Dioxin-like Polychlorinated Biphenyls Research Articles

Good eggs

Good eggs

Co-occurrence of contaminants in marine fish from the North East Atlantic Ocean: Implications for human risk assessment.

Marine fish from the North East Atlantic Ocean (NEAO) are nutrient rich and considered a valuable economic resource. However, marine fish are also a major dietary source of several contaminants, including persistent organic pollutants (POPs) and heavy metals. Using one of the world's largest seafood datasets (n>25,000 individuals), comprising 12 commercially important fish species collected during 2006-2019 in the NEAO, we assessed the co-occurrence of elements and POPs, and evaluated potential risks to human consumers. Several positive correlations between concentrations of mercury (Hg), dioxins, polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) were observed. Concentrations of Hg, dioxins, PCBs and PBDEs increased from North to South and associations between marine sediment contamination, sea temperature, and fish Hg and POPs concentrations were identified using multi-linear regression (MLR) models. In general, Hg concentrations in fillet and liver of fish were positively associated with increases in both sediment contamination and sea temperature. POPs concentrations in both fillet and liver were positively associated with increases in sediment contamination, and only POPs concentrations in the liver of benthopelagic and demersal species were found to be positively correlated with sea temperature. Using a probabilistic approach to estimate human contaminant exposure from seafood, we showed that intake of pelagic species posed the highest risk of dioxins and dioxin-like PCBs (DL-PCBs) exposure, while intake of benthopelagic and demersal species posed the highest risk of Hg exposure. This study can serve as a model to further understand the distribution, co-occurrence, and trends of contaminants in seafood harvested from the NEAO and their potential risks to human consumers.

Forty years studies on polychlorinated biphenyls pollution, food safety, health risk, and human health in an e-waste recycling area from Taizhou city, China: a review.

E-waste generation has become a serious environmental challenge worldwide. Taizhou of Zhejiang Province, situated on the southeast coastline of China, has been one of the major e-waste dismantling areas in China for the last 40years. In this review, we focused on the polychlorinated biphenyl (PCB) trends in environmental compartments, burden and impact to humans, food safety, and health risk assessment from Taizhou, China. The review suggested that PCBs showed dynamic trends in air, soil, water, biodiversity, and sediments. Soils and fish samples indicated higher levels of PCBs than sediments, air, water, and food items. PCB levels decreased in soils with the passage of time. Agriculture soils near the e-waste recycling sites showed more levels of total PCBs than industrial soils and urban soils. Dioxin-like PCB levels were higher in humans near Taizhou, suggesting that e-waste pollution could influence humans. Compared with large-scale plants, simple household workshops contributed more pollution of PCBs to the environment. Pollution index, hazard quotient, and daily intake were higher for PCBs, suggesting Taizhou should be given priority to manage the e-waste pollution. The elevated body burden may have health implications for the next generation. The areas with stricter control measures, strengthened laws and regulations, and more environmentally friendly techniques indicated reduced levels of PCBs. For environment protection and health safety, proper e-waste dismantling techniques, environmentally sound management, awareness, and regular monitoring are very important.

Human health risk assessment for PAHs, phthalates, elements, PCDD/Fs, and DL-PCBs in PM2.5 and for NMVOCs in two East-Mediterranean urban sites under industrial influence

This study evaluates the carcinogenic and the non-carcinogenic health risks related to non-methane volatile organic compounds (NMVOCs) and elements, dioxins, furans, dioxin-like polychlorobiphenyls, phthalates and polycyclic aromatic hydrocarbons in PM2.5 samples collected during a one-year field campaign in two urban industrial areas in the East Mediterranean region. The health risk was assessed for the three exposure pathways (ingestion, inhalation and dermal contact) and for different age categories (newborns, children, adolescents, and adults). The non-carcinogenic risk calculated for the different species showed that benzene and n-heptane explained 78–94% of the total hazard index (HI) for NMVOCs at both sites. The total HI for NMVOCs varied between 2.9 and 26.8 at Zouk and between 0.8 and 6.6 at Fiaa for adults and newborns respectively exceeding the recommended USEPA limit of 1 for most age categories. PM2.5-bound elements had values higher than the recommended USEPA limit for newborns with Mn, Pb, V, and Ni as the major contributors. The other species under study presented moderate risk values. The lifetime cancer risk due only to the exposure to NMVOCs was 170 and 46 times higher than the threshold limit at Zouk and Fiaa, respectively. PM2.5-bound PAHs, As, Co, Cr(VI), Ni and V concentrations showed lifetime cancer risk exceeding the threshold limit of 10−6 with 58 and 28 additional cancer cases per million habitats at Zouk and Fiaa, respectively. To our knowledge, this assessment is a first evaluating the health risk of several classes of compounds from both particulate and gas phases.

Evaluation of Extraction Procedure of PCDD/Fs, PCBs and Chlorobenzenes from Activated Carbon Fibers (ACFs).

Active carbon-based sorbents are well known and are used in analytical chemistry. Activated carbon fibers (ACFs) are mainly used as abatement systems in industrial emission pollution control. The objective of this study was to extend the use of ACFs in analytical chemistry for the analysis of polychlorodibenzo-p-dioxins (PCDDs), polychlorodibenzofurans (PCDFs), dioxin-like polychlorobiphenyls (PCBs), and chlorobenzenes (CBs). For this purpose, the extraction efficiency was evaluated based on the QA/QC criteria defined by EPA/ISO reference methods on 13C-standards recovery rates. The procedures tested were ultrasonic assisted extraction (UAE), Soxhlet extraction (SE), accelerated solvent extraction (ASE), and microwave-assisted extraction (MAE). Each experiment was performed in triplicate to ensure the repeatability of the results, and a second extraction assessed the complete extraction. The comparison of the results of each set of experiments with the minimum requirements of the reference methods for each class of compounds led to SE being chosen as the best technique. SE with toluene resulted in a reduction of time and costs and with respect to the other investigated techniques. The present work demonstrated that ACFs can be used in environmental fields means of both prevention and control (exploiting the adsorbent characteristics) and for analytical purposes (exploiting the desorption) for the described chlorinated classes of pollutants.

Associations of paternal serum dioxin-like polychlorinated biphenyl concentrations with IVF success: A pilot study

Dioxin-like polychlorinated biphenyls (DL-PCBs) are environmental pollutants that have been associated with impaired semen quality. However, research on the potential impact of paternal exposure to DL-PCBs and the risk of adverse pregnancy outcomes are limited. We examine the relationship between serum DL-PCB concentrations and IVF outcomes among 42 males seeking fertility treatment. Concentrations of 12 serum DL-PCBs were analyzed by high-resolution gas chromatography coupled to high-resolution mass spectrometry. Modified Poisson regressions, adjusted for confounders, were used to assess bivariate associations and to estimate risk ratios (RRs) between DL-PCBs and binary IVF outcomes. The median concentration (25th-75th percentiles) of the sum of the 12 DL-PCBs (∑DL-PCBs) obtained for the patients was 5.42 (3.78–7.78) ng/g lipid. No statistically significant association between DL-PCB levels and embryo quality was found. However, men with high serum PCB-77 concentrations present more probability of high-quality embryos (RR: 0.292; 95% CI: 0.090–0.942), whereas the opposite trend is observed for men with lower serum levels of PCB-156 (RR: 7.960; 95% CI: 1.020–62.100), who present increased odds of high-quality embryos. Serum concentrations of PCB-126 and PCB-114 were associated with decreased implantation rates (p < 0.05). Moreover, PCB-77 and ∑non-ortho PCBs were significantly associated with a lower likelihood of clinical pregnancy (p < 0.05). A lower likelihood of live birth was associated with higher levels of PCB-77, PCB-105, PCB-118, and recording significant differences for ∑non-ortho PCBs, ∑mono-ortho PCBs, and ∑DL-PCBs (p < 0.05).These findings suggest that paternal DL-PCB exposure before conception may be related to pregnancy endpoints. However, DL-PCB measurement were limited to male partners. Therefore, we propose that future studies with larger population sizes should include both maternal and paternal factors.

Extraction of Chlorobenzenes and PCBs from Water by ZnO Nanoparticles

Metal oxide nanoparticles have great potential for selective adsorption and catalytic degradation of contaminants from aqueous solutions. In this study, we employ mass spectrometry and molecular dynamics simulations to better understand the chemical and physical mechanisms determining the affinity of chlorobenzenes and polychlorinated biphenyls (PCBs) for zinc oxide nanoparticles (ZnO NPs). The experiments and simulations both demonstrate that the adsorption coefficients for chlorobenzenes increase steadily with the number of chlorine atoms, while, for PCBs, the relation is more complex. The simulations link this complexity to chlorine atoms at ortho positions hindering coplanar conformations. For a given number of chlorine atoms, the simulations predict decreasing adsorption affinity with increasing numbers of ortho substitutions. Consequently, the simulations predict that some of the highest adsorption affinities for ZnO NPs are exhibited by dioxin-like PCBs, suggesting the possibility of selective sequestration of these most acutely toxic PCBs. Remarkably, the experiments show that the PCB adsorption coefficients of ZnO NPs with diameters ≤ 80 nm exceed those of a soil sample by 5–7 orders of magnitude, meaning that a single gram of ZnO NPs could sequester low levels of PCB contamination from as much as a ton of soil.

Distribution, Source and Potential Risk Assessment of Polychlorinated Biphenyls (PCBs) in Sediments from the Liaohe River Protected Area, China

This study aimed to assess the occurrence, distribution, toxicity equivalency and health risks of dl-PCBs (dioxin-like PCBs) from nine sites collected in surface sediments from Liaohe River Protected Area. ∑dl-PCBs concentrations in sediments range from 79.2 to 365.1 pg/g. Sediment profiles showed that pentachlorobiphenyl is the most abundant congener among all sampling sites. The results of principal component analysis and cluster analysis indicated that PCBs were mainly derived from electronic waste and paint additives in the sediments of Liaohe River Protected Area. Toxic equivalent quantity (TEQ) values of the PCBs in the Liaohe River Protected Area sediments are at comparatively lower levels compared with the previously reported data. Hazardous ratio (HR) for human health risk assessment allied to cancer was found to be lower than the non-carcinogenic risk assessment within an acceptable range.

Human health risk assessment of the levels of dioxin-like polychlorinated biphenyls (PCBs) in soils from mechanic workshops within Nekede mechanic village, Imo State, Nigeria

ABSTRACT Dioxin-like polychlorinated biphenyls (DL-PCBs) are a class of persistent toxic substances with a high potential to accumulate in the soil as an organic pollutant and bioaccumulate in humans. The study determined the level and human health risk of twelve (12) DL-PCBs in soils from mechanic workshops within Nekede mechanic village (NMV), Imo State. In this study, soil samples (0–15 cm depth) were collected at four different sampling points within Nekede mechanic village. At each sampling point, composite samples consisting of 10 sub-samples were collected with pre-cleaned pet bottles using soil auger and were labelled SA, SB, SC, SD. The control sample was taken 500 metres away from the mechanic village where there was no form of auto-mechanic activities. Sample analysis was conducted using Agilent GC 6890 N coupled with Agilent MS 5970B to determine the levels of DL-PCBs. The data were analysed using SPSS version 23. The results revealed that the mean concentration of 12 DL-PCB congeners (Σ12PCBs) in soil ranged from 0.31–16.31 µg kg−1, which was significantly higher than that in the control sample (0.006–0.087 µg kg−1). PCB-126 was the major contributor (58.99%) while the least contributor was PCB-157 (0.0040%) of the Σ12 DL-PCBs TEQ. The sum total LCR (adults 1.29 × 101, children 4.42 × 101) and HQs (adults 6.68 × 102, children 2.29 × 103) were above the acceptable risk limit (LCR = 10−4, HQ = 1); indicating a very high adverse effect of DL-PCBs on humans especially children.

Monitoring temporal trends of dioxins, organochlorine pesticides and chlorinated paraffins in pooled serum samples collected from Northern Norwegian women: The MISA cohort study

The ubiquitous presence of legacy and emerging persistent organic pollutants (POPs) in the environmental matrices poses a potential hazard to the humans and creating public health concerns. The present study aimed to evaluate dioxins, dioxin-like polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and chlorinated paraffins (CPs) concentrations in serum of women (postpartum, pregnant and non-pregnant) from Northern Norway to better understand their exposure and contamination status as well as temporal trends across 2007–2009 (MISA 1) to 2019 (MISA 2). Sixty-two blood samples from the MISA 1 cohort and 38 samples from MISA 2 were randomly selected in this study (n = 100). Ninety samples from postpartum (MISA 1) and pregnant women (MISA 2) were randomly combined into 9 pools, with 9–11 individual samples contributing to each pool keeping the groups of pregnant and postpartum women. Remaining 10 samples from non-pregnant women (MISA 2) were allocated into separate group. Geometric mean, minimum and maximum were used to describe the serum concentrations of pooled POPs in MISA cohort. Mann-Whitney U test and independent sample t-test were applied for trend analysis of blood levels of POPs between MISA 1 and MISA 2. We found the serum concentrations of selected POPs in this study to be at lower range. Serum concentrations of dibenzo-p-dioxins (PCDDs) (p = 0.010), polychlorinated dibenzofurans (PCDFs) (p = 0.002), dioxins-like PCBs (p = 0.001), hexachlorobenzene (HCB) (p < 0.001) and p,p’-dichlorodiphenyldichloroethylene (p,p’-DDE) (p = 0.002) were decreased between the studied time. In contrast, the serum concentrations of medium chain chlorinated paraffins showed an increasing trend between 2007 and 2009 and 2019 (p = 0.019). Our findings report a particular concern of emerging contaminant medium chain chlorinated paraffin exposure to humans. Future observational studies with repeated measurements of chlorinated paraffins in general populations worldwide and large sample size are warranted.

Intrauterine exposure to 2,3′,4,4′,5-pentachlorobiphenyl alters spermatogenesis and testicular DNA methylation levels in F1 male mice

Polychlorinated biphenyls (PCBs) are synthetic biphenyl compounds with high toxicity. There are a total of 209 homologs, among which 2,3’,4,4’,5-pentachlorobiphenyl (PCB118) is one of the dioxin-like PCBs. PCB118 can accumulate in pregnant mice, leading to fetus directly exposure during development. The stage of migration of mouse primordial germ cells ranges from 8.5 to 13.5 days of pregnancy, which is the stage undergoing a genome-wide DNA demethylation process. In this study, the mice were exposed to 20 μg/kg/day and 100 μg/kg/day PCB118 from 8.5 to 13.5 days of pregnancy. During the embryo stage at 18.5 days (E18.5 days), the expression level of DNA methyltransferase 1 (Dnmt1) was reduced in the testes, and the DNA methylation level in mouse testes were also decreased. We found that the seminiferous tubules showed vacuolization and that the sperm deformity rate increased in the treated groups compared with the control group in 7-week-old mice. Because exposure to PCB118 during pregnancy causes damage to the reproductive system of male offspring mice, attention should be devoted to the toxicity transmission of persistent environmental pollutants such as PCBs.

The Associations of Prenatal Exposure to Dioxins and Dioxin-Like Polychlorinated Biphenyls with Neurodevelopment at 6 Months of Age: Multi-pollutant Approaches

BACKGROUND AND AIM: Prenatal exposure to endocrine disrupting chemicals, including polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (DL-PCBs), has been hypothesized to have detrimental impact on neurodevelopment. However, the effect of prenatal exposure to dioxin and DL-PCB mixtures on neurodevelopment remains largely inconclusive. Therefore, we aimed to elucidate the associations of in utero exposure to a dioxin and DL-PCB mixture with infant neurodevelopment by applying novel multi-pollutant methods. METHODS: The concentrations of individual dioxin and DL-PCB isomers were measured in maternal peripheral blood during pregnancy in the Sapporo cohort, Hokkaido Study on Environment and Children’s Health. The mental and psychomotor development of the study participants’ six-month-old infants were assessed using the Bayley Scales of Infant Development-2nd Edition (n=210). To determine both the joint and individual associations of prenatal exposure to dioxin and DL-PCB mixtures with infant neurodevelopment, Bayesian Kernel Machine Regression (BKMR) and quantile-based g-computation models were performed. RESULTS:Suggestive, but not significant, inverse associations were observed between prenatal exposure to a dioxin and DL-PCB mixture, and infant psychomotor development in the both BKMR and quantile g-computation models. In contrast, we found no association of dioxin and DL-PCB mixtures with mental development. These mixture methods consistently indicated 2,3,7,8-TCDD and 23'44'55'-HxCB(#167) as the most influential dioxin and DL-PCB congener on mental and psychomotor development, respectively. When group-specific posterior inclusion probabilities were evaluated, BKMR revealed in utero exposure to PCDFs and mono-ortho DL-PCBs as the more important contributing factors to early mental and psychomotor development, respectively, compared with the other dioxin or DL-PCB groups. No evidence of non-linear relationships or interactions among the chemical mixtures was detected. CONCLUSIONS:Employing the novel statistical methods for chemical mixture analysis, we demonstrated limited evidence of inverse associations of prenatal exposure to dioxins and DL-PCBs with infant psychomotor development, but not with mental development. KEYWORDS: Chemical mixtures, Dioxins, Polychlorinated biphenyls, Neurological development, Bayesian Kernel Machine Regression, Quantile g-computation

Reevaluation on accumulation and depletion of dioxin-like compounds in eggs of laying hens: Quantification on dietary risk from feed to egg

Updated assessment on transfer of polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) from feed to food is essential for understanding human exposure risk. A controlled feeding experiment was conducted for laying hens to reevaluate the transfer characteristics of dioxin-like compounds from feed to egg. Two fortified diets (1.17 and 5.13 pg TEQ g−1 dry weight), made by blending with fly ash, were fed to laying hens for 14 days, followed by 28-days depletion. Levels of ∑TEQPCDD/Fs+DL-PCBs in eggs rapidly increased once exposure started, reaching at 1.98 and 6.86 pg TEQ g−1 lw at the end of exposure for low- and high-exposure groups, respectively, and dropped to under the European legislation (maximum levels: 5.0 pg TEQ g−1 lw) after 28-days depletion. The quantitative depletions showed that the half-lives (T1/2) of ∑TEQPCDD/Fs in eggs were 23 and 14 days for low- and high-exposure groups, respectively. The depletion and accumulation rates of PCDD/Fs were in the range of 0.026–0.151 and 0.005–0.016 day−1, respectively, representing that the T1/2 of PCDD/Fs in eggs ranged from 5 to 27 days. The depletion kinetics of DL-PCBs was not significant in egg. The hens with higher laying rates exhibited shorter T1/2 of PCDD/Fs, implying that increasing laying rate could expedite the depletion of PCDD/Fs in egg. The T1/2 of PCDD/Fs in egg were negatively correlated with the chlorine number, indicating that lower chlorinated congeners tended to be retained in the egg. Transfer rates of PCDD/Fs were in the range of 4–19%, which were lower than the previous results. These results were attributed to short exposure time and low bioavailability of PCDD/Fs in fly ash. Estimations of dietary intake highlighted the dietary risk of PCDD/Fs from feed to egg, which would pose limited adverse effects on human health.

Occurrence and risks of PCDD/Fs and PCBs in three raptors from North China

Concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) were investigated in muscle samples from common kestrels (Falco tinnunculus), eagle owls (Bubo bubo), and little owls (Athene noctua) collected in Beijing, China. The concentrations of PCDD/Fs were in the ranges of 22.7–5280, 67.5–1610, and 68.4–3180 pg/g lipid weight (lw), while levels of dioxin-like PCBs ranged from 4.91 to 1560, 8.08–294, and 28.2–3540 ng/g lw, in common kestrel, eagle owl, and little owl, respectively. The main PCDD/Fs congener was 2,3,4,7,8-PeCDF, and CB-153 dominated the seven indicator PCBs. PCB levels have shown a decreasing trend in the last decade for the common kestrel, but not for little owl in Beijing, which exhibited higher levels of pollutants and toxic equivalency (TEQ) values than the other two species. Concentrations of PCDD/Fs, dioxin-like PCBs, and indicator PCBs differed between fledgling and adult raptors for certain species. Raptors in this study generally had a higher TEQ than the no-observed-effect level in the literature, indicating significant exposure risks to PCDD/Fs and dioxin-like PCBs in raptors, especially in adult little owls.

The Aryl hydrocarbon receptor mediates reproductive toxicity of polychlorinated biphenyl congener 126 in rats

Polychlorinated biphenyls (PCBs) are endocrine disrupting chemicals with documented, though mechanistically ill-defined, reproductive toxicity. The toxicity of dioxin-like PCBs, such as PCB126, is mediated via the aryl hydrocarbon receptor (AHR) in non-ovarian tissues. The goal of this study was to examine the uterine and ovarian effects of PCB126 and test the hypothesis that the AHR is required for PCB126-induced reproductive toxicity. Female Holzman-Sprague Dawley wild type (n = 14; WT) and Ahr knock out (n = 11; AHR−/−) rats received a single intraperitoneal injection of either corn oil vehicle (5 ml/kg: WT_O and AHR−/−_O) or PCB126 (1.63 mg/kg in corn oil: WT_PCB and AHR−/−_PCB) at four weeks of age. The estrous cycle was synchronized and ovary and uterus were collected 28 days after exposure. In WT rats, PCB126 exposure reduced (P < 0.05) body and ovary weight, uterine gland number, uterine area, progesterone, 17β-estradiol and anti-Müllerian hormone level, secondary and antral follicle and corpora lutea number but follicle stimulating hormone level increased (P < 0.05). In AHR−/− rats, PCB126 exposure increased (P ≤ 0.05) circulating luteinizing hormone level. Ovarian or uterine mRNA abundance of biotransformation, and inflammation genes were altered (P < 0.05) in WT rats due to PCB126 exposure. In AHR−/− rats, the transcriptional effects of PCB126 were restricted to reductions (P < 0.05) in three inflammatory genes. These findings support a functional role for AHR in the female reproductive tract, illustrate AHR's requirement in PCB126-induced reprotoxicity, and highlight the potential risk of dioxin-like compounds on female reproduction.

Soil concentrations, compositional profiles, sources and bioavailability of polychlorinated dibenzo dioxins/furans, polychlorinated biphenyls and polycyclic aromatic hydrocarbons in open municipal dumpsites of Chennai city, India.

Several studies have reported the release of halogenated persistent organic pollutants (POPs) and polycyclic aromatic hydrocarbons (PAHs) associated with open burning of municipal solid waste. Considering soil as a sink for such organic contaminants, we conducted an in-depth study on the surface soil concentrations of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs), polychlorinated biphenyls (PCBs) and sixteen USEPA enlisted PAHs collected from thirteen zones of the two major municipal dumpsites, Kodungaiyur dumpsite (KDS) and Perungudi dumpsite (PDS) of Chennai city. Indigenous microbes from dumpsite soil samples were isolated and identified based on 16S rRNA sequencing and phylogenetic analysis. Using indigenous microbes, we have elucidated the bioavailability of the targeted organic pollutants for each site.Range of Σ17PCDD/Fs, Σ25PCBs and ∑16PAHs varied between 3.96-612pg/g (96.0pg/g; median), ND-182ng/g (6.35ng/g; median) and 0.62-3649ng/g (64.3ng/g; median), respectively. All the dumpsite samples showed bioavailability for POPs and PAHs. Toxicity equivalent values (TEQs) associated with dioxin-like PCBs and PCDD/Fs from the zones where dumped municipal solid wastes were collected from electronic-waste/IT-corridor/port areas and toxic PAHs from the zone receiving wastes from the industrial corridor of the city were higher than the soil permissible limit prescribed by the World Health Organization.

Dioxins and dioxin-like PCBs in buffalo milk from the Campania region (Italy): Decreasing trend and baseline assessment over 10 years (2008–2018)

Polychlorodibenzo-p-dioxins, polychlorodibenzo-furans (PCDD/Fs) and dioxin-like polychlorobiphenyls (DL-PCBs) enter the food chain from the environment. In this study, we report the 2008–2018 time-trends in the PCDD/F and DL-PCB contamination of milk from buffaloes fed on local forage in rural areas of the Campania region. Validated according to QA/QC criteria, the dataset (N = 808 on a total of 2068 samples, after excluding follow-up results and outliers) was computed on the upper-bound value pg WHO-TEQ2005 g−1fat. We assessed time-trends and assayed baseline contamination levels, which displayed log-normal distribution. A significant decreasing trend (p < 0.01) was observed from 2008 to 2009 and 2010; the P50–P95 range fell from 2.37–8.48 pg WHO-TEQ2005 g−1fat (N = 393) in 2008 to 1.73–4.61 in 2009 (N = 86) and to 0.67–1.46 in 2010 (N = 42). From 2010 to 2018 (N = 329), no significant variation was found among years and the related dataset fitted a log-normal distribution (p < 0.05). Occurrence descriptors indicated that the baseline contamination of dairy products (mean = 0.54; P50–P95 = 0.47–1.24) in the Campania Region was well below the EU regulatory limit in force (5.5 pg WHO-TEQ2005 g−1fat). Given the Tolerable Weekly Intake of 2 pg WHO-TEQ kg−1 body weight (bw) proposed by the EFSA for PCDD/Fs and DL-PCBs, this level of baseline contamination is discussed with regard to the orientation of food safety and food security risk connected with buffalo mozzarella cheese production.

Effects of iron-carbon materials on microbial-catalyzed reductive dechlorination of polychlorinated biphenyls in Taihu Lake sediment microcosms: Enhanced chlorine removal, detoxification and shifts of microbial community

Nano zero-valent iron particles (nZVI, 0.09 wt%), micro zero-valent iron particles (mZVI, 0.09 wt%), granular activated carbon (GAC, 3.03 wt%), GAC supported nZVI (nZVI/GAC, 3.12 wt%) and nZVI&GAC (nZVI 0.09 wt%, GAC 3.03 wt%) were evaluated for their effects on polychlorinated biphenyls (PCBs) anaerobic reductive dechlorination, detoxification, as well as microbial community structure in Taihu Lake (China) sediment microcosms. The results showed that all of these five materials could stimulate PCBs reductive dechlorination, especially for dioxin-like PCB congeners, and nZVI&GAC had the best removal effect on PCBs. The reduction of total PCBs increased from 13.5% to 33.2%. H2 generated by zero-valent iron corrosion was utilized by organohalide-respiring bacteria (OHRB) to enhance the dechlorination of PCBs predominantly via meta chlorine removal in the short term. The addition of ZVI had little impact on the total bacterial abundance and the microbial community structure. The adsorption of GAC and potential bioremediation properties of attached biofilm could promote the long-term removal of PCBs. GAC, nZVI/GAC, nZVI&GAC had different influences on the microbial structure. These findings provide insights into the biostimulation technique for in situ remediations of PCBs contaminated sediments.

Effects of curcumin on the bioavailability of dioxin-like pollutants in rats

The effects of curcumin on the bioavailability of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) were investigated in Sprague–Dawley rats. Tetra- and penta-chlorinated PCDFs had the lowest bioavailability and hexa-chlorinated PCDD/Fs had the highest, while there was no obvious change in that of DL-PCBs. Curcumin markedly reduced the toxic equivalent (TEQ) of PCDD/Fs in rats, illustrating the potential to competitively inhibit absorption of PCDD/Fs by the epithelial cells of the small intestine due to the similar chemical structure (diphenyl) between curcumin and PCDD/Fs. Moreover, curcumin lowered the TEQ of DL-PCBs in the liver of male rats, but not female rats. The significant decrease in the bioavailability of PCDD/Fs and DL-PCBs demonstrates the potential detoxification mechanisms of curcumin.

Passive Air Sampling of PCDD/Fs, PCBs, PAEs, DEHA, and PAHs from Informal Electronic Waste Recycling and Allied Sectors in Indian Megacities.

Xenobiotic chemical emissions from the informal electronic waste recycling (EW) sector are emerging problem for developing countries, with scale and impacts that are yet to be evaluated. We report an intensive polyurethane foam disk passive air sampling study in four megacities in India to investigate atmospheric organic pollutants along five transects viz., EW, information technology (IT), industrial, residential, and dumpsites. Intraurban emission sources were estimated and attributed by trajectory modeling and positive matrix factorization (PMF). ∑17PCDD/Fs, ∑25PCBs, ∑7plasticizers, and ∑15PAHs concentrations ranged from 3.1 to 26 pg/m3 (14 ± 7; Avg ± SD), 0.5-52 ng/m3 (9 ± 12); 7.5-520 ng/m3, (63 ± 107) and 6-33 ng/m3 (17 ± 6), respectively. EW contributed 45% of total PCB concentrations in this study and was evidenced as a major factor by PMF. The dominance of dioxin-like PCBs (dl-PCBs), particularly PCB-126, reflects combustion as the possible primary emission source. PCDD/Fs, PCBs and plasticizers were consistently highest at EW transect, while PAHs were maximum in industrial transect followed by EW. Concentrations of marker plasticizers (DnBP and DEHP) released during EW activities were significantly higher (p < 0.05) in Bangalore than in other cities. Toxic equivalents (TEQs) due to dl-PCBs was maximum in the EW transect and PCB-126 was the major contributor. For both youth and adult, the highest estimated inhalation risks for dl-PCBs and plasticizers were seen at the EW transect in Bangalore, followed by Chennai and New Delhi.