A large number of diatom-based classification systems have been developed worldwide in recent years. These new systems, together with the oldest, emerged on the need to assess the water quality of rivers, but knowledge on possible divergences resulting from their simultaneous application within a territory is limited. This study aimed to compare the ecological status classification provided by conceptually different methodological approaches, of use or potential use within the same region. 402 monitoring samples were collected from Atlantic siliceous streams (NW-Iberian Peninsula) and temporary Mediterranean streams (Balearic Islands, Spain). Two multimetric indices specifically developed for these areas (MDIAT and DIATMIB, respectively) were calculated, as well as the Specific Polluosensitivity Index (IPS). Multimetric indices were more sensitive methods at diagnosing degradation than IPS since they took directly account of abundance (i.e. chlorophyll a in DIATMIB) or indirectly by its proved inverse relationship with Chl a (MDIAT), together with their use of the regional reference diatom community. Alteration gradients were identified in both studied regions based on the distribution of diatoms, with the first axis of distance-based redundancy analyses (dbRDA) being related to nutrient enrichment and organic loads. Threshold Indicator Taxa ANalysis (TITAN) performed on diatoms sampled along environmental (dbRDA axis 1 and phosphate) and biological gradients (as Ecological Quality Ratio scores of classifications), pointed to lower than current Good/Moderate boundaries for phosphate maximum values (e.g. 22.5 and 71.6 μg L−1 for Galicia and the Balearic Islands, respectively) as well as for higher Good/Moderate boundaries for the MDIAT and IPS classifications. A ‘transition group’ of species was classified as sensitive or as tolerant depending on the regional nutrients range. Findings of the present study highlight the need to perform auto-ecological studies to increase the knowledge on regional diatoms and their optimal survival ranges across regions prior to adopt other non-regional diatom indices.