In the search for an extension of the ‘Bip method’ for determining the absolute configuration of β-amino acids and β-peptides, dipeptides based on β 2,2-HBip/ l( d)-Ala, Bip/ l-β 3-HAla, and β 2,2-HBip/ l-β 3-HAla were synthesized in solution and the induced circular dichroism (ICD) in their biphenyl core evaluated in comparison with the previously investigated Bip/ l( d)-Ala series. Weak, poorly informative ICDs were observed in MeOH solution for the linear N-Boc protected dipeptide methyl esters based on β 2,2-HBip, as well as for those with Ala/β 3-HAla at the N-terminus of Bip/β 2,2-HBip. However, a significant ICD was recorded for Boc-Bip- l-β 3-HAla-OMe. These results were confirmed by low-temperature 1H NMR spectroscopy studies of the dipeptides in CDCl 3 and CD 3OD solutions, showing two diastereoisomeric conformers in significantly different populations for Boc-Bip- l-β 3-HAla-OMe in CD 3OD. In general, ICDs were found to be weaker for dipeptides containing β-amino acids as compared to those of their α-amino acid counterparts.