The microwave rotational spectrum of the aromatic 2,3-difluorophenol (2,3-DFP) has been recorded and analyzed in the frequency range of 5–25 GHz using a pulsed-jet Fourier transform microwave spectrometer. Rotational transitions were measured for the parent, the 18O, and all six 13C singly substituted isotopologues in natural abundance as well as the deuterium enriched 2HO-species. In addition to the rotational and quartic centrifugal distortion parameters, the quadrupole coupling constants of the deuterium species were determined. The rotational constants for the parent species are obtained as A = 2313.37516(14) MHz, B = 1797.935693(62) MHz, C = 1011.695171(36) MHz. For the deuterated species, the 2H quadrupole coupling constants are χaa = −0.11693(80) MHz and (χbb−χcc) = 0.4112(14) MHz. The partial rs substitution structure as well as a semi-experimental r0 structure was determined from the rotational constants of the observed isotopologues. Both rs and r0 structures agree well with each other and with MP2/6-311++g(2d, 2p) and B3LYP/6-311++g(2d, 2p) predicted equilibrium structures with the biggest deviation in heavy atom bond lengths being 0.008 Å.