Gaur (Bos gaurus) is listed as a vulnerable species in the IUCN Red List of threatened species due to the rapid population decline caused by human pressures in their habitats. To develop successful conservation plans, it is essential to understand the relationship between Gaur and their habitats. This study aimed to investigate the factors influencing Gaur habitat use and identify and rank conservation threats in Chitwan National Park, Nepal. Using line transect surveys, we recorded Gaur's presence via direct sightings and indirect signs (dung, footprints, horns) over an area of 176 km2 in July and August 2022. We used binary logistic regression models to determine the collected ecological and anthropogenic factors influencing the occurrence of Gaur and the relative whole-site threat ranking method to rank conservation threats. The results revealed that the probability of Gaur occurrence increases with moderate to high canopy cover, riverine and Shorea robusta dominated forests, and nearest distance to road/path/firelines, while decreasing with the presence of predators. Uncontrolled fire, invasive species, human disturbances, and climate change were ranked as the most prevailing threats to Gaur in our study area. Conservation managers should implement effective habitat management interventions, such as construction of waterhole, firelines maintenance, grassland management, and control of invasive species in the potential habitats, to safeguard and maintain the sustainability of Gaur populations and associated herbivores. Future studies should consider larger geographical settings and multiple seasons, and habitat suitability assessments should be conducted to determine current and future suitable habitat refugia for Gaur and other threatened wildlife species at the landscape level.