In this paper, the investigated mixture of liquid crystals shows a spacious range of twist grain boundary phases (TGBPs). Thermo dynamical, optical and dielectric measurements confirmed the presence of TGBPs in the mixtures of nematic and cholesteric liquid crystals. In the heating cycle, differential scanning calorimetry (DSC) thermogram shows the mixed different TGBPs at the high scanning rate. Cylindrical and cone-like textures with brushes of TGBA* is observed under the optical investigation. The grain boundaries and blocks of smectic phases are twisting two times around an axis. Wide-ranging dielectric investigation of nematic and cholesteric liquid crystal mixture have been performed in the 50 Hz–10 MHz range of frequency for the homogeneous conditions. In the absence of direct bias, TGBA* phase exhibits two dielectric relaxation processes, soft mode (SM) and process in the grain boundaries (PGBs). In the presence of electric field, grain boundaries steadily demolished the phases. Whereas the weak dielectric relaxation processes have been distinguished in the presence of the electric field in the TGBPs.