and 0.24 wt.% Ru) catalysts. The catalysts are composed of a high-heat-conducting FeCrAl block with preset geometry, with a surface nearly totally covered by θ-Al2O3. Afterwards, a layer of ceria–zirconia mixed oxide was deposited. The formed oxide coating was used as a support for 2–3 nm sized Pt, Rh, or Ru nanoparticles. The performance of the catalysts in propane steam reforming decreased in the order of Rh ≈ Ru > Pt. The reformates obtained in the propane steam reforming over Rh- and Ru/Ce0.75Zr0.25O2/Al2O3/FeCrAl at 600 °C and GHSV = 8300 h−1 contained 65.2 and 62.4 vol.% of H2, respectively, and can be used as a fuel for solid oxide fuel cells. In the oxidative steam reforming of propane at 700 °C and GHSV= 17,000 h−1, the activities of the Rh- and Pt-based catalysts were similar and the compositions of the outlet gas mixtures were quite close to equilibrium in both cases. Increasing the reagent flow rate to 25,600 h−1 showed stability of the Rh/Ce0.75Zr0.25O2/Al2O3/FeCrAl performance, whereas the Pt/Ce0.75Zr0.25O2/Al2O3/FeCrAl activity decreased. A mathematical model considering the velocity field, mass balance, pressure, and temperature distribution, as well as the reaction kinetics, was suggested for the propane steam and oxidative steam reforming over the Pt- and Rh/Ce0.75Zr0.25O2/Al2O3/FeCrAl catalysts. The model well described the experimental results.

Transgenerational mass balance and tissue distribution of PCBs and PCDD/Fs from grass silage and soil into cow-calf continuum

Grass-based suckling beef-derived foods occasionally exceed regulatory levels for polychlorinated biphenyls (PCBs) and dibenzo-p-dioxins/dibenzofurans (PCDD/Fs). Ensuring chemical safety requires understanding the cow-calf transgenerational PCB and PCDD/F fate. The current focus was on dairy cows, omitting transgenerational fate and suckling beef-related physiological effects. This study aimed to investigate PCB and PCDD/F absorption, distribution, metabolism, and excretion within 12 Simmental cows (six primiparous/six multiparous) and 12 calves fed with the milk of their respective mothers for 109 days prepartum until 288 days in milk (DIM), i.e., slaughter time. Eight cows were exposed to a grass silage-soil mixture. Four were decontaminated after DIM164 by receiving uncontaminated grass silage, which four control cows received. An input-output balance during gestation and lactation was computed from PCB, PCDD/F, and lipid inputs (solid feed/milk intakes), outputs (fecal/milk excretions), and body storage (initial/final burdens). At slaughter, PCB and PCDD/F tissue distribution, and lipid allometry were linked. Apparent PCB and PCDD/F absorption rates and metabolized fractions decreased with increasing chlorination. In calves, PCB absorption showed no effect due to chlorination (steady range: 71–87%). High-chlorinated PCB and PCDD/F absorption rates decreased when provided through soil. Cows excreted PCBs and PCDD/Fs via feces (50% relative to input) and milk (9%) and accumulated only 5% in their body, whereas calves accumulated the largest fraction of the total input in their bodies (44%). Cow physiology affected accumulation and excretion, as in primiparous cows, net body burden and milk assimilation efficiencies were higher and lower, respectively, than in multiparous. Liver-specific enrichment was observed in cows and calves (7.0- and 3.2-fold iPCB and dlPCB + PCDD/F TEQ, compared to empty body-based lipid concentrations), whereas iPCBs were also enriched in kidneys (3.1-fold) and muscles (1.5-fold). Consequently, adipose concentrations did not perfectly represent most edible beef tissues. This highlights the essence of integrating the interplay between physicochemical pollutant properties and animal physiology in transgenerational transfer assessments of PCBs and PCDD/Fs.

Modelling supraglacial debris-cover evolution from the single-glacier to the regional scale: an application to High Mountain Asia

Abstract. Currently, about 12 %–13 % of High Mountain Asia’s glacier area is debris-covered, which alters its surface mass balance. However, in regional-scale modelling approaches, debris-covered glaciers are typically treated as clean-ice glaciers, leading to a bias when modelling their future evolution. Here, we present a new approach for modelling debris area and thickness evolution, applicable from single glaciers to the global scale. We derive a parameterization and implement it as a module into the Global Glacier Evolution Model (GloGEMflow), a combined mass-balance ice-flow model. The module is initialized with both glacier-specific observations of the debris' spatial distribution and estimates of debris thickness. These data sets account for the fact that debris can either enhance or reduce surface melt depending on thickness. Our model approach also enables representing the spatiotemporal evolution of debris extent and thickness. We calibrate and evaluate the module on a selected subset of glaciers and apply GloGEMflow using different climate scenarios to project the future evolution of all glaciers in High Mountain Asia until 2100. Explicitly accounting for debris cover has only a minor effect on the projected mass loss, which is in line with previous projections. Despite this small effect, we argue that the improved process representation is of added value when aiming at capturing intra-glacier scales, i.e. spatial mass-balance distribution. Depending on the climate scenario, the mean debris-cover fraction is expected to increase, while mean debris thickness is projected to show only minor changes, although large local thickening is expected. To isolate the influence of explicitly accounting for supraglacial debris cover, we re-compute glacier evolution without the debris-cover module. We show that glacier geometry, area, volume, and flow velocity evolve differently, especially at the level of individual glaciers. This highlights the importance of accounting for debris cover and its spatiotemporal evolution when projecting future glacier changes.

Long-term (~40 years) mass balance appraisal and response of the Patsio glacier, in the Great Himalayan region towards climate change

Comprehensive assessment of the long-term mass balance (40 years) has been performed for Patsio glacier located in Bhaga river basin in the Great Himalayan region. The temperature-index approach has been employed for generating the time-series of annual specific mass balance of 25 years (1993–2018) using Snow and Avalanche Study Establishment (SASE) field observatory data in Patsio region. Results based on observational data showed nearly balanced condition until the year 1999 (+0.03 m.w.e/yr), followed by an accelerated rate of mass loss during the period 2000–2007 (−0.17 m.w.e./yr). This was evident due to the annual rise (+0.7°C) in the temperature and drop (−122 mm) in the amount of precipitation during 2000–2007. A slight drop in the mass balance (−0.14 m.w.e/yr) was observed during the past decade (2008–2018) which was well supported by in-situ climate observations and published geodetic results in the region. The altitudinal distribution of modelled mass balance was observed well in correlation with the glaciological measurements for the periods 2010–2011 and 2011–2012. Further, seasonal sensitivity characteristic (SSC) was developed for the glacier illustrating the dependence of specific mass balance on monthly anomalies in temperature and precipitation. The developed SSC was used with bias-corrected temperature and precipitation estimates from recently released ERA5 reanalysis to reconstruct annual specific mass balance during since 1979s.

Catchment-scale groundwater-flow and recharge paradox revealed from base flow analysis during the Australian Millennium Drought (Mt Lofty Ranges, South Australia)

Catchment-scale recharge and water balance estimates are commonly made for the purposes of water resource management. Few catchments have had these estimates ground-truthed. One confounding aspect is that runoff and soil-water inputs commonly occur throughout the year; however, in climates with strong dry seasons, base flow can be directly sampled. In an experimental catchment in the Mt. Lofty Ranges of South Australia, run-of-stream hydrochemical parameters were monitored. In this Mediterranean climate during the Millennium Drought (2001–2009), the stream was reduced to disconnected groundwater-fed pools. Two groundwater types were identified: (1) high-salinity type from meta-shale bedrock with thick, clayey regolith and (2) low-salinity type from meta-sandstone bedrock with sandy regolith. End-member mixing using silica and chloride concentrations and robust 87Sr/86Sr ratios reveal an apparent groundwater-flow paradox as follows. According to chloride mass balance and spatial distribution of hydrogeological units, the low-salinity groundwater type has seven times more recharge than the high-salinity type. Over the 28-year record, low-salinity groundwater contributed 25% of stream water, whereas high-salinity groundwater contributed 2–5%. During the drought year, however, annual stream flow from the high-salinity groundwater contributed 50%, whereas low-salinity groundwater contributed 18%. High-salinity groundwater dominated dry-season base flow during all years. The paradox can be resolved as follows: The meta-sandstone terrane drains quickly following wet-season recharge and therefore contributes little to dry-season base flow. Conversely, the meta-shale terrane drains slowly and therefore provides stream flow during dry seasons and drought years.

Impact of coastal East Antarctic ice rises on surface mass balance: insights from observations and modeling

Abstract. About 20 % of all snow accumulation in Antarctica occurs on the ice shelves. There, ice rises control the spatial surface mass balance (SMB) distribution by inducing snowfall variability and wind erosion due to their topography. Moreover these ice rises buttress the ice flow and represent ideal drilling locations for ice cores. In this study we assess the connection between snowfall variability and wind erosion to provide a better understanding of how ice rises impact SMB variability, how well this is captured in the regional atmospheric climate model RACMO2 and the implications of this SMB variability for ice rises as an ice core drilling site. By combining ground-penetrating radar (GPR) profiles from two ice rises in Dronning Maud Land with ice core dating, we reconstruct spatial and temporal SMB variations from 1983 to 2018 and compare the observed SMB with output from RACMO2 and SnowModel. Our results show snowfall-driven differences of up to 1.5 times higher SMB on the windward side of both ice rises than on the leeward side as well as a local erosion-driven minimum at the ice divide of the ice rises. RACMO2 captures the snowfall-driven differences but overestimates their magnitude, whereas the erosion on the peak can be reproduced by SnowModel with RACMO2 forcing. Observed temporal variability of the average SMBs, retrieved from the GPR data for four time intervals in the 1983–2018 range, are low at the peak of the easternmost ice rise (∼0.06 mw.e.yr-1), while they are higher (∼0.09 mw.e.yr-1) on the windward side of the ice rise. This implies that at the peak of the ice rise, higher snowfall, driven by orographic uplift, is balanced out by local erosion. As a consequence of this, the SMB recovered from the ice core matches the SMB from the GPR at the peak of the ice rise but not at the windward side of the ice rise, suggesting that the SMB signal is damped in the ice core.

Dynamics of glacier surface velocity and ice thickness for maritime glaciers in the southeastern Tibetan Plateau

Influenced by climate warming, glacial hazards become increasingly frequent after glacier mass loss in the southeastern Tibetan Plateau. Investigations of mountain glacier surface velocities and volume are of great significance. However, no information is given regarding the specific changes in ice flow to climate warming, the spatiotemporal variations of glacier surface velocity and ice thickness are still unclear. In this study, offset tracking was used to measure glacier surface velocity in the southeastern Tibetan Plateau using Landsat TM/ETM+/OLI imagery from 1990 to 2018. And then spatially-distributed glacier ice thickness was estimated from mass-balance distribution data, glacier surface velocities and the principles of ice-flow. Results show that all maritime glaciers velocity in study area experienced a clear regional slowdown from 1991 to 2018, with robust overall trends of −5.26 m a−1 dec−1 (-19.8% dec−1) and 7.16 m a−1 dec−1 (25.2% dec−1) at elevations below and above 4 600 m a.s.l., respectively. Total volume for glaciers with an area of 954.34 km2 was found to be ~ 86.64 ± 11.09 km3 in 2013, corresponding to an average ice thickness of ~ 91 ± 12 m. The average ice thickness thinned by 0.62% a−1 during 2000–2013, and an accelerated melting can be expected in the long term and then glacier volume decreases more rapidly than in the last decades.

Fission product distribution in irradiated safety-tested and non-safety-tested AGR-2 TRISO particles

Two similarly-irradiated TRISO nuclear fuel particles, one safety-tested and one not-safety-tested, were examined by electron probe microanalysis (EPMA) as part of the advanced gas reactor (AGR) fuel development and qualification programme. The primary objective of this study was to determine the effect of high temperature safety testing on the fission product quantification and distribution. Concentration profiles for fission products were collected along two radii in each particle. Fission product mass balance distribution for both particles was determined by computing the material mass per traverse step-size and multiplying by the weight fraction analysis at that position. Results of these analyses suggest that the non-safety-tested particle contains more fission product mass in the centre 300 µm of the kernel than the safety-tested particle; however, for mobile fission products such as Cs, Xe, Te, Sr, Ba, and Pd, the mass moves out further from the particle centre in the safety-tested particle than the non-safety-tested particle. This mass balance approach to TRISO particle analysis is beneficial at elucidating fission product migration in irradiated and safety-tested particles and will be beneficial in applying to other particles to determine potential irradiation damage effects on the fission product distribution and mobility.

Pharmacokinetics, mass balance, tissue distribution, metabolism, and excretion of praliciguat, a clinical-stage soluble guanylate cyclase stimulator in rats.

The pharmacokinetics (PK), metabolism, excretion, mass balance, and tissue distribution of [14C]praliciguat were evaluated following oral administration of a 3‐mg/kg dose in Sprague‐Dawley rats and in a quantitative whole‐body autoradiography (QWBA) study conducted in male Long‐Evans rats. Plasma T max was 1 hour and the t 1/2 of total plasma radioactivity was 23.7 hours. Unchanged praliciguat accounted for 87.4%, and a minor metabolite (N‐dealkylated‐praliciguat) accounted for 7.6% of the total radioactivity in plasma through 48 hours (AUC0‐48). Tissues with the highest exposure ratios relative to plasma were liver, intestines, adrenal gland, and adipose, and those with the lowest values were seminal vesicle, blood, CNS tissues, lens of the eye, and bone. Most of the [14C]praliciguat‐derived radioactivity was excreted within 48 hours after oral administration. Mean cumulative recovery of the administered radioactivity in urine and feces over 168 hours was 3.7% and 95.7%, respectively. Unchanged praliciguat was not quantifiable in urine or bile of cannulated rats; however, based on the total radioactivity in these fluids, a minimum of approximately 82% of the orally administered dose was absorbed. [14C]Praliciguat was metabolized via oxidative and glucuronidation pathways and the most abundant metabolites recovered in bile were praliciguat‐glucuronide and hydroxy‐praliciguat‐glucuronide. These results indicate that praliciguat had rapid absorption, high bioavailability, extensive tissue distribution, and elimination primarily via hepatic metabolism.

Screw-pressed fractions from green forages as animal feed: Chemical composition and mass balances

In colder humid areas, green forages are recognised as high protein yielding crops and have the potential to be a locally grown sustainable protein source. However, without processing, the protein cannot be properly utilised by monogastrics. By screw pressing, a pulp fraction suitable for ruminants and a protein concentrate suitable for monogastrics can be produced. The aim of this study was to analyse the chemical composition of the produced fractions and the mass balance distribution of protein and dry matter (DM) between the fractions. Mass balances are important in order to determine the potential of biorefining of forages as a tool for sustainable feed production.White clover, red clover, lucerne, and perennial ryegrass were separated into a green juice and a fibrous pulp in a screw-press, and a protein concentrate was precipitated from the green juice by heat or acidic precipitation.The pulp and green juice had an average crude protein (CP) concentration of 203 g/kg DM and 249 g/kg DM, respectively. The majority of the DM and the CP from the plant was retained in the fibrous pulp. Protein concentrates from the green juice and pulp contained higher concentrations of the essential amino acids for monogastrics and ruminants compared with unprocessed forage.The results demonstrate that fractions with high protein concentration and balanced amino acid composition can be produced, which combined with a high recovery highlight the potential of both fractions as a protein source for livestock.

Human exposure and mass balance distribution during procymidone application in horticultural greenhouses

The purpose of this study was to analyze the impact of procymidone application in periurban horticultural greenhouses, especially on workers (applicators and assistants) and soil and plastic mulching, when mechanically pressurized application systems were employed. The mean Potential Dermal Exposure (PDE) was measured using the Whole Body Dosimetry technique. The PDE for the applicators was 188 mL h−1 ± 103 mL h−1, and 14.7 mL h−1 ± 6.3 mL h−1 for the assistants. In the first case, the most exposed body sections were the upper right and left (46.8 mL h−1 ± 23.4 mL h−1; 47.0 mL h−1 ± 23.5 mL h−1) and lower (20.8 mL h−1 ± 10.4 mL h−1; 17.3 mL h−1 ± 8.7 mL h−1) legs, while in the case of assistants, hands and legs were the most impacted limbs. Regarding the Margin of Safety (MOS) during the mix and load stage, two of three pesticide preparations resulted unsafe, while for the applicators, six of six spraying operations were unsafe. For the assistants, five of five operations were safe, but three of them were close to the safety limit.Procymidone distribution between drift (0.03% ± 0.07 %), applicator (0.20% ± 0.15 %), polyethylene mulching (8.5% ± 4.5 %) and soil (3.0% ± 1.1 %) was determined with respect to the total pesticide applied. Procymidone soil impact was also evaluated using Eisenia andrei behavioral tests, finding positive correlations between procymidone application and avoidance and reproduction tests.

Implementing an object-based multi-index protocol for mapping surface glacier facies from Chandra-Bhaga basin, Himalaya

Surface glacier facies are superficial expressions of a glacier that are distinguishable based on differing spectral and structural characteristics according to their age and inter-mixed impurities. Increasing bodies of literature suggest that the varying properties of surface glacier facies differentially influence the melt of the glacier, thus affecting the mass balance. Incorporating these variations into distributed mass balance modelling can improve the perceived accuracy of these models. However, detecting and subsequently mapping these facies with a high degree of accuracy is a necessary precursor to such complex modelling. The variations in the reflectance spectra of various glacier facies permit multiband imagery to exploit band ratios for their effective extraction. However, coarse and medium spatial resolution multispectral imagery can delimit the efficacy of band ratioing by muddling the minor spatial and spectral variations of a glacier. Very high-resolution imagery, on the other hand, creates distortions in the conventionally obtained information extracted through pixel-based classification. Therefore, robust and adaptable methods coupled with higher resolution data products are necessary to effectively map glacier facies. This study endeavours to identify and isolate glacier facies on two unnamed glaciers in the Chandra-Bhaga basin, Himalayas, using an established object-based multi-index protocol. Exploiting the very high resolution offered by WorldView-2 and its eight spectral bands, this study implements customized spectral index ratios via an object-based environment. Pixel-based supervised classification is also performed using three popular classifiers to comparatively gauge the classification accuracies. The object-based multi-index protocol delivered the highest overall accuracy of 86.67%. The Minimum Distance classifier yielded the lowest overall accuracy of 62.50%, whereas, the Mahalanobis Distance and Maximum Likelihood classifiers yielded overall accuracies of 77.50% and 70.84% respectively. The results outline the superiority of the object-based method for extraction of glacier facies. Forthcoming studies must refine the indices and test their applicability in wide ranging scenarios.

Calibrated Ice Thickness Estimate for All Glaciers in Austria

Knowledge on ice thickness distribution and total ice volume is a prerequisite for computing future glacier change for both glaciological and hydrological applications. Various ice thickness estimation methods have been developed but regional differences in fundamental model parameters are substantial. Parameters calibrated with measured data at specific points in time and space can vary when glacier geometry and dynamics change. This study contributes to a better understanding of accuracies and limitations of modelled ice thicknesses by taking advantage of a comprehensive data set of in-situ ice thickness measurements from 58 glaciers in the Austrian Alps and observed glacier geometries of three Austrian glacier inventories between 1969 and 2006. The field data are used to calibrate an established ice thickness model to calculate an improved ice thickness data set for the Austrian Alps. A cross-validation between modelled and measured point ice thickness indicates a model uncertainty of 25-31% of the measured point ice thickness. The comparison of the modelled and measured average glacier ice thickness revealed an underestimation of 5% with a mean standard deviation of 15% for the glaciers with calibration data. The apparent mass balance gradient, the primary model parameter accounting for the effects of surface mass balance distribution as well as ice flux, substantially decreases over time and has to be adjusted for each temporal increment to correctly reproduce observed ice thickness. This reflects the general stagnation of glaciers in Austria. We applied optimized apparent mass balance gradients to all glaciers of the latest Austrian glacier inventory and found a volume of 15.9 km³ for the year 2006. The ten largest glaciers account for 25% of area and 35% of total ice volume. An estimate based on mass balance measurements from nine glaciers indicates an additional volume loss of 3.5 ± 0.4 km³ (i.e. 22 ± 2.5%) until 2016. Relative changes in area and volume were largest at glaciers smaller than 1 km², and relative volume changes appear to be higher than relative area changes for all considered time periods.

Surface mass balance of Davies Dome and Whisky Glacier on James Ross Island, north-eastern Antarctic Peninsula, based on different volume-mass conversion approaches

This study presents surface mass balance of two small glaciers on James Ross Island calculated using constant and zonally-variable conversion factors. The density of 500 and 900 kg·m–3 adopted for snow in the accumulation area and ice in the ablation area, respectively, provides lower mass balance values that better fit to the glaciological records from glaciers on Vega Island and South Shetland Islands. The difference between the cumulative surface mass balance values based on constant (1.23 ± 0.44 m w.e.) and zonally-variable density (0.57 ± 0.67 m w.e.) is higher for Whisky Glacier where a total mass gain was observed over the period 2009–2015. The cumulative surface mass balance values are 0.46 ± 0.36 and 0.11 ± 0.37 m w.e. for Davies Dome, which experienced lower mass gain over the same period. The conversion approach does not affect much the spatial distribution of surface mass balance on glaciers, equilibrium line altitude and accumulation-area ratio. The pattern of the surface mass balance is almost identical in the ablation zone and very similar in the accumulation zone, where the constant conversion factor yields higher surface mass balance values. The equilibrium line altitude and accumulation-area ratio determined for the investigated glaciers differ by less than 2m and 0.01, respectively. The annual changes of equilibrium line altitude and the mean values determined over the period 2009–2015 for Whisky Glacier (311 ± 16 m a.s.l.) and Davies Dome (393 ± 18 m a.s.l.) coincide with the values reported from Bahía del Diablo Glacier on Vega Island but differ from the glaciological records on South Shetland Islands.