Distinct Cycle Research Articles

Precision Aquaculture Drone Mapping of the Spatial Distribution of Kappaphycus alvarezii Biomass and Carrageenan

The aquaculture of Kappaphycus alvarezii (Kappaphycus hereafter) seaweed has rapidly expanded among coastal communities in Indonesia due to its relatively simple farming process, low capital costs and short production cycles. This species is mainly cultivated for its carrageenan content used as a gelling agent in the food industry. To further assist producers in improving cultivation management and providing quantitative information about the yield, a novel approach involving remote sensing techniques was tested. In this study, multispectral images obtained from a drone (Unoccupied Aerial Vehicle, UAV) were processed to estimate the fresh and carrageenan weights of Kappaphycus at a cultivation site in South Sulawesi. The UAV imagery was geometrically and radiometrically corrected, and the resulting orthomosaics were used for detecting and classifying Kappaphycus using a random forest algorithm. The classification results were combined with in situ measurements of Kappaphycus fresh weight and carrageenan content using empirical relations between the area and weight of fresh seaweed/carrageenan. This approach allowed quantifying seaweed biometry and biochemistry at single cultivation lines and cultivation plot scales. Fresh seaweed and carrageenan weights were estimated for different dates within three distinct cultivation cycles, and the daily growth rate for each cycle was derived. Data were upscaled to a small family-scale farm and a large-scale leader farm and compared with previous estimations. To our knowledge, this study provides, for the first time, an estimation of yield at the scale of cultivation lines by exploiting the very high spatial resolution of drone data. Overall, the use of UAV remote sensing proved to be a promising approach for seaweed monitoring, opening the way to precision aquaculture of Kappaphycus.

The number of distinct cycle lengths in the cycle structure of an automorphism on an abelian group

Let G be a finitely generated free abelian group of rank n, and σ an automorphism of G. The set of distinct lengths of non-zero cycles in the cycle structure of σ is written as S(σ,G). It was proved by Klerk et al. that the structure of an arbitrary automorphism σ of G possesses at most finitely many distinct cycle lengths ([4], Proposition 3.2). A problem arises naturally: How many distinct cycle lengths possibly occur in the cycle structure of an automorphism σ of G. In this article, we prove that |S(σ,G)|≤2m if n=2m is even, or |S(σ,G)|≤2m−1 if n=2m−1 is odd, the automorphism σ for which |S(σ,G)| attains the upper bound is characterized definitely.

African ZIKV lineage fails to sustain infectivity in an in vitro mimetic urban cycle.

Zika virus (ZIKV) is an arbovirus maintained in nature in two distinct cycles of transmission: urban and sylvatic. Each cycle includes specific vertebrate and invertebrate hosts, and through alternate infections, a conserved consensus sequence is maintained that might vary depending on the cycle. The current study aimed to investigate the ability of ZIKVAF and ZIKVBR to maintain an infectious cycle by alternating passages in cells mimicking the urban (UC) and semi-sylvatic (SC) cycles. The complete genome of the original inoculum and the last passages for each cycle were sequenced by Sanger. Ten passages were performed, as planned, for ZIKVBR UC, ZIKVAF SC, and ZIKVBR SC. ZIKVBR SC showed significant variation in viral titers along the passages, suggesting that the virus is not well adapted to the non-human primate host. ZIKVAF passage in UC was abrogated in the third passage, showing the inability of the African lineage to sustain cycles in human cells, suggesting a low capacity to establish an urban cycle. Several mutations were found in both strains along the passages, but not occurring at equivalent positions. Further studies are needed to elucidate whether any of these specific mutations affect viral fitness. ZIKV strains behave differently in artificial transmission cycles in vitro: Brazilian ZIKV was able to establish urban and semi-sylvatic cycles in vitro. African ZIKV proved unable to cycle among human and mosquito cells and is compatible only with the semi-sylvatic cycle. The main mutations arose in the NS2A region after artificial transmission cycles for both ZIKV strains but not at equivalent positions.

Analyzing the Combined Effect of Multiple Environmental Factors on Fish Distribution, by Means of the Mixed Distribution-Decomposition Approach, as Illustrated by the East China Sea Hairtail.

An organism's habits are formed primarily as a result of environmental circumstances. Analyzing an organism's habits and examining their causes requires a thorough understanding of the peculiarities of an organism's living environments. Analyzing the environmental factors necessary for an organism's survival is a crucial component of studying how the environment and biology interact. The favorable temperature range for the species has been discovered in previous investigations of the hairtails' main water temperature distribution range, covering both the water regions with and without the hairtails. It is implied that there may be other elements besides water temperature that also affect dispersion. The hairtail, though, is still the subject of the study. To investigate and confirm the corollaries, salinity and water depth were added as variables. We observed that the intersection of the main ranges of two environmental factors, as well as the main hairtail range of interest, were greatly reduced when compared to a single factor range; the sum of the three factors will further increase the reduction. The primary cause is that the main range of hairtail relative to each factor is incomplete, and the target bodies specified by various factors are very varied. To further investigate the factors that affect the distribution of the organism's active areas, a comparison between aggregated and non-aggregated waters relative to one factor can be done in the next step. A good sequence of environmental elements, namely temperature>salinity>water depth, is obtained in the above analysis procedure by comparing the accuracy and significance of each factor for the primary range of the hairtail. Additionally, it was noted that the main population of the hairtail covered different areas depending on the season, with less coverage in spring and autumn and greater coverage in summer and winter. The main part of the hairtail population also tended to be distributed closer to the coast in summer and winter, and farther offshore in spring and autumn. These seasonal variations may be related to the two distinct reproductive cycles of hairtail, occuring in spring and autumn.

P–T–t conditions of Early Palaeozoic low‐P high‐T granulite facies metamorphism in the southern Truong Son Belt, Central Vietnam

AbstractHigh‐grade metamorphic rocks are widely exposed along the SE–NW‐ to E–W‐trending shear zones in the Truong Son Belt, Central Vietnam, but few petrological studies have been conducted in this area. Herein, we report the occurrence of mylonitized granulites that crop out along the Dai Loc shear zone in the southernmost Truong Son Belt. Detailed petrographic analysis, geochemistry and P–T–t estimates of the evolution of two granulite samples are presented to elucidate the formation processes of these high‐grade metamorphic rocks. The results indicate that the rocks underwent two distinct metamorphic cycles. The first cycle (M1) is characterized by coarse‐grained granulite mineral assemblages, defining a tight clockwise P–T path with near‐isobaric heating to a near ultrahigh‐temperature peak at low pressure, followed by cooling. The prograde mineral assemblage (M1a) is indicated by inclusions of cordierite + sillimanite + biotite + quartz + spinel ± plagioclase in coarse‐grained garnet, orthopyroxene and cordierite. The mineral assemblage of garnet + orthopyroxene + cordierite + plagioclase + K‐feldspar + ilmenite + melt ± biotite (M1b) defines the peak P–T conditions of 5.3–6.3 kbar and 850–920°C. Post‐peak cooling (M1c) is marked by the formation of quartz + biotite symplectites around garnet and orthopyroxene. The second cycle involved medium‐pressure amphibolite facies metamorphism (M2), characterized by domainal development of fine‐grained kyanite‐bearing mineral associations. Petrographic observations indicate that these fine‐grained associations were formed during mylonitization. Zircon U–Pb dating reveals that the timing of granulite facies metamorphism appears to be coeval with the intrusion of a post‐collisional granitoid at 430–410 Ma. Granulite facies metamorphism and crustal melting were probably driven by asthenospheric mantle upwelling triggered by slab breakoff during the Early Palaeozoic. Considering previous structural and geochronological studies, the second metamorphic event likely occurred during the Triassic Indosinian orogeny.

Inter-annual sea level change and transgression along a barrier Island coast

On the decadal time scale of coastal planning and management, relative sea-level rise is usually considered a trend or constant rate rather than a dynamic, time varying influence on coastal change. However, the coastal ocean sea level along the east coast of Florida and coastal regions further north is, to a first order, controlled at the interannual and decadal time scale by the dynamics of North Atlantic circulation and Gulf Stream (GS) flow, Every coastal region has its own distinctive annual sea-level cycle, and longer-term intra- and inter-decadal sea-level oscillations. This paper addresses the influence of the strong Florida coastal ocean sea-level signal on topographic evolution of the shoreface, coastal sediment budgets, and potential for coastal transgression at the decadal time scale.

Identification of cell division cycle protein 20 in various forms of acute and chronic kidney injury in mice.

Tubular epithelial cell fate following exposure to various types of injurious stimuli can be decided at distinct cell cycle checkpoints. One such checkpoint occurs during mitosis, known as the spindle assembly checkpoint, and is tightly regulated through the actions of cell division cycle protein 20 (CDC20). Due to our paucity of knowledge about the role of CDC20 in the kidney, the present study was designed to investigate the expression levels and distribution of CDC20 within the kidney and how pharmacological inhibition of CDC20 function affects kidney recovery using various rodent models of kidney injury. CDC20 is normally detected in distal tubules, but upon injury by either cisplatin administration or ureter obstruction, CDC20 accumulation is considerably elevated. Blockade of CDC20 activity using a selective pharmacological inhibitor, Apcin, lowered serum creatinine, tubular damage, and DNA injury following acute kidney injury compared with vehicle-treated mice. In unilateral ureteral obstruction, Apcin reduced tissue kidney injury molecule-1 levels, sirius red staining, and tubulointerstitial α-smooth muscle actin staining in the tissue. The findings in the present study demonstrated that elevations in CDC20 levels in the kidney are associated with kidney injury and that inhibition of CDC20 can alleviate and reverse some of the pathological effects on the architecture and function of kidney.NEW & NOTEWORTHY To our knowledge, this is the first study to characterize the expression and localization of cell division cycle 20 protein (CDC20) in normal and acute, and chronically injured kidneys. Tubular epithelial cell damage was markedly reduced through the administration of a selective inhibitor of CDC20, Apcin. This study provides new evidence that CDC20 can be induced in damaged kidney cells and negatively impact the recovery of the kidney following acute kidney injury.

An Update on Current Antiviral Strategies to Combat Human Cytomegalovirus Infection.

Human cytomegalovirus (HCMV) remains an essential global concern due to its distinct life cycle, mutations and latency. As HCMV is a herpesvirus, it establishes a lifelong persistence in the host through a chronic state of infection. Immunocompromised individuals are at risk of significant morbidity and mortality from the virus. Until now, no effective vaccine has been developed to combat HCMV infection. Only a few antivirals targeting the different stages of the virus lifecycle and viral enzymes are licensed to manage the infection. Therefore, there is an urgent need to find alternate strategies to combat the infection and manage drug resistance. This review will provide an insight into the clinical and preclinical antiviral approaches, including HCMV antiviral drugs and nucleic acid-based therapeutics.

Diurnal MCSs Precede the Genesis of Tropical Cyclone Mora (2017): The Role of Convectively Forced Gravity Waves

Abstract A novel high-resolution regional reanalysis is used to investigate the mesoscale processes that preceded the formation of Tropical Cyclone (TC) Mora (2017). Both satellite observations and the regional reanalysis show early morning mesoscale convective systems (MCSs) persistently initiated and organized in the downshear quadrant of the preexisting tropical disturbance a few days prior to the genesis of TC Mora. The diurnal MCSs gradually enhanced the meso-α-scale vortex near the center of the preexisting tropical disturbance through vortex stretching, providing a vorticity-rich and moist environment for the following burst of deep convection and enhancement of the meso-β-scale vortex. The regional reanalysis shows that the gravity waves that radiated from afternoon convection over the northern coast of the Bay of Bengal might play an important role in modulating the diurnal cycle of pregenesis MCSs. The diurnal convectively forced gravity waves increased the tropospheric stability, reduced the column saturation fraction, and suppressed deep convection within the preexisting tropical disturbance from noon to evening. A similar quasi-diurnal cycle of organized deep convection prior to TC genesis has also been observed over other basins. However, modeling studies are needed to conclusively demonstrate the relationships between the gravity waves and pregenesis diurnal MCSs. Also, whether diurnal gravity waves play a similar role in modulating the pregenesis deep convection in other TCs is worth future investigations. Significance Statement Tropical cyclogenesis is a process by which a less organized weather system in the tropics develops into a tropical cyclone (TC). Observations indicate that thunderstorms occurring prior to the tropical cyclogenesis often show a distinct quasi-diurnal cycle, while the related physical mechanisms are still unclear. In this study, we used a novel high-resolution dataset to investigate the diurnal thunderstorms occurring prior to the genesis of TC Mora (2017). We find that the pregenesis diurnal thunderstorms played a crucial role in spinning up the circulation of the atmosphere and provided a favorable environment for the rapid formation of Mora. It is likely that gravity waves emitted by afternoon thunderstorms over the inland region were responsible for regulating the diurnal variation of pregenesis thunderstorms over the ocean.

Automated acoustic detection of river herring (Alewife and Blueback Herring) spawning activity

AbstractObjectiveWe used passive acoustic monitoring (PAM) and automatic detection of spawning splashes to examine the timing and environmental drivers of spawning in river herring (Alewife Alosa pseudoharengus and Blueback Herring A. aestivalis).MethodsAcoustic recordings of spawning splashes were collected from March to May 2021 in the Choptank River, Maryland, using an AudioMoth recorder. Recordings were analyzed using a random forest model on the Rainforest Connection ARBIMON platform to determine hourly presence–absence of splashes.ResultAt a seasonal scale, our results suggested two peaks in spawning activity: early March and mid‐April, corresponding with the known phenologies of Alewife and Blueback Herring. Hourly patterns in spawning activity suggested distinct diel cycles, with spawning most concentrated at dawn. In contrast, sonar fish counts collected for 1 week during the season indicated that migration occurred throughout daylight hours. We also found a potential relationship between spawning activity and the presence of great blue herons Ardea herodias.ConclusionOverall, our results demonstrate that PAM can be an efficient and affordable method for studying the spawning ecology of anadromous fish.

The impact and outcomes of cancer-macrophage fusion

BackgroundCancer’s hallmark feature is its ability to evolve, leading to metastasis and recurrence. Although genetic mutations and epigenetic changes have been implicated, they don’t fully explain the leukocytic traits that many cancers develop. Cell fusion between cancer and somatic cells, particularly macrophages, has been suggested as an alternative pathway for cancer cells to obtain new traits by acquiring exogenous genetic material.MethodsThis study aims to investigate the potential biological outcomes of tumor-myeloid cell fusion by generating tumor-macrophage hybrid cells. Two clones with markedly different tumorigenicity were selected, and RNA-seq was used to compare their RNA expressions with that of the control cells. Based on the results that the hybrid cells showed differential activation in several upstream regulator pathways that impact their biological behaviors, the hybrid cells’ abilities to recruit stromal cells and establish angiogenesis as well as their cell cycle distributions were investigated through in vitro and in vivo studies.ResultsAlthough both hybrid clones demonstrated p53 activation and reduced growth rates, they exhibited distinct cell cycle distributions and ability to grow in vivo. Notably, while one clone was highly tumorigenic, the other showed little tumorigenicity. Despite these differences, both hybrid clones were potent environmental modifiers, exhibiting significant abilities to recruit stromal and immune cells and establish angiogenesis.ConclusionsThe study revealed that tumor-somatic cell fusion is a potent environmental modifier that can modulate tumor survival and evolution, despite its relatively low occurrence. These findings suggest that tumor-somatic cell fusion could be a promising target for developing new cancer therapies. Furthermore, this study provides an experimental animal platform to investigate cancer-myeloid fusion and highlights the potential role of tumor-somatic cell fusion in modulating the tumor environment.

Predicting the REIT Corporate Life Cycle Phase on a Financial Accounting Basis

This study investigates the effect of financial–accounting variables on a firm’s distinct life cycle phase. The study concentrates on the real estate sector and uses data of publicly listed REITs which are traded in the European market. To assess the empirical argument, a multinomial panel logit model is employed. The empirical results show that leverage, dividend distribution, the size and the sales variable have significant predictive power over the corporate life cycle classification of an REIT. Higher leverage is associated with an elevated probability of the REIT being classified in the early stage of its corporate life cycle, as opposed to the maturity stage. Positive leverage variation is also a significant predictor of the REIT being categorized in the shake–out stage, while lower dividend distribution, negative sales and size variation are all associated with an increased probability of the REIT being classified in the decline stage, rather than in the maturity phase.

Characteristics of Coastal Low-Level Jets in the Boundary Layer of the Pearl River Estuary

It is well-known that coastal low-level jets (CLLJs) play an essential role in transporting heat, water vapor and pollutants. However, the CLLJ characteristics in the Pearl River Estuary have not been deeply revealed due to the lack of long-term observations. Based on the long-term observations from a wind lidar, we analyze the primary climatic characteristics of the CLLJs in the Pearl River Estuary and investigate their relationships with large-scale and local-scale synoptic systems. The results show that the CLLJs mainly appear during the flood season, with the most occurrence in May. The CLLJ occurrence during the flood season is mainly influenced by the large-scale north–south pressure gradient driven by the western Pacific subtropical high and terrestrial low-pressure systems. The occurrence of the CLLJs exhibits a distinct diurnal cycle with two different peaks in different seasons. One peak appears at nighttime, mainly during non-flood seasons. The other appears in the afternoon, mainly during the flood season. In the non-flood seasons, under the influence of cold air, the inertial oscillations triggered by the land–sea thermal contrast lead to CLLJ onset at nighttime in the Pearl River Estuary. During the flood season, the strong near-surface pressure gradient contributes to CLLJ onset in the afternoon, while the topography (blocking and passing) is more conducive to the occurrence of the CLLJs in the Pearl River Estuary. These findings reveal the formation mechanisms of the CLLJs over the Pearl River Estuary, thus providing a basis for further understanding the precipitation in the Pearl River Estuary and the occurrence of the CLLJs in other coastal areas with complex mountain ranges.

Ins and Outs of Rocker Switch Mechanism in Major Facilitator Superfamily of Transporters.

The major facilitator superfamily (MFS) of transporters consists of three classes of membrane transporters: symporters, uniporters, and antiporters. Despite such diverse functions, MFS transporters are believed to undergo similar conformational changes within their distinct transport cycles, known as the rocker-switch mechanism. While the similarities between conformational changes are noteworthy, the differences are also important since they could potentially explain the distinct functions of symporters, uniporters, and antiporters of the MFS superfamily. We reviewed a variety of experimental and computational structural data on a select number of antiporters, symporters, and uniporters from the MFS family to compare the similarities and differences of the conformational dynamics of three different classes of transporters.

Functional specialization of short-lived and long-lived macrophage subsets in human tonsils.

Macrophages play a central role in tissue homeostasis and host defense. However, the properties of human macrophages in non-diseased tissues remain poorly understood. Here, we characterized human tonsil macrophages and identified three subsets with distinct phenotype, transcriptome, life cycle, and function. CD36hi macrophages were related to monocytes, while CD36lo macrophages showed features of embryonic origin and CD36int macrophages had a mixed profile. scRNA-seq on non-human primate tonsils showed that monocyte recruitment did not pre-exist an immune challenge. Functionally, CD36hi macrophages were specialized for stimulating T follicular helper cells, by producing Activin A. Combining reconstruction of ligand-receptor interactions and functional assays, we identified stromal cell-derived TNF-α as an inducer of Activin A secretion. However, only CD36hi macrophages were primed for Activin A expression, via the activity of IRF1. Our results provide insight into the heterogeneity of human lymphoid organ macrophages and show that tonsil CD36hi macrophage specialization is the result of both intrinsic features and interaction with stromal cells.

Usefulness of cultivar-level calibration of AquaCrop for vegetables depends on the crop and data availability.

As a result of climate change, climatic extremes are expected to increase. For high-value crops like vegetables, irrigation is a potentially economically viable adaptation measure in western Europe. To optimally schedule irrigation, decision support systems based on crop models like AquaCrop are increasingly used by farmers. High value vegetable crops like cauliflower or spinach are grown in two distinct growth cycles per year and, additionally, have a high turnover rate of new varieties. To successfully deploy the AquaCrop model in a decision support system, it requires a robust calibration. However, it is not known whether parameters can be conserved over both growth periods, nor whether a cultivar dependent model calibration is always required. Furthermore, when data are collected from farmers' fields, there are constraints in data availability and uncertainty. We collected data from commercial cauliflower and spinach fields in Belgium in 2019, 2020 and 2021 during different growing periods and of different cultivars. With the use of a Bayesian calibration, we confirmed the need for a condition or cultivar specific calibration for cauliflower, while for spinach, splitting the data per cultivar or pooling the data together did not improve uncertainty on the model simulations. However, due to uncertainties arising from field specific soil and weather conditions, or measurement errors from calibration data, real time field specific adjustments are advised to simulations when using AquaCrop as decision support tool. Remotely sensed or in situ ground data may be invaluable information to reduce uncertainty on model simulations.

A non-canonical sensing pathway mediates Plasmodium adaptation to amino acid deficiency

Eukaryotes have canonical pathways for responding to amino acid (AA) availability. Under AA-limiting conditions, the TOR complex is repressed, whereas the sensor kinase GCN2 is activated. While these pathways have been highly conserved throughout evolution, malaria parasites are a rare exception. Despite auxotrophic for most AA, Plasmodium does not have either a TOR complex nor the GCN2-downstream transcription factors. While Ile starvation has been shown to trigger eIF2α phosphorylation and a hibernation-like response, the overall mechanisms mediating detection and response to AA fluctuation in the absence of such pathways has remained elusive. Here we show that Plasmodium parasites rely on an efficient sensing pathway to respond to AA fluctuations. A phenotypic screen of kinase knockout mutant parasites identified nek4, eIK1 and eIK2—the last two clustering with the eukaryotic eIF2α kinases—as critical for Plasmodium to sense and respond to distinct AA-limiting conditions. Such AA-sensing pathway is temporally regulated at distinct life cycle stages, allowing parasites to actively fine-tune replication and development in response to AA availability. Collectively, our data disclose a set of heterogeneous responses to AA depletion in malaria parasites, mediated by a complex mechanism that is critical for modulating parasite growth and survival.

Basic Methods of Cell Cycle Analysis.

Cellular growth and the preparation of cells for division between two successive cell divisions is called the cell cycle. The cell cycle is divided into several phases; the length of these particular cell cycle phases is an important characteristic of cell life. The progression of cells through these phases is a highly orchestrated process governed by endogenous and exogenous factors. For the elucidation of the role of these factors, including pathological aspects, various methods have been developed. Among these methods, those focused on the analysis of the duration of distinct cell cycle phases play important role. The main aim of this review is to guide the readers through the basic methods of the determination of cell cycle phases and estimation of their length, with a focus on the effectiveness and reproducibility of the described methods.

Diurnal Characteristics in Summer Water Vapor Budget and Transport over the Tibetan Plateau

Using the ERA5 reanalysis dataset during the period 1979–2019, the diurnal variation in summer water vapor budget (Bt) over the Tibetan Plateau (TP) is investigated in this study. It is found that the TP Bt shows a distinct diurnal cycle. It tends to increase in the morning, reaches a peak in the afternoon, and falls to a minimum in the early morning. The diurnal variations in four boundary water vapor budgets of the TP contribute to the growth in the TP Bt from the early morning to the afternoon, of which the western and eastern boundaries are more important. To understand the reasons for the diurnal variations in boundary water vapor budgets, the temporal evolutions of water vapor transports and relevant circulations at the four boundaries are examined. The results show that the temporal evolutions of water vapor transports and budgets at the four boundaries are essentially regulated by the changes in the orographic thermodynamic effect. Specifically, rapid and strong warming (cooling) on the TP slopes generates anomalous water vapor inputs (outputs) by anomalous upslope (downslope) flows during the daytime (nighttime). At the southern and western boundaries, apart from the terrain effects, the diurnal variation in the Indian southerly monsoon also has an effect on the changes in water vapor budgets by modulating the water vapor input towards the TP below 700 hPa. At the northern and eastern boundaries, under the orographic thermodynamic effects, low-level water vapor transports towards the TP accompanying by plateau-scale vertical circulations, exist significant diurnal variations and thereby adjust the boundary water vapor budgets. In this study, it is also found that the deviated water vapor flux vectors over the TP present a daily clockwise rotation, which mainly results from the diurnal variation in wind below 450 hPa. In addition, the largest amount of precipitation over the TP occurs 2–3 h after the Bt peak.

Application of Game Theory Analysis in E-commerce Platform Pricing

The impact of pricing strategy on the profitability of e-commerce platform is studied. First of all, based on the e-commerce platform, considering the interests of the platform, the seller and the buyer in the transaction process, it is of practical and theoretical significance to explore the strategic choice of e-commerce platform pricing from the perspective of game theory. Then, through research, it is found that the operation of the e-commerce platform has distinct life cycle characteristics, that is, the initial use of various subsidy strategies to attract the entry of sellers and buyers, and the medium-term use of asymmetric pricing strategies combined with external response and demand elasticity to ensure the effectiveness of the platform. Finally, when formulating pricing strategies, e-commerce platforms should actively implement the strategy of market-oriented operation, reduce the intervention of sellers and buyers, and ensure the stable development of e-commerce platforms.