Quantitative assessment of fatigue crack growth (FCG) in a Si-modified Inconel 939 alloy, processed by laser powder bed fusion additive manufacturing (L-PBF AM), was conducted through cyclic bending of pre-notched cantilevers with a characteristic cross-sectional dimension of 25 μm × 25 μm. To the best of our knowledge, this work is the first to show that cyclic bending of such pre-notched microscale cantilevers can sense both the threshold regime and the Paris-law regime of FCG quantitatively, despite the small specimen size. The deliberate fabrication of such microscale test specimens enables control of the direction of fatigue crack propagation with respect to a given microstructure, while avoiding the inclusion of volumetric defects within the test volume. Significant differences in FCG behavior were observed when the direction of crack propagation was switched from being parallel to the AM build direction to being perpendicular to it. The present work demonstrates the efficacy of FCG assessment using microscale specimens, which can enhance our understanding of the influence of a given microstructure on crack growth behavior.