This survey based descriptive research has been undertaken in Tirunelveli District, Tamil Nadu, as a comparative study to identify and differentiate sources of stress and its impact on morale of the nurses working in four core departments namely intensive care unit, operation theatre, emergency department and ward. Sources of stress have been identified under three dimensions namely homework-conflict-related stressors, doctor-related stressors and patient-related stressors. The study has sampled 360 nurses qualified with Diploma in General Nursing and Midwifery and the Bachelor of Science in Nursing courses from 45 general types of private hospitals using a stratified sampling technique. The weighted average method has been applied to analyse sources of stress. Mean, standard deviation and coefficient of variation have been used to understand the perception of nurses working in four core departments towards sources of stress. The Kruskal Wallis test has been administered to examine the relationship between sources of stress and demographic variables. Multiple regression models have been used to know relationship between sources of stress and morale. The result indicated that all three dimensions of stressors have been perceived at a medium level by the majority of the nurses. The result also found that among three dimensions of stressors home-work conflict-related stressors have occupied the first place followed by patient-related stressors and doctor-related stressors. The study has given suitable suggestions to overcome the stress and to improve morale of the nurses.