Discovery Of Mimivirus Research Articles

Les virus géants : une histoire Marseillaise

Marseilles, Giant virus, Ameoba, Evolution, Mimivirus For twenty years, two Marseille laboratories have collaborated, then competed, in the exploration of a new field of research, that of viruses infecting amoebae, which have turned out to be giants. Stemming from the unexpected result of an initially misinterpreted experiment, this research theme, for which neither of the two laboratories was prepared, has profoundly impacted virology, right down to the very concept of a virus. Since the discovery of Mimivirus, the first giant virus, these now come in 4 distinct families. Here is a brief history of their discoveries.

Morphological and Genomic Features of the New Klosneuvirinae Isolate Fadolivirus IHUMI-VV54.

Since the discovery of Mimivirus, viruses with large genomes encoding components of the translation machinery and other cellular processes have been described as belonging to the nucleocytoplasmic large DNA viruses. Recently, genome-resolved metagenomics led to the discovery of more than 40 viruses that have been grouped together in a proposed viral subfamily named Klosneuvirinae. Members of this group had genomes of up to 2.4Mb in size and featured an expanded array of translation system genes. Yet, despite the large diversity of the Klosneuvirinae in metagenomic data, there are currently only two isolates available. Here, we report the isolation of a novel giant virus known as Fadolivirus from an Algerian sewage site and provide morphological data throughout its replication cycle in amoeba and a detailed genomic characterization. The Fadolivirus genome, which is more than 1.5Mb in size, encodes 1,452 predicted proteins and phylogenetic analyses place this viral isolate as a near relative of the metagenome assembled Klosneuvirus and Indivirus. The genome encodes for 66 tRNAs, 23 aminoacyl-tRNA synthetases and a wide range of transcription factors, surpassing Klosneuvirus and other giant viruses. The Fadolivirus genome also encodes putative vacuolar-type proton pumps with the domains D and A, potentially constituting a virus-derived system for energy generation. The successful isolation of Fadolivirus will enable future hypothesis-driven experimental studies providing deeper insights into the biology of the Klosneuvirinae.

Giant viruses that create their own genes

Since 2003 and the discovery of Mimivirus, the saga of giant viruses continues with the isolation of new amoeba viruses, which are now divided into seven distinct families, the origin (s) of which are still mysterious and controversial. Thanks to the isolation of 3 new members of the Pandoraviridae family, whose micrometric particles and genomes of more than 2 megabases encroach on the cellular world, we carried out a stringent re-analysis of their gene contents, using a combination of transcriptomic, proteomic and bioinformatic approaches. We concluded that the only scenario capable of accounting for the distribution and the huge proportion of orphan genes ("ORFans") that characterize Pandoraviruses is that they were created de novo within the intergenic regions. This process, perhaps shared among other large DNA viruses, challenges the central paradigm of molecular evolution according to which all genes / proteins have an ancestry history.

Deciphering viral presences: two novel partial giant viruses detected in marine metagenome and in a mine drainage metagenome

Nucleo-cytoplasmic large DNA viruses are doubled stranded DNA viruses capable of infecting eukaryotic cells. Since the discovery of Mimivirus and Pandoravirus, there has been no doubt about their extraordinary features compared to “classic” viruses. Recently, we reported the expansion of the proposed family Pithoviridae, with the description of Cedratvirus and Orpheovirus, two new viruses related to Pithoviruses. Studying the major capsid protein of Orpheovirus, we detected a homologous sequence in a mine drainage metagenome. The in-depth exploration of this metagenome, using the MG-Digger program, enabled us to retrieve up to 10 contigs with clear evidence of viral sequences. Moreover, phylogenetic analyses further extended our screening with the discovery in another marine metagenome of a second virus closely related to Orpheovirus IHUMI-LCC2. This virus is a misidentified virus confused with and annotated as a Rickettsiales bacterium. It presents a partial genome size of about 170 kbp.

Giant viruses: update, enigmas, controversies and perspectives

Unlike microbes known in his time, the first virus (that of tobacco mosaic disease) was discovered by Ivanoski in 1892 because it was not retained by Chamberland's porcelain candles. For more than a century afterward, viruses were equated with this simple property that is still extensively used today (using modern 0,2 µm pore filters) as a practical criterion to delineate the "viral fraction" from other microbes in medical or environmental samples. The first documented exception to the simplistic criterion of particle size came with the discovery of Mimivirus, the viral nature of which was eventually recognized in 2003, following ten years during which it was mistaken for an obligate intracellular bacterium. Thirteen more years later, we now realize that non-filtering "giant viruses" are not rare, probably ubiquitous, and come in a large variety of virion shapes, genome sizes, gene contents, and replication strategies. Following a quick description of the 4 giant virus families known today, we discuss the enigmas, controversies and perspectives of conceptual revolutions that are brought about by this new and booming area of virology.

In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba

Acanthamoeba species are infected by the largest known DNA viruses. These include icosahedral Mimiviruses, amphora-shaped Pandoraviruses, and Pithovirus sibericum, the latter one isolated from 30,000-y-old permafrost. Mollivirus sibericum, a fourth type of giant virus, was isolated from the same permafrost sample. Its approximately spherical virion (0.6-µm diameter) encloses a 651-kb GC-rich genome encoding 523 proteins of which 64% are ORFans; 16% have their closest homolog in Pandoraviruses and 10% in Acanthamoeba castellanii probably through horizontal gene transfer. The Mollivirus nucleocytoplasmic replication cycle was analyzed using a combination of "omic" approaches that revealed how the virus highjacks its host machinery to actively replicate. Surprisingly, the host's ribosomal proteins are packaged in the virion. Metagenomic analysis of the permafrost sample uncovered the presence of both viruses, yet in very low amount. The fact that two different viruses retain their infectivity in prehistorical permafrost layers should be of concern in a context of global warming. Giant viruses' diversity remains to be fully explored.

Draft genome sequences of Terra1 and Terra2 viruses, new members of the family Mimiviridae isolated from soil

Since the discovery of Mimivirus, the founding member of the family Mimiviridae, three lineages, A–C, have been delineated among the mimiviruses of amoebae. To date, all giant viruses with annotated genomes have been isolated from water samples. Here, we describe the genome of two mimiviruses, Terra1 virus and Terra2 virus, which were recovered by co-culturing on Acanthamoeba spp. from soil samples. These genomes are predicted to harbor 1055 and 890 genes, respectively. Comparative genomics and phylogenomics show that Terra1 virus and Terra2 virus are classified within lineages C and A of the amoebae-associated mimiviruses, respectively. The genomic architecture of both viruses show conserved collinear central regions flanked by less conserved areas towards the extremities, when compared with other mimivirus genomes. A cluster of genes that are orthologous to bacterial genes and have no counterpart in other viral genomes except in lineage C mimiviruses was identified in Terra1 virus.

TRUC or the Need for a New Microbial Classification

Microbes were defined in the 19th century by L. Pasteur. Prokaryotes and eukaryotes, which are divided into two worlds of microbes, were introduced by E. Chatton in 1925. R. Woese divided this world into three domains based on ribosomal analysis (Bacteria, Archaea, and Eukarya). The discovery of Mimivirus and other Megavirales, that are microbes, led to divide the microbiological world into four branches. I introduced the name TRUC (Things Resisting Uncompleted Classifications) to accommodate the division in four of the currently known microbiological world.

Giant Viruses Revive Old Questions About Viral Origins

University of Aix-Marseille microbiologist Didier Raoult's 2004 discovery of Mimivirus —and several other giant viruses identified since then—has challenged the view that viruses are not alive.

Phylogenetic and Phyletic Studies of Informational Genes in Genomes Highlight Existence of a 4th Domain of Life Including Giant Viruses

The discovery of Mimivirus, with its very large genome content, made it possible to identify genes common to the three domains of life (Eukarya, Bacteria and Archaea) and to generate controversial phylogenomic trees congruent with that of ribosomal genes, branching Mimivirus at its root. Here we used sequences from metagenomic databases, Marseillevirus and three new viruses extending the Mimiviridae family to generate the phylogenetic trees of eight proteins involved in different steps of DNA processing. Compared to the three ribosomal defined domains, we report a single common origin for Nucleocytoplasmic Large DNA Viruses (NCLDV), DNA processing genes rooted between Archaea and Eukarya, with a topology congruent with that of the ribosomal tree. As for translation, we found in our new viruses, together with Mimivirus, five proteins rooted deeply in the eukaryotic clade. In addition, comparison of informational genes repertoire based on phyletic pattern analysis supports existence of a clade containing NCLDVs clearly distinct from that of Eukarya, Bacteria and Archaea. We hypothesize that the core genome of NCLDV is as ancient as the three currently accepted domains of life.