AIM or PURPOSE: The increasingly production of medical waste(MW) is a public health problem. The impact on human health and environment are very important. The aim of this study was to evaluate the practices and knowledges of Tunisian dentists about this field of interest.MATERIALS and METHOD: We conducted a cross sectional study. A self-administered questionnaire of 29 questions was published in a specific group of dentists in the social media. Data were collected immediately on GoogleForms and treated with SPSS.RESULTS: We collected 187 responses, of whom 82.8% were concerned about the impact of their practice on the environment. Practitioners who had been in practice for more than 10 years were more concerned about this issue (p=0.022).In 87,71% of cases, they stored the MW separetly, 12.3% outsourced to a waste management company. For the remaining, 95.12% of them disposed of in public bins. Most participants (87.3%) did not have a special container for sharps waste. Amalgam waste was disposed of with other waste for 35% of participants, increasing the risk of air and water contamination. The majority of participants used digital radiography (83.5%).Most participants (92.5%) had not received any training in healthcare waste management.CONCLUSION(S): There was a gap in the knowledge of proper MW management among Tunisian dentists. This issue must be a priority for the various national stakeholders involved, because protecting the environment is the responsibility of all of us. We must preserve this planet for future generations.