Displacement Device Research Articles

Pore-Scale Formation Characteristics of Impermeable Frozen Walls for Shallow Groundwater Contamination Remediation

Impermeability and water blocking are crucial for remediating shallow groundwater contamination. Traditional methods often employ curtain-grouting technology to create impermeable layers. However, cement slurry curing is irreversible, leading to permanent closure of underground aquifers and secondary pollution. This study employs an innovative approach by fabricating cylindrical models that simulate actual strata and utilizing a high-temperature and high-pressure displacement device. It systematically analyzes the variations in soil pore structure, distribution, porosity, and permeability under different temperatures, pressures, and freezing durations. The microscopic characteristics of the freezing process in water-bearing soils were studied. Results demonstrate that longer freezing time improves the effectiveness of soil freezing, reaching complete freezing at temperatures as low as −4 °C for samples with low water content. For water-saturated samples, freezing below −6 °C results in nearly zero porosity. Increased pressure at a certain freezing temperature significantly reduces permeability. When freezing temperature falls below −4 °C, water permeability in saturated samples after freezing reaches near-zero levels, while unsaturated samples experience complete freezing. These findings provide a theoretical foundation for constructing freezing curtains in remediating shallow groundwater pollution.

Interplay of Size, Deformability, and Device Layout on Cell Transport in Microfluidics.

Microfluidics have been widely used for cell sorting and capture. In this work, numerical simulations of cell transport in microfluidic devices were studied considering cell sizes, deformability, and five different device designs. 
Among these five designs, deterministic lateral displacement device (DLD) and hyperuniform device (HU) performed better in promoting cell-micropost collision due to the continuously shifted micropost positions as compared with regular grid, staggered, and hexagonal layout designs. However, the grid and the hexagonal layouts showed best in differentiating cells by their size dependent velocity due to the size exclusion effect for cell transport in clear and straight paths in the flow direction. A systematic study of the velocity differentiation under different dimensionless groups was performed showing that the velocity difference is dominated by the micropost separation distance perpendicular to the direction of flow. Microfluidic experiments also confirmed the velocity differentiation results. The study can provide guiding principles for microfluidic design.

A High-Aspect-Ratio Deterministic Lateral Displacement Array for High-Throughput Fractionation.

Future industrial applications of microparticle fractionation with deterministic lateral displacement (DLD) devices are hindered by exceedingly low throughput rates. To enable the necessary high-volume flows, high flow velocities as well as high aspect ratios in DLD devices have to be investigated. However, no experimental studies have yet been conducted on the fractionation of bi-disperse suspensions containing particles below 10 µm with DLD at a Reynolds number (Re) above 60. Furthermore, devices with an aspect ratio of more than 4:1, which require advanced microfabrication, are not known in the DLD literature. Therefore, we developed a suitable process with deep reactive ion etching of silicon and anodic bonding of a glass lid to create pressure-resistant arrays. With a depth of 120 µm and a gap of 23 µm between posts, a high aspect ratio of 6:1 was realized, and devices were investigated using simulations and fractionation experiments. With the two-segmented array of 3° and 7° row shifts, critical diameters of 8 µm and 12 µm were calculated for low Re conditions, but it was already known that vortices behind the posts can shift these values to lower critical diameters. Suspensions with polystyrene particles in different combinations were injected with an overall flow rate of up to 15 mL/min, corresponding to Re values of up to 90. Suspensions containing particle combinations of 2 µm with 10 µm as well as 5 µm with 10 µm were successfully fractionated, even at the highest flow rate. Under these conditions, a slight widening of the displacement position was observed, but there was no further reduction in the critical size as it was for Re = 60. With an unprecedented fractionation throughput of nearly 1 L per hour, entirely new applications are being developed for chemical, pharmaceutical, and recycling technologies.

Contact Characteristics and Characterization Methods of CO2 and Crude Oil in Visual Porous Medium

In this article, the vertical static variation characteristics of CO2 and crude oil in porous medium under different experimental pressures are studied by using the CO2 miscible visual displacement device jointly developed. The process of change at the CO2 and crude oil interface is characterized by relative height and dissolution expansion rate. Experimental results demonstrate that with the increase of pressure, CO2 gradually reaches a supercritical state with strong extraction and mass transfer capacity, and CO2‐crude oil exhibits different contact forms under different pressures. Under the same pressure, there are also significant differences in contact forms between visual tube and porous medium. The existence of porous medium weakens the mass transfer and diffusion abilities between CO2 and crude oil and reduces the extraction of light components by CO2. In porous medium, the relative height of crude oil is a power function of time, which is 1/5 ≈ 3/10 of that in visual tube. And the dissolution expansion rate of CO2 and crude oil exhibits a negative logarithmic correlation with time, which is 1/4 ≈ 2/5 of that in visual tube. The impact of porous media on relative height and dissolution expansion rate of crude oil becomes more pronounced as the pressure decreases.

Focal length measurement based on vortex beam interference

This paper proposes a new method to measure the focal lengths of both convex and concave lenses based on vortex beam interference. Theoretical analysis illustrates that the maximum intensity distribution of the interferogram is in accordance with the Fermat's spiral, and the spiral coefficient is proportional to the difference between the focal length of the test lens and the distance from the test lens to the camera. In order to calculate the precise focal length, we separately block the light paths to obtain the reference and test beam spots, and get the ratio of these two spot sizes. Then, the ratio and the spiral coefficient are used to determine the focal length. For a biconvex lens with a focal length of 200 mm and a biconcave lens with a focal length of −100 mm, the relative measurement errors are 0.0892 % and 0.0800 %, respectively. This research provides a novel and convenient method for measuring the focal length, and no-sophisticated displacement devices and moving the lens to be measured are required in the measurement.

Dependence of the Values of Inter-Component Phase Relations of the Harmonic Components of Vibration on the Displacement of Misaligned Shafts

The analytical dependence of the phases and values of intercomponent phase relations of the harmonic components of vibration displacement of misaligned shafts on their displacement has been presented in the article. Harmonic vibrations of the shafts were modeled using a Lagrangian connecting vibration displacement and forces acting in the system. For ease of derivation, the Lagrangian is rewritten in complex form. The resulting algebraic equations connect the complex amplitudes of vibration components with the amplitudes of periodic forces at the same frequencies and the parameters of the mechanical system (stiffness, damping). The amplitudes of periodic forces are related to the magnitude of the shafts angular and parallel misalignment according to the accepted model. Calculation of intercomponent phase relations values is used to get rid of the random initial phase shift caused by the choice of the timing reference. The magnitude of the effect produced by the misalignment of the shafts on the amplitudes and values of the intercomponent phase relations of the signal components is estimated. The obtained resultcan be used to construct devices for estimation shaft displacements and devices for assessing the condition of equipment based on the analysis of vibration signals.ls.

Rapid cell isolation in breastmilk in a non-clinical setting by a deterministic lateral displacement device and selective water and fat absorption.

Breastmilk is a reliable source of biomarker-containing, sloughed breast cells that have the potential to give valuable health insights to new mothers. Furthermore, known DNA-based markers for pregnancy-associated breast cancer are chemically stable and can be safely stored on a commercially available FTA® Elute Micro (EM) card, which can subsequently be mailed to a testing facility for the cost of a stamp. In theory, this archiving process can be performed by nonprofessionals in very low-resource settings as it simply requires placing a drop of breastmilk on an EM card. Although this level of convenience is paramount for new mothers, the low cell density of breastmilk complicates archiving on an EM card as such commercial products and associated protocols were designed for high-cell density physiological fluids such as blood. In this study, we present the use of a deterministic lateral displacement (DLD) device combined with porous superabsorbent polymers and hydrophobic sponges to achieve simple and low-cost cell enrichment in breastmilk. As the critical separation diameter in a DLD device is more heavily dependent on lithographically controlled pillar layout than fluid or flow properties, our use of DLD microfluidics allowed for the accommodation of both varying viscosities in human breastmilk samples and a varying pressure of actuation resulting from manual, syringe-driven operation. We demonstrate successful cell enrichment (>11×) and a corresponding increase in the DNA concentration of EM card elutions among breastmilk samples processed with our hybrid microfluidic system. As our device achieves sufficiently high cell enrichment in breastmilk samples while only requiring the user to push a syringe for 4 min with reasonable effort, we believe that it has high potential to expand EM card DNA archiving for diagnostic applications with low-cell density physiological fluids and in low-resource settings.

Unraveling the motion and deformation characteristics of red blood cells in a deterministic lateral displacement device

Deterministic Lateral Displacement (DLD) device has gained widespread recognition and trusted for filtering blood cells. However, there remains a crucial need to explore the complex interplay between deformable cells and flow within the DLD device to improve its design. This paper presents an approach utilizing a mesoscopic cell-level numerical model based on dissipative particle dynamics to effectively capture this complex phenomenon. To establish the model’s credibility, a series of numerical simulations were conducted and the numerical results were validated with nominal experimental data from the literature. These include single cell stretching experiment, comparisons of the morphological characteristics of cells in DLD, and comparison the specific row-shift fraction of DLD required to initiate the zigzag mode. Additionally, we investigate the effect of cell rigidity, which serves as an indicator of cell health, on average flow velocity, trajectory, and asphericity. Moreover, we extend the existing theory of predicting zigzag mode for solid spherical particles to encompass the behavior of red blood cells. To achieve this, we introduce a new concept of effective diameter and demonstrate its applicability in providing highly accurate predictions across a wide range of conditions.

Appraisal of the Accuracy and Reliability of Cone-Beam Computed Tomography and Three-Dimensional Printing for Volumetric Mandibular Condyle Measurements of a Human Condyle.

Background This study aims to evaluate the accuracy of volumetric measurements of three-dimensional (3D)-printed human condyles from cone-beam computed tomography (CBCT) in comparison to physical condyles using a water displacement test. Methodology A sample of 22 dry condyles was separated from the mandibular body by disc, mounted on a base made of casting wax, and scanned using the SCANORA (Scanora 3DX, Soredex, Finland) CBCT scanner. Subsequently, the projection data were reconstructed with the machine-dedicated OnDemand 3D (Cybermed Co., Seoul, Korea). The Standard Tessellation Language file was prepared for 3D printing using chitubox slicing software v1.9.1. Frozen water-washable gray resin was used for 3D printing. All condyles were printed using the same parameters and the same resin. The volumetric measurements were then performed using a customized modified pycnometer based on water volume and weight displacement. Volumetric measures were performed for both the physical human condyles and the 3D-printed replicas and the measurements were then compared. Results The volume of dry condyles using the water displacement method showed an average (±SD) of 1.925 ± 0.40 cm3. However, the volume of 3D-printed replicas using the water displacement method showed an average (±SD) of 2.109 ± 0.40 cm3. The differences in measurements were insignificant (p > 0.05), as revealed by an independent t-test. Conclusions Highly precise, accurate, and reliable CBCT for volumetric mandibular condyle was applied for measurements of a human condyle and 3D-printed replica. The modified pycnometer for volumetric measurements presented an excellent volumetric measure based on a simple water displacement device. The tested modified pycnometer can be applied in volumetric measurements in both 3D-printed and mandibular condyle.For best accuracy, the highest scanning resolution possible should be used.As it directly handles irregularly shaped solid objects in a non-destructive manner with a high level of precision and reliability, this 3D scanning approach may be seen as a superior alternative to the current measurement methods.

Mesoscopic simulation of multi-scheme particle separation in deterministic lateral displacement devices using two-piece hybrid pillars

Pillar shape exploration in deterministic lateral displacement (DLD) technique holds great promise for developing high-performance microfluidic devices with versatile sorting schemes. A recent innovative design using filter-like micropillars was proposed to improve cell separation, but its significance might be greatly underestimated due to an inaccurate understanding of the underlying mechanism. In this study, we employ mesoscopic hydrodynamic simulations to explore the movement and separation of rigid spherical particles in DLD arrays using various two-piece hybrid (TPH) pillars, where each pillar consists of two individual pieces separated by a tunable inter-piece channel. In comparison with the conventional one-piece pillars, the back piece of TPH-pillars is found to hierarchically tailor the flow profile of the front piece on the basis of the row shift fraction and the inter-piece channel width, resulting in unique tunable multi-scheme separation at low, intermediate, and high row shift fractions, respectively. At the intermediate regime, in particular, the first flow lane that determines the critical separation size could be physically fenced out by the inter-piece channel, and a delicate coupling of hydrodynamic filtration and DLD has been revealed to induce a constant critical size in the whole regime. This work theoretically demonstrates the feasibility and significance of TPH-pillars, which may open up a new direction of the geometry design by exploiting rich multi-piece hybrid structures to expand the versatility of the DLD technique.

Stiffness influence on particle separation in polydimethylsiloxane-based deterministic lateral displacement devices

Polydimethylsiloxane (PDMS) is a popular material to rapidly manufacture microfluidic deterministic lateral displacement (DLD) devices for particle separation. However, manufacturing and operation challenges are encountered with decreasing device dimensions required to separate submicron particles. The smaller dimensions, notably, cause high hydraulic resistance, resulting in significant pressure even at relatively low throughputs. This high pressure can lead to PDMS deformation, which, in turn, influences the device performance. These effects may often be overlooked in the design and operation of devices but provide a systematic source of error and inaccuracies. This study focuses in detail on these effects and investigates pillar deformation in detail. Subsequently, we discuss a potential solution to this deformation using thermal annealing to stiffen the PDMS. We evaluate the influence of stiffness on the separation performance at elevated sample flow rates with submicron particles (0.45 and 0.97 µm diameter). An excellent separation performance at high throughput is successfully maintained in stiffer PDMS-based DLD devices, while the conventional devices showed decreased separation performance. However, the increased propensity for delamination constrains the maximal applicable throughput in stiffer devices. PDMS deformation measurements and numerical simulations are combined to derive an iterative model for calculating pressure distribution and PDMS deformation. Finally, the observed separation characteristics and encountered throughput constraints are explained with the iterative model. The results in this study underline the importance of considering pressure-induced effects for PDMS-based DLD devices, provide a potential mitigation of this effect, and introduce an approach for estimating pressure-induced deformation.

Nitrogen Foam Alternating Flooding Experiment in The Offshore Strong Bottom Water Sandstone Reservoir

The CFD oilfield is located in the Bohai Sea area and is a typical offshore sandstone reservoir with strong bottom water. The characteristic of the rapid increase in water content is that it quickly enters a highly high water content period, and there currently needed to be more potential tapping measures affected by offshore production facilities. The development mode should be changed to explore the development effect of nitrogen foam flooding. This study conducted two experiments. The first experiment is a gas injection expansion experiment, which studied the changes in bubble point pressure, fluid volume, density, and viscosity under different gas injection conditions. The second experiment used a high-temperature and high-pressure oil displacement device and a long core for oil displacement experiments. Two typical sand bodies were selected for the N2 foam oil displacement experiment. The experimental results show that nitrogen injection has little effect on the properties of reservoir crude oil, with a viscosity reduction effect of 12.4% for heavy oil and 20.0% for thin oil. The viscosity of crude oil significantly impacts the selection of alternative media. The channel was prone to blockage and low oil displacement efficiency when injecting a single gas medium into a heavy oil system. The displacement efficiency of thin oil nitrogen foam is 73.28%. The displacement efficiency of nitrogen foam flooding for heavy oil is 43.09%. This study has a guiding significance for developing development plans for offshore sandstone reservoirs.

Safety of an esophageal deviator for atrial fibrillation catheter ablation.

Esophageal thermal injury is a complication of atrial fibrillation (AF) ablation, and it can be avoided by esophageal deviation during left atrial posterior wall radiofrequency catheter ablation. This study aimed to evaluate the safety of a nitinol-based mechanical esophageal displacement device (MEDD) and its performance. This preclinical safety study was conducted on 20 pigs, with 10 undergoing radiofrequency AF ablation using the MEDD and 10 serving as a control group under anticoagulation but without radiofrequency application. Esophageal traumatic injuries were classified from 0 to 4 and were grouped as absent (grade 0), minor (grade 1 or 2), moderate (grade 3), or major risk lesions (grade 4) by anatomopathological study. Grades 1 and 2 were considered acceptable. Fluoroscopy was used to measure displacement. Five (25%) pigs developed traumatic lesions, 4 with grade 1 and 1 with grade 2 (2-mm superficial ulcer). There was no difference in lesion occurrence between the radiofrequency and control groups (30% and 20%, respectively; P = .43). Under rightward displacement, the right edge moved 23.9 (interquartile range [IQR] 21.3-26.3) mm and the left edge moved 16.3 (IQR 13.8-18.4) mm (P < .001) from baseline. Under leftward displacement, the right edge moved 13.5 (IQR 10.9-15.3) mm and the left edge moved 16.5 (IQR 12.3-18.5) mm (P = .07). A perforation to the pharyngeal diverticulum occurred in 1 pig, related to an accidental extubation. In pigs, the MEDD demonstrated safety in relation to esophageal tissue, and successful deviation. Esophageal traumatic injuries were acceptable, but improper manipulation led to pharyngeal lesion.

Metal-mediated DNA strand displacement and molecular device operations based on base-pair switching of 5-hydroxyuracil nucleobases

Rational design of self-assembled DNA nanostructures has become one of the fastest-growing research areas in molecular science. Particular attention is focused on the development of dynamic DNA nanodevices whose configuration and function are regulated by specific chemical inputs. Herein, we demonstrate the concept of metal-mediated base-pair switching to induce inter- and intramolecular DNA strand displacement in a metal-responsive manner. The 5-hydroxyuracil (UOH) nucleobase is employed as a metal-responsive unit, forming both a hydrogen-bonded UOH–A base pair and a metal-mediated UOH–GdIII–UOH base pair. Metal-mediated strand displacement reactions are demonstrated under isothermal conditions based on the base-pair switching between UOH–A and UOH–GdIII–UOH. Furthermore, metal-responsive DNA tweezers and allosteric DNAzymes are developed as typical models for DNA nanodevices simply by incorporating UOH bases into the sequence. The metal-mediated base-pair switching will become a versatile strategy for constructing stimuli-responsive DNA nanostructures, expanding the scope of dynamic DNA nanotechnology.

Eliminating the need for manual segmentation to determine size and volume from MRI. A proof of concept on segmenting the lateral ventricles.

Manual segmentation, which is tedious, time-consuming, and operator-dependent, is currently used as the gold standard to validate automatic and semiautomatic methods that quantify geometries from 2D and 3D MR images. This study examines the accuracy of manual segmentation and generalizes a strategy to eliminate its use. Trained individuals manually measured MR lateral ventricles images of normal and hydrocephalus infants from 1 month to 9.5 years of age. We created 3D-printed models of the lateral ventricles from the MRI studies and accurately estimated their volume by water displacement. MRI phantoms were made from the 3D models and images obtained. Using a previously developed artificial intelligence (AI) algorithm that employs four features extracted from the images, we estimated the ventricular volume of the phantom images. The algorithm was certified when discrepancies between the volumes-gold standards-yielded by the water displacement device and those measured by the automation were smaller than 2%. Then, we compared volumes after manual segmentation with those obtained with the certified automation. As determined by manual segmentation, lateral ventricular volume yielded an inter and intra-operator variation up to 50% and 48%, respectively, while manually segmenting saggital images generated errors up to 71%. These errors were determined by direct comparisons with the volumes yielded by the certified automation. The errors induced by manual segmentation are large enough to adversely affect decisions that may lead to less-than-optimal treatment; therefore, we suggest avoiding manual segmentation whenever possible.

Can a colonial flag become a banner for democracy? The Case of the Dragon and Lion flag and the 2019 Hong Kong protests

Why did Hong Kong protestors choose a symbol of former oppression – the old colonial flag – as a banner for their fight for democracy, rights and autonomy in 2019? We propose to answer this puzzle by studying the colonial-era flag as a displacement device. The waving of the colonial-era flag is shown to induce non-linear temporal and extraterritorial displacements, as well as contradictory interpretations of Hong Kong’s core values, national sovereignty and cultural identity. The flag’s displacements are amplified against the contested colonial history of the former British enclave. Conceptually, this pragmatic definition of the flag moves beyond approaches that study flags as representations of a structure of symbolic meaning. The flag is neither an unimportant prop nor is it a free-floating signifier; its materiality elicits significant political effects. Methodologically, this translates into an exploration of the flag’s second-order agency. The old colonial-era Hong Kong flag, in combination with discourse and institutional arrangements, is shown to be integral to contentious politics. The flag and its displacements shed new light on a city uneasy with its past, dissatisfied with its present and uncertain about its future.

An alarm device for mechanical compression device displacement at femoral artery puncture sites

Objective To develop an alarm device for the mechanical compression device displacement (MCD), and further evaluate its effectiveness in clinical use. Material and methods The alarm device is mainly composed of buzzer, indicator light, magnetic sheet. This is a prospective randomized and controlled study. Four hundred patients who met the inclusion/exclusion criteria were included and randomly assigned to two groups (MCD group vs alarm + MCD group). The primary outcome measures were the sensitivity and specificity of the alarm device to detect MCD displacement, time to hemostasis (TTH), time to ambulation (TTA), time to hospital discharge (TTHD), hospital costs (HC), complication rates, and patient satisfaction. Results The sensitivity and specificity of the alarm device in detecting MCD displacement were 94.44% and 88.46%, respectively. The study group achieved shorter TTH (p = .034), shorter TTA (p = .021), lower complication rates (p = .025), and better patients’ satisfaction (p < .001) compared to the control group. However, no significant difference was observed in TTHD (p = .361) and HC (p = .583). Conclusion The alarm device is highly sensitive in detecting MCD displacement, while achieving better clinical outcomes compared with artificial monitoring.

Blood Cell Separation Using Polypropylene-Based Microfluidic Devices Based on Deterministic Lateral Displacement.

Mammalian blood cell separation methods contribute to improving the diagnosis and treatment of animal and human diseases. Microfluidic deterministic lateral displacement (DLD) devices can sort cells based on their particle diameter. We developed microfluidic DLD devices with poly(propylene)-based resin and used them to separate bovine and human red blood cells (RBCs) and white blood cells (WBCs) without electric devices. To determine the critical cut-off diameter (Dc) of these devices, we used immunobeads with a diameter of 1-20 μm. The Dc values of the microfluidic DLD devices for the immunobeads in the experiments were similar to the calculated Dc values (8-10 μm). Results from bovine blood cell separation experiments suggest that lymphocytes and neutrophils can be separated from diluted, whole blood. Human RBCs were occasionally observed in the left outlet where larger particles with diameters closer to the Dc value were collected. Based on the Dc values, human neutrophils were sorted to the left outlet, whereas lymphocytes were observed in both outlets. Although microfluidic channel optimization is required for the concentration of sorted cells, the microfluidic DLD device prepared with a poly(propylene)-based resin has the potential for clinical use.

Increasing flow rates in polydimethylsiloxane-based deterministic lateral displacement devices for sub-micrometer particle separation

In this study, we show the design and manufacturing of microfluidic deterministic lateral displacement (DLD) devices for sub-micrometer particle separation. For that purpose, devices with pillar gaps of 4 µm and a periodicity of 50 were designed. After photolithographic manufacturing of SU-8 masters with different heights (15 and 30 µm) and vertical sidewalls for soft-lithographic replication with polydimethylsiloxane (PDMS) the influence of flow rate on the separation efficiency of 0.45 and 0.97 µm particles was investigated. The 15 µm devices were operated at 0.125 and 0.5 µl/min sample flow rate and the 30 µm devices at 0.5 and 2.0 µl/min, respectively. Excellent separation efficiencies were observed for both device heights at the lower sample flow rates, while separation efficiencies decreased at the respective higher sample flow rates. The decrease in separation efficiency was attributed to deformation of the soft PDMS pillars, which causes an increase in pillar gaps at the higher sample flow rates as shown by microscopy imaging. The advantage of the 30 µm devices over the 15 µm devices is clearly shown by the separation of 0.45 and 0.97 µm particles at 0.5 µl/min. Due to reduced hydrodynamic resistance in the 30 µm devices and thus less pillar deformation, the displacement efficiency of 0.97 µm particles was above 99% compared to 46–57% for the 15 µm devices. Our 30 µm devices demonstrated excellent separation at a tenfold higher sample flow rate with 0.5 µl/min compared to comparable PDMS-based devices operating in the same size regime.

Experimental Characterization of Oil/Gas Interface Self-Adjustment in CO2-Assisted Gravity Drainage for Reverse Rhythm Reservoir

Worldwide practices have proven that gas-assisted gravity drainage can obviously enhance oil recovery, and this technology can be especially effective for reservoirs with a thick formation and large inclination angle. For the successful implementation of this process, a key technology is the stable control of gas–oil interface during gas injection. For a detailed exploration of this technique, a three-stage permeable visual model was designed and manufactured, with permeability decreasing from top to bottom, thus, a reverse rhythm reservoir was effectively modeled. Then the experiment concerning CO2-assisted gravity drainage was carried out with the adoption of a self-developed micro visual displacement device. This study mainly focused on the micro migration law of gas–oil interface and the development effects of CO2-assisted gravity drainage. According to the experiments, CO2 fingering somewhat happens in the same permeable layer from the beginning of gas injection. However, phenomena of “wait” and “gas–oil interface self-adjustment” occur instead of flowing into the next layer when the injected CO2 reaches the boundary of the next lower permeability layer through the dominant channel. By the “gas–oil interface self-adjustment”, the injected CO2 first enters into the pores of the relative higher permeability layer to the greatest extent, and thus expands the sweep volume. Futhermore, in the process of CO2 injection, obvious gas channeling occurs in the low permeability layer directly connected to the outlet, resulting in low sweep efficiency and poor development effect. After connecting the core with lower permeability at the outlet, the development indexes of the model, such as the producing degree of the low permeability layer, the oil recovery before and after gas breakthrough, are significantly improved, and the recovery degrees of the medium permeability layer and the high permeability layer are also improved, and the overall recovery factor is increased by 12.38%. This “gas–oil interface self-adjustment” phenomenon is explained reasonably from the two scales of macroscopic flow resistance and microscopic capillary force. Finally, the enlightenments of the new phenomenon are expounded on the application of gas-assisted gravity drainage on site and the treatment of producers with gas breakthrough in gas injection development.