The neutral lipids of the skin of the Rhino mutant mouse consist mainly of fatty acid esters of sterols, fatty alcohols and 1,2-alkane diols, with strikingly low amounts of triacylglycerols. Fatty acids of wax and sterol esters were predominantly even chain monounsaturates (63%) between C 16 and C 36 with a surprisingly high proportion of long chain lengths: the principal peaks corresponded to C 32, C 34, C 18, C 30, and C 22 monoenes. The fatty alcohols showed a somewhat similar pattern, but with an even greater preponderance of long chain lengths and only small proportions shorter than C 24. Sterols included cholesterol, as expected, but only to the extent of about 28%; the larger fraction was shown to be lathosterol (5α-cholest-7-en-3β-ol). The largest single fraction (35.6%) of cutaneous lipids consisted of fatty acid esters of 1,2-alkane diols. The 1,2-alkane diols were completely saturated and included odd and even chain lengths, both straight and branched, in the C 16–C 24 range: predominant peaks were C 20, C 22(iso), C 16, and C 22. Fatty acids of diol esters ranged between C 14–C 36 with major concentrations of C 18, C 22, C 32, and C 34 monounsaturates and C 20 and C 16 saturates.