A number of cytogenetic methods have been used to duplicate specific chromosomal segments in maize. However, most of these techniques do not produce heritably stable duplications. The absence of stable inheritance for a duplication means that stock maintenance is difficult and potential agricultural applications cannot be pursued. Two methods for producing stable segmental duplications in maize have been previously proposed. Both methods utilize translocations between the maize B chromosome and standard chromosomes (B–A translocations). One technique duplicates proximal chromosomal segments, while the other duplicates distal regions. The proximal-duplication method was tested earlier. The distal-duplication technique is analyzed here. Two homozygous distal duplications were constructed and tested for gametic transmission. The duplications were found to be heritably stable within the limits of the genetic and cytological tests that were employed.Key words: B chromosome, maize, duplication.