Dissolved Nitrogen Research Articles

Response of runoff N and P concentrations, losses, and stoichiometry to rainfall amount category, multisource fertilization, and straw return in sloping croplands

Nitrogen (N) and phosphorus (P) losses via runoff from sloping croplands are a major threat to the degradations of soil fertility and water quality worldwide. Rainfall amount category and agricultural practices, such as multisource fertilization and straw return, are key factors regulating nutrient loss via runoff in sloping croplands. Yet, the impacts of these factors on N and P losses via runoff remain elusive. This study investigated the responses of N and P in runoff to rainfall amount category, multisource fertilization, and the combination of multisource fertilization and straw return in sloping cropland with a radish and maize rotation during multiple natural rainfall events between 2019 and 2021. Three agricultural treatments (three replications for each), i.e., local traditional fertilization (CK), multisource fertilization (T1), and a combination of T1 and straw return (T2), were applied on nine 8 m × 3 m plots. The concentrations and losses of total nitrogen (TN), total phosphorus (TP), dissolved nitrogen (DN), dissolved phosphorus (DP), particulate nitrogen (PN) and particulate phosphorus (PP) and their paired stoichiometries in runoff were determined. The runoff depth during rainstorms was similar to that in large rainstorms and was 191.1 % and 178.7 % higher than in moderate and heavy rains, respectively (p < 0.05). The PN concentration was the highest during moderate rain, and the highest concentrations of TP and DP were observed during large rainstorms (p < 0.05). Rainstorms and large rainstorms produced higher runoff losses for PN, TP, DP, and PP than for other rainfall amount categories (p < 0.05). Compared to CK, the runoff depth was marginally reduced by 4.2 % and 12.7 % in T1 and T2, respectively, and the concentrations and losses of N and P in runoff also slightly increased in T1 and T2. The DN and DP losses accounted for 70.5 % and 59.8 % of the TN and TP losses across all treatments, respectively, indicating that the dissolved forms played a dominant role in nutrient loss via runoff. TN:TP (77:1) in runoff was higher than the Redfield ratio (16:1) across all treatments, implying a stoichiometric potential for P depletion in the runoff. Our results highlight the potential risk of N and P losses in dissolved form via runoff regulated by rainfall, multisource fertilization and straw return in sloping croplands.

Post-drainage vegetation, microtopography and organic matter in Arctic drained lake basins

Wetlands in Arctic drained lake basins (DLBs) have a high potential for carbon storage in vegetation and peat as well as for elevated greenhouse gas emissions. However, the evolution of vegetation and organic matter is rarely studied in DLBs, making these abundant wetlands especially uncertain elements of the permafrost carbon budget. We surveyed multiple DLB generations in northern Alaska with the goal to assess vegetation, microtopography, and organic matter in surface sediment and pond water in DLBs and to provide the first high-resolution land cover classification for a DLB system focussing on moisture-related vegetation classes for the Teshekpuk Lake region. We associated sediment properties and methane concentrations along a post-drainage succession gradient with remote sensing-derived land cover classes. Our study distinguished five eco-hydrological classes using statistical clustering of vegetation data, which corresponded to the land cover classes. We identified surface wetness and time since drainage as predictors of vegetation composition. Microtopographic complexity increased after drainage. Organic carbon and nitrogen contents in sediment, and dissolved organic carbon (DOC) and dissolved nitrogen (DN) in ponds were high throughout, indicating high organic matter availability and decomposition. We confirmed wetness as a predictor of sediment methane concentrations. Our findings suggest moderate to high methane concentrations independent of drainage age, with particularly high concentrations beneath submerged patches (up to 200 μmol l−1) and in pond water (up to 22 μmol l−1). In our DLB system, wet and shallow submerged patches with high methane concentrations occupied 54% of the area, and ponds with high DOC, DN and methane occupied another 11%. In conclusion, we demonstrate that DLB wetlands are highly productive regarding organic matter decomposition and methane production. Machine learning-aided land cover classification using high-resolution multispectral satellite imagery provides a useful tool for future upscaling of sediment properties and methane emission potentials from Arctic DLBs.

Impact of Intercropping on Nitrogen and Phosphorus Nutrient Loss in Camellia oleifera Forests on Entisol Soil

Soil and water loss represent a significant environmental challenge in purple soil cropland in China. However, the quantity and mechanism of nutrient loss from purple soil remain unclear. To understand water and soil conservation and address nitrogen (N) and phosphorus (P) mitigation in Camellia oleifera forest stands on purple soil slope farmland, this study aimed to explore the resistance control effect of forest stands on N and P loss in such agricultural landscapes. In the study, a runoff plot experiment was conducted in purple soil slope farmland. The experiment included three distinct treatments: intercropping of oil tea (Camellia oleifera) and ryegrass (Lolium perenne L.), Camellia oleifera monoculture, and barren land served as the control treatment (CK). Water samples were collected and analyzed from the soil surface runoff and the middle soil layer at a depth of 20 cm (interflow) in three treatment plots under natural rainfall conditions in 2023. Various nutrient components, including total nitrogen (TN), dissolved nitrogen (DN), nitrate nitrogen (NO3−-N), ammonium nitrogen (NH4+-N), particulate nitrogen (PN), total phosphorus (TP), dissolved phosphorus (DP), phosphate (PO4+-P), and particulate phosphorus (PP), were measured in the water samples. The results indicated that intercropping effectively mitigated the loss of various forms of N and P in both surface runoff and interflow within purple soil slope farmland. Compared to the CK, the ryegrass intercropping reduced TN and TP loss by 29.3%–37.3% and 25.7%–38.9%, respectively. The ryegrass intercropping led to a decrease in the average total loss of TN, DN, NO3—N, and NH4+-N by 63.0, 24.3, 4.5, and 6.8 g/ha, corresponding to reductions of 33.3%, 47.6%, 58.3%, and 49.1%, respectively, compared to the CK. The average total loss of TP, DP, and PP decreased by 4.4, 1.8, and 1.4 g/hm2 in the intercropping, reflecting reductions of 32.3%, 31.3%, and 31.1%, respectively. The most significant proportion was observed in PN and PP within the runoff water solution, accounting for 53.3%–74.8% and 56.9%–61.0% of the TN and TP, respectively. These findings establish a foundation for purple soil and water conservation. The research provides valuable insights for land management and policymakers in developing erosion prevention and control programs for sloping cultivated land with Camellia oleifera forests in purple soils. Additionally, it offers guidance for soil and water conservation and prevention of surface source pollution in purple soil regions.

Different Quality Classes of Decomposing Plant Residues Influence Dissolved Organic Matter Stoichiometry Which Results in Different Soil Microbial Processing

The influence of the quantities and ratios of dissolved organic carbon (DOC) and dissolved nitrogen (DN) generated by different chemical quality classes of organic residues on soil microbial processes in the decomposition process is not well understood. If the DOC-to-DN ratio (hereafter, ratio) of the substrate is close to that of the microbial C-to-N ratio, then the DOC-and-DN stoichiometry of the substrate is balanced, resulting in enhanced microbial processing, i.e., carbon use efficiency (CUE). Uncertainty exists about the influence of DN and the DOC-to-DN ratio on CUE, particularly in high-quality class (high nitrogen) residue-treated soils. A long-term field experiment was used to explore the effect of the annual application of residues of different quality classes on decomposition processes, focusing on the effects of DOC, DN, and the ratio on the microbial metabolic quotient (qCO2), which is the inverse of CUE. DOC and DN were extracted from soils during the 13th year of the experiment. Soils treated with high-quality class groundnut residue (high-nitrogen) had higher DN (5.4 ± 2.6 mg N kg−1) and a lower ratio (6.8 ± 2.6) than those treated with medium-quality (medium-nitrogen) tamarind (3.0 ± 0.6 and 10.7 ± 2.2, respectively). The positive influence of DN on qCO2 (R2 = 0.49 *) in groundnut-treated soil suggested that the high bioavailability of DN reduced CUE due to imbalanced DOC-and-DN stoichiometry. This contradicted earlier published findings on high-nitrogen residues which had balanced DOC-and-DN stoichiometry. The positive influence of the ratio on qCO2 under the tamarind-treated soil (R2 = 0.60 *) indicated that its balanced DOC-and-DN stoichiometry enhanced CUE. High-quality class organic residues can result in either higher or lower CUE than their lower-quality class counterparts depending on whether the resulting DOC-and-DN stoichiometry is balanced or imbalanced.

Development of an alternative low-cost culture medium for a new isolated high-production DHA strain using kitchen wastewater

Docosahexaenoic acid (DHA) is a high-value polyunsaturated fatty acid. The industrial development of microbial DHA was restricted due to high medium cost. Kitchen wastewater was always rich in nitrogen, phosphorous, carbohydrates, which can be used for microorganism growth. This study developed of a primitive low-cost culture medium for a new isolated high-production DHA strain using kitchen wastewater. The results showed that, a total of 66 strains were isolated from mangrove habitats. The highest DHA-producing strain N6 was identified as Aurantiochytrium sp. (GenBank accession number OL455742) with a DHA production of 6.56 g/L. Then the optimization of kitchen wastewater medium was designed by Response Surface Methodology, and 7.14 ∼ 7.23 g/L of DHA production was obtained. At the same time, the lipid content in Aurantiochytrium sp. N6 was as high as 75.68%, which contributed to the increase of DHA production in optimal kitchen wastewater medium. Finally, the substrate cost of kitchen wastewater medium was reduced by 43.95%. Meanwhile, the dissolved organic carbon (DOC), dissolved nitrogen (DN) and dissolved phosphorus (DP) in kitchen wastewater were utilized by Aurantiochytrium sp. N6 more than 90%. This work enriched the competitive candidate DHA-producing microbes, and provided a perceptive idea for both the medium substrate cost reducing and kitchen wastewater recycling.

Runoff nitrogen losses under confluence and diverging drainage systems in the sloped plot scale: A comparative study

The drainage system is a key measure for regulating runoff nutrient losses on sloping farmlands. Confluence and diverging drainage systems are two drainage layouts representing natural water network systems and are widely distributed in sloping farmlands; however, the effects of these drainage systems on runoff nutrient losses in the sloped plots remain unclear. This study investigated the effects of different drainage systems on the characteristics of runoff nitrogen (N) losses in sloped plots using laboratory rainfall simulations. Three treatments, including bare slope (without drainage system, CK), confluence drainage system (T1), and diverging drainage system (T2), were used to compare the changes in concentrations and losses of total nitrogen (TN), dissolved nitrogen (DN), and particulate nitrogen (PN), and the DN:TN ratio in runoff under a combination of 1.8 mm min−1 rainfall intensity and three slope gradients (5°, 10°, and 15°). The results showed that the time to runoff was significantly delayed in T2 compared with that in CK and T1 across all slopes (p < 0.05). Accumulated runoff depth was considerably lower in T1 and T2 than in CK across all slopes (p < 0.05). The TN and PN concentrations in T1 were markedly lower than those in T2 on the 10° and 15° slopes (p < 0.05). The DN concentration in T1 was lowest at the 5° slope (p < 0.05). TN loss in T1 was 14.7–33.9% and 17.9–30.3% lower than those in CK and T2 across all slopes, respectively (p < 0.05). The PN loss in T1 was 56.7% and 53.3% lower than that in T2 on the 10° and 15° slopes, respectively (p < 0.05). DN loss in T1 was 39.3–72.5% lower than that in CK for all slopes (p < 0.05). DN:TN in T2 was lower than that in CK and T1 at the 10° and 15° slopes (p < 0.05). Our results confirm the effectiveness of drainage systems in reducing runoff nutrient losses in a sloped plot and demonstrate that the confluence drainage system is better at reducing N losses in runoff than diverging drainage systems.

Disparities and mechanisms of carbon and nitrogen conversion during food waste composting with different bulking agents

The low C/N ratio, high moisture content, and low porosity of food waste require the addition of bulking agents for adjustment during the composting process. However, the effect and mechanism of different bulking agents on the reduction of carbon and nitrogen losses are unclear. Therefore, this study conducted experiments to evaluate and clarify the differences in carbon and nitrogen transformation between sawdust, rice husk and wheat bran in food waste composting. The results showed that the addition of bulking agents promoted the conversion of carbon and nitrogen into total organic carbon (TOC) and total organic nitrogen (TON) rather than CO2 and NH3. The carbon and nitrogen losses were reduced by 16.00–25.71% and 11.56–29.54%, respectively. Notably, the Sawdust group exhibited the highest carbon retention, whereas the Wheat_bran group demonstrated superior nitrogen retention. The succession of bacterial communities showed that sawdust enhanced the cellulolysis and xylanolysis functions while wheat bran promoted nitrogen fixation. Correlation analysis was further employed to speculate on potential interactions among carbon and nitrogen components. The incorporation of sawdust and rice husk improved humification partly due to the addition of lignocellulose and the accumulation of total dissolved nitrogen (DTN) in the substrate, respectively. In the process of ammonia assimilation, the addition of wheat bran promoted the accumulation of dissolved organic carbon (DOC), contributing to the synthesis of TON to a degree. These findings offer cost-effective strategies for conserving carbon and nitrogen from loss in food waste composting by selecting suitable bulking agents, ultimately producing high-quality fertilizer.

Moderate grazing weakens legacy effects of grazing history on primary productivity and biodiversity in a meadow grassland

Herbivore grazing has long-lasting effects on the structure and functions of grassland ecosystems, even after the grazing pressure has ceased. These lasting effects are known as grazing legacy effects and significantly affect plant productivity and biodiversity, with the magnitude increased with grazing intensity and further led to grassland degradation. Fencing is a common approach used for restoring degraded grasslands by excluding grazing. Moderate grazing representing a controlled level of grazing intensity is believed to be a useful way to restore ecological functions and thus mitigate grazing legacies. However, how fencing and ongoing moderate grazing affect grazing legacy effects remain unclear and rarely tested with field experiments, particularly with different grazing histories. Here, we investigated aboveground net primary productivity (ANPP), species richness, litter, soil organic carbon (SOC), soil dissolved nitrogen (DON) and hyphal length density (HLD) in 2015 (fencing for 1 year) and 2018 (fencing vs moderate grazing for the subsequent 3 years) after 0, 2, 5, 7 and 12 years of free grazing in a meadow steppe to explore how moderate grazing and fencing affect grazing legacy effects with different histories. We observed a decline in ANPP and species richness with increasing grazing histories, and the magnitude of grazing legacy effects increased accordingly. In most grazing histories, moderate grazing had a greater impact than fencing in reducing the grazing legacy effects on ANPP and species richness. However, with a grazing history of 12 years, moderate grazing led to a smaller increase in ANPP compared to fencing. Additionally, the grazing legacy effects on ANPP and species richness weakened with longer fencing duration for excluding grazing. Litter, SOC, DON and HLD exhibited consistent patterns with the dynamics of ANPP and species richness. Our results indicate that grazing legacy effects on ANPP and species richness intensify with prolonged grazing histories, suggesting that grazing history have stronger impacts on grassland ecosystem functions and structure. The study implies that moderate grazing is an effective strategy for restoring degraded grasslands, particularly for species richness and with short-term grazing histories, instead of relying solely on fencing. The changes of soil variables underlie the grazing legacy effects and the interactions with fencing and moderate grazing.

Dissolved organic compounds in shale gas extraction flowback water as principal disturbance factors of soil nitrogen dynamics

Flowback water, a by-product of shale gas extraction, represents an extremely complex industrial wastewater characterized by high organic compounds content and high salinity. The prospect of flowback water entering the soil through various approaches concerns regarding its ecological risk. Nitrogen mineralization (Nmin), a key rate-limiting step in the soil N cycle, might be adversely affected by flowback water. Nonetheless, no previous studies have examined the effects of flowback water on soil Nmin rates, let alone quantified the relative contributions of the major components of flowback water to changes in Nmin rates. Therefore, this study investigated the effects of flowback water and sterile flowback water at two different concentrations on the Nmin rates of three distinct soil types. This study aimed to elucidate the predominant influence of the key constituents within flowback water on the changes in soil Nmin rates. The results showed that soil soluble salt content, dissolved organic carbon (DOC) and dissolved nitrogen (DN) content significantly increased by 8.37 times, 9.5 % and 26.4 %, respectively, in soils contaminated by flowback water. In comparison with the control group, the introduction of flowback water resulted in a significant 25.9 % reduction in Nmin rate in sandy soils. Conversely, in clay and loam soils, there was a significant increase in Nmin rates by 44.9 % and 131.8 % respectively. Throughout the incubation period, leucine-aminopeptidase activity exhibited irregular fluctuations. Analysis of microbial communities demonstrated that flowback water only significant impacted soil rare microbial taxa, inducing a significant increase in alpha diversity for sandy, clay, and loamy soils by −16.9 %, 10.12 %, and 1.63 %, respectively. Linear regression and random forest analyses indicated that alterations in soil DOC:DN ratio and salt content were responsible for changes in soil Nmin rates within flowback water-contaminated soils. In contrast, only salt content significantly contributed to shifts in alpha diversity among soil rare microbial taxa. Structural equation modeling highlighted that the total effect of dissolved organic compounds (DOC and DN, λ = 0.64) from flowback water was greater than the total effect of salinity (λ = 0.24) on soil Nmin rates. In conclusion, our findings imply that dissolved organic compounds within flowback water play pivotal roles in determining soil Nmin rates. To the best of our knowledge, this is the first study to reveal the effects of major components in the flowback water on soil N mineralization rates.

How to control nitrogen and phosphorus loss during runoff process? – A case study at Fushi Reservoir in Anji County (China)

Water pollution is becoming a global issue, and process controls are the key steps for controlling non-point source pollution (NPSP). Runoff ditches collect rainfall and often act as channels for nitrogen (N) and phosphorus (P) pollutants to enter water source areas such as the reservoir, which are important parts of process controls. Therefore, performing in situ analyses to clarify the mechanism of process control are vital for reducing the migration of N and P pollutants into reservoirs. In view of this, the Fushi Reservoir in Taihu lake basin was taken as the test plot and the characteristics of process controls were approached through field investigations to determine the impact of runoff ditch landscape factors on water quality. The results indicated that various landscape factors had different effects on the N and P concentrations in the runoff ditch. Some landscapes that were influenced by human activities, including residential and white tea gardens, could increase N but had relatively little impact on P levels in the runoff ditch. Compared with the white tea (WT3), forested wetlands (FW2) decreased the average total nitrogen (TN) (-29.53%), average dissolved nitrogen (DN) (-40.29%), and average nitrate-nitrogen (-45.32%) in the runoff ditch. Obviously, the novel research suggests constructing rationally landscape factors for runoff ditches is helpful to mitigate NPSP in catchment areas of drinking water and to improve water quality. It is expected that the initial research can push up landscape planning of runoff ditches and promote the improvement of water quality in water source areas.

Modulation effects of different dissolved nitrogen compositions on the phytoplankton community structure based on combined field experiments and the NbPD model in Jiaozhou Bay

The dissolved nitrogen (DN) regime in Jiaozhou Bay (JB) has shifted recently, and the phytoplankton community structure showed decreased diatom and increased dinoflagellate concentrations. This study combined field microcosm experiments, the Nutrients–bi-Phytoplankton–Detritus (NbPD) biogeochemical model, and statistical analysis, and obtained some results. The growth/death of dominant phytoplankton populations were comprehensively determined by the processes of nutrient uptake, excretion and decomposition of phytoplankton in JB. Kinetic parameters indicated that Skeletonema costatum preferentially absorbed NH4-N and NO3-N, while Protoperidinium pellucidum preferentially absorbed domestic-derived dissolved organic nitrogen. Moreover, S. costatum, as R-strategists, needed a faster uptake rate and slower excretion rate to achieve high growth efficiency but with faster decomposition, while P. pellucidum, as K-strategists, could maintain stability with a more active metabolism. Overall, this study discussed the key factors modulating the diatom dinoflagellate community structure changes in JB, providing a scientific basis for ecosystem-based management.

Distributions of Nutrients in Relation to Phytoplankton Community Heterogeneity in the Makassar Strait, Indonesia

The Monitoring of Indonesia Throughflow (MITF) with scientific cruise across the Indonesian waters that are connecting the islands of Kalimantan and Sulawesi or strait of Makassar. This cruise carried out in August 2015 in an area between 2° and 4° South and 118° and 119° East. This cruise aims to characterize the concentration of nutrients and abundance of phytoplankton groups as well as explain their distribution based on the environmental conditions. Nutrient concentrations consisting of Inorganic Dissolved Nitrogen (DIN), Inorganic Dissolved Phosphate (DIP) and Inorganic Dissolved Silicate (DSi) were measured at seven depth layers, i.e. 10, 30-60, 100-150, 300, 500, 750 and 1000 meters. The study showed that in the transitional season (August), the upper layer of study sites were characterized by salinity between 34.05 and 34.76 PSU, temperature between 26.85 and 29.88 °C, and relatively high nutrient concentration, i.e. DIN = 8.91 - 36.74 μM, DIP = 0.02 - 0.16 μM, and DSi= 31.88 - 249.49 μM. The phytoplankton community groups found were Cyanophyta, Bacillariophyta (Diatom), and Dinophyta, where the highest abundance was Cynophyta for the genus Trichodesmium sp. The composition of phytoplankton community in the surface layer is strongly influenced by sea surface temperature, salinity and nutrient concentration. We found that nutrient utilization in the surface layer is relatively high.

Determining nitrogen fate by hydrological pathways and impact on carbonate weathering in an agricultural karst watershed

Identifying the nitrogen (N) fate is complicated and a great challenge in karst watersheds because of the co-existence of natural pools and anthropogenic sources. The objective of the study was to use stable isotopic composition of dual-isotope (δ15NNitrate and δ18ONitrate) and LOADEST model approaches to trace N sources, pathways in karst watershed. The study was conducted in the Houzhai watershed, which is a typical agricultural karst watershed from July 2016 to August 2018, to reveal the N fate and the coupled carbon(C)–N processes occurring in the riverine-watershed with agricultural activities. We found that the wet deposition of total nitrogen (TN) flux was 33.50 kg hm−2·a−1 and dissolved nitrogen (DN) flux was 21.66 kg hm−2·a−1. The DN runoff loss was 2.10 × 105 kg·a−1 and the loss of DN during the wet season accounted for 95.4% over a year. In the wet season, NO3−-N daily efflux was 977.62 ± 516.66 kg ha−1·day−1and 248.77 ± 57.83 kg ha−1·day−1 in the dry season. The NH4+-N efflux was 29.17 ± 10.50 kg ha−1·day−1 and 4.42 ± 3.07 kg ha−1·day−1 in the wet and dry seasons, respectively. The main form output load of N was NO3−-N which was more than 30 times as much as NH4+-N output loss. The NO3−-N caused by rainfall contributed 11.82%–53.61% to the export load. Nitrate from soil contributed over 94% of the N to Houzhai river caused by N leaching. In addition, manure and farmland soil were the main sources of groundwater in the Houzhai watersheds, the contribution rates were 25.9% and 22.5%. The chemical N fertilizers affected carbonate weathering strongly, and the HCO3− flux caused by nitrification due to N fertilizers application in soil accounted for 23.5% of the entire watershed. This study suggested that carbonate weathering may be influenced by nitrogen nitrification in the karst watershed.

Rainfall partitioning and its influence on dissolved carbon and nitrogen fluxes through plant-soil system of a xerophytic shrub in northern China

Dissolved carbon (DC) and dissolved nitrogen (DN) driven by rainfall partitioning are critical components of carbon and nitrogen cycles in terrestrial ecosystems, especially in vegetation restoration areas. However, the variations of DC and DN fluxes in the input (throughfall and stemflow) and output (infiltration and surface runoff) water pathways of shrub ecosystem in drylands were poorly understood. In this study, the rainfall partitioning, dissolved organic and inorganic carbon (DOC and DIC) and DN fluxes in 52 rainfall events affecting a xerophytic shrub ecosystem on the heavily eroded Chinese Loess Plateau were measured over the 2019–2021 rainy seasons. The contributions of plants and topsoil (0–5 cm) to the variations in DC and DN fluxes along water pathways were quantified. We found that the DC and DN fluxes in throughfall accounted for more than 80% of net input flux, and the topsoil infiltration of DC and DN fluxes into soil layer below 5 cm depth were the most important output flux (contribution more than 98%). The mean event plant-derived DC and DN fluxes were 52.2 and 5.3 mg m−2, respectively, accounting for 45.4% and 12.2% of net DC and DN input fluxes (115.2 and 43 mg m−2). The soil-buffered DC and DN fluxes (455.6 and 29.5 mg m−2) were 3.95 and 0.69 times greater than the net input fluxes, respectively. The DC and DN fluxes in infiltration had the best correlation and increased most quickly with rainfall depth in the four different water pathways. Furthermore, the soil carbon content in 0–50 cm depth and soil nitrogen content in 0–10 cm depth in shrub-covered land was significantly higher than those in bare land (p < 0.05). This study highlights the important roles of rainfall partitioning in plant-sourced and soil-buffered DC and DN fluxes, which should be considered in soil carbon and nitrogen accumulation in drylands.

Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

PurposeThis study aimed at valorizing solid food waste containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA).Methods Aurantiochytrium sp. L3W that produces DHA and EPA was cultivated on eight types of solid food waste: sake lees (SL), crown daisy, Japanese mustard spinach (JMS), soy sauce residue, lemon peel (LP), orange peel, grape skin, and Hiroshimana old pickle (HOP). The biomass mixture of the remaining food waste and strain L3W was analyzed for DHA and EPA. To characterize the types of food waste, the leachability of dissolved organic carbon (DOC) and dissolved nitrogen (DN) was compared.ResultsThe strain L3W grew on both pasteurized and unsterilized food waste such as SL and JMS. Elution of DOC and DN from the food waste might be a factor affecting the growth of strain L3W. However, the strain L3W might utilize solid-state organic compounds in JMS. Despite the unsterile conditions, the biomass mixture of SL contained both DHA and EPA, whereas DHA was found in the biomass mixtures of JMS, LP and HOP, thereby confirming the valorization of these types of solid food waste. Unsterile mass cultivation of the strain L3W using SL and HOP in a 200 L tank also produced a biomass mixture containing 12.6 mg-DHA/g and 0.217 mg-EPA/g. These DHA and EPA contents were 1500-times and 37-times higher, respectively, than that in commercial poultry feed, indicating that the biomass mixtures could be used as an additive in poultry feed.Graphical Abstract

Effect of straw biochar application on soil carbon, greenhouse gas emissions and nitrogen leaching: A vegetable crop rotation field experiment

AbstractIntensive vegetable crop systems are rapidly developing, with consequences for greenhouse gas (GHGs) emissions, nitrogen leaching and soil carbon. We undertook a field trial to explore the effect of biochar application (0, 10, 20 and 40 t ha−1) on these factors in lettuce, water spinach and ice plant rotation. Our results show that the 20 and 40 t ha−1 soil treatments resulted in the SOC content being 26.3% and 29.8% higher than the control (0 t ha−1), respectively, with significant differences among all treatments (p &lt; .05). Biochar application caused N2O emissions to decrease during the lettuce and water spinach seasons, by 1.5%–33.6% and 12.4%–40.5%, respectively, compared the control, with the 20 t ha−1 application rate resulting in the lowest N2O emissions. Biochar also decreased the dissolved nitrogen (DN) concentration in leachate by 9.8%–36.2%, following a 7.3%–19.9% reduction in dissolved nitrogen in the soil. Similarly, biochar decreased the nitrate (NO3−) concentrations in leachate by 3.9%–30.2%, following a 3.8%–16.7% reduction in the soil nitrate level. Overall, straw biochar applied at rate of 20 t ha−1 produced the lowest N2O emissions and N leaching, while, increasing soil carbon.

Features of the Seasonal Dynamics of Dissolved Nitrogen Forms in the Lower Stream of the Severnaya Dvina River

Features of the Seasonal Dynamics of Dissolved Nitrogen Forms in the Lower Stream of the Severnaya Dvina River

The Composition of Dissolved Organic Matter in Arable Lands: Does Soil Management Practice Matter?

Dissolved organic matter (DOM) is a key soil quality property, indicative of the organic matter stored in the soil, which may also be a function of temporal variation. This study examines whether DOM is a robust property of the soil, controlling fertility, or if it may change with time. Altogether eight sets of soil samples were collected in 2018 and 2019 from the cultivated topsoil (0–10 cm) of cropland and from a nearby grassland near Martonvásár, Hungary. The study sites were characterized by Chernozem soil and were part of a long-term experimental project comparing the effects of manure application and fertilization to the control under maize and wheat monocultures. DOM was extracted from the samples with distilled water. The dissolved organic carbon (DOC), total dissolved nitrogen (DN), biological index (BIX), fluorescence index (FI), humification index (HIX), carbon nitrogen (C/N) ratio and specific ultraviolet absorbance at 254 nm (SUVA254) index were studied in the arable soils, and the results showed that all the DOM samples were humified, suggesting relevant microbiological contributions to the decomposition of OM and its conversion into more complex molecules (FI = 1.2–1.5, BIX = ~0.5, and HIX = ~0.9). Temporal variations were detected only for the permanent grassland where higher DOM concentration was found in spring. This increased DOM content mainly originated from humified, solid phase associated, recalcitrant OM. In contrast, there were no differences among fertilization treatments and sampling dates under cropfield conditions. Moreover, climatic conditions were not proven as a general ruler of DOM properties. Therefore, momentary DOM alone is not necessarily the direct property of soil organic matter under cropfield conditions. The application of this measure needs further details of sampling conditions to achieve adequate comparability.

Dynamics of soluble soil organic matter in Mediterranean maize-based forage system under organic and mineral fertilization

In Nitrate Vulnerable Zones (NVZ), the impact of animal effluent management on soil fertility is essential. A three-year study was carried out in a NVZ (Arborea, Central-western Sardinia, Italy), aiming to analyze the effect of different fertilization systems on soluble and total soil organic matter (SOM) within a silage maize-hay crop cropping system on sandy soil (Oxyaquic Xeropsamment). Four types of fertilization systems were compared: (i) cattle manure; (ii) cattle slurry; (iii) mineral; (iv) slurry + mineral. The water-extractable C and N content (WEC and WEN) from the topsoil (0–20 cm layer, the Ap1 horizon), the dissolved carbon (DC) and the dissolved nitrogen (DN) of the soil solution collected by disk lysimeters placed along the soil profiles, and the C content at the beginning and at the end of the experiment were determined.The seasonal dynamics of WEC in the topsoil seemed mainly associated with the timing of the fertilizer distribution. In the topsoil, WEC was larger under slurry than under mineral treatment. The DC was rather low in the deeper soil layers and did not show any variation due to the fluctuations of fertilizer distribution that were seemingly influenced by the groundwater table. The fertilization systems influenced the soil C inputs and, in turn, the SOM content. Manure promoted a higher C stock in the deeper soil layers among the organic fertilizers. However, the hay crop added with manure and slurry showed lower crop residues and, thus, C input than that added with mineral fertilizer. This result suggests that the management of these organic fertilizers should carefully consider their impact on the overall productivity of the studied cropping system in Mediterranean NVZ to avoid a potential limitation for soil C accumulation in the long term.

Modelling the removal of nitrogen and sediment by a constructed wetland system in north Queensland, Australia

This study of a multi-component 2.5 ha constructed wetland system near Mackay, north Queensland was established to determine the efficacy of these types of wetlands for improving water quality entering the Great Barrier Reef lagoon. To do this a water balance model was developed using water depth measurements made in the wetland inlet basin, between 25 December 2018 and 30 September 2020. The water balance model allowed daily values of run-in and drainage to be calculated, which were combined with a nitrogen balance model to derive a value for the amount of total nitrogen (TN) removed by the wetland of 83 kg ha−1 year−1, or 37% of the nitrogen input to the wetland. The nitrogen balance model used a mean inflow TN concentration of 5060 (± 1734) μg L−1 derived from TN concentration measurements (automatic and grab) made during four high flow events. The largest losses of nitrogen where via drainage with 49% leaving as outflow and 14% as groundwater seepage. Outflow carried 38% of the sediment and particulate nitrogen (PN) out of the wetland, which means that 62% settled to the bottom of the wetland. Dissolved nitrogen (DN) removal in Bakers Creek is mainly (89%) due to the relatively long residence time of water in its biodiversity pond component, the largest and deepest part of this wetland system which always contained water, even during the dry season. This illustrates the value of large, permanent deep pools in a wetland system, whether they are constructed or natural. Wetlands constructed downstream of areas generating sediments and nutrients should therefore be able to improve the quality of water entering the Great Barrier Reef lagoon. However, to do this they need to retain water for significant amounts of time, which they may do in comparatively low rainfall areas. In higher rainfall areas open, flow through wetlands may not be able to do this, so wetlands constructed in these regions need to be designed to retain water in order to have significant N removal capacity. Proper monitoring of future constructed wetlands is vital in order to derive robust design criteria for systems that can remove significant nitrogen from agricultural run-off water.