This study investigated correlations among ram semen quality parameters, testicular vascularity and echotexture. Measurements were performed biweekly for a year, before (BE) and after ejaculation (AE), on seven Chios breed rams [three rams (group A) and four rams (group B) of 2-6 and 9-13years old, respectively], considering breeding (1) and non-breeding period (2). Hemodynamics [Pulsatility Index (PI), Resistive Index (RI)] were evaluated in each testis. Images of testicular parenchyma were analysed to evaluate echogenicity [Mean Value (MV), Contrast (Con), Gray Value Distribution (GVD), Run Length Distribution (RunLD), Long Run Emphasis (LRunEm)]. Semen volume (V), concentration (C), kinetics (CASA), morphology (Sperm Blue), viability (Eosin-Nigrosin) and DNA fragmentation (Acridine Orange) were assessed. In group A/2, semen V was correlated to left testis PI and right testis RI, BE (r=0.4, p=0.013/r=0.6, p<0.001, respectively), and to right testis PI and RI, AE (r=0.5, p<0.001). In A/1/AE, semen C was correlated to left testis PI (r=-0.5, p=0.003). In group A/1/BE, RI of both testes was correlated to total and tail abnormalities (left: r=-0.5, p=0.008/ p=0.004, respectively - right: r=-0.4/r=-0.5, p=0.01/ p=0.008, respectively). In group B/1/BE, sperm tail and total abnormalities were correlated to left testis Con (r=0.5, p=0.02/ p<0.001, respectively), whereas in A/2/BE, they were correlated to left testis LRunEm (r=-0.8/r=-0.7, p<0.001, respectively). In group A/1/BE, DNA damage was correlated to right testis LRunEm (r=-0.6, p<0.001) and tail abnormalities to left testis PI (r=-0.5, p=0.03). In group B/1/AE, right testis LRunEm, GVD, RunLD, were correlated to sperm DNA integrity (r=-0.9, p<0.001). Immotile spermatozoa and viability were correlated to left testis Con in group B/2/AE (r=-0.6/r=0.6, p<0.001) and to right testis MV in group A/1/AE (r=-0.6/r=0.6, p<0.001). In conclusion, alterations in both ram testes blood flow and echotexture seem to be moderately correlated with sperm assessed parameters, depending on season and ram age.