Lacquer painting in China is gaining recognition and growing rapidly. Many colleges and universities have set up the subject of lacquer painting, but there is no systematic understanding of how to set up the subject of lacquer painting. The author would like to find out the advantages and disadvantages of the study. Further, the research will provide and theoretical reference for the improvement of the subject construction of lacquer painting. This paper summarizes the formation, development and present situation of lacquer painting by means of literature research. Employing comparative analysis, on the construction of lacquer painting subject in several representative fine arts colleges and comprehensive universities, and finds out the characteristics of the construction of lacquer painting subject in colleges and universities in China. The author believes that the subject construction of lacquer painting should further optimize the teaching mode, strengthen the faculty and discipline management, in order to make the subject of lacquer painting in Chinese colleges and universities further develop.