Digestibility Properties Of Starch Research Articles

Differences in starch digestive properties of cooked rice between four older and four current varieties

The replacement of rice varieties has been occurred in China over the past decades, with improving palatability serving as one of the main driving factors. Nevertheless, limited research exists on how this replacement has influenced the starch digestion properties of cooked rice, which play a critical role in determining the glycemic index, a key indicator of foods linked to the risk of non-communicable diseases. This study was conducted to determine the differences in starch digestive properties of cooked rice among four older and four current varieties, while also identifying the primary factors contributing to these differences. The results showed that the current varieties exhibited a 54% shorter active digestion duration and a 109% faster glucose production rate during starch digestion compared to the older varieties. These differences arose unintentionally from breeders’ efforts to meet consumer preferences for rice with a softer texture and were associated with decreased amylose content, increased amylopectin content, a lower amylose-to-amylopectin ratio, and increased breakdown viscosity in the current varieties than the older ones. This study highlights the necessity for increased awareness of the potential health risks linked to the accelerated starch digestion rate resulting from the development of softer-textured varieties in China.

Mechanism of protein component addition on digestive properties of starch in fermented barley

Previous studies have found that removing protein from fermented barley flour promotes starch hydrolysis. In this paper, we investigate how the addition of fermented barley proteins to fermented starch affects starch digestive properties and explore the underlying mechanisms. The results show that the starch-protein complex (with a mass ratio of 1:0.35) forms larger protein aggregates during the co-gelatinization of starch and protein, which limits the swelling of starch and thus inhibits starch digestion. Analysis of the peak positions in the infrared spectrograms revealed that the absorption peaks of the starch-protein complexes shifted from 3313.65 cm−1 to 3302.49 cm−1. The addition of sodium dodecyl sulfate (SDS) to some extent lowered the storage modulus (G′) and loss modulus (G″) of the mixed system. The proteins confer a stronger resistance to enzymatic hydrolysis on the starch through the formation of hydrogen bonds and participation in hydrophobic interactions. The protein inhibits the activity of α-amylase, with inhibition as high as 77.32% at a protein concentration of 80 mg/mL and a semi-inhibitory concentration of 35.22 mg/mL. Thus, the addition of protein attenuates starch digestion. These findings elucidate the mechanism of protein effects on starch digestion properties during fermented barley digestion and provide a theoretical basis for the subsequent preparation of low-glycemic starch foods.

Mechanism of hydrocolloids effect on buckwheat starch gels from interaction and structural perspectives: A comparative study

Controlling the digestibility and gel properties of Tartary buckwheat starch (TBS) has become a central issue for functional foods. The effects of hydroxypropyl methylcellulose (HPMC), guar gum (GG) and Konjac glucomannan (KGM) on TBS from the interaction and structural perspectives were studied. Three hydrocolloids increased the peak, trough and final viscosity of TBS in a concentration-dependent manner. Dynamic frequency sweeps and flow state tests indicated that the TBS–hydrocolloid systems exhibited gel-like behaviour, and TBS-HPMC was more thixotropic than the other systems. The addition of hydrocolloids significantly enhanced the hardness and chewiness of the TBS gels. The structural analyses revealed that hydrocolloids did not affect the functional groups or crystalline structure of TBS, but did improve the orderliness of the systems. The interaction mechanism confirmed that hydrogen bonds and electrostatic forces are the main forces in the formation of HPMC and TBS–KGM gels; while hydrogen bonds are dominant in TBS–GG gels. Moreover, HPMC, GG and KGM can each delay starch hydrolysis to some extent, with a hydrolysis rate varying from 78.66 % ± 2.81 % to 44.8 % ± 0.35 % at three addition levels. The results can provide both theoretical and practical insights into the glycaemic control of starch and TBS-based jelly foods production.

Effect of Ethanol Extract of Tea on the Physicochemical, Retrogradation Characteristics, and Digestion Properties of Wheat Starch

AbstractThe effects of ethanol extract of tea (EET) on the physicochemical properties, microstructure, and digestibility of wheat starch (WS) are investigated. Aging degree, solubility, swelling degree, Springness of gel, thermodynamic properties, X‐ray diffraction, infrared spectroscopy, Scanning electron microscopy (SEM), and digestibility of WS by adding different ratios of EET are analyzed. The changes of EET on WS‐related properties are evaluated comprehensively. The results show that the addition of EET reduces the enthalpy of recovery and inhibits the recovery of WS. At the same time, XRD diffraction shows that the addition of EET reduces the crystallinity of WS. At the same time, the addition of EET also decreases the content of rapidly digestible starch (RDS) in WS and increases the content of slowly digestible starch (SDS) and resistant starch (RS). In conclusion, EET can inhibit the retrogression of WS and can also be used as a potential source of RS additives.

Effect of four highland barley proteins on the retrogradation and in vitro digestion properties of highland barley starch

This study evaluated the effects of four highland barley proteins (HBPs), namely albumin, globulin, gliadin and glutenin, on the retrogradation and in vitro digestion properties of highland barley starch (HBS). The results showed globulin had the most significant effect on inhibiting short-term retrogradation of HBS, which was reflected in the reduction of G' and G". Compare with HBS, four HBPs could significantly inhibit long-term recrystallization process. For albumin, globulin, gliadin and glutenin, the degree of retrogradation reduced from 51.55 % to 48.20 %, 35.06 %, 42.22 % and 32.63 %, respectively, which was reflected in the decrease of water migration rate, crystal enthalpy, crystallinity and short-range order. It could be found that glutenin had the most significant effect on inhibiting long-term retrogradation of HBS. Moreover, the anti-digestion properties of retrograded HBS with HBPs intervention significantly increased, with glutenin most significantly. Compared with HBS, resistant starch (RS) content increased by 59.76 % at 28 d of retrogradation.

The influence of pulse cell wall structure and cellular protein matrix on the in vitro digestion kinetics of starch: A dual encapsulation mechanism

The intrinsic characteristics and extrinsic processing of whole-pulse food modulate the starch digestion rate and extent. This study investigated the dual encapsulation mechanism of cell wall structure and protein matrix on the in vitro digestion properties of intracellular starch, using an isolated whole-pulse food model of intact pea cotyledon cells subjected to alkaline buffer and enzymatic treatments. Results showed that intact cells with the maximum protein matrix content (18.9 %) exhibited the lowest peak temperature (71.4 °C, uncooked and 58.1 °C, cooked), enthalpy change (3.4 J/g, uncooked and 2.0 J/g, cooked), relative crystallinity (11.6 %), and starch digestion rate (0.0248 min−1) and extent (11.9 %) compared to alkaline buffer and enzymatic treatments. Even after enzymatic treatment, cells with minimal protein matrix content (1.8 %) exhibited a starch digestion rate (0.0387 min−1) and extent (39.7 %), which were still lower than those of isolated starch (0.0480 min−1 and 56.8 %). These findings indicate that the protein matrix and cell walls act as a dual encapsulation system to slow starch hydrolysis. This provides a theoretical basis and technical guidance for developing low-glycemic whole-pulse foods.

Physicochemical, Antioxidant, Starch Digestibility, and Sensory Properties of Wheat Bread Fortified with Taiwanese Cocoa Bean Shells.

The effects of replacing 5-25% of wheat flour (WF) with Taiwanese cocoa bean shells (CBSs) on the physicochemical, antioxidant, starch digestion, and sensory properties of the bread were studied. The lead (0.18) and cadmium (0.77) contents (mg/kg) of the CBSs were below the Codex Alimentarius specifications for cocoa powder. Ochratoxin A and aflatoxins (B1, B2, G1, and G2) were not detected in the CBSs. The CBSs were rich in dietary fiber (42.9%) and bioactive components and showed good antioxidant capacity. The ash, fat, protein, dietary fiber, crumb a* and c*, hardness, chewiness, total phenols, and antioxidant activities of the bread increased with an increasing CBSs level. The starch hydrolysis rate (45.1-36.49%) of the CBS breads at 180 min was lower than that of the control (49.6%). The predicted glycemic index of the bread (CBS20 and CBS25) with 20-25% of the WF replaced with CBSs was classified as a medium-GI food using white bread as a reference. In the nine-point hedonic test, the overall preference scores were highest for control (6.8) and CBS breads, where CBSs replaced 5-10% of WF, with scores of 7.2 and 6.7. CBS20 supplemented with an additional 20-30% water improved its volume, specific volume, and staling rate, but the overall liking score (6.5-7.2) was not significantly different from the control (p > 0.05). Overall, partially replacing wheat flour with CBSs in the production of baked bread can result in a new medium-GI value food containing more dietary fiber, bioactive compounds, and enhanced antioxidant capacity.

The Effect of Maltose on Structural, Physicochemical, and Digestive Properties of Lentil Starch under Electron Beam Irradiation.

This study investigated the effects of electron beam irradiation (EBI) on the structural, physicochemical, and functional properties of lentil starch with varying maltose content. EBI did not significantly disrupt the starch's surface structure or cause amorphization of starch and maltose crystals, but it significantly reduced the intensity of starch's XRD peaks. The presence of maltose intensified internal growth ring damage, leading to more cross-link and rearrangement between short chains, improving short-range ordering of lentil starch and enhancing starch's solubility and thermal stability. Additionally, adding maltose that EBI then treats can lead to an increased content of slowly digestible starch in samples.

In situ starch and water modification using atmospheric plasma: Structure, functionality and in vitro digestibility

Starch modification has become necessary to develop functional foods. Sustainable technologies like plasma-activated water (PAW) have been combined with hydrothermal treatments; however, the scaling-up process is challenging. Therefore, a simultaneous treatment of starch and water in a plasma reactor and a 24 h soaking of starch-water after the treatment were compared. The effect of the treatments on the structural, functional, and digestibility properties of starches (normal (NS), waxy (WS), and high amylose (HAS)) with different amylose/amylopectin content was assessed. The results show that simultaneous treatment mainly affected the water properties. Nevertheless, the contact time (24 h) after treatment between starch and water promoted the alteration of amylose chains, branching amylopectin molecules and hydrated crystals of HAS, increasing its rapidly (RDS) and slowly (SDS) digestible starch content. Meanwhile, in NS, the contact time favored the high re-engagement of amylose-amylopectin chains inside starch granules, significantly improving the resistant starch (RS) fraction from 31 % to 75 %.

Effects of cyclodextrin glycosyltransferase on physicochemical, digestion, and gel properties of corn and potato starches

The application of natural starches in the food industry is sometimes limited because of their high viscosity, strong susceptibility to retrogradation, and poor water retention capacity. In this study, cyclodextrin glycosyltransferase (CGTase) was used to modify corn starch (CS) and potato starch (PS) to investigate their physicochemical, digestion and gel properties. Rapid Visco-Analyzer (RVA) was used to determine the impact of this enzyme treatment on the gelatinization and retrogradation behavior of these two starches. The CGTase treatment reduced the viscosity and inhibited the short-term retrogradation of both starches. Enzymatic modification weakened the gel structure of corn starch but enhanced the gel structure of potato starch. The microstructure confirmed the above conclusion. The gel wall of CS became thinner, but more and smaller grids were generated, and the water holding capacity was enhanced after enzyme treatment, while the wall thickness of PS was not significantly changed, and the water holding capacity was relatively good on the whole. Compared with native starch, the enzyme modified starch exhibited a similar digestibility but the content of rapidly digestible starch was slightly higher. The crystal structure of starches was disrupted by the enzyme treatment, but that the covalent bonds within starches were not affected. Starch gels formed by CGTase treatment could be successfully used as edible inks in 3D food printing applications, which may be useful for the creation of personalized foods.

In vitro Protein and Starch Digestibility, Nutritional and Bioactive Properties of Elekute (A Maize-Based Snack) Substituted with Catfish (Clarias gariepinus)

In vitro Protein and Starch Digestibility, Nutritional and Bioactive Properties of Elekute (A Maize-Based Snack) Substituted with Catfish (Clarias gariepinus)

Incorporation of germinated lupin into corn-based extrudates: Focus on starch digestibility, matrix structure and physicochemical properties

The research aimed to assess the effects of incorporating germinated Lupinus angustifolius flour into corn extrudates for different periods (3, 5, and 7 days), focusing on starch digestibility, morphological structure, thermal, and pasting properties. Extrudate with germinated lupinus flour for 7 days (EG7) significantly increased the content of slowly digestible starch up to 10.56% (p < 0.05). Crystallinity increased up to 20% in extrudates with germinated flour compared to extrudates with ungerminated flour (EUG), observing changes at the molecular level by FTIR that impact the thermal and pasting properties. X-ray diffraction revealed angles of 2θ = 11.31, 16.60, 19.91, and 33.04 as a result of the germination and extrusion processes. Microstructural analysis indicated starch-protein interactions influencing changes in calorimetry, viscosity, X-ray diffraction, and digestibility. PCA allowed establishing that the addition of germinated flours significantly affected the properties and microstructural characteristics of extruded products, potentially affecting digestibility and nutritional quality.

Amylopectin content rather than amylose or protein content is critical to determining the starch digestion rate in high-amylose rice

The starch digestion properties of cooked rice are associated with the chemical compositions of the rice grain. However, limited information is available on the chemical composition governing starch digestion properties in high-amylose rice. To overcome this knowledge gap, correlation plot analysis and partial correlation analysis were utilized to assess the correlations between active digestion duration, total glucose production, and glucose production rate of cooked rice to amylose, amylopectin, and protein content of rice grains throughout 17 high-amylose varieties. The correlation plot analysis identified significant positive or negative linkages between total glucose production and the glucose production rate of cooked rice with grain amylose, amylopectin, and protein content. Nevertheless, partial correlation analysis only characterized a significant positive correlation between glucose production rate and grain amylopectin content. The findings of this study indicate that grain amylopectin content rather than grain amylose or protein content is critical for the determination of the rate of starch digestion in high-amylose rice.

Effects of repeated and continuous dry heat treatments on the physicochemical, structural, and in vitro digestion properties of chickpea starch

The study investigated the impacts of repeated (RDH) and continuous dry heat (CDH) treatments on the physicochemical, structural, and in vitro digestion properties of chickpea starch. The results of SEM and CLSM showed that more fissures and holes appeared on the surface of granules as the treated time of CDH and the circles of RDH increased, both of which made the starch sample much easier to break down by digestive enzymes. Moreover, the fissures and holes of starch granules treated by CDH were more obvious than those of RDH. The XRD and FT-IR results suggested that the crystal type remained C-type, and the relative crystallinity and R1047/1022 of the chickpea starch decreased after dry heat treatments. In addition, a marked decline in the pasting viscosity and gelatinization temperature of chickpea starches was found with dry heat treatments. Moreover, the increased enzyme accessibility of starch was fitted as suggested by the increased RDS content and digestion rate. This study provided basic data for the rational design of chickpea starch-based foods with nutritional functions.

Role of particle size in modulating starch digestibility and textural properties in a rye bread model system

In cereal products, the use of flour containing clusters of intact cells has been indicated as a potential strategy to decrease starch digestion. Rye possesses more uniform and thicker cell walls than wheat but its protective effect against starch digestion has not been elucidated. In this study, rye flours with three different particle sizes, large (LF) (∼1700 μm), medium (MF) (∼1200 μm), and small (SF) (∼350 μm), were used to produce model bread. The textural properties of these breads were analysed using Textural Profile Analysis (TPA). The starch digestibility of both the flour and the bread was measured using Englyst’s method, while the presence of intact cell clusters was examined using Confocal Laser Scanning Microscopy (CLSM). Additionally, the disintegration of bread digesta during simulated digestion was assessed through image analysis. CLSM micrographs revealed that bread made with MF and LF retained clusters of intact cells after processing, whereas bread made with SF showed damaged cell walls. Starch digestibility in LF and MF was lower (p ≤ 0.05) than that in SF. Bread produced with MF and LF exhibited the least (p ≤ 0.05) cohesive and resilient texture, disintegrated more during digestion, and exhibited higher starch digestibility (p ≤ 0.05) than bread made with SF. These results highlight the central role of bread texture on in vitro starch digestibility.

Noodles made from blends of rice flour and pineapple stem starch: physical properties, cooking qualities, in vitro starch digestibility and sensory properties

SummaryNoodles are generally made from rice flour, wheat flour or mungbean starch. Interestingly, pineapple stem starch (PSS), an alternative starch derived from pineapple stems as agricultural waste, has not yet been investigated for noodle production. The noodles were then prepared in different ratios of commercial rice flour and PSS (100:0, 70:25, 50:50, 25:75 and 0:100) to investigate the effects of PSS on noodle qualities and properties including physical properties, cooking quality, in vitro starch digestibility and sensory attributes. The results showed that high PSS content had a positive effect on the structure and textural properties of noodles by increasing the network structure, hardness and chewiness, resulting in good sensory quality, which could be due to the high amylose content (30.56%) of PSS. However, the optimal cooking time and the cooking loss of the cooked noodles increased with an increasing proportion of PSS. In addition, noodles with a high PSS content had a higher content of slowly digestible starch and resistant starch, which can have a positive effect on our health. Our results suggest that PSS, an alternative starch from agricultural waste, can replace rice flour to develop better‐quality gluten‐free noodles. Similarly, PSS‐based noodles with a high resistant starch content could benefit human health by lowering the glycemic index and reducing the risk of non‐communicable diseases.

Effect of combined freezing with heat-moisture treatment (HMT) on the modification of in vitro digestibility, morphostructural, physicochemical, and thermal properties of Adzuki bean starch

Effect of combined freezing with heat-moisture treatment (HMT) on the modification of in vitro digestibility, morphostructural, physicochemical, and thermal properties of Adzuki bean starch

Effects of rosemary extract addition on starch bioavailability and antioxidant properties of extruded pet foods

This study aimed to investigate the effects of rosemary extract (RE) addition on the starch digestion and antioxidant properties of extruded pet foods. Pet foods were extruded using rice, round pea (RP), and wrinkled pea (WP) flours as the sole starch sources with RE incorporated at levels of 0 (control), 1.0, 10.0, and 30.0 g/kg (RE0, RE1, RE10, and RE30, respectively). High-amylose WP pet food exhibited lower damaged/gelatinized starch content compared with rice and RP pet foods. The total phenolic contents and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability of the pet foods notably increased after the addition of RE, showing a clear upward trend as the incorporation level was elevated. RE10 (except for rice) and RE30 significantly decreased the starch digestibility of pet foods according to in vitro testing, with the greatest reduction observed for the high-amylose WP sample. Although RE1 did not significantly impact the overall in vitro and in vivo starch digestibility, the treatment consistently postponed the peak times of postprandial blood glucose responses to all the three extruded diets in beagles. The results indicated that RE effectively enhanced the antioxidant properties of extruded pet foods and slowed starch digestion rates.

Novel and Conventional Steam Blanching Impacts on Potato Starch Digestibility and Physicochemical Properties

Novel and Conventional Steam Blanching Impacts on Potato Starch Digestibility and Physicochemical Properties

The role of C18 fatty acids in improving the digestion and retrogradation properties of highland barley starch

In this study, five C18 fatty acids (FA) with different numbers of double bonds and configurations including stearic acid (SA), oleic acid (OA), elaidic acid (EA), linoleic acid (LA), and α-linolenic acid (ALA), were selected to prepare highland barely starch (HBS)-FA complexes to modulate digestibility and elaborate the underlying mechanism. The results showed that HBS-SA had the highest complex index (34.18 %), relative crystallinity (17.62 %) and single helix content (25.78 %). Furthermore, the HBS-C18 FA complexes were formed by EA (C18 FA with monounsaturated bonds) that had the highest R1047/1022 (1.0509) and lowest full width at half-maximum (FWHM, 20.85), suggesting good short-range ordered structure. Moreover, all C18 FAs could form two kinds of V-type complexes with HBS, which can be confirmed by the results of CLSM and DSC measurements, and all of them showed significantly lower digestibility. HBS-EA possessed the highest resistant starch content (20.17 %), while HBS-SA had the highest slowly digestible starch content (26.61 %). In addition, the inhibition of HBS retrogradation by fatty acid addition was further proven, where HBS-SA gel firmness (37.80 g) and aging enthalpy value were the lowest, indicating the most effective. Overall, compounding with fatty acids, especially SA, could be used as a novel way to make functional foods based on HBS.