Dionex IonPac Research Articles

Rapid analysis of trace pollutants chlorates, perchlorates, and four xanthates in water based on IC-MS combined technology

Water, as the source of life, plays a crucial role in life activities. It also acts as a solvent, allowing toxic and harmful substances to migrate through the water environment. In this study, an analytical method based on ion chromatography-mass spectrometry (IC-MS) is established for the direct determination of trace chlorates, perchlorates, and four xanthates in water. The Dionex IonPac AS20 chromatography column is used as the analytical column, and the CR-TC device automatically generates KOH eluent and sets up a multi-step gradient program for elution. The Dionex ADRS 600 self-circulating regeneration ion suppressor (2 mm) generates H+ through electrolysis of water, replacing metal ions in the sample solution and eluent, enabling direct tandem ion chromatography and mass spectrometry. The water sample is directly measured by IC-MS after being filtered by a hydrophilic polytetrafluoroethylene (PTFE) filter head. The results indicate that chlorate and perchlorate show good linearity in the range of 0.1–100 ng mL−1, with a quantification limit of 0.3 μg L−1. The four xanthates exhibit a good linear relationship in the range of 0.5–500 ng mL−1, and the quantification limit can be reduced to 1 μg L−1. The method offers advantages such as high recovery rate, good stability, and strong reproducibility. It shows high consistency with the results detected by the LC-MS method, but with the added benefits of being more advanced and time-saving. This innovative technology provides a new detection method for trace pollutants in water, which can aid in the prevention and control of pollutants in the water environment.

Universal ion chromatography method for anions in active pharmaceutical ingredients enabled by computer-assisted separation modeling

Ion Chromatography (IC) is one of the most widely used methods for analyzing ionic species in pharmaceutical samples. A universal IC method that can separate a wide range of different analytes is highly desired as it can save a lot of time for method development and validation processes. Herein we report the development of a universal method for anions in active pharmaceutical ingredients (APIs) using computer-assisted chromatography modeling tools. We have screened three different IC columns (Dionex IonPac AS28-Fast 4 µm, AS19 4 µm and AS11-HC 4 µm) to determine the best suitable column for universal IC method development. A universal IC method was then developed using an AS11-HC 4 µm column to separate 31 most common anionic substances in 36 mins. This method was optimized using LC Simulator and a model which precisely predicts the retention behavior of 31 anions was established. This model demonstrated an excellent match between predicted and experimental analyte retention time (R2 =0.999). To validate this universal IC method, we have studied the stability of sulfite and sulfide analytes in ambient conditions. The method was then validated for a subset of 29 anions using water and organic solvent/water binary solvents as diluents for commercial APIs. This universal IC method provides an efficient and simple way to separate and analyze common anions in APIs. In addition, the method development process combined with LC simulator modeling can be effectively used as a starting point during method development for other ions beyond those investigated in this study.

Trypsin Digestion Pretreatment of Kelp Samples and the Iodine Speciation Analysis of Iodate, Iodide, MIT, and DIT by HPLC-ICP-MS

The analysis of iodine content in kelp is mainly focused on determining total iodine content, which lacks a rapid and sensitive analytical method for iodine species corresponding to iodate, iodide, monoiodotyrosine (MIT), and diiodo-tyrosine (DIT). Based on exploring the pretreatment methods of enzymatic hydrolysis of kelp, a rapid determination method of high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for different iodine species existed in kelp samples was established. The pretreatment method is trypsin hydrolysis of kelp samples treated at 50°C for 10 hours, then the extraction solution was obtained by centrifugation, and filtration was proved for the first time. A high-efficiency separation anion-exchange column (Dionex IonPac AS-14) for iodate, iodide, MIT, and DIT with 0.1 mol·L−1(NH4)2CO3 solution at pH = 9 as a mobile phase, a flow rate of 0.8 mL·min−1 for the first 1500 s, and 1.2 mL·min−1 for the last 1800 s was confirmed. The linear ranges of the standard calibrations on four iodine species are 1.0–150 μg·L−1, and the detection limits are 1.62, 1.02, 1.59, and 2.55 μg·L−1, respectively, while the RSD values of four iodine species are all less than 2%. Under the optimized conditions, the proposed method was successfully applied for the speciation analysis of iodine in kelp samples.

Germanium speciation study in soil from an electronic waste processing plant area

PurposeGermanium (Ge) is known as a Technology Critical Element (TCE) and has been widely used in electronic and industrial products. Waste electronic and electrical equipment (WEEE) containing germanium goes to WEEE treatment plants. The transfer of Ge in the environment makes it necessary to get to know its mobility, reactivity, and chemical transformations in soil. The objective of this research was to develop a methodology for germanium speciation in soil under pressure from storage, processing, and recovery from electrowaste.Materials and methodsThirty soil cores (30 cm long) were collected from an area around a WEEE plant, using a Humax soil sampler. Soil magnetometry methods were used for the first time during the determination of soil sampling points for germanium. After basic physicochemical analysis (pH, Eh, total element concentration using the ICP-MS technique), soil samples were prepared for germanium speciation. The optimisation and validation of a methodology for determining Ge species in easily-leached fractions of soil collected from areas around an electrowaste plant, was studied using Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry (IC-ICP-MS). Chromatographic conditions were optimised, taking soil matrix effects into account. Optimised Ge extraction from the soil included: extraction time, type of extractants, extraction support using shaking, and ultrasound.Results and discussionThe Ge species (Ge(IV), monomethylgermanium (MMGe), and dimethylgermanium (DMGe)) were successfully separated after 12 min on a Dionex IonPac AS9-HC column with ammonium nitrate and potassium sodium tartrate as an elution phase. The highest extraction efficiency was achieved by using 100 mM NH4NO3 with 1 mM potassium sodium tartrate as an extractant, that washes out Ge from soils within 4 h. In most cases, the soils contained a Ge(IV) form. Germanium methyl derivatives were only found in a few soil samples. Our research showed that germanium concentration in the studied area was as high as 7.64 mg.kg−1, with an average concentration of 3.51 mg.kg−1. The results allowed the creation of a correlation matrix, which identified many strong correlations.ConclusionThis research confirmed the impact of a WEEE plant on the increase of the Ge content in topsoil, when in direct proximity. Increased germanium content occurred in soil samples located north of the emitter, which is consistent with the prevailing wind direction. The study confirmed that the soil magnetometry method is applicable for detecting TCE. Soil samples with the highest content of germanium had a very high magnetic susceptibility and there is a correlation between these parameters.

Determination of 10 organic acids in alcoholic products by ion chromatography-tandem mass spectrometry

A method was developed for the determination of 10 organic acids in liquor, yellow rice wine, and dry red wine by ion chromatography-triple quadrupole mass spectrometry (IC-MS/MS). First, the liquor samples were diluted with deionized water, degassed with nitrogen, and analyzed by IC-MS/MS. Then, the yellow rice wine and dry red wine samples were purified with different solid-phase extraction cartridges. Finally, the GCB solid-phase extraction cartridge was selected for purification, diluted with deionized water, and analyzed by IC-MS/MS. The samples were separated using a Dionex IonPac AS11-HC anion analysis column with high capacity and strong hydrophilicity, with an KOH aqueous solution as the eluent, which was produced by an automatic generator for gradient elution. After being suppressed using a suppressor, the eluent was injected directly into the electrospray ionization tandem mass spectrometry (ESI-MS/MS), ionized in negative ion mode, detected in multiple reaction monitoring (MRM) mode, and quantified using an external standard method. Oxalic acid, fumaric acid, maleic acid, malic acid, tartaric acid, citric acid, quinic acid, and aconitic acid showed good linear relationships in the range of 0.05-2 mg/L. Succinic acid and lactic acid showed good linearities in the range of 0.05-5 mg/L and 0.05-10 mg/L, respectively. The correlation coefficients (r2) were >0.99. The limits of detection (LODs) and limits of quantification (LOQs) were 1.0-8.0 μg/L and 3.5-26.5 μg/L, respectively. The average recoveries ranged from 83.0% to 112.1%, and the relative standard deviations (RSDs) were <9.1% in spiked samples at three levels. The proposed method allowed easy pretreatment without using organic solvents or derivatization processing. Overall, the proposed method is accurate, rapid, sensitive, and it is suitable for the qualitative and quantitative analyses of the 10 organic acids in three wine samples. Moreover, it can be used for the determination of flavor and quality of alcoholic products.

Spin-Enhanced C–C Coupling in CO 2 Electroreduction with Oxide-Derived Copper

Spin-Enhanced C–C Coupling in CO ₂ Electroreduction with Oxide-Derived Copper

Optimization of Isocratic Ion Chromatography Methods for Simultaneous Inorganic Anions and Carboxylic Acids Determination

As a part of preliminary studies, the possibility of quantitative oxidation of aldehydes to carboxylic acids and their determination by isocratic ion chromatography with conductometric detection has been tested. A methodology for the simultaneous separation of inorganic anions (F-, Cl-, NO2-, Br-, NO3-, PO43-, SO42-) and carboxylic acids (formate, acetate and oxalate) by using isocratic ion chromatography with conductivity detection have been developed and validated. Six anion-exchange columns were tested. The best results were achieved for Dionex IonPac AS10 column with 50mM NaOH eluent and flow rate of 1.0 mL∙min-1.

Analysis of degradation products of nerve agents in biological fluids by ion chromatography-tandem mass spectrometry.

The detection of hydrolysis products of nerve agents (alkyl methylphosphonic acids; RMPAs) in biological samples from victims is important to confirm exposure to nerve agents. However, analysis of RMPAs is difficult due to their high hydrophilicity. The aim of this study was to develop ion chromatography-tandem mass spectrometry (IC-MS/MS) methods using commercially available equipment and columns to analyze RMPAs in human urine and serum with high sensitivity and without using complicate techniques. A Dionex IonPac AS11-HC anion-exchange column was used to analyze six RMPAs (MPA, EMPA, IMPA, iBuMPA, CHMPA, and PMPA). For pretreatments of biological fluids, we developed two pretreatment methods (Method 1: dilution and ultrafiltration; Method 2: removal of chloride ions with Ag cartridges). Six RMPAs including highly hydrophilic methylphosphonic acid and ethyl methylphosphonic acid could be analyzed with sufficient retention times and peak shape. The detection limits of RMPAs were improved using Dionex OnGuard II Ba/Ag/H cartridges and MetaSEP IC-Ag cartridges (urine: 0.5-5ng/mL; serum: 1-5ng/mL). These methods were also applied to the test samples for the Organisation for the Prohibition of Chemical Weapons Biomedical Proficiency Tests. RMPAs could be sufficiently analyzed by IC-MS/MS. In addition, the limits of detection were superior to those obtained in our previous study involving LC-MS/MS or derivatization-LC-MS/MS method. For analysis of biological samples, an appropriate pretreatment method can be chosen according to the amount of sample available for analysis and expected RMPA concentrations.

Determination of five water-soluble anions in solid and liquid aerosols by ion chromatography with a filter membrane and condensation collection

水溶性离子是固、液气溶胶的重要组成部分,对于气溶胶的理化性质和空气质量具有重大影响,研究水溶性离子的含量对于大气环境的污染与防治具有深远意义。该研究建立了一种滤膜冷凝收集-离子色谱技术采集固体气溶胶和液体气溶胶并测定其中的5种水溶性阴离子(Cl-、F-、NO3-、NO2-、SO42-)含量的方法。首先,采用固体颗粒过滤器和冷凝收集法分别收集固体气溶胶和液体气溶胶,固体气溶胶以固体颗粒物的形式被收集在固体颗粒过滤器内,液体气溶胶以冷凝液的形式在冷阱中被收集。其次,以离子色谱法对固、液体气溶胶中的水溶性阴离子含量进行检测。在以Dionex IonPac AS11-HC-4 μm作为分析柱,流速为1 mL/min,柱温为30 ℃,淋洗液氢氧化钾(KOH)浓度在0~40 min内由1 mol/L线性增至25 mol/L,进样量100 μL的条件下,各离子在40 min内有效分离,5种阴离子在0.1~10 mg/L范围内线性关系良好(相关系数为0.9992~0.9997),检出限低(0.02~0.04 mg/L)。对样品采集条件(采样时间、采样温度和采样流量)进行了优化,结果表明,在采样时间2 h、采样温度-13 ℃、采样流量1.0 L/min的条件下,可获得较为满意的结果。在优化的条件下分别对实际样品的两类溶胶中的5种阴离子含量进行了检测,测得实际样品的液体气溶胶中5种阴离子含量分别为5.7402 μg/m3 (F-)、1.1599 μg/m3 (Cl-)、3.3233 μg/m3 (NO2-)、2.4861 μg/m3 (NO3-)和0.9745 μg/m3 (SO42-),固体气溶胶中5种阴离子含量分别为14.1037 μg/m3 (F-)、5.0398 μg/m3 (Cl-)、9.3052 μg/m3 (NO2-)、8.4528 μg/m3 (NO3-)和5.6314 μg/m3 (SO42-)。该方法可应用于实际的大气检测中,也为其他离子的采集和分析条件的摸索提供了方法。

New Method for Simultaneous Arsenic and Selenium Speciation Analysis in Seafood and Onion Samples.

This paper presents a new method for the simultaneous speciation analysis of arsenic (As(III)-arsenite, As(V)-arsenate, DMA-dimethylarsinic acid, MMA-methylarsonic acid, and AsB-arsenobetaine) and selenium (Se(IV)-selenite, Se(VI)-selenate, Se-Methionine, and Se-Cystine), which was applied to a variety of seafood and onion samples. The determination of the forms of arsenic and selenium was undertaken using the High-Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC–ICP–MS) analytical technique. The separation of both organic and inorganic forms of arsenic and selenium was performed using two analytical columns: an anion exchange column, Dionex IonPac AS22, containing an alkanol quaternary ammonium ion, and a double bed cation–anion exchange guard column, Dionex Ion Pac CG5A, containing, as a first layer, fully sulfonated latex for cation exchange and a fully aminated layer for anion exchange as the second layer. The ammonium nitrate, at pH = 9.0, was used as a mobile phase. The method presented here allowed us to separate the As and Se species within 10 min with a suitable resolution. The applicability was presented with different sample matrix types: seafood and onion.

Ultra-trace determination of oxyhalides in ozonated aquacultural marine waters by direct injection ion chromatography coupled with triple-quadrupole mass spectrometry

A direct, robust, accurate and highly sensitive method for oxyhalide species in natural waters, including seawater, using suppressed ion chromatography coupled with mass spectrometry (IC-MS) is described. The method utilised a high capacity, high efficiency anion-exchange column (Dionex IonPac AS11-HC, 4 mm, 2 × 250 mm), with the separation achieved using an electrolytically generated potassium hydroxide gradient, delivered at 0.380 mL min−1. Applying the method, detection limits for iodate, bromate, and chlorate in seawater after direct sample injection (20 μL injection volume, samples diluted 10-fold), were 11, 30 and 13 ng L−1 (ppt), respectively. Standard addition calibrations to ozonated seawater samples were linear, in all cases R2 > 0.999 (n = 10), with intra-day repeatability of 3.7, 11.2 and 1.8 % RSD (n = 10) for a low-level standard mixture (0.30 μg L−1 of iodate, 0.15 μg L−1 of bromate, and 1.50 μg L−1 of chlorate). The method was applied to the analysis of seawater samples taken pre- and post-disinfection points within a recirculating aquacultural system. Iodate, bromate and chlorate were detected as the main oxyanionic disinfection by-products, demonstrating the practical utility of the new method as a valuable tool for monitoring changes to seawater composition following disinfection treatments.

Yaygın İnorganik Anyonların Çay Örneklerinde İyon Kromatografik Tayini

A simple, rapid, and accurate technique based on ion chromatography coupled with a suppressed conductivity detector was described to determine the inorganic anions – fluoride, chloride, nitrite, bromide, nitrate, phosphate, and sulphate in tea bag samples. A Dionex IonPac AS9-HC 4×250 mm analytical column with a Dionex IonPac AG9-HC 4×50 mm guard column was used for the chromatographic separation of the analytes. The eluent was 12 mM KOH and the limit of quantification for the inorganic anions were in the range 0.02 - 0.20 mg L-1. Tea infusions were prepared traditionally by pouring the tea bags with 200 mL of boiling ultra-pure water. Influence of the brewing time on the extraction of inorganic anions from the tea bags was examined and optimum brewing time was determined as 6 min according to the results obtained. Finally, the ion chromatographic technique was applied to analyze the inorganic anions in some black, green, and white tea bag samples and five anions were determined quantitatively. The nitrite and bromide amount in all the samples were well below the quantification limit of the method.

Simultaneous Species Analysis of Arsenic, Selenium, Bromine, and Iodine in Bottled Drinking Water and Fruit Juice by High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry.

A method for the simultaneous determination of arsenobetaine, arsenite, arsenate, dimethylarsinic acid, monomethylarsonic acid, selenite, selenate, bromate, bromide, iodate, and iodide in bottled drinking water and fruit juice samples was established by using high-performance liquid chromatography-inductively coupled plasma mass spectrometry. The separation of eleven compounds was performed on an ion exchange chromatography column (Dionex IonPac AS14) with 20 mmol L-1 (NH4)2CO3 (pH 10) and 50 mmol L-1 (NH4)2CO3 (pH 10) as a mobile phase. The limits of quantification of the method were 0.17 - 1.2 μg L-1 for the test compounds in bottled drinking water and 0.34 - 2.4 μg L-1 in fruit juice. The average recoveries ranged from 85.8 to 102.2%, and the relative standard deviations (RSDs) obtained in fortification recovery studies were generally <4.2% for bottled drinking water samples. The average recoveries ranged from 88.1 to 118.0% (except for iodate) for fruit juice sample.

Determining Potassium Bromate in the Inhalable Aerosol Fraction in Workplace Air with Ion Chromatography.

BackgroundThe article presents the results of studies performed in order to develop a new method of airborne potassium bromate(V) determination at workplaces.MethodsThe method is based on a collection of the inhalable fraction of potassium bromate(V) using the IOM Sampler, then extraction of bromates with deionized water and chromatographic analysis of the obtained solution. The analysis was performed using ion chromatography with conductometric detection. The tests were performed on a Dionex IonPac®AS22 analytic column (250 × 4 mm, 6 μm) with AG22 precolumn (50 × 4 mm 11 μm).ResultsThe method provides for potassium bromate(V) determination within the concentration range of 0.043 ÷ 0.88 mg/m3 for an air sample of 0.72 m3 in volume, i.e., 0.1–2 times the exposure limit value as proposed in Poland. The method was validated in accordance with PN-EN 482. The obtained validation data are as follows: measuring range: 3.1–63.4 μg/mL, limit of detection (LOD) = 0.018 μg/mL and limit of quantification (LOQ) = 0.053 μg/mL. The developed method has been tested in the work environment, on laboratory employees having contact with potassium bromate(V).ConclusionThe analytical method allowed the determination of the inhalable fraction of airborne potassium bromate(V) at workplaces and can be used to assess occupational exposure.

Uncertainty budget for the ion chromatographic determination of fluoride and chloride in nuclear grade boron carbide and elemental boron employing ultrasound-assisted dispersive solid–liquid extraction

A simple, rapid, and accurate method for the determination of trace level anionic impurities of fluoride and chloride in nuclear grade boron carbide and elemental boron is presented. The method involves ultrasound assisted dispersive solid–liquid extraction (UA-SLE) of fluoride and chloride from sample matrix, followed by determination of the analytes using ion exchange chromatography in suppressed conductivity mode. A Dionex IonPac AS11-HC anion exchange analytical column connected in series with an IonPac AG11-HC guard column has been employed for fluoride and chloride separation with 15 mM NaOH as the eluent. The limit of detection for fluoride and chloride is 1.0 and 2.0 µg g−1, respectively, and the relative standard deviation is 4% for the method. The present analytical method has a higher throughput. The recovery of added anions was in the range of 93–105%. The method was validated using ASTM method of pyrohydrolysis and ion selective electrode analysis. The method developed, eliminated the use of chemical reagents required in the case of regular sample pretreatment procedures. An uncertainty budget is presented for analysis using bottom up approach. Major contributor to the combined uncertainty was found to be the calibration intercept and instrumental response in the case of fluoride and chloride, respectively.

Quantifying indium with ion chromatography in hydro- and biohydrometallurgical leaching solutions.

A methodology has been developed to chromatographically quantify indium in polymetallic (bio)hydrometallurgical processing solutions using the Dionex IonPac CS5A column and pyridine-2,6-dicarboxylic acid eluent. Cu(II) and In(III) could be separated by elevating the column temperature to 45°C. The comparatively low stability constant of the In-eluent complex (log K2 =3.8) required typical leaching samples to be diluted in the eluent rather than acid or water to overcome ligand competition between components of the sample solution and the eluent. The methodology was applied to leachates from (bio)hydrometallurgical processing of oxidic flue dust residues and sulfidic zinc ores, where both are promising candidates for the recovery of indium from low grade ores and metallurgical wastes. Indium, ferrous iron, ferric iron, copper, zinc, nickel, and manganese concentrations could be simultaneously quantified. The method was found suitable for samples containing at least 0.25mg/L indium and an iron to indium ratio of up to 100:1.

Generic Ion Chromatography–Conductivity Detection Method for Analysis of Palladium Scavengers in New Drug Substances

The revolution of palladium-catalyzed reactions in the synthesis of new molecules has created an unprecedented need for highly efficient palladium (Pd) removal processes. Metal scavengers with very selective and efficient removal properties are being extensively applied across process research and development (PRD however, developing methodologies to detect a variety of Pd scavengers in multicomponent reaction mixtures is currently considered an emerging challenge in pharmaceutical analysis. Herein a simple and efficient generic ion chromatography–conductivity detection (IC–CD) method on a Dionex IonPac AS19 column in conjunction with a fully aqueous eluent profile (potassium-hydroxide-based) capable of chromatographically resolving over 10 Pd scavenger species commonly used in PR&D workflows is described. Computer-assisted separation modeling using an ACD Labs/LC si...

Indirect pulsed electrochemical detection following high-performance reversed-phase liquid chromatography

For detection using pulsed electrochemical detection (PED), analytes must preadsorb to the working gold electrode. Indirect pulsed electrochemical detection (InPED) exploits this requirement by including a PED-active reagent in the mobile phase. The background signal provided by oxidation of this reagent is attenuated by the adsorption of analyte molecules to the electrode. In this paper, a method has been developed to allow the use of InPED in combination with high-performance reversed-phase liquid chromatography (RPLC). Biotin was used as a probe molecule to determine that the use of acetonitrile as the organic modifier in the mobile phase provided superior results to the use of methanol. In addition, a silica-based, high surface area C18 column (i.e., Varian Pursuit XRs) was found to give better results than a polymer-based reversed-phase column (i.e., Dionex IonPac NS1). Optimized experimental conditions were used todetermine lipoic acid, tiopronin, and penicillamine, obtaining detection limits of ≤1 μM (30 pmol injected). The analytical utility of RPLC-InPED was demonstrated by an assay of an over-the-counter-formulation containing lipoic acid.

Ion chromatography with conductometric detection for quantitation of formic acid in Polish bee honey

Honey is a bee product that plays an important role in human diet. Its beneficial health properties are widely known. Honey is a substance of complex composition. One of many groups of naturally occurring compounds in honey are organic acids, among them formic, oxalic and lactic acids, exhibiting impact to varroosis – parasitic bee disease when occur at the excess. Therefore, such acids are used by beekeepers as natural medicines against varroa mites. Improper apiarian practice can increase the content of acids in honey and deteriorate its quality. The problem of the occurrence of elevated levels of formic acid in bee honey is discussed. Formic acid exhibits particularly high efficacy to kill the varroa mites and is often used in apicultural practice. The applicability of ion chromatography with conductivity detection to the determination of formic acid in bee honey was investigated. Rapid and selective method, using anionic Dionex IonPac AS9−HC column and 9.0 mmol/L Na2CO3 as the eluent, is presented. Formic acid concentrations in the analyzed samples of six commercially available Polish bee honeys of different origin range from 20.09 ± 2.62 mg/kg in acacia honey up to 248.58 ± 2.62 mg/kg in buckwheat honey.

Simultaneous Determination of Ammonium Ion, MDEA, Common Metal Cations and Dialkyl Amines in Amine Processing Solution by Ion Chromatography

In the present study a new method has been developed for the separation and estimation of common metal cations (Li+, Na+, K+), Methyl diethanolamine (MDEA) (and types of alkyl amines) and their degradation products including ammonium ion used in amine processing solution. The acid treating amines such as dimethyl amine (DMA), Diethyl amine (DEA), Dipropylamine (DPA), Diisopropyl amine (DPA) and Dibutyl amine (DBA) in amine processing solution have been separated by Ion Chromatography (IC) using conductivity detector in single column mode. Desired separation was achieved within 20 min using mixture of 3 mM CH3SO3H+CH3CN (90:10) as mobile phase on Dionex Ion Pac SCS1 column at temperature of 30°C. Calibration curve for all the cations (Li+, Na+, K+, NH4+) in the concentration range of 2.5-20 ppm and 3-100 ppm for dialkyl amine were plotted and it was observed that the correlation coefficient (R2) list in between 0.9980 and 1.0. Relative Standard Deviation (RSD) observed for all the abovementioned cations were in the range of 0.81-2.33%. Recovery of all the cations at different concentrations of amine processing matrix was in the range of 80-120%. The lower Limit of Detection (LOD) for Li+, Na+, K+, NH4+ was 0.5 ppm, and for MDEA and all above alkyl amines was 1.0 ppm. The operational simplicity of this method in terms of accuracy and precision can be a good alternative for the estimation of above ions in amine processing solution.