Purpose: The purpose of the study is that the difference of the activation of the prefrontal cortex is made clear in order to know the ability for EMDR to influence the anxiety control. Methods: In order to achieve the purpose of this research, the eight undergraduate athletes were chosen, using discussion of anxiety, CSAI-2, SIAQ. And OBELAB fNIRS, a kind of instrument, and Matlab software were used for the experiment. The activation of the prefrontal cortex was measured in chronological order, and base line, pre-imagery, bilaterality eye movement program, post-imagery were progressed one after another. Result: First, the result of using imagery to cause anxiety was that the overall activation of the prefrontal cortex is decreased. Second, adjusting the bilaterality eye movement program cause the activation of the right prefrontal cortex to be decreased in pre-imagery and post-imagery. Finally, when each time of the bilaterality eye movement is measured, we can identify the result that the right prefrontal cortex is activated. Conclusion: This result shows that EDMR can quickly reprocess into the positive metal status the negative one caused by the negative memory about anxiety. This study shows the possibility that EDMR can be used when adjusting the unexpected anxiety which happens during the specific situation, sports.