Distribution Of Anthocyanins Research Articles

Research progress of anthocyanins regulating intestinal microorganisms

Anthocyanins are a kind of glycoside derivatives with C6-C3-C6 as the skeleton widely existing in various fruits and vegetables. In addition to giving food its bright color, anthocyanins have a variety of biological health effects on the human body. In this paper, the structure, distribution, and content of anthocyanins and the research progress of anthocyanins and their metabolites on intestinal microbiological regulation in recent years were reviewed in order to provide a theoretical basis for further development of nutritive and healthy food rich in anthocyanins.

Halochromic smart packaging film based on montmorillonite/polyvinyl alcohol-high amylose starch nanocomposite for monitoring chicken meat freshness

Polyvinyl alcohol (PVA) was blended with high amylose starch (HAS) at a ratio of 3:1, and reinforced with montmorillonite (MMT K10) at different concentrations (1, 2, 5, and 7 % w/w of polymers) and anthocyanins (ANT) to develop an active and smart packaging film. MMT addition enhanced the film's mechanical, barrier, thermal, and water resistance properties. Incorporating ANT extracted from roselle calyx into the optimal nanocomposite film (MMT/PVA-HAS II) increased the films' antioxidant, pH-response, and antibacterial properties. FTIR, XRD, and SEM confirmed the intermolecular interactions and even distribution of ANT and MMT in the film matrix. Release rate of ANT was dependent on type of simulant, with higher rate in aqueous solutions compared to alcoholic/fatty food simulants, and cytotoxicity evaluation proved safety of films for food packaging applications. Storage experiments confirmed the potential applicability of the novel halochromic smart film as a promising candidate for monitoring chicken spoilage under abusive storage conditions.

A chromosome-scale genome sequence of Aeonium(Aeonium arboreum 'Velour') provides novel insights into the evolution of anthocyanin synthesis

Anthocyanin glycoside is a water-soluble flavonoid compound that colors plants and aids in stress resistance. The mechanism driving the evolution of the anthocyanin synthesis pathway in plants remains unclear. Aeonium plants are highly regarded as model organisms for studying adaptive evolution. These plants can be categorized into various types, each distinguished by the content and distribution of anthocyanins in their leaves. The categories include red leaves, green leaves, black leaves, yellow leaves, and a classification known as the 'spot brocade series. In this study, we successfully assembled and annotated the genome of cultivar 'Aeonium arboreum 'Velour'' at chromosomal level. The genome size is 1,334.85 Mb containing 18 chromosomes in a single set, with a contig N50 of 23.47 Mb and a Scaffold N50 of 25.07 Mb. Through homology prediction, de novo prediction, and transcriptome prediction, we identified 166,228 coding genes, 161,656 of which were successfully annotated in the database. Comparative genomic analysis revealed that Aeonium arboreum 'Velour' underwent an independent genome-wide replication event after differentiating from Sedum album, Kalanchoe laxiflora, and Kalanchoe fedtschenkoi. It also shared a genome-wide replication event with Sedum album and Kalanchoe laxiflora. Aeonium arboreum 'Velour' exhibits a higher number of multi-copy gene families compared to other species. A total of 5,129 gene families unique to Aeonium arboreum 'Velour' were identified, primarily enriched in various metabolic pathways, including monoterpenoid biosynthesis, sesquiterpene and triterpene biosynthesis, cyanamide acid metabolism, flavonoid and flavonol biosynthesis, phosphonate and phosphinate metabolism, fatty acid degradation, biosynthesis of unsaturated fatty acid, ether lipid metabolism, tyrosine metabolism, and isoflavone biosynthesis according to the KEGG pathway analysis. Aeonium arboreum 'Velour' and Sedum album diversion dates back to approximately 43.11 million years ago during the Paleogene period, marked by the expansion of 2,807 gene families. In contrast, the divergence from Kalanchoe laxiflora and Kalanchoe fedtschenkoi began around 57.28 million years ago, with 219 gene families expanding. GO analysis highlighted that most of the expansion or contraction gene families were predominantly enriched in flower organs, leaf organ development, anthocyanin metabolism regulation, and light energy absorption and utilization. Remarkably, anthocyanin metabolism regulation is enriched to 80 expanded genes, including 36 bHLH transcription factors, possibly functioning as photosensitive pigment interaction factors (PIFs). We speculate that flavonoids play a pivotal role in the adaptation of Aeonium arboreum 'Velour' to environmental stress. Moreover, the evolution of the anthocyanin synthesis pathway is potentially driven by the plant's capability to absorb and utilize light energy, especially in high CO2 and high-temperature settings characteristic of the early Paleogene.

Detection of anthocyanin content in fresh Zijuan tea leaves based on hyperspectral imaging

Anthocyanins are characteristic substances that determine the leaf color and sensory quality of Zijuan tea. The qualitative determination of anthocyanin content in fresh Zijuan tea leaves by artificial vision may lead to the uneven quality of tea products. Hyperspectral images at 400–956 nm were obtained. K-nearest neighbor (KNN) and support vector machine (SVM) models were used to identify the tenderness grade, and partial least squares regression (PLSR) and support vector regression (SVR) models were established based on principal component analysis for prediction. In short, the classification accuracy of the SVM model was better than 90%; variable combination population analysis (VCPA) and variables combination population analysis combined with iterative retained information variable (VCPA-IRIV) can effectively simplify the model and the prediction accuracy is over than 0.92. The best predictive models of total anthocyanins, Cya-3,5-O-diglucoside, Cya-3-O-glucoside, and petunidin were VCPA + SVR, VCPA-IRIV + SVR, VCPA-IRIV + PLSR, and VCPA + PLSR, and the relative percent deviation were 3.233, 2.868, 3.529 and 3.298, respectively. The total anthocyanins were visualized by the spatial distribution of anthocyanins in samples with different tenderness. This study provided a rapid nondestructive method of fresh Zijuan tea leaf tenderness grade and quality components to control the quality of fresh leaf picking of tea with specific leaf color.

Genetic diversity of kale (Brassica oleracea L. var acephala) using agro-morphological and simple sequence repeat (SSR) markers

Kale (Brassica oleracea. var. acephala) is a nutrient-rich green leafy vegetable consumed as food and used in traditional medicine worldwide. An essential step in describing the available genetic resources and ensuring their effective use in breeding programs is to characterize the genetic diversity of available germplasm. In this study, the genetic diversity and structure of 26 kale accessions from South-East Europe were examined using 26 agro-morphological traits collected in the field and 12 simple sequence repeat (SSR) markers. Considerable agro-morphological variability was found in most quantitative (CV = 17.26–42.42%) and qualitative (H' = 0.61–1.79) traits. Multifactorial analysis (MFA) showed that country of origin (33.01%) and morphotype (32.30%) significantly influenced kale diversification. Leaf blade shape (20.62%), leaf incision (19.43%), anthocyanin distribution (16.43%), and leaf colour (15.55%) were the traits that most clearly differentiated accessions. The three common commercial kale cultivars were identified as independent outliers that differed from the other kale accessions in both MFA and UPGMA clustering analysis. The SSR markers were highly informative with 108 alleles and polymorphic information content ranging from 0.395 to 0.856. Strong genetic diversity was detected at the accession level (H' = 0.58) while genetic differentiation was low (Fst = 0.05). Similar to UPGMA clustering, Bayesian clustering suggests that the kale collection can be divided into four clusters with a high degree of admixture and no geographic grouping pattern is apparent. Overall, the study showed that the kale collection studied represents a valuable reservoir of genetic and agro-morphological variability that could be used for future breeding initiatives.

Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection.

Peanut (Arachis hypogaea L.) is an important cash crop worldwide. Compared with the ordinary peanut with pure pink testa, peanut with variegated testa color has attractive appearance and a higher market value. In addition, the variegated testa represents a distinct regulation pattern of anthocyanin accumulation in integument cells. In order to identify the genetic locus underlying variegated testa color in peanut, two populations were constructed from the crosses between Fuhua 8 (pure-pink testa) and Wucai (red on white variegated testa), Quanhonghua 1 (pure-red testa) and Wucai, respectively. Genetic analysis and bulked sergeant analysis sequencing were applied to detect and identify the genetic locus for variegated testa color. Marker-assisted selection was used to develop new variegated testa peanut lines. As a result, all the seeds harvested from the F1 individuals of both populations showed the variegated testa type with white trace. Genetic analysis revealed that the pigmentation of colored region in red on white variegated testa was controlled by a previous reported gene AhRt1, while the formation of white region (un-pigmented region) in variegated testa was controlled by another single genetic locus. This locus, named as AhVt1 (Arachis hypogaea Variegated Testa 1), was preliminary mapped on chromosome 08 through bulked sergeant analysis sequencing. Using a secondary mapping population derived from the cross between Fuhua 8 and Wucai, AhVt1 was further mapped to a 1.89-Mb genomic interval by linkage analysis, and several potential genes associated with the uneven distribution of anthocyanin, such as MADS-box, MYB, and Chalcone synthase-like protein, were harbored in the region. Moreover, the molecular markers closely linked to the AhVt1 were developed, and the new variegated testa peanut lines were obtained with the help of marker-assisted selection. Our findings will accelerate the breeding program for developing new peanut varieties with "colorful" testa colors and laid a foundation for map-based cloning of gene responsible for variegated testa.

FARKLI DEPOLAMA KOŞULLARININ MERLOT ŞARAPLARINDA ANTOSİYANİN PROFİLİ ÜZERİNE ETKİSİ

Grape variety and health, soil and climate, agricultural techniques used, winemaking processes, storage time and temperature are the most important agents in the phenolic content of red wine. In this study, the effects of storage temperature and time on the distribution of anthocyanin compounds, and the total monomeric anthocyanin (TMA) content of Merlot wines were investigated at the beginning of storage and the following quarterly periods. The amount and distribution of delphinidin 3-glucoside, pelargonin 3-glucoside, malvidin 3-glucoside, cyanidin 3-glucoside, and peonidin 3-glucoside anthocyanins in wine samples were defined quantitatively by modifying HPLC method. When the change in TMA content in wines was evaluated, it was determined that there was a decrease of 13.77% at 4-5 °C, 24.28% at 8-10 °C, 43.93% at 12-14 °C, and 66.29% at 18-20 °C compared to the values before storage at the last of 24 months of storage.

Phenotypic Diversity Analysis of the Progeny Variation of a ‘Mosaic Leaf’ Loropetalum chinense var. rubrum Based on Flower Organ Characteristics

Loropetalum chinense var. rubrum is a common landscape plant. Because of the preference for the ‘red leaf’ trait, the ‘flower color’ trait was ignored in breeding, and the genetic diversity of L. chinense var. rubrum was replaced by a few genotypes. Therefore, the potential value of flower color traits was affected, and the genetic diversity was not fully exploited. In this study, we utilized a cultivar with mosaic-colored flowers and leaves and observed 15 phenotypic traits in its flower organs and its progeny, as well as a total of 60 different flower color materials. We analyzed the variation characteristics and diversity of flower colors, and qualitatively described and preliminarily classified flower colors using the ISCC-NBS and CIELab systems to explore the correlation with the flower color phenotype from the distribution and content of anthocyanins. The phenotypic diversity of the flower organs was enriched, and genetic differences in flower color were significant for the L.chinense var. rubrum, with most of the 15 phenotypic traits showing significant positive correlations, and the most critical phenotypes are sepal, petal and nectary color, as well as petal length. According to the CIELab color system and anthocyanin content and distribution characteristics, it is considered more accurate and convenient to classify the color phenotypes of L. chinense var. rubrum flowers into five categories. In this study, for the first time, the flower organ phenotype of L. chinense var. rubrum has been systematically analyzed, and it is explored as a reference method for breeding new cultivars of a diverse range of colors in L. chinense var. rubrum.

PhUGT78A22, a novel glycosyltransferase in Paeonia ‘He Xie’, can catalyze the transfer of glucose to glucosylated anthocyanins during petal blotch formation

BackgroundFlower color patterns play an important role in the evolution and subsequent diversification of flowers by attracting animal pollinators. This interaction can drive the diversity observed in angiosperms today in many plant families such as Liliaceae, Paeoniaceae, and Orchidaceae, and increased their ornamental values. However, the molecular mechanism underlying the differential distribution of anthocyanins within petals remains unclear in Paeonia.ResultsIn this study, we used an intersectional hybrid between the section Moutan and Paeonia, hereafter named Paeonia ‘He Xie’, which has purple flowers with dark purple blotches. After Ultra-high performance liquid chromatography-diode array detector (UPLC-DAD) analysis of blotched and non-blotched parts of petals, we found the anthocyanin content in the blotched part was always higher than that in the non-blotched part. Four kinds of anthocyanins, namely cyanidin-3-O-glucoside (Cy3G), cyanidin-3,5-O-glucoside (Cy3G5G), peonidin-3-O-glucoside (Pn3G), and peonidin-3,5-O-glucoside (Pn3G5G) were detected in the blotched parts, while only Cy3G5G and Pn3G5G were detected in the non-blotched parts. This suggests that glucosyltransferases may play a vital role in the four kinds of glucosylated anthocyanins in the blotched parts. Moreover, 2433 differentially expressed genes (DEGs) were obtained from transcriptome analysis of blotched and non-blotched parts, and a key UDP-glycosyltransferase named PhUGT78A22 was identified, which could use Cy3G and Pn3G as substrates to produce Cy3G5G and Pn3G5G, respectively, in vitro. Furthermore, silencing of PhUGT78A22 reduced the content of anthocyanidin 3,5-O-diglucoside in P. ‘He Xie’.ConclusionsA UDP-glycosyltransferase, PhUGT78A22, was identified in P. ‘He Xie’, and the molecular mechanism underlying differential distribution of anthocyanins within petals was elucidated. This study provides new insights on the biosynthesis of different kinds of anthocyanins within colorful petals, and helps to explain petal blotch formation, which will facilitate the cultivar breeding with respect to increasing ornamental value. Additionally, it provides a reference for understanding the molecular mechanisms responsible for precise regulation of anthocyanin biosynthesis and distribution patterns.

AcMYB1 Interacts With AcbHLH1 to Regulate Anthocyanin Biosynthesis in Aglaonema commutatum.

Aglaonema commutatum is one of the most popular foliage plants with abundant leaf phenotypes; therefore, anthocyanin coloration is a vital economic trait in A. commutatum. However, the molecular mechanisms underlying anthocyanin biosynthesis and its regulation remain unclear. In this study, AcMYB1 and AcbHLH1, transcription factor genes related to an R2R3-myeloblast (MYB) and a basic helix–loop–helix (bHLH), respectively, were isolated from A. commutatum “Red Valentine” and functionally characterized. AcMYB1 and AcbHLH1 were found to interact by Y2H and BiFC assay. AcMYB1 was grouped into the AN2 subgroup and shared high homology with the known regulators of anthocyanin biosynthesis. Gene expression analysis showed that both AcMYB1 and AcbHLH1 have similar expression patterns to anthocyanin structural genes and correlate with anthocyanin distribution in different tissues of A. commutatum. Light strongly promoted anthocyanin accumulation by upregulating the expression of anthocyanin-related genes in A. commutatum leaves. Ectopic expression of AcMYB1 in tobacco remarkably increased anthocyanin accumulation in both vegetative and reproductive tissues at various developmental stages. These results provide insights into the regulation of anthocyanin biosynthesis in A. commutatum and are useful for breeding new A. commutatum cultivars with enhanced ornamental value.

A newlycharacterized allele of ZmR1 increases anthocyanin content in whole maize plant and the regulation mechanism of different ZmR1 alleles.

The novel ZmR1CQ01 allele for maize anthocyanin synthesis was identified, and the biological function and regulatory molecular mechanisms of three ZmR1 alleles were unveiled. Anthocyanins in maize are valuable to human health. The R1 gene family is one of the important regulatory genes for the tissue-specific distribution of anthocyanins. R1 gene allelic variations are abundant and its biological function and regulatory molecular mechanisms are not fully understood. By exploiting genetic mapping and transgenic verification, we found that anthocyanin pigmentation in maize leaf midrib was controlled by ZmR1 on chromosome 10. Allelism test of maize zmr1 EMS mutants confirmed that anthocyanin pigmentation in leaf sheath was also controlled by ZmR1. ZmR1CQ01 was a novel ZmR1 allelic variation obtained from purple maize. Its overexpression caused the whole maize plant to turn purple. ZmR1B73 allele confers anthocyanin accumulation in near ground leaf sheath rather than in leaf midribs. The mRNA expression level of ZmR1B73 was low in leaf midribs, resulting in no anthocyanin accumulation. ZmR1B73 overexpression promoted anthocyanin accumulation in leaf midribs. Loss of exon 5 resulted in ZmR1ZN3 allele function destruction and no anthocyanin accumulation in leaf midrib and leaf sheath. DNA affinity purification sequencing revealed 1010 genes targeted by ZmR1CQ01, including the bz2 in anthocyanin synthesis pathway. RNA-seq analysis showed 55 genes targeted by ZmR1CQ01 changed the expression level significantly, and the expression of genes encoding key enzymes in flavonoid and phenylpropanoid biosynthesis pathways were significantly up-regulated. ZmR1 functional molecular marker was developed. These results revealed the effects of transcriptional regulation and sequence variation on ZmR1 function and identified the genes targeted by ZmR1CQ01 at the genome-wide level.

Relationship between physicochemical characteristics and gene expression in lettuce as a tool for genetic improvement

Alface ( Lactuca sativa L.) is a leafy vegetable worldwide consumed. It is found in its constitution vitamins and minerals, as well as bioactive compounds, such as phenolic compounds, flavonoids and anthocyanins. Since anthocyanins are responsible for the red color of the leaves and in the body prevents the appearance of non-communicable chronic diseases by the fight against free radicals. The lettuce for being present in the diet of the population becomes a promising plant for biofortification with the selection of plants with high levels of anthocyanins. The objective of the present work was to study the quantitative distribution of anthocyanin in leaves of lettuce plants of a segregating F2 population obtained from the initial crossing between two color contrasting parents (green and red).. The color, anthocyanin and flavonoid contents, as well as color correlation with anthocyanin content and the heritability of these characteristics. It was concluded that the anthocyanin content in leaves of lettuce is controlled by more than one gene with partial dominance of the genes that confer higher levels. Intense red coloration can be used as an alternative in lettuce breeding programs to identify superior lettuce genotypes with high anthocyanin content. Transgressive segregation as well as the higher heritability values observed in the studied traits will allow selecting in segregating generations, superior genotypes in accordance with the proposed objectives.

ПЕРСПЕКТИВИ ОТРИМАННЯ АНТОЦІАНОВИХ БАРВНИКІВ ДЛЯ ХАРЧОВОЇ ПРОМИСЛОВОСТІ

The concept of food additives is considered in the article. A special place among food additives belongs to colouring agents used in various branches of food industry. Colouring agents are divided into natural, synthetic and inorganic. Nowadays, the range of synthetic is quite wide against to natural ones. The aim of the work was to summarize scientific and technical information on potentially productive raw materials to get of natural food colouring agents – anthocyanins, analysis of technologies for (raw materials) processing. Anthocyanins colour plant organs in violet, blue, dove-coloured, crimson, and pink. Anthocyanins are powerful antioxidants; they bind oxygen free radicals and prevent from damaging cell membranes. The raw materials, rich in anthocyanins (blackberry, blueberry, elderberry, mulberry, black currant and chokeberry) were chemically studied. Analyzing the obtained data, we can conclude that the mentioned raw materials are not only the colouring agents but appear to be also a source of biologically active substances. As distribution of anthocyanins in fruits is uneven the fruit peel contains, is by 3–7 times more than the juice, it is desirable to take a comprehensive approach to processing of the raw materials. So you can get the juice, to be concentrated later. The received pomace can be extracted: to get coloring substances; dry and obtain powder to; boil soft and cream, in order to obtain a puree or paste. Thus, you can get several products that would act as a dye or dietary supplement. However, when developing natural colouring agents, one shall to remember that the color of natural anthocyanins depends on the structure, the pH of the environment, the formation of complexes with metals, the ability to adsorb on polysaccharides, depends on temperature and light.

Anthocyanin Distribution and Sampling Method in Colored Potato Tubers

Anthocyanin Distribution and Sampling Method in Colored Potato Tubers

Genome-Wide Analysis of MYB10 Transcription Factor in Fragaria and Identification of QTLs Associated with Fruit Color in Octoploid Strawberry.

The genus Fragaria encompass fruits with diverse colors influenced by the distribution and accumulation of anthocyanin. Particularly, the fruit colors of strawberries with different ploidy levels are determined by expression and natural variations in the vital structural and regulatory genes involved in the anthocyanin pathway. Among the regulatory genes, MYB10 transcription factor is crucial for the expression of structural genes in the anthocyanin pathway. In the present study, we performed a genome wide investigation of MYB10 in the diploid and octoploid Fragaria species. Further, we identified seven quantitative trait loci (QTLs) associated with fruit color in octoploid strawberry. In addition, we predicted 20 candidate genes primarily influencing the fruit color based on the QTL results and transcriptome analysis of fruit skin and flesh tissues of light pink, red, and dark red strawberries. Moreover, the computational and transcriptome analysis of MYB10 in octoploid strawberry suggests that the difference in fruit colors could be predominantly influenced by the expression of MYB10 from the F. iinumae subgenome. The outcomes of the present endeavor will provide a platform for the understanding and tailoring of anthocyanin pathway in strawberry for the production of fruits with aesthetic colors.

Anthocyanins: Biosynthesis, Distribution, Ecological Role, and Use of Biostimulants to Increase Their Content in Plant Foods—A Review

In the past century, plant biostimulants have been increasingly used in agriculture as innovative and sustainable practice. Plant biostimulants have been mainly investigated as potential agents able to mitigate abiotic stress. However, few information is available about their ability to influence fruit quality or change fruit phytochemical composition. In particular, very little is known about their effects on anthocyanin synthesis and accumulation. Due to the increasing demand of consumers for healthier foods with high nutraceutical values, this review tries to fill the gap between anthocyanin content and biostimulant application. Here, we elucidate the chemical structure, biosynthetic pathway, plant distribution, and physiological role of anthocyanins in plants. Moreover, we discuss the potential implications for human health derived from the consumption of foods rich in these molecules. Finally, we report on literature data concerning the changes in anthocyanin content and profile after the application of biostimulant products on the most common anthocyanin-containing foods.

Influence of Post-Flowering Climate Conditions on Anthocyanin Profile of Strawberry Cultivars Grown from North to South Europe

The effect of cultivar and environmental variations and their interaction on anthocyanin components of strawberry were assessed for six cultivars grown in five locations from North to South of Europe in two different years. To evaluate the impact of latitude- and altitude-related factors, daily mean (Tmean), maximum (Tmax) and minimum (Tmin) temperature and global radiation accumulated for 3, 5, 10 and 15 days before fruit sampling, was analyzed. In general, fruits grown in the south were more enriched in total anthocyanin and pelargonidin-3-glucoside (pel-3-glc), the most abundant anthocyanin in strawberry. Principal component analysis (PCA) provided a separation of the growing locations within a cultivar due to latitudinal climatic differences, temporary weather changes before fruit collection and cultivation technique. PCA also depicted different patterns for anthocyanin distribution indicating a cultivar specific reaction on the environmental factors. The linear regression analysis showed that pel-3-glc was relatively less affected by these factors, while the minor anthocyanins cyanidin-3-glucoside, cyanidin-3-(6-O-malonyl)-glucoside, pelargonidin-3-rutinoside and pelargonidin-3-(6-O-malonoyl)-glucoside were sensitive to Tmax. The global radiation strongly increased cya-3-mal-glc in ‘Frida’ and pel-3-rut in ‘Frida’ and ‘Florence’. ‘Candonga’ accumulated less pel-3-glc and total anthocyanin with increased global radiation. The anthocyanin profiles of ‘Gariguette’ and ‘Clery’ were unaffected by environmental conditions.

Visualizing the distribution of strawberry plant metabolites at different maturity stages by MALDI-TOF imaging mass spectrometry

This study aimed to visualize differences in the distribution of citric acid, soluble sugars, and anthocyanins in strawberries at four different maturity stages (green to red strawberries) by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF IMS). Results demonstrated citric acid and sugars are evenly distributed in the entire fruit at all maturity stages, while most of anthocyanins are mainly located in the periphery of fruit with increased abundance in red strawberries, indicating a correlation with the colour attributes. Sugar in red strawberries (11.92 brix) increased by two-fold compared to the green ones (6.23 brix). Finally, absolute quantitation of each compound from HPLC analyses support the quantitative results from MALDI-TOF IMS. The results provide a deeper understanding in the changes and distribution of phytochemicals during the growth of strawberries, and demonstrates the usefulness of IMS for plant breeding and postharvest technology.

Study of the Relationship between Leaf Color Formation and Anthocyanin Metabolism among Different Purple Pakchoi Lines.

Purple pakchoi (Brassica rapa ssp. Chinensis) is particularly appreciated due to its high edible quality and ornamental value, but there are few studies on the underlying mechanisms of leaf color formation. To comprehensively assess the differences in purple formation in pakchoi, four lines of pakchoi with different purple leaves were used in this experiment to determine the pigment content and to investigate the distribution and components of anthocyanin using LCMS (Liquid Chromatography Mass Spectrometry) and leaf cross-sections. Moreover, the expression levels of anthocyanin synthesis-related genes in four lines were calculated by qRT-PCR. The results showed that three new purple lines rich in anthocyanin and of high-quality were bred, and the anthocyanin were mainly distributed in both the upper epidermis and lower epidermis of leaves. Thirteen anthocyanin components were separated and identified, all the anthocyanins were acylated and glycosylated cyanidins; the main anthocyanins in purple pakchoi were a diacylated form of cyanidin 3-trans-(feruloyl)diglucoside-5-(malonyl)glucoside. Both the ratio of non-aromatic acylated cyanidin to aromatic acylated cyanidin and the ratio of anthocyanin content to chlorophyll content were responsible for the color formation in different purple pakchoi lines. When the ratio was high, the leaf appeared reddish purple, and when the ratio was low, the leaf appeared deep purple, even blackish purple. The expression level of BrF3H was significantly correlated with the content of anthocyanin through the correlation coefficient, which was speculated to be the main anthocyanin synthesis-related gene resulting in color differences among the four purple pakchoi lines. These results will enhance our understanding for the cultivation of new purple pakchoi varieties.

Comparison of anthocyanin distribution in berries of Haskap (Lonicera caerulea subsp. edulis (Turcz. ex. Herder) Hultén), Miyama-uguisukagura (Lonicera gracilipes Miq.), and their interspecific hybrid using imaging mass spectrometry

Haskap (Lonicera caerulea subsp. edulis), a shrub with violet–blue fruits, is distributed mainly in Hokkaido, Japan. Miyama-uguisukagura (Lonicera gracilipes), a species related to Haskap, produces red fruits. Interspecific hybridization of Miyama-uguisukagura and Haskap was performed to introduce novel characteristics in the resulting hybrids. The shape and color of the interspecific hybrid fruits differed from those of the parent fruits. A comparison of anthocyanin distribution among these three fruit types by imaging mass spectrometry (IMS) revealed the presence of five different anthocyanins. The average cyanidin 3,5-diglucoside and peonidin 3,5-diglucoside intensities in the interspecific hybrid fruit were higher than those of the parent fruits, whereas the average pelargonidin 3-glucoside, cyanidin 3-glucoside, and peonidin 3-glucoside intensities were the highest in Haskap. All anthocyanins were mainly accumulated in the inner and outer skins of Haskap and interspecific hybrid fruits, and in the skin of Miyama-uguisukagura fruits. The order of signal intensities of all anthocyanins among the three fruits was unchanged in different regions. Additionally, a comparison of IMS and LC/MS data from our previous study confirmed the possibility of comparing multiple fruits in the same plate by IMS. Thus, we elucidated anthocyanin distribution patterns of the interspecific hybrid and parent fruits by IMS.