Nutrient Digestibility Of Diets Research Articles

PSIV-B-28 Effect of feeding acidified or fermented barley grain using Limosilactobacillus reuteri with or without supplemental phytase on diet nutrient digestibility in growing pigs

Abstract Fermentation of cereal grains may degrade myo-inositol hexakisphosphate (InsP6) thereby increasing nutrient digestibility. Effects of chemical acidification or fermentation with Limosilactobacillus (Lm.) reuteri TMW 1.656 with or without phytase on nutrient digestibility of diets composed of high β-glucan hull-less barley grain were assessed in growing pigs. Four mash diets contained 50% barley grain: 1) unfermented barley (Control); 2) chemically-acidified barley (ACD) with lactic and acidic acid [0.02 L/kg barley grain, 4:1 (v/v)]; 3) barley fermented with Lm. reuteri (Fermented without phytase); and 4) barley fermented with Lm. reuteri and phytase (Fermented with phytase; 500 FYT/kg barley grain). The 4 diets were fed to 8 ileal-cannulated barrows (initial BW, 17.4 kg) for four 11-d periods in a double 4 × 4 Latin square. The InsP6 content of barley grain in Control, ACD, Fermented without phytase, or Fermented with phytase was 1.12, 0.59, 0.52% dry matter, or not detectable, respectively. Diet apparent total tract digestibility (ATTD) of crude protein (CP), Ca, gross energy and digestible energy and predicted net energy values were greater (P < 0.05) for ACD and Fermented without phytase than Control. Diet apparent ileal digestibility (AID) of Ca and standardized total tract digestibility (STTD) of P tended to be greater (P < 0.10) for Fermented without phytase than Control. Diet STTD of P, AID and ATTD of Ca was greater (P < 0.05) for Fermented with phytase than Fermented without phytase. Acidification or fermentation with/without phytase did not affect diet standardized ileal digestibility of CP and AA. In conclusion, fermentation with phytase completely degraded InsP6 in barley grain and maximized P and Ca digestibility, thereby reducing the need to provide inorganic P to meet P requirements of growing pigs.

PSIII-4 Nutritional evaluation of yeast biomass from candida utilis as a protein source in cat diets

Abstract Yeast products have found much favor within companion animal nutrition. A recently developed proprietary process has introduced an enhanced yeast biomass from Candida utilis (merchandised as SylPro®) into the animal food marketplace. Candida utilis has been shown to be a valuable protein in canine, swine and aquaculture diets; however, no previous research has evaluated its use in feline diets. Therefore, the current objective was to determine the nutrient digestibility of diets containing SylPro® yeast biomass (SYL) as the primary protein source relative to soybean meal (SOY), pea protein concentrate (PEA), and chicken meal (CKN) fed to cats. Apparent total tract digestibility (ATTD) of diets were estimated using Titanium dioxide as an indigestible marker. The DM ATTD for SYL was lower (P < 0.0001) than CKN (86.39 vs. 87.43%, respectively) but similar to both SOY (85.66%) and PEA (86.54%). The OM ATTD of SYL, SOY, and PEA were not different (P < 0.0001) and were each lower than CKN (average of 89.60 vs. 91.16%, respectively). The CP ATTD for SYL (89.9%) was not different from the other three treatments (P = 0.0200). Crude fat ATTD of SYL was lower (92.52%; P < 0.0001) than all treatments with PEA being greatest (94.82%) and CKN and SOY intermediate (average of 93.84%). The SYL and CKN had higher TDF ATTD (average of 66.20%; P < 0.0001) relative to PEA and SOY (average of 49.79%). The GE ATTD of SYL was lower than CKN (90.18 vs. 90.97%, respectively; P = 0.0154) but similar to both SOY (90.18%) and PEA (90.30%). In conclusion, all diets were highly digestible but CKN preformed the best. The SYL diet was similar to CKN for ATTD of CP and TDF. The SYL diet was also similar to both SOY and PEA for ATTD of DM, OM, CP and GE.

The effect of creep feed composition and form on pre- and post-weaning growth performance of pigs and the utilization of low-complexity nursery diets.

Fifty-six litters from first-parity sows standardized to 12 piglets were used to determine the effects of creep feed composition and form on pre- and post-weaning pig growth performance and the utilization of low-complexity nursery diets. At 5 days of age, litters (initial body weight [BW] 2.31 ± 0.61 kg) were assigned to one of four creep feeding regimens (n = 14): 1) pelleted commercial creep feed (COM), 2) liquid milk replacer (LMR), 3) pelleted milk replacer (PMR), or 4) no creep feed (NO); creep feeds contained 1.0% brilliant blue as a fecal marker. Individual piglet BW and fecal swabs were collected every 3 ± 1 days during the creep-feeding period. The latter was to identify piglets that regularly consumed creep feed via the visual appearance of blue dye in the feces. At weaning (21 ± 2 days of age), six pigs per litter with median BW that consumed creep feed were placed on either a HIGH− (contained highly digestible animal proteins) or LOW− (contained corn and soybean meal as the main protein sources) complexity nursery diet (n = 7) in a three-phase feeding program over 39 days. On day 8, two pigs per pen were sacrificed to collect organ weights and digesta. The LMR disappeared at the greatest rate (average 37.7 g/pig/d; dry matter-basis) versus COM and PMR (10.6 and 10.3 ± 1.5 g/pig/d, respectively; P < 0.001). Litters that received LMR had the greatest proportion of pigs with blue fecal swabs throughout the creep feeding period (85.0 vs. 54.9 and 63.0% ± 0.4% for COM and PMR, respectively; P < 0.05) and LMR piglets had greater BW at weaning versus all other treatments (6.32 vs. 6.02, 5.92, and 5.67 ± 0.14 kg, for LMR, COM, NO, and PMR, respectively; P < 0.001). Overall, pigs given LOW (vs. HIGH) diets in the nursery period had reduced average daily gain (25.1 vs. 27.7 ± 0.4 g/kg BW; P < 0.001), gain:feed (0.75 vs. 0.81 ± 0.02; P < 0.001), and exit BW (21.2 vs. 24.4 ± 0.6 kg; P < 0.001); no carryover effects of creep feeding program were observed. Creep feed regimen had limited effects on nutrient digestibility of nursery diets but the apparent ileal digestibility of organic matter tended to be less at 28 days of age for pigs that received the LOW nursery diet (64.2 vs. 68.8% ± 2.5%; P = 0.076). Providing supplemental nutrition during the suckling period via LMR improved piglet BW at weaning, which did not correspond to improved post-weaning growth performance, regardless of nursery diet complexity.

Air-classified faba bean protein fraction as a substitute to soybean meal in pelleted and extruded broiler diets

ABSTRACT 1. The hypothesis that air-classified faba bean protein fraction (FBP) can replace soybean meal (SBM) in pelleted or extruded broiler diets without adverse effect on performance or nutrient digestibility was tested. 2. At 17 d of age, male broilers were randomly distributed among four dietary treatments consisting of either SBM or FBP (main dietary protein source) and pelleting or extrusion as processing methods. Treatments had 10 replicate pens containing five birds each. 3. Compared to SBM, birds fed FBP had significantly lower feed intake, less weight gain and had poorer feed conversion. 4. Pellet durability was high (above 92%) for all diets. In pelleted diets, FBP was harder than SBM whereas extruded diets had similar hardness. Pelleting increased water stability compared to extrusion. FBP diets were more water stable than the SBM diets. 5. Gizzard content weight was 2.2-fold higher (P = 0.002) for birds given FBP compared to those fed SBM. The weight of the jejunum and ileum with contents was 1.4-fold lower for the FBP diets, and this effect was larger (P < 0.05) for the extruded FBP diet. 6. Birds fed FBP diets had significantly higher nitrogen digestibility in the lower jejunum and ileum compared to those given SBM diets, while the starch digestibility coefficient was above 0.980 in all treatments. 7. The high nutrient digestibility of FBP diets indicates that the poor performance of the FBP group was due to lower feed intake which was not explained by the differences in pellet durability or hardness. The reduced palatability of the FBP and the longer retention of the FBP diets in the upper gut are more likely to have depressed feed intake.

Effect of the inclusión of herbal phosphatidylcholine on palatability, digestibility and metabolisable energy of the diet in dogs

This study aimed to evaluate the palatability, nutrient digestibility, metabolisable energy (ME) and faecal characteristics of diets in dogs fed increasing levels of herbal phosphatidylcholine (herbal mix) versus an unsupplemented diet (with only 377 mg choline provided by 1 kg food) or choline chloride1 (2000 mg choline/kg food) in 40 adult dogs. In experiment 1, a palatability test was conducted to make two pairwise comparisons: 0 versus 200; and 0 versus 400 mg/kg herbal mix. In experiment 2, a digestibility test was performed to evaluate herbal mix at 0, 200, 400 and 800 mg/kg and 2000 mg choline provided by choline chloride. Results from experiment 1 indicated that the dogs preferred diets containing herbal mix to the unsupplemented diet (P<0.05). In experiment 2, nutrient digestibility and faecal characteristics were not influenced by the treatment (P<0.059). The inclusion of 400 mg/kg of herbal mix increased the ME (quadratic effect, P<0.01). In conclusion, the results of this study indicate that the inclusion of a herbal mix rich in phosphatidylcholine (1.6%) and other methylated metabolites at 400 mg/kg can fully replace choline chloride in dog diets.

Effect of Reduced Dietary Copper Levels Sourced from Organic and Nanoparticles Forms on Performance and Nutrient Utilization in Giriraja Birds

Background: Copper (Cu) is an essential trace mineral for growth, production and health of poultry. Due to its poor absorption from inorganic source which is common supplemental form, large amount of Cu is excreted in the excreta. This experiment was conducted to assess the bioavailability of Cu from organic and nano sources at lower level of supplementation to reduce the excretion level without compromising the growth performance of chicken. Methods: A total of 420-day-old Giriraja chicks were randomly assigned to 7 treatment groups having 4 replicates each (15 chicks per replicate). The basal diets of both chick (0-6 weeks) and grower phases (7-10 weeks) were supplemented with 20 and 30 ppm of Cu from inorganic source as control (T1), the groups T2, T3 and T4 supplemented with 100, 75 and 50% of control from organic Cu, T5, T6 and T7 treatment diets supplemented with 75, 50 and 25% of control from Cu nanoparticles, respectively. Result: The grower phase and cumulative body weight gain was significantly (p less than 0.05) higher in T2 compared to T1, T6 and T7 groups, while the feed intake in chick phase was lower in T2, T5 and T7 groups as compared to T1, T3, T4 and T6. The chick phase feed conversion ratio was significantly (p less than 0.05) better in T2 and T5 groups compared to T1, T3, T4, T6 and T7, whereas the cumulative FCR was better in T2 and T5 compared to T6 and T7 groups, while it was not affected during grower phase. The dry matter and crude protein metabolizability were significantly (p less than 0.05) higher in T2 and T5, the organic matter and ether extract metabolizability was higher only in T5. The crude fiber and nitrogen free extract was not affected by the treatment diets. Nitrogen excretion was significantly (p less than 0.05) lower and its retention was higher in T2 and T5 treatment groups but was comparable with control. The Cu excretion through excreta was significantly (p less than 0.05) higher and its retention was lower in control as compared to all dietary treatments. Hence, it was concluded that the Cu supplementation from organic or nanoparticles form can be reduced by 50 and 75% of standard, respectively without compromising the growth performance of birds and nutrient digestibility of diets.

Effects of alternative energy and protein sources on performance, carcass characteristics, and meat quality of feedlot lambs

The aim of this study was to evaluate the effects of replacing ground corn with crude glycerin and soybean meal by cottonseed cake, in association or not, on digestibility and intake of dietary nutrients, performance, carcass characteristics and meat quality of feedlot lambs. Thirty-two non-castrated crossbred 1/2 Santa Inês x 1/2 Dorper lambs, with an average body weight of 18.52 ± 3.24 kg (mean ± standard deviation) and with 3 months of age were distributed in a randomized complete block design, into four treatments and eight replicates per treatment, in a 2 × 2 factorial scheme. Four experimental diets were tested with different sources or combinations of energy and protein ingredients, respectively, in the concentrate: 1) ground corn + soybean meal; 2) ground corn and cottonseed cake; 3) crude glycerin and soybean meal; 4) crude glycerin and cottonseed cake. There was an interaction between the energy and protein sources in ether extract intake and total digestible nutrients digestibility (P < 0.01). There was an interaction between the energy and protein sources in the total weight gain (P = 0.03), hot (P = 0.03) and cold (P = 0.04) carcass weights, and shear force (P = 0.29). The inclusion of crude glycerin decreased (P = 0.01) the dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), and non-fibrous carbohydrates (NFC) intakes, and the apparent digestibilitis of OM, NDF, and NFC (P < 0.05). Crude glycerin decreased the amount of retained nitrogen (P = 0.02), lower final weight (P < 0.01), average daily gain and cold carcass yield. An increase ocurred in the NDF intake (P < 0.05) when lambs were fed diet with cottonseed cake and a decrease (P < 0.01) in the digestibilities of DM, OM, CP and NDF. The replacement of 100% of ground corn by crude glycerin, with low purity, is not recommended in diets for feedlot lambs, because it decreases animal performance. In contrast, the total replacement of soybean meal by cottonseed cake can be used in diet for lambs, without causing damage to the performance and meat quality.

Feed additive based on biologically active substances for fattening steers

The modern agricultural market offers a wide range of products designed for a highly effective and most importantly safe increase in growth indicators, the level of development and the degree of productivity of domestic animals. The leading role in achieving the highest quality result is played by a competent choice of feed additives. The purpose of the research was to study the use of a feed additive based on biologically active substances (BAS) in the fattening of steers. The effect of the feed additive based on a natural, balanced, natural complex of biologically active substances such as vitamins B, C, PP, tryptophan (with oral administration bioavailability reaches 90 %), antioxidants, essential and free amino acids, enzymes and trace element salts on the growth and development of young cattle of Black-and-White breed has been studied. The steers of the control group received the main diet, and the animals of the experimental group in addition to the main diet received the feed additive consisting of vitamins, amino acids, antioxidants, enzymes and trace element salts. It has been found by the studies that the use of the test feed additive based on BAS provides an improvement in the digestibility and assimilation of nutrients in the diet, which contributed to the increase in the average daily live weight gain in the experimental group by 263 g or 25 %, the decrease in feed consumption for 1 kg of gain and the improvement in blood biochemical parameters, such as inorganic phosphorus, AST, ALT, alkaline phosphatase within physiological norms. In order to balance the diets of 9-monthold steers by biologically active substances and improve the physiological and biochemical status, the digestibility of nutrients that contribute to the increase in the average daily weight gain, it is recommended to use the feed additive based on BAS with drinking water.

Effects of highly purified rapeseed protein isolate as fishmeal alternative on nutrient digestibility and growth performance in diets fed to rainbow trout ( Oncorhynchus mykiss )

The effective inclusion of rapeseed protein products as fishmeal alternatives in diets for carnivorous fish is still limited. Previous studies observed restrictions in both nutrient utilization and feed intake. Contents of nitrogen-free extracts (NfE) and anti-nutritive substances (ANFs) were made responsible for the latter mentioned limitations. Consequently, a highly purified rapeseed protein isolate with high protein content and low levels of both NfE and ANF was investigated in this study. In the first experiment, digestibility of the rapeseed protein isolate was determined. In the second experiment, the fishmeal portion (190 g kg-1) of a control diet was gradually replaced by rapeseed protein isolate to 33%, 66% and 100% of digestible protein and energy. Diets were fed twice per day to apparent satiation to triplicate groups of rainbow trout (Oncorhynchus mykiss). After 56 days of feeding, growth performance and health parameters were evaluated. Protein digestibility of the rapeseed protein isolate was 95.2%, and up to 66% of dietary fishmeal could be replaced without significantly affecting growth or health parameters. Total replacement of fishmeal led to significantly reduced feed intake and consequently reduced growth performance.

Potential application and beneficial effects of a marine microalgal biomass produced in a high-rate algal pond (HRAP) in diets of European sea bass, Dicentrarchus labrax

Microalgae have been used as live food in aquatic species. In recent years, the interest in microalgae has considerably increased, thanks to the evolution of production techniques that have identified them as an ecologically attractive aquafeed ingredient. The present study provides the first data about the effects of dietary inclusion of a microalgae consortium grown in a high-rate algal pond system on zootechnical performance, morphometric indices, and dietary nutrient digestibility as well as morphology and functionality of the digestive system of European sea bass, Dicentrarchus labrax. A dietary treatment including a commercial mono-cultured microalgae (Nannochloropsis sp.) biomass was used for comparison. Six hundred and thirty-six European sea bass juveniles (18 ± 0.28 g) were randomly allotted into 12 experimental groups and fed 4 different diets for 10 weeks: a control diet based on fish meal, fish oil, and plant protein sources; a diet including 10% of Nannochloropsis spp. biomass (100 g/kg diet); and two diets including two levels (10% and 20%) of the microalgal consortium (100 and 200 g/kg diet). Even at the highest dietary inclusion level, the microalgal consortium (200 g/kg diet) did not affect feed palatability and fish growth performance. A significant decrease in the apparent digestibility of dry matter, protein, and energy was observed in diets including 10 and 20% of the microalgal consortium, but all fish exhibited a well-preserved intestinal histomorphology. Moreover, dietary inclusion with the microalgal consortium significantly increased the enzymatic activity of maltase, sucrase-isomaltase, and ɤ-glutamil transpeptidase in the distal intestine of the treated European sea bass. Algal consortium grown using fish farm effluents represents an attempt to enhance the utilization of natural biomasses in aquafeeds when used at 10 % as substitute of vegetable ingredients in diet for European sea bass.

Intake, digestibility, nitrogen balance and performance of crossbreed Boer goats fed with diets containing saltbush (Atriplex nummularia L.) and spineless cactus (Opuntia ficus-indica).

Saltbush and spineless cactus are adapted to arid and semiarid regions and used as an important forage resource in ruminants' diet. However, spineless cactus restricts production due to its low crude protein and NDF levels and high salt and oxalate contents, which can limit the digestibility and intake of dietary nutrients. Therefore, this experiment was conducted to evaluate the best ratio levels of saltbush associated with spineless cactus in diets for crossbreed Boer goats through ingestion, water balance, digestibility, nitrogen balance, growth and carcass weight. Twenty-four castrated crossbred Boer (Boer × Brazilian native) goats 6months of age and average body weight of 20.28kg were utilized. The animals were weighed and distributed in randomized blocks in four experimental diets with saltbush of 8.4, 18.8, 31.2 and 48.3%. The addition of saltbush hay promoted quadratic behaviour (P < 0.05) in intake of dry matter and nutrients. There was no effect (P < 0.05) from the addition of saltbush hay on the digestibility of organic matter, total carbohydrates, non-fibrous carbohydrates and neutral detergent fibre, whereas there was an increase in dry matter. The crude protein digestibility behaved quadratically (P = 0.06), where the maximum digestibility was obtained with the addition of 31.2% of hay. The balance and net nitrogen utilization presented a quadratic effect (P < 0.01). Saltbush in rations for goats presented promising results, the growth rate (0.15kg/day) obtained in diet with a lower proportion of saltbush, would satisfy smallholders when considering that this species, associated with spineless cactus, may reduce the use of concentrate feeds; however, the diet with 31.2% of saltbush hay provided the biggest gain in body and carcass weight of the goats.

Comparative study on the nutrient digestibility of diets containing brewer’s yeast products processed by different techniques fed to T-cannulated growing pigs

To investigate the effect of processing techniques on the nutrient digestibility of diets containing whole brewer’s yeast products, i.e., directly dried (BDY); thermal-induced autolyzed (AYC); and fine-ground yeast (GDY) prepared from the same yeast filter mud, 3 diets, each contains one of the 3 products with similar energy and protein concentration, were formulated, respectively. Six growing pigs (bodyweight = 25.1 ± 2.0 kg) fitted with T-cannula at terminal ileum were allotted to a replicated 3 × 3 Latin square with 3 diets and 3 periods. The ileal digesta and feces were collected in each period and the digestibility coefficients were calculated using the concentration of nutrients and marker (Cr2O3) in tested diet, ileal digesta and feces. The results indicated that the processing methods significantly affect the digestion of most of diet nutrients especially in the foregut of pigs. Compared with BDY diet, the digestion of DM, CP, energy, Ca, total non-starch polysaccharides (TNSP), insoluble non-starch polysaccharides (INSP), total and insoluble mannose, insoluble glucose, and total and 12 individual amino acids was significantly increased in GDY diet in the foregut of pigs (P < 0.05). The AYC diet exhibited higher (P < 0.05) digestibility coefficients of CP, energy, INSP, total and insoluble mannose, and total and 15 individual amino acids than that of BDY diet in the foregut of pigs. The effect of processing methods on nutrient digestion in the hindgut of pigs was diminished with increased (P < 0.05) the digestion of OM, energy, TNSP and INSP in GDY diet and energy, TNSP, INSP and insoluble mannose in AYC diet. After properly processed, products prepared from whole yeast cells exhibited high potential in pig feeding as a major protein ingredient.

Effect of feeding acidified or fermented barley using Limosilactobacillus reuteri with or without supplemental phytase on diet nutrient digestibility in growing pigs.

Fermentation of cereal grains may degrade myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate) (InsP6) thereby increasing nutrient digestibility. Effects of chemical acidification or fermentation with Limosilactobacillus (L.) reuteri with or without phytase of high β-glucan hull-less barley grain on apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients and gross energy (GE), standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AAs), and standardized total tract digestibility (STTD) of P were assessed in growing pigs. Pigs were fed four mash barley-based diets balanced for water content: 1) unfermented barley (Control); 2) chemically acidified barley (ACD) with lactic acid and acidic acid (0.019 L/kg barley grain at a ratio of 4:1 [vol/vol]); 3) barley fermented with L. reuteri TMW 1.656 (Fermented without phytase); and 4) barley fermented with L. reuteri TMW 1.656 and phytase (Fermented with phytase; 500 FYT/kg barley grain). The acidification and fermentation treatments occurred for 24 h at 37 °C in a water bath. The four diets were fed to eight ileal-cannulated barrows (initial body weight [BW], 17.4 kg) for four 11-d periods in a double 4 × 4 Latin square. Barley grain InsP6 content of Control, ACD, Fermented without phytase, or Fermented with phytase was 1.12%, 0.59%, 0.52% dry matter (DM), or not detectable, respectively. Diet ATTD of DM, CP, Ca, and GE, digestible energy (DE), predicted net energy (NE) value, and urinary excretion of P were greater (P < 0.05) for ACD than Control. Diet ATTD of DM, CP, Ca, GE, DE and predicted NE value, urinary excretion of P was greater (P < 0.05), and diet AID of Ca and ATTD and STTD of P tended to be greater (P < 0.10) for Fermented without phytase than Control. Diet ATTD of GE was lower (P < 0.05) and diet ATTD and STTD of P, AID and ATTD of Ca was greater (P < 0.05) for Fermented with phytase than Fermented without phytase. Acidification or fermentation with/without phytase did not affect diet SID of CP and AA. In conclusion, ACD or Fermented without phytase partially degraded InsP6 in barley grain and increased diet ATTD of DM, CP, and GE, but not SID of CP and most AA in growing pigs. Fermentation with phytase entirely degraded InsP6 in barley grain and maximized P and Ca digestibility, thereby reducing the need to provide inorganic dietary P to meet P requirements of growing pigs.

PSIII-17 Extrusion Enhances Nutrient and Energy Digestibility of Pulse Grain-based Diets Fed to Growing Pigs

Abstract Heat processing may enhance nutrient digestibility of diets based on pulse grains. The objective of this study was to determine nutrient and energy digestibility of extruded lentil-based diets containing either supplemental plant or animal protein source in growing pigs. Two diets were formulated to provide 2.4 Mcal NE/kg and 4.35 g standardized ileal digestible Lys/Mcal NE: 1) soybean meal diet (SBM), containing 50% lentil, 31% wheat, and 12.8% soybean meal; and 2) fish meal diet (FM), containing 40% lentil, 45% wheat, and 10% fish meal. Following mixing, each diet was divided into 2 parts: one part remained mash while the other part was extruded using a single-screw extruder (105°C, 400 rpm, 5 kg water/h). The 2 extruded and 2 non-extruded diets were tested in a 2 × 2 factorial arrangement. Eight ileal-cannulated barrows (32.3 kg) were fed the 4 diets at 2.8 times maintenance DE (110 kcal per kg of BW0.75) for four 9-d periods in a double 4 × 4 Latin square. The FM diets had greater (P &amp;lt; 0.05) AID of DM, GE, and most AA, and ATTD of CP, but lower apparent hindgut fermentation of DM and GE than the SBM diets. The AID of CP and AA were 3.2 and 4.7%-units greater (P &amp;lt; 0.05), respectively, and the ATTD of GE and DE values were 2.1 and 3.1%-units greater (P &amp;lt; 0.05), respectively, for the extruded diets than the non-extruded diets. Interactions between protein source and extrusion were not observed. In conclusion, FM diets had greater ileal digestibility of DM, energy, and AA than SBM diets. Extrusion increased the AID of CP and most AA, and DE value of both plant- and animal-protein diets based on lentil grain, indicating that extrusion can increase the energy and protein value of pulse-grain based diets fed to growing pigs.

PSIII-18 Super Dose Phytase and Carbohydrase Cocktail Enhance Ileal Nutrient and Energy Digestibility of Corn-soybean Diets in Nursery Pigs

Abstract Feed enzymes may ameliorate reduced nutrient and energy digestibility in nursery pigs. The objective was to test effects of super-dosing phytase and fiber-degrading enzymes on digestibility of DM, GE, CP, AA, and Ca. We tested supplementing a super dose (added 1,500 FYT/kg) of phytase (Ronozyme Hi-Phos) with or without carbohydrase cocktail that contained 85 FXU β-xylanase/kg, 587 U/g endo-1,4-β-glucanase, 513 U/g endo-1,3(4)-β-glucanase, 15,000 U/g hemicellulases, and 3,000 U/g pectinases in corn-soybean meal diets in a 2 × 2 factorial arrangement. Diets included 68% corn, 17% SBM, and a basal level of 500 FTU/kg of phytase, and were formulated to contain 2.50 Mcal/kg NE and 5.10 gSID Lys/Mcal NE. Eight ileal-cannulated nursery pigs (initial BW 10 kg) were fed 4 diets at 3.0 × maintenance DE (110 kcal per kg of BW0.75) for four 9-day periods in a double 4 × 4 Latin square. Apparent hindgut fermentation (AHF) was calculated as apparent total tract digestibility (ATTD) minus apparent ileal digestibility (AID). Interactions between super-dosing phytase and carbohydrase cocktail were observed. Supplementing either carbohydrase cocktail or super dose phytase, but not their combination, increased (P &amp;lt; 0.05) diet AID of DM, GE, CP, and most AA by 4–5%-units. Supplementing super dose phytase increased (P &amp;lt; 0.05) AID of P by 16%-units and ATTD of P by 10%-units. Supplementing super dose phytase or carbohydrase cocktail did not affect AID of Ca and ATTD of GE, CP, and Ca, and diet DE value. Supplementing carbohydrase cocktail without super dose phytase decreased (P &amp;lt; 0.05) diet AHF of DM, GE, and CP. In conclusion, dietary inclusion of super dose phytase or carbohydrase cocktail increased ileal digestibility of nutrients in nursery pigs, and thereby reduced protein entering the large intestine. Additive or synergistic effects of carbohydrase cocktail and super dose phytase were not detected.

Nutrient digestibility of extruded canola meal in ileal-cannulated growing pigs and effects of its feeding on diet nutrient digestibility and growth performance in weaned pigs

Canola meal (CM) contains less crude protein (CP) and more fiber and anti-nutritional factors such as glucosinolates than soybean meal (SBM) and consequently has a lower nutrient digestibility. Therefore, processing strategies that may increase the feeding value of CM warrant study. In two experiments, the effects of extrusion of Brassica napus CM on apparent (AID) and standardized ileal digestibility (SID) of amino acids (AA), apparent total tract digestibility (ATTD) of gross energy (GE) in growing pigs, and growth performance and diet digestibility in weaned pigs were assessed. Solvent-extracted CM was extruded using a single-screw extruder at three screw speeds: 250 (CM-250), 350 (CM-350), or 450 (CM-450) rpm. In exp. 1, in a double 4 × 4 Latin square, eight ileal-cannulated barrows (initial body weight [BW], 68.1 kg) were fed corn starch-based diets containing 50% CM or extruded CM. The CM sample contained 43.2% CP, 33.2% total dietary fiber (TDF), and 8.9 µmol of total glucosinolates/g on a dry matter (DM) basis. Extrusion increased (P < 0.05) the AID of CP, reduced (P < 0.05) apparent hindgut fermentation of CP, and decreased (P < 0.05) predicted net energy (NE) value of diets. Extrusion increased diet AID and CM SID of most indispensable AA by 3.1 to 5.3%-units. In exp. 2, 200 weaned pigs (initial BW, 8.3 kg) were fed diets containing 20% SBM, CM, or extruded CM starting 2 wk postweaning for 3 wk. The CM sample contained 42.7% CP, 28.3% TDF, and 5.3 µmol total glucosinolates/g DM. Wheat-based diets provided 2.3 Mcal NE/kg and 5.1 g SID Lys/Mcal NE. Dietary inclusion of extruded CM replacing SBM decreased (P < 0.05) diet ATTD of DM, GE and CP, and DE value. Average daily feed intake, average daily gain (ADG), and gain:feed (G:F) of pigs did not differ between extruded CM and SBM diets and were not affected by extrusion, but increasing extruder screw speed linearly increased (P < 0.05) ADG for day 1 to 7 and G:F for the entire trial. In conclusion, extrusion increased diet AID and CM SID of AA but not DE and predicted NE values of CM. However, increasing extruder speed did not further increase the SID of most of the AA of CM in growing pigs. Dietary inclusion of 20% CM or extruded CM did not affect the growth performance in weaned pigs.

Influence of feed additives «Tetra+» and «Glimalask» on the meat productivity of pigs and consumer properties of pork

Currently, the existing literature sources on the use of feed additives “Tetra+” and “Glimalask” do not fully reflect the data on the comparative study of their infl uence on the physiological state, meat productivity and pork quality of fattened young pigs. Under the conditions of the Lower Volga region comprehensive researches were carried out in a comparative aspect to improve meat productivity and pork quality through the use of feed additives “Tetra+” and “Glimalask” in the diets of young pigs on fattening. The purpose of the work was to increase the productive and pork quality of young pigs on fattening due to the use of feed additives “Tetra+” and “Glimalask” in their diets. The research was carried out in the period from 2012 to 2019. The experimental part of the work took place under the conditions of the stud farm named after Lenin in the Surovikinsky district in the Volgograd region. The object of research was young pigs of Large White breed. The positive influence of feed additives on the digestibility and use of nutrients in diets, meat productivity and pork quality, physiological indicators and economic effectiveness of pork production has been proven. It has been found that the level of profi tability of pork production was higher in the experimental groups in comparison with the control group by 5,03 and 4,48 %, respectively. At the same time the highest effectiveness has been obtained from the animals of the 1st experimental group, which received the feed additive “Tetra+” in the diet, which allowed to increase the live weight gain by 6,74 %, the level of profitability by 5,03 %. Thus, the feed additive “Tetra+” was the most effective.

Evaluation of a-amylase and protease on diet nutrient intake and digestibility, and physiological parameters in lambs

The effects of adding exogenous enzymes on the intake and digestibility of dry matter (DM) and on nutrients, and several blood parameters were determined in lambs fed a basal diet of 34% ground corn, 40% tropical grass hay, and 26% soybean meal providing 21% dietary starch. Twelve crossbred lambs (22 kg) were assigned to one of four diets: no additives (1); diets containing α-amylase (2); protease (3); or their combination (4). Diets (DM basis) were offered daily at 4% of animal BW in four 28-day experimental periods each consisting of 21 d of adaptation to the diet followed by 7 d of complete fecal collection. In each period, feed offered, orts and feces were collected, quantified and analyzed for contents of DM, starch, crude protein (CP) and neutral detergent fiber (NDF) to determine intake and digestibility. At the end of each experimental period, blood samples were collected from the individual lambs to determine the concentration of glucose, beta-hidroxy butyrate (BHB), nonesterified fatty acids (NEFA), and insulin. Data were analyzed according to a 4 x 4 Latin Square experimental design. Dietary treatment contrasts were performed using least square means adjustment for multiple comparisons (Tukey-Kramer) as follows: enzymes versus no enzymes, amylase versus no amylase and protease versus no protease. Dry matter intake was similar across treatments (1,106; 1,088; 1,105 and 1,088 g/d for control, and diets containing α-amylase, experimental protease or their combination, respectively). Adding protease to the diet decreased (P&lt;0.05) starch consumption (248 vs. 255 g/d) and increased, but not significantly, NDF digestibility (48.6 vs. 46.8%) as compared to that of lambs fed without the experimental enzyme. Adding enzymes to the diet tended (P&lt;0.10) to decrease blood BHB concentration below the control level (4.26 vs. 4.68 mg/dL). Blood NEFA concentration tended to increase (P&lt;0.10) in lambs fed α-amylase compared to those fed without α-amylase (0.17 vs. 0.14 mEq/L). The insulin level tended (P&lt;0.10) to increase in lambs fed protease versus lambs not receiving the enzyme (80.3 vs. 71.8 pmol/L). Glucose levels were similar for all treatments. Both exogenous enzymes influenced blood metabolites; however, a greater effect was observed in lambs fed the protease.

Effects of fibre source on nutrient digestibility of diets for finishing lambs

Inexpensive fibre sources might be used as an alternative to lucerne hay in diets for finishing lambs. Thus, effects of providing fibre from lucerne hay (LH), soyhulls (SH), maize stover (MS) and Eragrostis teff hay (ET) on the nutrient digestibility of diets of finishing lambs with comparable NDF and nutrient concentration were evaluated. Thirty-six individually housed Merino ram lambs (x̄ = 43.03, SD = 3.72 kg) were randomly allocated to four diets (n = 9 lambs/treatment). A digestibility study was conducted to determine the nutrient availability of these diets. Dry matter (DM) intake of SH (1436 g) was lower than ET (1716 g). No differences were recorded between treatments for digestibility of NDF (0.32 - 0.34), acid detergent fibre (ADF) (0.41 - 0.44), and ether extract (EE) (0.67 - 0.75), except that MS (0.23) had lower NDF digestibility. Digestibility of DM, organic matter (OM) and non-structural carbohydrates (NSC) were similar for the LH (0.72; 0.75; 0.96) and SH (0.70; 0.74; 0.95) diets, whereas digestibility of crude protein (CP) (0.71 vs. 0.68), metabolizable energy (ME) (9.49 vs. 8.90 MJ/kg DM), and the available ash fraction (0.39 vs. 0.28) were altered. The SH and ET (8.70 MJ/kg DM) treatments had similar ME concentrations, but ET had lower DM digestibility (0.68). The MS treatment had the lowest ME concentration (8.25 MJ/kg DM). The LH treatment resulted in overall better nutrient availability compared with SH, MS, and ET.

Use of insect products in pig diets

This review is focusing on effects of inclusion of insect products in pig diets on digestibility, performance, product quality, and health parameters. In 2019 pig feed accounts for 23% of the global feed production. Soybean meal is the most common protein source in pig diets. A shift towards more sustainable feed ingredients can improve the sustainability of entire pig production. Novel protein sources currently evaluated in diets for piglets and growing pigs are insect-based ingredients. Insects are able to convert organic biomass into high-quality protein. Currently the use of insects as protein source in pig diets is not allowed due to transmissible spongiform encephalopathies regulation but it is expected that this will be allowed in the near future. Research efforts on effects of inclusion of insect products on nutrient digestibility, growth performance, product quality and pig health are therefore increasing. Nutrient digestibility of evaluated insect proteins was comparable with traditional protein sources. Nutrient digestibility of insect-based diets as well as effects on growth performance in pigs fed insect-based diets differed between studies. The differences in responses are mainly due to changes in diet ingredients and nutrient composition when insect products are included. Health related parameters were not affected by dietary inclusion of insect products. In general it can be stated that differences in results between studies may be due to different insect species and life stages being used, differences in nutritional value of the insect products, in dietary inclusion levels, in processing techniques applied, effects on palatability of the diet, (weaning) age of the animals involved and research methods applied. Overall, insect products seem to be a good alternative to partly replace traditional protein-rich ingredients in pig diets without adversely affecting growth performance, product quality and health, but more standardised research is required to reduce differences between studies.