Dinoflagellate Cyst Assemblages Research Articles

Distribution of dinoflagellate cyst assemblages in surface sediments of Magellan fjords and channels (Patagonia, Chile) with a focus on harmful species: An overview on environmental scenario

Several dinoflagellate species form benthic resting cysts in their life cycle. In order to study the composition, abundance and distribution of cyst assemblages, with a focus on harmful species, thirty-one surface sediment samples from the Magellan fjords and channels system were analysed. In situ (temperature, salinity, inorganic nutrients, dissolved oxygen, organic matter, and chlorophyll-a) and five-year (salinity, temperature, dissolved oxygen, and chlorophyll-a) measurement data were analysed to obtain an overview on environmental scenario. A total of 56 cyst morphotypes were recorded, some of which were identified for the first time for the Chilean coast (Dactylodinium cf. arachnoides, Impagidinium cf. velorum, Archaeperidinium sp., Protoperidinium haizhouense, Protoperidinium sinuosum, Protoperidinium tricingulatum, and Dubridinium cf. ulsterum). In vitro germination of cysts allowed the vegetative form of the species of the order Suessiales to be determined. Total cyst concentrations were highly variable, ranging from 145 to 5453 cysts mL−1 of wet sediment (mean 1246 ± 1061 cysts mL−1), with the lowest cyst values in the area adjacent to the Southern Patagonian Ice Field. Pentapharsodinium dalei and Protoperidinium conicoides generally dominated (%) the cyst assemblages. The harmful species Alexandrium catenella and Alexandrium ostenfeldii were not abundant and sparsely distributed, while Protoceratium reticulatum was widespread and highly abundant with Gonyaulax spinifera in Inutil Bay (Magellan Strait). Cluster and nMDS analyses performed with cyst concentrations of the 56 taxa in the 31 stations formed seven cyst assemblage clusters, reflecting high environmental variability. Canonical correspondence analyses performed with cyst concentrations and in situ and long-term environmental data showed different ecological signals, demonstrating that in situ parameters must be used carefully when studying cysts in surface sediments.

Bajocian–Bathonian dinoflagellate cyst assemblages from the Middle Atlas, Morocco: Palynostratigraphic and paleoenvironmental implications

The Bajocian–Bathonian transition has been identified for the first time in the Skoura syncline of the folded Middle Atlas of Morocco based on dinoflagellate cyst and palynofacies analysis of the Ich Timellaline/Bou Akrabene Formation carbonates. This palynological study involved 109 samples of marls and limestones whose organic content yielded diverse and well-preserved dinoflagellate cyst assemblages comprising 68 taxa including stratigraphic marker taxa. Two association biozones have been defined for the late Bajocian–early Bathonian interval. The Cribroperidinium crispum-Ctenidodinium cornigerum (CC/CC) biozone is defined between the base of the Recifa Formation (Upper Bajocian) and the Ich-Timellaline / Bou Akrabene Formation (Upper Bajocian–Lower Bathonian). The second association biozone of Ctenidodinium combazii and Dichadogonyaulax sellwoodii (CC/DS) corresponds to the upper interval of the FD section (top of the Ich-Timellaline / Bou Akrabene Formation and the base of the El Mers I Formation). These two biozones were correlated with the late Bajocian–early Bathonian biozones defined in the Sub-Boreal (northwest Europe), Tethyan, and Australian domains. Close similarity between the Moroccan Middle Atlas, the Tethyan, and the Sub-Boreal domain associations has been noted. Quantitative analysis of organic matter constituents has allowed the paleoenvironmental reconstruction of the late Bajocian–early Bathonian. The organic residues of the studied samples recorded an increased land-derived phytoclasts dominance compared to amorphous organic matter and palynomorphs, indicating a proximal oxic shelf depositional environment with high terrestrial and freshwater influx during the late Bajocian–early Bathonian. During the Late Bajocian, the depositional environment corresponds to a proximal continental shelf with fluctuations from a distal to a marginal/stagnant environment.Below the Upper Bajocian–Lower Bathonian boundary, a significant marine incursion, or rather a transgression, is recorded in the studied sediments attested by an important marine fraction and dinoflagellate cysts abundance, which probably corresponds to the last Bajocian Maximum flooding surface (MFS).During the early Bathonian, the depositional environment evolved towards a distal continental shelf with an increasing marine fraction including dinoflagellate cysts and high species diversity. This may be related to the rising sea level which corresponds to the first Bathonian eustatic elevation. The proximal /marginal conditions are restored at the uppermost part of the section.

Late Quaternary Ponto-Caspian dinoflagellate cyst assemblages from the Gulf of Corinth, Central Greece (eastern Mediterranean Sea)

We present here the first long Quaternary record of organic-walled dinoflagellate cyst assemblages analysed from sediment cores retrieved during the International Ocean Discovery Program (IODP) Expedition 381 in the Gulf of Corinth. Site M0078A is located in the central part of the Gulf of Corinth (GoC), a semi-isolated marine basin that was repeatedly isolated and reconnected to the Mediterranean Sea during the Quaternary glacial/interglacial cycles. Our results show that dinoflagellate cysts are sorted in two major ecogroups, each group alternating between marine and isolated/brackish conditions. The marine intervals are characterised by high dinocyst diversity whereas the isolated intervals are dominated by taxa thriving in low-salinity conditions such as Spiniferites cruciformis and Pyxidinopsis psilata. In several of these assemblages, S. cruciformis is so prevalent that it forms almost monospecific assemblages. The low salinity dinocyst assemblages are reported for the first time outside the Ponto-Caspian region and they show a close affinity to modern assemblages from the Black Sea, Caspian Sea and Marmara Sea. The alternations between marine and brackish conditions recorded in the Gulf of Corinth reflect changes in surface water salinity (SSS) and temperature (SST), in response to the Quaternary glacial-interglacial cycles. These seem to be in good agreement with regional and global marine isotope and sea-level records. Our findings suggest that the study region sensitively responds to climate forcing at orbital time scales and that local factors most likely drive shifts in dinoflagellate species composition and diversity.

Strong regionalism in dinoflagellate cysts in recent sediments from the Chukchi-East Siberian Seas, Arctic Ocean

The Pacific sector of the Arctic Ocean, a region with the fastest sea-ice loss in recent decades, is experiencing unprecedented biological regime shifts in its marine ecosystems today. However, a shelf-wide characterization of marine plankton communities over this region and environmental processes behind it remain largely unresolved, despite phytoplankton phenology is now considered to be an important factor for assessing how Arctic Ocean interacts with global climate change. Here, we present the first detailed description of recent dinoflagellate cyst assemblages in 32 surface sediment samples from the Chukchi–East Siberian Sea shelves, the Pacific Arctic Ocean, to examine their biogeographic patterns and associated environmental forcing factors. We find a strong regionalization in the species composition and distribution along the shallow-shelf cyst accumulation zone; in the Chukchi Sea sector, northward flow of warm-Pacific water along with extreme seasonality, sufficient nutrient supplies and higher light availability leads to a large occupation of cosmopolitan, opportunistic-nature taxa, whilst the East Siberian Sea sector with more extensive sea-ice cover and lower temperatures is primarily dominated by “round brown spiny cyst” taxa that have a strong affinity for cold, polar conditions. Specifically, an exclusive dominance of heterotrophic taxa at the East Siberian Sea region is inferred to be closely tied to Atlantic-origin water intrusions that lift nutrient-rich, cold bottom water up to oligotrophic surface layer to grow diatoms and other prey organisms. The observed contrast in this geographical “hot spot”, where the Pacific- and Atlantic-origin waters face each other, clearly manifests a growing expansion of southern waters into an increasingly ice-free Arctic Ocean that reshapes Arctic Ocean biogeography from the base of marine food chains. Our study highlights considerable potential of dinoflagellate cysts as a valuable environmental proxy to indicate complex interactions between ocean physics and marine biology under the rapidly changing Arctic climate system.

Correlation of Middle to Upper Eocene Units from High Latitude Southwestern Atlantic Sites

The Palaeogene was a time of important climatic and palaeoceanographic changes. To understand these modifications in the Southern Hemisphere, and especially those related to the opening of the Drake Passage (DP), is essential to correlate the lithostratigraphic units of Eocene age in areas adjacent to this passage, such as those from the Austral-Magallanes Basin (to the north of the DP) and James Ross Basin (to the south of the DP). For many years, there was no correlation between these units due to the absence of reliable chronostratigraphic and biostratigraphic information. Recently, other authors published new detrital zircon U/Pb data for the Austral-Magallanes Basin and introduced important modifications to the previous stratigraphic scheme. Subsequently, due to the reinterpretation of the new available isotope data together with additional U/Pb ages from Chilean sections, the recent chronostratigraphic model was modified again, including not only the Austral-Magallanes Basin units but also those from the James Ross Basin. In the light of an updated quantitative dinoflagellate cyst dataset from southern South America and northern Antarctic Peninsula we performed a compositional Principal Component Analysis to evaluate and discuss the different age models proposed for the studied sections. We also compare our results with other assemblages from southern Chilean sections, Malvinas (Falkland) Plateau, and the South Orkney sites. We conclude that this analysis comparing different Eocene units from areas adjacent to the DP shows a high correlation between the dinoflagellate cyst assemblages considered to be Bartonian in age in the last proposed stratigraphic scheme.

The abundance and species assemblages of dinoflagellate cysts at sediments in the three estuaries of the Southern part of South Sulawesi

Harmful algal blooms (HAB) are a phenomenon of drastically increasing phytoplankton populations, which can cause problems in aquatic ecosystems. Dinoflagellate cysts are a type of phytoplankton that are the main source of causing the HAB phenomenon; this occurs due to unfavorable environmental conditions. The Southern Estuary of South Sulawesi has high fishery resources and high primary productivity. The coastal areas around these waters are widely used for anthropogenic activities that trigger nutrient increases. There are no studies on HAB, especially the abundance and community structure of dinoflagellate cysts at this location. This study aims to identify the types, abundance, and species assemblages of dinoflagellate cysts in the three estuaries of southern South Sulawesi. Sampling was carried out at three locations, namely the Pappa River Estuary, the Kelara River Estuary, and the Calendu River Estuary, in January 2022. Each location consists of 4 stations, 3 sub-stations with 3 repetitions sampling for each substation. Results showed that there were 34 species of dinoflagellate cysts belonging to 6 families, namely Gonyaulacaceae, Gymnodiniaceae, Osteropsidaceae, Peridiniaceae, Polykrikaceae, and Protoperidiaceae. The highest abundance of dinoflagellate cysts was at the Calendu River estuary station III, accounting for 82 cysts g−1, while the lowest abundance of dinoflagellate cysts was found at Pappa River estuaries stations I and II, accounting for 8 cysts g−1. The level of diversity, uniformity, and richness of dinoflagellate cysts was not significantly different in the three study sites. The family Osteropsidaceae was the most common type of dinoflagellate cyst found in the three study sites. The Osteropsidaceae family is a type of dinoflagellate cyst that contains toxins, so it can be assumed that the three research sites have the potential for HAB.

Diatom and dinoflagellate cyst distribution in surface sediments of the Chukchi Sea in relation to the upper water masses

Diatom and dinoflagellate cyst analyses were performed on 22 surface sediment samples from the Chukchi Sea to document their geographical distributions in one of the most understudied sections of the Arctic Ocean and to examine the influence of upper water masses on these two major groups of phytoplankton. Total concentrations vary from 0.9 to 5.9 × 106 valves g−1 for diatoms and from 0.8 to 12.5 × 103 cysts g−1 for organic-walled dinoflagellate cysts, with the highest values for both groups observed in the southern part of the Chukchi Sea and away from the Bering Strait. Well-preserved microfossils were recovered, with a total of 35 and 88 taxa of dinoflagellate cysts and diatoms, respectively. The most abundant diatoms are Paralia sulcata, Thalassiosira antarctica, Thalassiosira nordenskioeldii, and resting spores of Chaetoceros spp., whereas cysts of phototrophic Alexandrium spp., Operculodinium centrocarpum sensu Wall and Dale (1966), and heterotrophic Islandinium minutum and Brigantedinium spp. were most common in the dinoflagellate cyst assemblages. Cysts of HAB-causing Alexandrium spp. were found in most of the samples, with the highest abundances in Herald Canyon where they contribute ∼56.6% to the cyst assemblage. As expected, cysts produced by heterotrophic dinoflagellates were more abundant where sedimentary diatom concentrations were the highest.Statistical analysis identified three major diatom and dinoflagellate cyst clusters: 1. Sites influenced by the Alaska Coastal Current in the eastern part of the Chukchi Sea are characterized by high abundances of P. sulcata and O. centrocarpum sensu Wall and Dale (1966); 2. The western part and Herald Canyon in the northern part of the Chukchi Sea are distinguished by diatoms Chaetoceros spp., T. antarctica and dinoflagellate cysts of Alexandrium spp. and affected by the Siberian Coastal Current and Bering Shelf Water; and 3. Assemblages in the southern part of the Chukchi Sea are recognized by noticeable abundances of T. nordenskioeldii and cryophilic diatom taxa, and dinoflagellate cysts I. minutum, as well as by overall lower percentages of cysts of Pentapharsodinium dalei and Brigantedinium spp. This work revealed the potential applicability of the combined use of diatoms and dinoflagellate cysts for reconstructions of past dynamic water mass changes in the Chukchi Sea.

Dinoflagellate cysts from the Upper Cretaceous (Campanian–Maastrichtian) from The Naze Peninsula, James Ross Island, Antarctica

We present the results of the quantitative and qualitative study of dinoflagellate cysts in outcrop samples from a section of the Snow Hill Island Formation (SHF) in James Ross Island, Antarctic. Dinoflagellate cysts assemblages are abundant and dominated by gonyaulacoid taxa. The last occurrence of the dinoflagellate cyst Kallosphaeridium? helbyi and Chatangiella granulifera, together with the first occurrence of Pterodinium cretaceum, indicates a probable late Campanian age (∼76.4–∼72.1 Ma) for the lower strata. Whereas the first occurrence of Manumiella bertodano and the last occurrence of Odontochitina operculata, Xenascus ceratioides, and Stiphrosphaeridium anthophorum indicate an early Maastrichtian (∼72.1–∼70.0 Ma) age for the upper strata. Our results, combined with paleobotanical and palynological published data, indicate medium–high continental and marine productivity with temperate paleoclimate free of glaciers for this interval. Lithological and paleontological data indicate mainly inner neritic marine depositional environments. The predominance of shales in the lower part of the lower Maastrichtian interval indicates a slightly deeper environment. In contrast, in the middle part of the lower Maastrichtian, a shallower, transitional environment is marked by the presence of theropod dinosaurs and decapod crustaceans.

Sedimentological and palynological records since 10 ka BP along a proximal-distal gradient on the Armorican shelf (NW France)

New findings acquired in Armorican shelf (core MD08-3204 CQ: Bay of Quiberon and core VK03-58bis: South Glénan islands) depict Holocene paleoenvironmental changes since 10 ka BP through a multi-proxy dataset including sedimentological and palynological data. First, grain-size analyses and AMS-14C dates show a common sedimentary history for both study cores. The slowdown of the relative sea level (RSL) rise was accompanied by a drop of the sedimentation rates between ∼8.3 and 5.7 ka BP; the rates had been relatively higher at the onset of the Holocene. This interval led to the establishment of a shell-condensed level, identified in core VK03-58bis by the “Turritella layer” and interpreted as a marker for the Maximum Flooding Surface. Palynological data (pollen and dinoflagellate cyst assemblages) acquired in core MD08-3204 CQ argue for an amplification of the fluvial influence since 5.7 ka BP; the establishment of the Highstand System Tract (i.e., mixed marine and fluviatile influences on the platform) then accompanied the slowdown of the RSL rise rates. On the shelf, the amplification of Anthropogenic Pollen Indicators (API) has been better detected since 4.2 ka BP, due not only to the increase of human impact but also due to a stronger fluvial influence on the shelf during the Late Holocene. Palynological data, recorded on the 8.5–8.3 ka BP interval along an inshore-offshore gradient, also demonstrate the complexity of the palynological signals such as i) the fluvial influence that promotes some pollen taxa (Alnus and Corylus) from proximal areas and ii) the macro-regionalization of palynomorph sources in distal cores. In addition, the comparison of palynological tracers, including API, over the last 7 kyrs BP, with South Brittany coastal and mid-shelf sites subject to northern vs. southern Loire catchment areas, shows a major hydro-climatic effect on the reconstructed palynological signals. Strengthened subpolar gyre (SPG) dynamics, combined with recurrent positive North Atlantic Oscillation (NAO) configurations, appear responsible for increased winter precipitation and fluvial discharge over northern Europe, as in Brittany. Conversely, weakened SPG intervals, associated with recurrent negative NAO-like modes, are characterized by intensified winter fluvial discharge over southern Europe. Interestingly, at an infra-orbital timescale, we record major peaks of API during periods of strengthened (/weakened) SPG dynamics in sites subject to detritic-organic matter inputs from Brittany (/Loire) watersheds.

Middle Miocene (Serravallian; upper Badenian–lower Sarmatian) dinoflagellate cysts from Bad Deutsch-Altenburg, Vienna Basin, Austria

Middle Miocene (Serravallian; upper Badenian–lower Sarmatian) strata recovered in 10 cored boreholes (83 samples) from Bad Deutsch-Altenburg, Vienna Basin, Austria, were analysed palynologically for the first time. The strata belong to the Rabensburg Formation of the Baden Group. The lateral distribution of the boreholes in reference to a Mesozoic ridge makes this area interesting for studying various aspects such as distribution of deposits, stratigraphy and palaeoenvironmental reconstruction. A diverse and well-preserved in situ dinoflagellate cyst association has been identified. Middle Miocene age-diagnostic species including Cannosphaeropsis passio, Cerebrocysta poulsenii, Habibacysta tectata, Labyrinthodinium truncatum, Operculodinium? borgerholtense and Unipontidinium aquaeductum are recorded. Their occurrences allow correlation with dinoflagellate cyst biozonations on a regional scale. Based on the identified dinoflagellate cysts and by correlation with calcareous nannoplankton and ostracods, from the same set of samples, a Serravallian age – corresponding to a late Badenian and early Sarmatian age (regional Paratethys stages) – is confirmed. Reworked (Cretaceous and Paleogene) dinoflagellate cyst assemblages, also well preserved, were recorded abundantly from boreholes HA 521 and HA 573 (south-west of the Mesozoic ridge). In accordance with ostracods, the Badenian–Sarmatian boundary can be traced in the upper part of borehole HA 66 based on an abrupt change in the dinoflagellate cyst assemblages. The upper Badenian strata were deposited in a coastal to inner shelf environment with terrigenous (siliceous sand and clay) and carbonate sediments. The recorded dinoflagellate cysts reflect marine, tropical to warm-temperate climatic conditions. The composition of the recorded dinoflagellate cyst assemblages is very close to that of the Middle Miocene assemblages of the Mediterranean, indicating water exchanges between the Central Paratethys and the Mediterranean during the late Badenian (early Serravallian).

The role of physico-chemical interactions in the seasonality of toxic dinoflagellate cyst assemblages: The case of the NW Patagonian fjords system

Harmful algal blooms (HABs) are recurrent in the NW Patagonia fjords system and their frequency has increased over the last few decades. Outbreaks of HAB species such as Alexandrium catenella, a causal agent of paralytic shellfish poisoning, and Protoceratium reticulatum, a yessotoxins producer, have raised considerable concern due to their adverse socioeconomic consequences. Monitoring programs have mainly focused on their planktonic stages, but since these species produce benthic resting cysts, the factors influencing cyst distributions are increasingly gaining recognition as potentially important to HAB recurrence in some regions. Still, a holistic understanding of the physico-chemical conditions influencing cyst distribution in this region is lacking, especially as it relates to seasonal changes in drivers of cyst distributions, as the characteristics that favor cyst preservation in the sediment may change through the seasons. In this study, we analyzed the physico–chemical properties of the sediment (temperature, pH, redox potential) and measured the bottom dissolved oxygen levels in a “hotspot” area of southern Chile, sampling during the spring and summer as well as the fall and winter, to determine the role these factors may play as modulators of dinoflagellate cyst distribution, and specifically for the cysts of A. catenella and P. reticulatum. A permutational analysis of variance (PERMANOVA) showed the significant effect of sediment redox conditions in explaining the differences in the cyst assemblages between spring-summer and fall-winter periods (seasonality). In a generalized linear model (GLM), sediment redox potential and pH were associated with the highest abundances of A. catenella resting cysts in the spring-summer, however it was sediment temperature that most explained the distribution of A. catenella in the fall-winter. For P. reticulatum, only spring-summer sediment redox potential and temperature explained the variation in cyst abundances. The implications of environmental (physico-chemical) seasonality for the resting cysts dynamics of both species are discussed.

Distribution of modern dinocysts and pollen in the western Mediterranean Sea (Algerian margin and Gulf of Lion)

The Mediterranean Sea is generally described as an oligotrophic area where primary productivity is limited to a few coastal environments with nutrient-enriched fluvial input. However, several studies have revealed that the hydrology of the western Mediterranean has major seasonal productive patterns linked either to significant riverine input or to seasonal upwelling cells. This study aims to: i) discuss organic microfossils (i.e. pollen and dinoflagellate cyst assemblages, as well as other non-pollen palynomorphs) from two different productive areas of the western Mediterranean Sea, and ii) examine the importance of the interconnections between marine and continental influences responsible for modern palynomorph distributions. Based on 25 samples from the Gulf of Lion (GoL) and Algerian Margin, this study key findings are: i) that GoL marine productivity is driven by the combination of discharge from the Rhône River and seasonal upwelling mechanisms, ii) that the strong productive pattern of the northern African coast is driven by water density front mixings and related upwelling. These two patterns are discussed in the light of major links that provide a better understanding of the signatures of marine and continental bio-indicators. Typical differences in vegetation across the north-south climate gradient in the western Mediterranean Basin are highlighted by the larger ratio of Euro-Siberian to Mediterranean pollen taxa in the northern sector. Synoptic maps also illustrate the complex interactions of environmental drivers determining the distributions of continental and marine palynomorphs in the western Mediterranean Sea.

Eocene palaeoenvironments and palaeoceanography of areas adjacent to the Drake Passage: insights from dinoflagellate cyst analysis

AbstractA proper understanding of the palaeoceanographic evolution of the Drake Passage during the Palaeogene is hampered by the lack of precise tools to date and correlate the sedimentary units of areas adjacent to the region. In this work, considering recently published radiometric U–Pb dates, we revised the age of a previous dinoflagellate zones for the middle to upper Eocene units of the Austral–Magallanes Basin. The quantitative analysis of middle to late Eocene dinoflagellate cyst assemblages from different localities close to the Drake Passage allowed us to reconstruct the palaeoenvironmental conditions and the possible surface ocean currents during this time in the area. Assemblages dated between 41.3 and 38.1 Ma represent relatively warm waters in inner shelf settings, while those ranged between 36 and 35 Ma reflect coastal areas with cool, nutrient‐rich surface waters. The proposed surface ocean circulation pattern, based on dinoflagellate cysts distribution between 41.3 and 38.1 Ma, agrees with the results of a palaeoclimatic numerical model simulation performed with a Drake Passage shallow opening of 100 m depth. At c. 36 Ma, several Antarctic gonyaulacacean taxa tolerant to relatively warmer waters were replaced by some Antarctic peridinacean species better adapted to colder conditions. This change could be linked to a progressive deepening of the Drake Passage that is estimated to have reached 1000 m depth promoting a cooling in the South Atlantic. Such passage depth would have enabled stronger flows from the Pacific to the Atlantic Ocean, which is reflected by the increase of cosmopolitan species.

Climate-driven dinoflagellate cyst stratigraphy of the Oligocene-lower Miocene turbidite succession of the Lower Congo Basin (offshore NW Angola)

Biostratigraphic studies of cuttings from wells located in the Lower Congo Basin, offshore northwestern Angola, document with unprecedented wealth of data the organic-walled dinoflagellate cyst (dinocyst) assemblages of the Malembo Formation. The identification of a series of dinocyst events (highest and lowest occurrences, influxes and acmes) recurring in the same stratigraphic position in different wells and cross-calibrated by calcareous nannofossil data, provides a tool for age control and correlation of the Oligocene and lower Miocene in the equatorial and South Atlantic region.Proceeding downwards in the stratigraphy of the examined succession, the lowest occurrence of Pyxidinopsis pastilliformis, detected within the CN3 nannofossil Zone, approximates the Langhian-Burdigalian boundary, whereas the lowest occurrences of Sumatradinuim druggii and Sumatradinium hispidum occur at the base of the CN2 nannofossil Zone in the Burdigalian. The lowest occurrences of Sumatradinium soucouyantiae and Barssidinium taxandrianum fall within the Zone of acme of Hystrichokolpoma rigaudiae at the Burdigalian-Aquitanian transition, and immediately above the acmes of Cribroperidinium tenuitabulatum and Systematophora-Cleistosphaeridium group that are supposed to reflect climate cooling pulse Mi-1a. The presence of Chiropteridium spp. and Membranophoridium aspinatum is limited to the upper part of the Chattian. An influx of Tuberculodinium vancampoae, possibly related to a warmer interlude during a period of overall climate cooling, occurs in proximity of the Chattian-Rupelian boundary. The nearly synchronous highest occurrences of Gerdiocysta conopeum, Eocladopyxis peniculata and Heterosphaeridium sp. provide a robust infra-Rupelian biohorizon. Further down in the Rupelian, the influxes of Pentadinium laticinctutm and Distatodinium paradoxum are interpreted as responses to cooling pulses. The lowest occurrence of G. conopeum, detected in the basal part of the NP23 nannofossil Zone, corresponds to the global first appearance of this excellent Rupelian marker. Genera Deflandrea and Wetzeliella are noticeably missing in the studied material just as they lack in some coeval dinocyst assemblages reported in the literature from low paleolatitude Atlantic sections.The recorded alternation of influxes and acmes of Gonyaulacoid dinocyst taxa indicative of cooler and warmer water conditions was probably climate-driven, i.e. paced by cooling pulses and relatively warmer interludes characterizing the climate evolution during the Oligocene and early Miocene. Indeed, the lack of acmes of Peridinioid cysts suggests that climate, rather than constantly elevated nutrient levels ensured by the Congo River discharge, was the discriminant factor triggering the observed blooms of Gonyaulacoid cyst-forming taxa.The delivery of large volumes of sands into deepwater settings via gravity-driven fluxes was favored by sea-level drops and is ultimately associated with pulses of climate cooling and Antarctic ice-sheet growth, finally reflected by the composition of the dinocyst assemblages. In addition to paleoecological factors, hydraulic fractionation resulting from sedimentary processes that transported palynomorphs down the slope as part of turbidite fluxes, contributed to shape the palynological assemblages observed in this study.

Last Interglacial (MIS 5e) sea surface hydrographic conditions in coastal southern California based on dinoflagellate cysts

The first high resolution record of dinoflagellate cysts ∼110–155 kyr over Termination II and the Last Interglacial in the Santa Barbara Basin, California from ODP Hole 893A details a complex paleoceanographic history. Variation in cyst relative abundances, concentrations, and diversity reflects climatic and ocean circulation changes, and the assemblages are used to make qualitative reconstructions of sea surface temperature (SST) and annual primary productivity. The dominance of heterotrophic Brigantedinium spp. throughout the section indicates an important influence of coastal upwelling on the basin. Based on the dinoflagellate cyst assemblages, five zones are identified, which approximately correspond to the marine isotope stage boundaries and their associated changes in SST and sea level. Cooler intervals, MIS 6 and MIS 5d, are characterized by cold-water indicator species Selenopemphix undulata whereas thermophilic taxon Spiniferites mirabilis characterizes a warm MIS 5e. In contrast to other studies in the Pacific, the data exhibits a one to two-thousand-year cooling event ∼129 kyr that correlates to the Termination II sea level still-stand of the two-step deglaciation. A significant increase in cyst concentrations in the latest MIS 5e implies enhanced primary production due to increased seasonal upwelling and the warm, nutrient rich waters entering the basin after sea level stabilizes near modern levels. The hydrological evolution and cyst signal of the Last Interglacial is similar to the development of the Holocene in the Santa Barbara Basin, but the sustained presence of Spiniferites mirabilis across MIS 5e indicates SSTs were higher than modern conditions. • First high resolution dinocyst study in temperate northeastern Pacific during MIS5e. • SST, upwelling, and sea-level changes drive dinocyst variation in the SBB. • Spiniferites mirabilis and Selenopemphix undulata are best indicators of SST change. • Dinocyst paleoecology consistent with previous geochemical and palynology signals. • Termination II and MIS5e dinocyst assemblages are comparable to Holocene SBB cysts.

Marine Environmental Change Induced by Anthropogenic Activities – From a Viewpoint of Aquatic Palynomorph Assemblages Preserved in Sediment Cores of Beppu Bay, West Japan

Stratigraphic cluster analysis using aquatic palynomorphs preserved in the core sediments revealed a rapid eutrophication due to anthropogenic activities from the mid 1960s in Beppu Bay, East Kyushu, Japan. These assemblages were divided into three major units: BP-I, BP-II and BP-III, and also only dinoflagellate cyst assemblages were divided into the following four units in Beppu Bay: BP-A, BP-B, BP-C, and BP-D. Unit boundaries based on aquatic palynomorphs and dinoflagellate cysts were different except in the upper part, BP-III and BP-D, both of which clearly indicated anthropogenic eutrophication in both sea water and bottom sediments. On the other hand, in dinoflagellate cyst assemblages, Unit BP-A was characterized by stable occurrence of Spiniferites bulloideus and Spiniferites hyperacanthus, Lingulodinium machaerophorum of Gonyaulacales, and reduction of heterotrophic Peridinioid Brigantedinium spp. In Unit BP-C there was a clear decrease of L. machaerophorum. Unit BP-B was characterized by decreases of S. bulloideus, S. hyperacanthus, and L. machaerophorum, and little increase of Spniferites bentori. Unit BP-C was characterized by an increase in S. bulloideus and heterotrophic Peridinioid Echinidinium spp. Unit BP-D was subdivided into Subunit BP-D1 where dinoflagellate cysts showed a marked increase in S. bulloideus accompanied by the appearance of L. machaerophorum and Tuberculodinium vancampoae, and Subunit PB-D2 where there was a decrease of total dinoflagellate cysts. From the dinoflagellate cyst assemblages, the marine environment of the period of BP-A Unit was suggested to be warm and stable. However, L. machaerophorum started to decrease in BP-B. The clear decrease of L. machaerophorum suggest that the marine environment became cooler than that of Unit BP-A. Significant increases of S. bulloideus, S. hypearcanthus, L. machaerophorum, T. vancampoae, Brigantedinium spp., and Polykrikos kofoidii were characteristic of Unit BP-D. The increase in total dinoflagellate cyst density and the increase of the ratio of heterotrophic dinoflagellate cysts in Subunit BP-D1 are manifestations of the Oslo fjord Signal and Heterotroph Signal, respectively. In addition, the decrease in microforaminiferal lining that continued from Unit BP-C to Unit BP-D might indicate deterioration of the bottom sediment environment.

Assemblages of dinoflagellate resistance cysts and copepod eggs in superficial sediments at the upper Gulf of California

The diversity, abundance, and geographic distribution of dinoflagellate cysts in recent sediments serve as bioindicators to specific regions' environmental conditions and productivity. We present dinoflagellate cysts assemblages from 53 superficial sediment samples from the upper Gulf of California (uGC) obtained in July 2016 and February 2017. Forty-seven species were identified, of which twenty-seven are considered heterotrophic (Het) and twenty autotrophic (Aut). Ten species of this last group are potentially toxin producers (PTxS). The total concentration (Tot) of cysts in all the samples was 3481 cysts/g in 2016 and 4223 cysts/g in 2017. Aut represented 59%, Het 41%, while the PTxS represented ∼36.5% of the Tot cysts. The higher abundance of the Aut is likely to manifest the local high sea surface temperature and salinity. The increase in the Tot of cysts in the 2017 set was related to a Gymnodinium catenatum harmful algal bloom. Copepod eggs abundance was 7137 eggs/g in 2016 and 6555 eggs/g in 2017. The Aut Gymnodinium spp., Spiniferites spp., Operculodinium spp, and the Het Protoperidinium americanum, Quinquecuspis concreta, Selenopemphix nephroides, Brigantedinium spp., and Echinidinium spp., were typical in both seasons but differed on their concentrations between each season. The cysts distribution between both sampling periods reflects local currents and the sediment dynamics of the uGC, and the high species diversity in this area reflects the elevated primary productivity in this area.

DISTRIBUTION OF DINOCYSTS IN THE SURFACE SEDIMENTS OF SIDI MOUSSA LAGOON : (ATLANTIC COAST-MOROCCO)

Abstract. The Sidi Moussa lagoon, (32°52′0″ N / 8° 51′05″ W) is located on the Moroccan Atlantic coast between the cities of El Jadida and Safi about 15 km South of the Jorf Lasfar industrial complex, The climate of the lagoon is classified as hot temperate. It is characterised by an oceanic influence. This study of dinoflagellate cyst mapping is the first of its kind from the Sidi Moussa lagoon. the objectives of the present survey were (i) to evaluate to the spatial variation of dinoflagellate cyst assemblages in the sediment along the Sidi Moussa lagoon (ii) to assess the densities of its cysts (iii) to compare the cyst assemblages in this study with that of other sites. The data were collected in a scientific campaign by zodiac carried out from in April 2018 by core following a sampling network of 21 stations distributed randomly in the study area. The surface layer of the sediment cores (3 cm) was sliced and kept at 4° C until analysis. The highest total cyst abundance was 194 cysts. g−1 dry sediment. The Pearson statistical test revealed a positive and significant correlation between cyst abundance and water content, organic matter and of fine sediment. Our study showed the presence of morphotypes of potentially toxic species. The cysts of these species present in the sediment of the Sidi Moussa lagoon could germinate, when environmental conditions become favorable, and in turn could inoculate the water column with the subsequent bloom formation. This study confirms the usefulness of cyst analysis in the assessment of harmful bloom risk in this area important for oyster’s culture.

Biostratigraphic ages and depositional environments of the upper Oligocene to lower Miocene Veldhoven Formation in the central Roer Valley Rift System (SE Netherlands-NE Belgium)

Abstract Discussions on the age and the depositional environments of the Veldhoven Formation and its members are persistent in Belgium and the Netherlands. Uncertainties on stratigraphy and the constructive process of sediment accumulation continue today as a result of lack of data on this succession within the Roer Valley Rift System. The present study provides new information on the bio- and lithostratigraphy and facies from two boreholes based on dinoflagellate cyst taxa. The results were correlated by gamma-ray logs towards other key boreholes in the region and show a good consistency for stratigraphy and depositional environments for the members of the Veldhoven Formation. After marginal to restricted marine conditions in the latest Rupelian (early Oligocene), the start of deposition of the Veldhoven Formation marked the transition towards a higher sea level, expressed by increased glauconite contents and gamma-ray values. The Voort Member in the lower part of the Veldhoven Formation has an early to late Chattian (Late Oligocene) age and comprises predominantly shallow marine (fluctuating restricted to open marine) conditions. The lithology in the lower part of this unit is often very clayey but is coarsening upward into sands. The superjacent Wintelre Member has a latest Chattian to early Aquitanian (early Miocene) age. This member is distinct by its clayey nature which is expressed by relatively high gamma-ray values. Earlier studies suggest a deeper marine facies for the Wintelre Member compared to the Someren and Voort members. However, the dinoflagellate cyst assemblages in this unit are mostly dominated by a single genus indicating a restricted marine setting, including salinities that deviate from normal marine conditions, most probably due to minor ventilation by narrow or lack of connection to the Atlantic Ocean. A glacio-eustatic sea-level fall around the Oligocene/Miocene boundary limited the sea coverage to the strongest subsiding areas, where deposition of the Wintelre Member is recorded, while non-deposition or erosion occurred in the surrounding highs, hence creating an isolated (sub)basin. The superjacent Someren Member was deposited during the late Aquitanian to middle Burdigalian and consists of shallow to open marine clayey fine sands. Increasing clay contents indicate a gradual development towards a higher sea level, which coincide with upward increasing gamma-ray values. The biostratigraphic results of this study suggest that no major hiatuses are present in the differentially subsiding blocks of the Roer Valley Rift System during the late Oligocene to early Miocene.

Revision of the endemic dinoflagellate cyst genus Pontiadinium Stover & Evitt, 1978 from Lake Pannon and the Paratethys realm (Late Miocene–Early Pliocene, Central Europe)

The biota of the brackish-water Lake Pannon in the Pannonian Basin is characterised by remarkable endemism due to the isolated evolution of the lake for 8 myr after the last Miocene marine connection ceased (∼11.6 Ma). A conspicuous feature of this endemism is the large, probably ecophenotypic variation in the morphology of brackish-water dinoflagellate cysts that challenges taxonomy and complicates biostratigraphical and ecological interpretations. We conclude that a widely debated Lake Pannon genus, Pontiadinium, includes several proximate dinoflagellate cyst species with prominent apical and antapical protuberances, and we show how the genus differs from the morphologically similar gonyaulacacean cyst genera Impagidinium, Leptodinium, Cribroperidinium and Komewuia. The generic description of Pontiadinium is emended together with the species descriptions of Pontiadinium inequicornutum, Pontiadinium obesum and Pontiadinium pecsvaradanesis. A new species is described as Pontiadinium szentaiae sp. nov. from Našice (northern Croatia) that is characterised by unique trabeculate sutural septa formed from a beaded tegillum supported by columellae or rod-like luxuriae. The dinoflagellate cyst assemblages of the long-lived brackish-water Lake Pannon clearly demonstrate that dinoflagellate cysts in low-salinity, isolated epicontinental seas display greater morphological plasticity than their normal-marine relatives. The development of an antapical horn appears to be a previously undocumented example of phenotypic morphological features that developed in response to subnormal salinities within at least two dinoflagellate cyst genera endemic to Lake Pannon and the Post-Paratethyan seas of the Ponto-Caspian realm. This ecophenotypic variation resulted in a higher level of morphological adaptation, leading to the evolutionary development of new dinoflagellate cyst species and genera.