Multipath interference (MPI) noise induces drastic fluctuations in high-speed 4-level pulse amplitude modulation (PAM4) intensity modulation direct detection (IMDD) systems, severely degrading the transmission performance. Here, we propose a bias-aided decision-directed least mean square (DD-LMS) equalizer to eliminate the MPI noise. In the simulation, the proposed bias-aided DD-LMS equalizer could adeptly track and compensate the MPI-impaired PAM4 signal, markedly improving the bit error rate (BER) performance under different MPI levels and laser linewidths. Within a 112 Gbps PAM4 transmission system, the proposed equalizer achieves a MPI tolerance over 4 dB at the KP4-forward error correction (FEC) threshold (2.4 × 10−4), outperforming existing MPI suppression techniques.