Background The development of 3-dimensional (3D) imaging provides exciting opportunities in oral and maxillofacial radiology and endodontics, specifically in regenerative endodontic therapies.1 Objectives The aim of this study was to explore the feasibility of creating a virtual 3D model of a molar root canal system by using micro-CT images to produce scaffolds with similar shapes with an open source slicer software. Materials and methods An extracted molar tooth was scanned by using the Bruker Skyscan micro-CT (Kontich, Belgium), at 10 µm resolution, 16 bits depth, and 360 degrees rotation, using a step-and-shoot detector motion at 80 kV. The images were reconstructed by using NRecon software and initially stored as TIFF (tagged Image File Format) files and later converted to DICOM (Digital Imaging and Communications in Medicine). The converted DICOM files were imported into the 3D slicer software. There were difficulties with segmenting these images as the file size was in excess of 1 gigabyte (GB), and the computer was not compatible. The segmentation was done by using 3D slicer software2,3 by selecting the region of interest (ROI) and then using the threshold tool to segment the root canal. All 3D models were stored as STL (stereolithography) files. For these kinds of files, it is recommended that extremely fast computers with a minimum random access memory (RAM) of 32 GB be used for the images to even be displayed. Preliminary results Preliminary results indicate that root canal systems can be segmented accurately from micro-CT images by using segmentation software. Future directions Future research will be expanded to validate these finding by measuring and comparing images of sectioned teeth and 3D printed models of root canals.
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