Dispersal Patterns Research Articles

Costing of a Combination Intervention (Kyaterekera) Addressing Sexual Risk-Taking Behaviors among Vulnerable Women in Southern Uganda.

In Uganda, women engaged in sex work (WESW) are a marginalized population at the intersection of multiple vulnerabilities. The Kyaterekera intervention is targeted at WESW in Rakai and the greater Masaka regions in Uganda and combines a traditional HIV risk-reduction approach with a savings-led economic empowerment intervention and financial literacy training. We estimated the economic costs of the Kyaterekera intervention from a program provider perspective using a prospective activity-based micro-costing method. All program activities and resource uses were measured and valued across the control arm receiving a traditional HIV risk-reduction intervention and the treatment arm receiving a matched individual development savings account and financial literacy training on top of HIV risk reduction. The total per-participant cost by arm was adjusted for inflation and discounted at an annual rate of 3% and presented in 2019 US dollars. The total per-participant costs of the control and intervention arms were estimated at $323 and $1,435, respectively, using the per-protocol sample. When calculated based on the intent-to-treat sample, the per-participant costs were reduced to $183 and $588, respectively. The key cost drivers were the capital invested in individual development accounts and personnel and transportation costs for program operations, linked to WESW's higher mobility and the dispersed pattern of hot spot locations. The findings provide evidence of the economic costs of implementing a targeted intervention for this marginalized population in resource-constrained settings and shed light on the scale of potential investment needed to better achieve the health equity goal of HIV prevention strategies.

Spatial Distribution and Ecological Determinants of Coexisting Hybrid Oak Species: A Study in Yushan's Mixed Forest.

Ecological niche partitioning is crucial in reducing interspecific competition, fostering species coexistence, and preserving biodiversity. Our research, conducted in a hybrid mixed oak forest in Yushan, Jiangsu, China, focuses on Quercus acutissima, Q. variabilis, Q. fabri, and Q. serrata var. brevipetiolata. Using Point Pattern Analysis, we investigated the spatial relationships and ecological trait autocorrelation, including total carbon (TC), nitrogen (TN), phosphorus (TP), potassium (TK), and breast height diameter (DBH). Our findings show aggregated distribution patterns within the oak populations. The Inhomogeneous Poisson Point model highlights the impact of environmental heterogeneity on Q. variabilis, leading to distinct distribution patterns, while other species showed wider dispersion. This study reveals aggregated interspecific interactions, with a notable dispersal pattern between Q. acutissima and Q. variabilis. We observed significant variability in nutrient elements, indicating distinct nutrient dynamics and uptake processes. The variations in total carbon (TC), nitrogen (TN), phosphorus (TP), and potassium (TK) suggest distinct nutrient dynamics, with TK showing the highest variability. Despite variations in TC, TK, and TP, the species did not form distinct classes, suggesting overlapping nutritional strategies and environmental adaptations. Furthermore, spatial autocorrelation analysis indicates strong positive correlations for DBH, TC, and TP, whereas TK and TN correlations are non-significant. The results suggest habitat filtering as a key driver in intraspecific relationships, with a finer spatial scale of ecological niche division through TC and TP, which is crucial for maintaining coexistence among these oak species.

Small‐scale dispersal and gene flow of the agricultural pest two‐spotted spider mite in apple orchards: Implication from landscape ecological and population genetic analyses

AbstractThe two‐spotted spider mite, Tetranychus urticae Koch (Acarina, Tetranychidae), is a notorious agricultural pest known for its high dispersal ability. Understanding the dispersal patterns of T. urticae is crucial for studying its population dynamics and assessing the risk of gene dispersal related to acaricide resistance. We investigated the impact of surrounding landscapes on T. urticae occurrence and detected gene flow between nearby apple orchards. Land use data within 0.2–2 km radius buffers of the orchards were extracted and summarized using principal component analysis. The effects of landscape composition on the occurrence of T. urticae in apple orchards were analyzed using a hierarchical Bayesian model that considers spatial autocorrelation. In addition, we conducted a population genetic analysis on samples collected from five orchards. The results indicate that the best model was one at a 200‐m scale, revealing a positive correlation between forest area ratio and T. urticae occurrence. The population genetic analysis demonstrated distinct genetic structures within T. urticae populations across different orchards. However, we estimated gene flow among some orchards that were geographically close. These findings suggest that T. urticae may migrate from surrounding landscapes, particularly forests, and nearby orchards to apple orchards. These findings have substantial implications for developing control units based on the gene flow range of the two‐spotted spider mite, as well as for assessing the risk of infestation in each orchard.

Paleomagnetic secular variation and revised chronostratigraphy of Bering Sea (IODP expedition 323) deep-sea sediments (MIS 5)

This study presents new high-resolution, full-vector paleomagnetic secular variation (PSV) records from IODP Expedition 323 Sites U1339, U1343, U1344, and U1345 in the Bering Sea (51°N-60°N) during Marine Isotope Stage 5 (71–130 ka). The chronology of the records has been determined by oxygen isotope stratigraphy with an age uncertainty of ∼±2000 years. The data come from shipboard paleomagnetic measurements of deep-sea sediments with accumulation rates of 26–54 cm/ky. The sample interval in the composite records is 5 cm with a time resolution of ±100–200 years. The composite PSV records for each site were developed by correlation and analysis of 3–4 paleomagnetic records from individual holes at each site. The composite records are a combination of the best correlatable PSV data from each site. We have been able to significantly correlate the PSV records from all four sites. We have identified 56 inclination features (highs/lows) and 54 declination features (east/west extremes) in all the records. Relative paleointensity has been determined by normalizing the sediment natural remanence, after 20-mT af demagnetization, to magnetic susceptibility. This normalization process is complicated by environmental variability, thus our relative paleointensity records are limited to large-scale correlations among the four sites. We correlated our relative paleointensity records to the global PISO-1500 paleointensity record that is itself dated by oxygen isotope stratigraphy. We can identify 6 key intensity highs/lows in our composite records and the PISO-1500 record that provide additional dating isochrons for our records. There are three short magnetic field excursions recorded with replication at these four sites – The youngest excursion occurred at 100 ± 2 ka and is associated with the Fram Strait Excursion. Two older excursions occurred at 116 ± 2 ka and 119.5 ± 2 ka. We associate both of these with the Blake Event. All three of the excursions have very short time intervals (∼200–400 yrs) with somewhat out-of-phase inclination and declination variability. All three of these are Class I excursions. We have studied the statistical characteristics our composite records by calculating the 3ky and 9ky averaged inclinations, declinations, unit vectors, and relative paleointensity over 71–130 ka after removing all excursional directions. The averaged inclinations and declinations are consistent among the four sites. The averaged angular dispersion is bimodal in its overall pattern. Most of the time angular dispersion is low (∼±10°–12°) except for three shorter intervals that have significantly higher angular dispersion (∼±24–30°) and lower paleointensities. The high angular dispersion intervals are associated with the three short excursional intervals. The timing and pattern of interrelated high angular dispersion, low paleointensity, and excursions is synchronous with the PSV pattern recorded during MIS 5 in the central North Atlantic Ocean. We think this defines a distinctive low-intensity/low-energy state for the geomagnetic field.

Abstract 3648: The spatial organization of cells expressing MYC and BCL2 affects immune microenvironment composition and prognosis in DLBCL

Abstract Point process analyses are widely used in ecology and geography to understand the impact and relevance of spatial distribution of points in a complex system. To date, only few studies have explored point process analyses in the context of tumor heterogeneity. Malignancies often show variable patterns of cellular distribution, and the relationship of these topographic variables with underlying biological processes and clinical outcomes is not well understood. Here, we performed spatial point process analyses in diffuse large B-cell lymphoma (DLBCL), leveraging advances in multiplexed immunohistochemistry and cellular phenotyping, with downstream multi-omic and clinicopathological analyses. We have demonstrated that survival in DLBCL is strongly associated with the fraction of malignant cells showing co-expression of the oncogenes MYC and BCL2 in the absence of BCL6 (M+2+6-). These cells display non-random spatial organization within tumor infiltrates, deviating from a poisson distribution. We therefore modeled the spatial organization of M+2+6- cells within DLBCL, using x-y coordinate information from multiplexed fluorescent immunohistochemistry (mfIHC) in four independent cohorts (N=449 patients). We derived spatial point patterns, upon which Geyers point process models were applied. We observed that patients could be divided into two groups based on these: one group showed “clustered” spatial organization, while the other displayed a “dispersed” M+2+6- cell distribution. Cases with predominantly “dispersed” M+2+6- cells showed shorter overall survival in all analyzed cohorts (P < 0.05 in 4/4 cohorts). We performed multi-omic analyses to identify potential biological explanations for this association between topography and clinical outcome. Using digital spatial profiling of the transcriptome from CD20, CD3 and CD68 compartments in 47 DLBCL samples, we observed that lymphomas with an M+2+6- ‘dispersed’ phenotype had an immunologically cold microenvironment enriched in Tregs and exhausted CD4+ and CD8+ T cells. Through an integration with single-cell transcriptomic analyses (N=22 samples) we observed that malignant B-cells from cases with M+2+6- ‘dispersed’ phenotype expressed genes implicated in cell migration and adhesion, but also in potentially targetable immune checkpoints such as LAG3. In summary, by integrating multiplexed immunophenotyping and point process analysis with molecular profiling in the setting of DLBCL, we show that a dispersed pattern of distribution of the M+2+6- subclonal fraction uniquely confers poor prognosis, and associates with a potentially reversible immune cold microenvironment composition. This represents the first demonstration that the spatial distribution of malignant cell subpopulations in lymphoma can embody biological and clinical significance. Citation Format: Shruti Shridar, Michal Marek Hoppe, Patrick Jaynes, Gayatri Kumar, Siddham Jasoria, Ziwei Meng, Vaibhav Rajan, Kasthuri Kannan, Claudio Tripodo, Anand Devaprasath Jeyasekharan. The spatial organization of cells expressing MYC and BCL2 affects immune microenvironment composition and prognosis in DLBCL [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3648.

Dispersal patterns of the first White-backed Vulture (Gyps africanus) GPS-tracked in Iberia

Dispersal patterns of the first White-backed Vulture (Gyps africanus) GPS-tracked in Iberia

LOCATE v1.0: numerical modelling of floating marine debris dispersion in coastal regions using Parcels v2.4.2

Abstract. The transport mechanisms of floating marine debris in coastal zones remain poorly understood due to complex geometries and the influence of coastal processes, posing difficulties in incorporating them into Lagrangian numerical models. The numerical model LOCATE overcomes these challenges by coupling Eulerian hydrodynamic data at varying resolutions within nested grids using Parcels, a Lagrangian particle solver, to accurately simulate the motion of plastic particles where a high spatial coverage and resolution are required to resolve coastal processes. Nested grids performed better than a coarse-resolution grid when analysing the model's dispersion skill by comparing drifter data and simulated trajectories. A sensitivity analysis of different beaching conditions comparing spatiotemporal beaching patterns demonstrated notable differences in the land–water boundary detection between nested hydrodynamic grids and high-resolution shoreline data. The latter formed the basis for a beaching module that parameterised beaching by calculating the particle distance to the shore during the simulation. A realistic debris discharge scenario comparison around the Barcelona coastline using the distance-based beaching module in conjunction with nested grids or a coarse-resolution grid revealed very high levels of particle beaching (>91.5%) in each case, demonstrating the importance of appropriately parameterising beaching at coastal scales. In this scenario, high variability in particle residence times and beaching patterns was observed between simulations. These differences derived from how each option resolved the shoreline, with particle residence times being much higher in areas of intricate shoreline configurations when using nested grids, thus resolving complex structures that were undetectable using the coarse-resolution grid. LOCATE can effectively integrate high-resolution hydrodynamic data within nested grids to model the dispersion and deposition patterns of particles at coastal scales using high-resolution shoreline data for shoreline detection uniformity.

Relationship between genetic diversity and morpho-functional characteristics of flight-related traits in Triatoma garciabesi (Hemiptera: Reduviidae)

BackgroundTriatoma garciabesi, a potential vector of the parasitic protozoan Trypanosoma cruzi, which is the causative agent of Chagas disease, is common in peridomestic and wild environments and found throughout northwestern and central Argentina, western Paraguay and the Bolivian Chaco. Genetic differentiation of a species across its range can help to understand dispersal patterns and connectivity between habitats. Dispersal by flight is considered to be the main active dispersal strategy used by triatomines. In particular, the morphological structure of the hemelytra is associated with their function. The aim of this study was to understand how genetic diversity is structured, how morphological variation of dispersal-related traits varies with genetic diversity and how the morphological characteristics of dispersal-related traits may explain the current distribution of genetic lineages in this species.MethodsMales from 24 populations of T. garciabesi across its distribution range were examined. The cytochrome c oxidase I gene (coI) was used for genetic diversity analyses. A geometric morphometric method based on landmarks was used for morpho-functional analysis of the hemelytra. Centroid size (CS) and shape of the forewing, and contour of both parts of the forewing, the head and the pronotum were characterised. Length and area of the forewing were measured to estimate the aspect ratio.ResultsThe morphometric and phylogenetic analysis identified two distinct lineages, namely the Eastern and Western lineages, which coincide with different ecological regions. The Eastern lineage is found exclusively in the eastern region of Argentina (Chaco and Formosa provinces), whereas the Western lineage is prevalent in the rest of the geographical range of the species. CS, shape and aspect ratio of the hemelytra differed between lineages. The stiff portion of the forewing was more developed in the Eastern lineage. The shape of both portions of the hemelytra were significantly different between lineages, and the shape of the head and pronotum differed between lineages.ConclusionsThe results provide preliminary insights into the evolution and diversification of T. garciabesi. Variation in the forewing, pronotum and head is congruent with genetic divergence. Consistent with genetic divergence, morphometry variation was clustered according to lineages, with congruent variation in the size and shape of the forewing, pronotum and head.Graphical

Effectiveness of human immunodeficiency virus prevention strategies by mapping the geographic dispersion pattern of human immunodeficiency virus prevalence in Nanning, China

BackgroundThe Guangxi government initiated two rounds of the Guangxi AIDS Conquering Project (GACP) in 2010 (Phase I) and 2015 (Phase II) to control human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) epidemics. However, the effectiveness of GACP in HIV prevention and treatment has rarely been reported. This study aimed to assess the effectiveness of the GACP implemented in Guangxi, China and provide data for strategy and praxis improvements to achieve Joint United Nations Programme on HIV/AIDS (UNAIDS) 95-95 targets.MethodsWe used spatial approaches to trace the spatiotemporal distribution properties, epidemic trends, and correlation between macroscopic factors and HIV incidence using data from the Chinese HIV/AIDS case reporting system to explore the effects of the GACP.ResultsDuring the GACP era, the HIV epidemic stabilized in urban centers, showing a downward trend in the Hengzhou and Binyang Counties in the eastern region, whereas it continued to increase in rural areas of the northwest region, such as the Long’an, Mashan, Shanglin, and Wuming Districts. The linear directional mean (LDM) of HIV infection reported cases displayed a southeast–northwest direction, with an LDM value of 12.52°. Compared with that in Phase I, Hengzhou withdrew from the high-high clustering area, and the west–north suburban counties pulled out the low-low clustering area during Phase II. Significant HIV clusters were identified in the eastern region during Phase I, whereas these clusters emerged in the northwestern areas during Phase II. Regarding HIV, socioeconomic status, population mobility, and medical care levels were the key social drivers of heterogeneous spatial distribution.ConclusionsThe GACP assisted in effectively managing the HIV epidemic in urban and eastern areas of Nanning City. However, prevention and control efforts in rural regions, particularly those located in the northwest, may not have yielded comparable outcomes. To address this disparity, allocating additional resources and implementing tailored intervention measures for these rural areas are imperative.

Lessons from the calibration and sensitivity analysis of a fish larval transport model

Numerous fish populations show strong year-to-year variations in recruitment. The early life stages play a crucial role in determining recruitment and dispersal patterns. A helpful tool to understand recruitment and dispersal involves simulations with a Lagrangian transport model, which results from the coupling between a hydrodynamic model and an individual-based model. Larval transport models require sound knowledge of the biological processes governing larval dispersal, and they may be highly sensitive to the parameters selected. Various assumptions about larval traits, behaviour and other model parameters can be tested by comparing simulation results with field data to identify the most sensitive parameters and to improve model calibration. This study shows that biological parameterization is more important than inter-annual variability in explaining the year-to-year differences in larval recruitment of common sole in the North Sea and the eastern English Channel. In contrast, year-to-year variability of connectivity leads to higher variability than changes in the biological parameters. The most influential parameters are pelagic larval duration, spawning period and mortality. Calibration over a 12 yr recruitment survey shows that a scenario with low mortality associated with a long larval duration and behaviour involving nycthemeral and tidal migration best reproduces the observations. This research provides insights into factors influencing fish dispersal and recruitment, suggesting a strategy for enhancing the accuracy of models in upcoming studies. The study supports the improvement of larval dispersal modelling by incorporating an easily applicable sensitivity analysis for both calibration and validation. Incorporating sensitivity analyses enhances larval dispersal models, providing performing tools that can contribute to informed fisheries management and understanding of recruitment variability.

The effect of particle size on aluminum dust dispersion based on numerical simulation

This study aimed to investigate the dispersion pattern of aluminum dust with different particle sizes in 20 L vessels by numerical simulations. Using coupled Eulerian and Lagrangian methods, the system of Navier–Stokes equations and DPM momentum balance equations were solved to obtain the dispersion process of aluminum dust with different particle sizes (25, 38, 45, 60, and 75 μm). A standard deviation of particle concentration (Spc) was proposed to characterize dispersity of dust; the smaller Spc is, the better the dispersity of dust is. On the basis of Spc, after the sample reached uniform distribution, the dust with large particle size could maintain uniform dispersion for a certain time period. By contrast, the dust with small particle size, which was more affected by the central turbulence than large dust particles, had difficulty in maintaining uniform dispersion. In addition, the use of rebound nozzles for dust dispersion led to a large turbulence intensity gradient, which significantly influenced the dust dispersion process, especially on the small particle sizes. A large amount of dust was concentrated in the near-wall region, resulting in the concentration of dust in the center of the chamber being lower than expected.

Molecular and entomological surveillance of malaria vectors in urban and rural communities of Benguela Province, Angola

BackgroundMalaria is a major public health problem in Angola, with Anopheles gambiae sensu lato (s.l.) and An. funestus s.l. being the primary vectors. This study aimed to clarify the information gaps concerning local Anopheles mosquito populations. Our objectives were to assess their abundance, geographical dispersion, and blood-feeding patterns. We also investigated their insecticide resistance. Molecular methods were used to identify sibling species, determine the origin of blood meals, measure Plasmodium falciparum infection rates, and detect the presence of knockdown resistance (kdr) mutations.MethodsAdult mosquitoes were collected indoors using CDC light traps from nine randomly selected households at two sentinel sites with distinct ecological characteristics. The samples were collected from 1 February to 30 June 2022. Anopheles mosquitoes were morphologically identified and subjected to molecular identification. Unfed Anopheles females were tested for the presence of P. falciparum DNA in head and thorax, and engorged females were screened for the source of the blood meals. Additionally, members of An. gambiae complex were genotyped for the presence of the L1014F and L1014S kdr mutations.ResultsIn total, 2226 adult mosquitoes were collected, including 733 Anopheles females. Molecular identification revealed the presence of Anopheles coluzzii, An. gambiae senso stricto (s.s.), An. arabiensis, and An. funestus s.s. Notably, there was the first record of An. coluzzii/An. gambiae s.s. hybrid and An. vaneedeni in Benguela Province. Plasmodium falciparum infection rates for An. coluzzii at the urban sentinel site and An. funestus s.s. at the rural site were 23.1% and 5.7%, respectively. The L1014F kdr mutation was discovered in both resistant and susceptible An. coluzzii mosquitoes, while the L1014S mutation was detected in An. gambiae s.s. for the first time in Benguela Province. No kdr mutations were found in An. arabiensis.ConclusionsThis study provides valuable insights into the molecular characteristics of malaria vectors from the province of Benguela, emphasising the need for continuous surveillance of local Anopheles populations regarding the establishment of both kdr mutations for tailoring vector control interventions.Graphical abstract

Prediction of new 212 M2AB2 borides as a promising candidate for future engineering: DFT calculations

The elemental diversity is critical for identifying ternary MAB phases with exceptional properties by tuning bonding types and strength between constitutive atoms. The interactions between M and A atoms significantly impact the physical and chemical properties of MAB phases. M2AB2 (where M = Hf, Zr; A = Ga, Ge) was studied using first-principles density functional theory (DFT). Lattice constants (a, c) and internal coordinates have been found after optimization. The lattice parameters in terms of structure exhibit conformity with findings from earlier research. The electronic band structure (EBS) density of states (DOS) and density of states (DOS) have been determined. According to the band structures and density of states investigation, these compounds are electrical conductors. The elastic constants were computed using the stress-strain method. The computed elastic constants and phonon dispersion patterns support this newly found compound's mechanical and dynamical stability. The isotropic elastic moduli bulk modulus (B), shear modulus (G), Young's modulus (E), Poisson's ratio (v), and Vickers hardness (Hv) have been explored within the framework of the Voigte-Reusse-Hill approximation. Specific heat capacity, Debye temperature, entropy, Helmholtz free energy, and internal energy changes of M2AB2 (where M = Hf, Zr; A = Ga, Ge) compounds were investigated using thermodynamic characteristics. The minimum thermal conductivity (Kmin) and melting temperature (Tm) of the compounds have also been computed to assess whether they are suitable as thermal barrier coating (TBC) materials or for high-temperature applications. These compounds' optical properties have been studied, demonstrating their usefulness as coating materials for reducing solar heat absorption. The examined physical characteristics of M2AB2 (where M = Hf, Zr; A = Ga, Ge) are contrasted with those of comparable 212 and 211 MAB phases.

Temporal Dynamics and Dispersal Patterns of the Primary Inoculum of Coniella diplodiella, the Causal Agent of Grape White Rot.

Grape white rot can cause considerable yield losses in viticulture areas worldwide and is principally caused by Coniella diplodiella. The fungus overwinters in berry mummies on the soil surface or on the trellis and produces pycnidia and conidia that serve as primary inoculum. However, little is known about the temporal dynamics and dispersal pattern of C. diplodiella conidia. In this study, we investigated the production and dispersal of C. diplodiella conidia from a primary inoculum source, namely, affected mummified berries that overwintered in two vineyards in northern Italy in 2021 and 2022. Conidia of C. diplodiella were repeatedly produced in berry mummies from the budburst of vines to harvesting, with approximately 50 and 75% of the total conidia in a season being produced before fruit set and véraison, respectively. The production dynamics of C. diplodiella conidia over time were described by a Weibull equation in which the thermal time is the independent variable, with a concordance correlation coefficient of ≥0.964. A rainfall cutoff of ≥0.2 mm provided an overall accuracy of ≥0.86 in predicting conidial dispersal through rain splashes from berry mummies on the soil surface, with the number of dispersed conidia increasing with the amount of rainfall. The dispersal of conidia from mummies on the trellis by washing with rain required at least 6.1 mm of rain. The proposed mathematical equations and rain cutoffs can be used to predict periods with a high dispersal risk of C. diplodiella.

Free vibration analysis of functionally graded graphene platelet-reinforced metal foam doubly curved panel

In this research, free vibration characteristics of functionally graded metal foam doubly curved panels reinforced with graphene platelets and with porosities have been surveyed. Halpin Tsai's approach is utilized for extracting the effective Young modulus of porous metal foam nanocomposite and also the effective density of nanocomposite porous doubly curved shell panel is estimated by using an extended rule of mixture. The FSDT hypothesis is utilized for determining the displacement field and the Finite element and Hamilton principle are utilized for deriving the mass and stiffness matrices of the structure. Finally, the influences of several variables such as porosity distribution, porosity coefficient, GPL dispersion pattern, the weight fraction of Nanofillers, and span angles on the free vibrations characteristics of doubly curved shell panels with FG porosities and reinforced by graphene platelet have been reported in detail.

Acetylcholine esterase of Drosophila melanogaster: a laboratory model to explore insecticide susceptibility gene drives.

One of the proposed applications of gene drives has been to revert pesticide resistant mutations back to the ancestral susceptible state. Insecticides that have become ineffective because of the rise of resistance could have reinvigorated utility and be used to suppress pest populations again, perhaps at lower application doses. We have created a laboratory model for susceptibility gene drives that replaces field-selected resistant variants of the acetylcholine esterase (Ace) locus of Drosophila melanogaster with ancestral susceptible variants. We constructed a CRISPR/Cas9 homing drive and found that homing occurred in many genetic backgrounds with varying efficiencies. While the drive itself could not be homozygous, it converted resistant alleles into susceptible ones and produced recessive lethal alleles that could suppress populations. Our studies provided evidence for two distinct classes of gene drive resistance (GDR): rather than being mediated by the conventional non-homologous end-joining (NHEJ) pathway, one seemed to involve short homologous repair and the other was defined by genetic background. Additionally, we used simulations to explore a distinct application of susceptibility drives; the use of chemicals to prevent the spread of synthetic gene drives into protected areas. Insecticide susceptibility gene drives could be useful tools to control pest insects however problems with particularities of target loci and GDR will need to be overcome for them to be effective. Furthermore, realistic patterns of pest dispersal and high insecticide exposure rates would be required if susceptibility were to be useful as a 'safety-switch' to prevent the unwanted spread of gene drives. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Spatial analysis of rabies-transmitting animal bite cases in North Tapanuli Regency, North Sumatera Province in 2016-2020

Purpose: Rabies is a priority strategic disease because it has an impact on socio-economic and public health. This study aims to describe the incidence of rabies-transmitting animal bite cases in North Tapanuli Regency in 2016-2020, contribute to understanding the dynamics of cases, and provide effective information in controlling cases of animal bites that transmit rabies.
 Methods: Ecological study using aggregated data. The data was collected from monthly reports of rabies cases at the North Tapanuli Regency Health Service in 2016-2020. As a unit analysis, all districts are used. QGIS and GeoDA software are used for data analysis. Through GeOda software, spatial autocorrelation analysis was carried out globally using Moran's I test and locally using the LISA test.
 Results: Rabies-transmitting animal bite cases had a dispersed pattern in 2016 (I<E[I]) and a clustered pattern (I>E[I]). Globally, there is positive spatial autocorrelation in rabies-transmitting animal bite cases between districts in North Tapanuli Regency in 2017 and 2020 (p-value < 0.05). Moran's cluster map shows that locally in 2016-2020, the districts in the quadrant I (High-High) are Siborongborong and Sipoholon. Quadrant II (Low-High) is Adiankoting and Pagaran. Quadrant III (Low-Low) is Pangaribuan, and quadrant IV (High-Low) is Pahae Jae and Pangaribuan.
 Conclusion: Globally, cases of animal bites that transmit rabies in North Tapanuli Regency occur in clusters. It means that the number of bite cases in one area is related to the number of cases in other nearby areas. Therefore, rabies control program interventions in areas with high observation values must be prioritized so they do not spread to areas with low observation values.

Nonlinear thermal buckling analysis of temperature-dependent porous annular and circular microplates reinforced by graphene platelets by using isogeometric analysis method

In this research, by using isogeometric analysis (IGA) method, nonlinear thermal buckling analysis of porous circular and annular microplates reinforced by graphene platelets (GPLs) under uniform temperature rise is performed by taking both the temperature dependence of the material properties and the size effects into account. The closed-cell metal foam scheme combined with the Halpin-Tsai micromechanics model and the rule of mixture is utilized to identify the effective material properties of the porous nanocomposites for three GPL dispersion patterns and three porosity distributions. With the aid of the virtual work principle, the classical and the non-classical motion equilibrium formulation based on the higher-order shear deformation theory in conjunction with the modified strain gradient (MSG) theory for the nonlinear temperature-dependent buckling behaviour are established. The higher-order NURBS basis functions capable of accomplishing easily the smoothness of arbitrary continuity order are successfully utilized to solve the nonlinear governing equations by satisfying the C2-continuity of the displacement field required by the MSG theory. The thermal buckling behaviour is assessed in detail by using various parameters such as the thickness-to-radius ratio, the porosity coefficient, the GPL weight fraction and the material length scale as well as the porosity distribution scheme and the GPL dispersion pattern. The results show the significance of incorporating the temperature dependency of the material properties when analyzing the thermal buckling behaviour and present the combination scheme of porosity and GPL distributions for enhancing the thermal buckling resistance.

Synthesis and characterization of polylactic acid‐lecithin‐starch bioplastic film

AbstractPlastic‐induced environmental issues could be solved using biomaterials, such as polylactic acid (PLA) film. PLA film is a costly solution suggesting the need to add less expensive starch. However, PLA and starch do not mix due to their diverging water behavior. In this study, we evaluated the impact of lecithin as a compatibilizer in varying ratio of PLA and starch film. The results show that inclusion of lecithin in PLA/starch composite leads to enhanced mechanical properties compared with the composite without lecithin. All films' thermal properties were stable but the thermograph of PLA/starch display two peaks whose distance is impacted by lecithin. In addition, morphology and functional group fingerprints revealed that the addition of lecithin improved the interfacial adhesion between the two polymers. Lecithin influenced the positioning and dispersion pattern of starch granules and distinct transmittance characteristics. The improved compatibility of PLA/starch makes the resulting films less susceptible to water penetration and dissolution. This work demonstrated the possibility of using lecithin as emulsifier between PLA and starch which could expand the application of PLA/starch film especially in packaging industries and bale net wrapping.

The Effects of Ambient Temperature and Atmospheric Humidity on the Diffusion Dynamics of Hydrogen Fluoride Gas Leakage Based on the Computational Fluid Dynamics Method.

In order to investigate the impact of environmental temperature and atmospheric humidity on the leakage and diffusion of hydrogen fluoride (HF) gas, this study focused on the real scenario of an HF chemical industrial park. Based on the actual dispersion scenario of HF gas, a proportionally scaled-down experimental platform for HF gas leakage was established to validate the accuracy and feasibility of numerical simulations under complex conditions. Using the validated model, the study calculated the complex scenarios of HF leakage and diffusion within the temperature range of 293 K to 313 K and the humidity range of 0% to 100%. The simulation results indicated that different environmental temperatures had a relatively small impact on the hazardous areas (the lethal area, severe injury area, light injury area, and maximum allowable concentration (MAC) area) formed by HF gas leakage. At 600 s of dispersion, the fluctuation range of hazardous area sizes under different temperature conditions was between 3.11% and 13.07%. In contrast to environmental temperature, atmospheric relative humidity had a more significant impact on the dispersion trend of HF leakage. Different relative humidity levels mainly affected the areas of the lethal zone, light injury zone, and MAC zone. When HF continued to leak and disperse for 600 s, compared to 0% relative humidity, 100% relative humidity reduced the lethal area by 35.7%, while increasing the light injury area and MAC area by 27.26% and 111.6%, respectively. The impact on the severe injury area was relatively small, decreasing by 1.68%. The results of this study are crucial for understanding the dispersion patterns of HF gas under different temperature and humidity conditions.