Digestion Of Fraction Research Articles

Alpine Diet in Valmalenco (Lombardy, Italy): Nutritional Features of Spontaneous Plants and Traditional Dishes.

Along the Alps, the Alpine diet is considered to be one of the most common nutritional models. Next to traditional animal-based products, spontaneous plants of the territory are collected and eaten. The aim of this study is to evaluate the nutritional features of selected autochthonous plants of the territory and the typical recipe of green gnocchi. The analyses of proximate composition, carotenoid, total phenol, and mineral contents in raw and cooked plant samples and the chemical composition and in vitro starch digestibility in green and control gnocchi were performed. Except for Aruncus dioicus, all the wild plants contained high levels of carotenoids (15-20 mg/100 g FW), mainly as xanthophylls. Rumex acetosa showed the highest levels of total phenols (554 mg GAE/100 g FW), and Urtica dioica can be considered to be a good dietary source of iron, calcium, and magnesium (4.9, 410, and 72 mg/100 g FW). Cooking significantly decreased the potassium and magnesium contents in all wild species, and total phenols and carotenoids in Aruncus dioicus, Blitum bonus-henricus, and Silene vulgaris (p < 0.05). The slowly digestible fraction of starch (%SDS/available starch), which is inversely correlated to insulin demand, was significantly increased in green gnocchi compared to matched control gnocchi (p < 0.05). Traditional consumption of spontaneous plants in the Alpine regions might increase the dietary intakes of several bioactive substances and contribute to cover the nutritional needs of micronutrients.

Non-conventional starches isolated from agronomic-improved beans (Phaseolus vulgaris L.): A study of their structure and physicochemical properties.

Non-conventional starch sources are promising alternative food ingredients. Different bean varieties with agronomic improvements are constantly being developed and cultivated in the North-western Argentinean region (NOA) to increase yields and obtain high-quality seeds. However, the main attributes of their starches have not been studied. In this work, starches from 4 agronomic-improved bean cultivars were isolated and their structure and physicochemical properties were evaluated. High-purity starches were obtained, as their low protein and ash contents showed. Starch granules presented smooth surfaces with spherical to oval shapes, with a marked "maltese cross" and heterogeneous sizes. Their amylose content revealed a mean value of 318 g.kg-1 and all presented resistant > slowly digestible > rapidly digestible starch fractions. Their FTIR spectra were almost similar and the X-ray diffraction analysis showed a CA- type pattern in all cases despite their different sources. Among thermal properties, Escarlata´ starch showed the lowest gelatinization peak temperature (69.5 °C) and Anahí´ starch the highest (71.3 °C). Starch´s pasting temperatures varied from 74.6 to 76.9 °C, whereas peak viscosity and final viscosity had a similar tendency, being Leales B30 < Anahí < Escarlata < Cegro 99/11-2 and Leales B30 < Anahí = Escarlata < Cegro 99/11-2, respectively. This study provides a basis for a better understanding of the characteristics of agronomic-improved NOA bean starches, enabling their use in product formulation as an alternative to starches from conventional sources. This article is protected by copyright. All rights reserved.

Bioconversion of Carrot Pomace to Value-Added Products: Rhizopus delemar Fungal Biomass and Cellulose

Carrot pomace (CP) which is generated in a large volume in the juice production process, is rich in cellulose, hemicellulose, sugars, pectin, and minerals. However, in many previous investigations, only cellulose was purified and utilized while other components of CP were discarded as waste. Here, CP was valorized into fungal biomass and cellulose with the aim of utilizing all the CP components. Enzymatic pretreatments were applied to solubilize the digestible fraction of CP including hemicellulose, pectin, sucrose, and other sugars for fungal cultivation, while cellulose remained intact in the solid fraction. The dissolved fraction was utilized as a substrate for the cultivation of an edible fungus (Rhizopus delemar). Fungal cultivation was performed in shake flasks and bench-scale bioreactors. The highest fungal biomass concentration was obtained after pretreatment with invertase (5.01 g/L) after 72 h of cultivation (36 and 42% higher than the concentrations obtained after hemicellulase and pectinase treatments, respectively). Invertase pretreatment resulted in the hydrolysis of sucrose, which could then be taken up by the fungus. Carbohydrate analysis showed 28–33% glucan, 4.1–4.9% other polysaccharides, 0.01% lignin, and 2.7–7% ash in the CP residues after enzymatic pretreatment. Fourier transform infrared spectroscopy and thermogravimetric analysis also confirmed the presence of cellulose in this fraction. The obtained fungal biomass has a high potential for food or feed applications, or as a raw material for the development of biomaterials. Cellulose could be purified from the solid fraction and used for applications such as biobased-textiles or membranes for wastewater treatment, where pure cellulose is needed.

Treatment of human waste in small-scale facilities: a prospective review

A sustainable approach to the small-scale treatment of portable toilet and septic tank waste is in need to minimise the associated public health and environmental risks. Treatment of human wastes is highly regulated by legislation in many countries given the high content of organic components and pathogens. In conventional wastewater treatment plants, sewage undergoes a series of treatment stages (primary, secondary and tertiary) before effluent discharge. Delivering this approach on a small scale, to meet demands of temporary events, public gatherings or remote locations provides benefits in reducing transport and bulk handling. For a small-scale treatment process to effectively work, the process steps need to be simplified and minimised. The potential of a new treatment scheme is reviewed where the first step is the solid–liquid fraction separation, followed by anaerobic digestion (AD) of the solid fraction, including energy recovery in a combined heat and power unit. The liquid fraction undergoes a series of filtration and disinfection steps to comply with effluent regulations. Digestate from AD is burned on site to provide local domestic/office heating. This has a great potential for application in different locations where inputs may be sporadic, such as outdoor festivals, disaster response scenarios and construction sites.

An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers.

Fermentation of protein in the caeca of chickens may lead to the production of potentially detrimental metabolites, which can reduce gut health. A poor precaecal digestion is expected to increase protein fermentation (PF), as more proteins are likely to enter the caeca. It is unknown if the undigested protein that enters the caeca differs in fermentability depending on their ingredient source. In order to predict which feed ingredients increase the risk of PF, an in vitro procedure was developed, which simulates the gastric and enteric digestion, subsequent caecal fermentation. After digestion, amino acids and peptides smaller than 3.5 kD in the soluble fraction were removed by means of dialysis. These amino acids and peptides are assumed to be hydrolysed and absorbed in the small intestine of poultry and therefore not used in the fermentation assay. The remaining soluble and fine digesta fractions were inoculated with caecal microbes. In chicken, the soluble and fine fractions enter the caeca, to be fermented, while insoluble and coarse fractions bypass them. The inoculum was made N-free to ensure bacteria would require the N from the digesta fractions for their growth and activity. The gas production (GP) from the inoculum, therefore, reflected the ability of bacteria to use N from substrates and was an indirect measure for PF. The Maximum GP rate of ingredients averaged 21.3±0.9ml/h (mean±SEM) and was in some cases more rapid than the positive control (urea, maximum GP rate=16.5ml/h). Only small differences in GP kinetics were found between protein ingredients. Branched-chain fatty acids and ammonia concentrations in the fermentation fluid after 24 hours showed no differences between ingredients. Results indicate that solubilised undigested proteins larger than 3.5 kD are rapidly fermented independent of its source when an equal amount of N is present.

Sustainable Management of Anaerobic Digestate: From Biogas Plant to Full-Scale Cultivation of Pleurotus ostreatus

In the last decades, the number of studies about the valorization of agricultural by-products has strongly increased due to the growing focus on the circular economy. In this context, mushroom cultivation is raising attention for its nutritional properties and adaptability to different growing conditions. The goal of this project is, therefore, to create a link between two production chains, valorizing the solid anaerobic digestate fraction from the biogas plant as a potential substrate for Pleurotus ostreatus full-scale cultivation. A preliminary in vitro trial shows that the mycelia can colonize at the same rate in both the conventional substrate, which was wheat straw added with sunflower cake and the experimental ones supplemented with corn digestate. The most important and innovative part of the work was full-scale cultivation. Quantitative analyses demonstrate that P. ostreatus is capable of growing on the conventional substrate as well as on the substrate containing 15% of digestate without reducing the mushroom harvest. Even better, digestate seems to stimulate a precocious and more homogeneous production. Chemical and spectroscopic analyses on the fruiting bodies confirm the results; in fact, mushrooms show a comparable structural composition, highlighted with FTIR and SERS spectroscopic evaluations. Moreover, the nutrient content did not change due to the addition of digestate. These promising results demonstrate that anaerobic digestate can become a resource for P. ostreatus cultivation without production losses and maintaining the same qualitative characteristics.

Rumen Biogeographical Regions and Microbiome Variation.

The rumen is a complex organ that is critical for its host to convert low-quality feedstuffs into energy. The conversion of lignocellulosic biomass to volatile fatty acids and other end products is primarily driven by the rumen microbiome and its interaction with the host. Importantly, the rumen is demarcated into five distinct rumen sacs as a result of anatomical structure, resulting in variable physiology among the sacs. However, rumen nutritional and microbiome studies have historically focused on the bulk content or fluids sampled from single regions within the rumen. Examining the rumen microbiome from only one or two biogeographical regions is likely not sufficient to provide a comprehensive analysis of the rumen microbiome and its fermentative capacity. Rumen biogeography, digesta fraction, and microbial rumen-tissue association all impact the diversity and function of the entirety of the rumen microbiome. Therefore, this review discusses the importance of the rumen biographical regions and their contribution to microbiome variation.

A farm-scale sustainability assessment of the anaerobic digestate application methods

Digestate is the anaerobic digestion by-product that can be used as an organic fertilizer, but some agronomic and environmental concerns still hinder its application on land. This study aims to evaluate the agronomic performances (i.e., silage maize dry yield, protein content, and N uptake) and environmental sustainability of solid and liquid digestate fraction application in a field experiment involving two farms with different textures covering about 47 ha in North Italy. The best available distribution methods included mineral fertilizer (MF), mineral fertilizer in variable rate application (VRA) (VRA-MF), liquid digestate with a nitrification inhibitor (LD+), liquid digestate in VRA (VRA-LD), liquid digestate with a nitrification inhibitor in VRA (VRA-LD+), and solid digestate (SD) and were applied to silage maize (Zea mays L.) in 2019 and 2020 cropping seasons. Results showed that both digestate fractions gave satisfying agronomic performances (i.e., dry biomass > 13 t ha-1 and protein content > 6.8%), comparable to those of mineral fertilizers, irrespective of soil type and application techniques. On the contrary, system sustainability investigated with a spatial evaluation of nitrogen use efficiency (NUE) revealed a strong interaction between NUE and soil texture. Indeed, in fine-textured soil only the adoption of both VRA and the nitrification inhibitor allowed the liquid digestate to reach a NUE between 50% and 90% while SD exhibited poor NUE (e.g., < 50%). In conclusion, liquid digestate fraction might be an effective substitute for mineral fertilizers in silty soils meeting also environmental criteria when VRA or nitrification inhibitors are applied. Contrarily, longer-term experiments are requested to evaluate SD fraction sustainability.

Dynamic Simulation and Thermoeconomic Analysis of a Novel Hybrid Solar System for Biomethane Production by the Organic Fraction of Municipal Wastes

The anaerobic digestion of the organic fraction of municipal solid waste and the biogas production obtained from its stabilization are becoming an increasingly attractive solution, due to their beneficial effects on the environment. In this way, the waste is considered a resource allowing a reduction in the quantity of it going to landfills and the derived greenhouse gas emissions. Simultaneously, the upgrading process of biogas into biomethane can address the issues dealing with decarbonization of the transport. In this work, the production of biogas obtained from the organic fraction of municipal solid wastes in a plug flow reactor is analyzed. In order to steer the chemical reactions, the temperature of the process must be kept under control. A new simulation model, implemented in the MatLab® environment, is developed to predict the temperature field within the reactor, in order to assess how the temperature affects the growth and the decay of the main microbial species. A thermal model, based on two equilibrium equations, is implemented to describe the heat transfer between the digester and the environment and between the digester and the internal heat exchanger. A biological model, based on suitable differential equations, is also included for the calculation of the biological processes occurring in the reactor. The proposed anaerobic digestion model is derived by the combination of these two models, and it is able to simultaneously simulate both thermal and biological processes occurring within the reactor. In addition to the thermal energy demand, the plant requires huge amounts of electricity due to the presence of a biogas upgrading process, converting biogas into biomethane. Therefore, the in-house developed model is integrated into a TRNSYS environment, to perform a yearly dynamic simulation of the reactor in combination with other renewable technologies. In the developed system layout, the thermal energy required to control the temperature of the reactor is matched by a solar thermal source. The electrical demand is met by the means of a photovoltaic field. In this work, a detailed thermoeconomic analysis is also proposed to compare the environmental impact and economic feasibility of a biomethane production plant based on a plug flow reactor and fed by renewables. Several economic incentives are considered and compared to determine the optimal solution, both in terms of energy and economic savings. The plant is designed for the treatment of a waste flow rate equal to 626.4 kg/h, and the biomethane produced, approximately 850 tons/years, is injected into the national gas grid or supplied to gas stations. In the proposed plant, a solar field of an evacuated tube collector having a surface of approximately 200 m2 is able to satisfy 35% of the thermal energy demand while over 50% of the electric demand is met with a photovoltaic field of 400 m2. A promising payback time of approximately 5 years was estimated.

Acidification and solar drying of manure-based digestate to produce improved fertilizing products

The increase in energy and fertilizer consumption makes it necessary to develop sustainable alternatives for agriculture. Anaerobic digestion and digestates appeared to be suitable options. However, untreated digestates still have high water content and can increase greenhouse gas emissions during storage and land application. In this study, manure-derived digestate and solid fraction of digestate after separation were treated with a novel solar drying technology to reduce their water content, combined with acidification to reduce the gaseous emissions. The acidified digestate and acidified solid fraction of digestate recovered more nitrogen and ammonia nitrogen than their respective non-acidified products (1.5–1.3 times for TN; 14 times for TAN). Ammonia and methane emissions were reduced up to 94% and 72% respectively, compared to the non-acidified ones, while N2O increased more than 3 times. Dried digestate and dried acidified digestate can be labeled as NPK organic fertilizer regarding the European regulation, and the dried solid fraction and the improved dried acidified solid fraction can be labeled as N or P organic fertilizer. Moreover, plant tests showed that N concentrations in fresh lettuce leaves were within the EU limit with all products in all the cases. However, zinc concentration appeared to be a limitation in some of the products as their concentration exceeded the European legal limits.

The effect of fertilization with microfiltered liquid digestate on the quality parameters of Citrus fruits

Nowadays, the adoption of sustainable agricultural practices, including the reduction of synthetic fertilizers, has become a challenge for the agriculture sector. In this experimental work, the effect of the liquid fraction of digestate (by-product of the anaerobic digestion process) as a fertilizer was evaluated. The aim of the research was to verify to which extent digestate can affect growth and quality parameters of orange fruits, comparing the results to those obtained for fruits grown on soil treated with conventional mineral fertilizers. To assess the effectiveness of the treatments, different qualitative and quantitative parameters of Citrus fruits were measured. In particular, the results showed slight differences between the two treatments, suggesting that digestate may be used for the production of high-quality fruits. Moreover, in some orchards, the Citrus fruits of the plants treated with digestate showed a higher concentration of health-promoting compounds, such as vitamin C, flavonoids, phenolic content, when compared to the control group. Thus, digestate can be considered an optimal source of plant nutrients and can be used as a crop growth promoter, since it represents an effective strategy for reducing the mineral fertilizers input.

Positive effect of Ca addition on the risk of Cu and Zn in digestate as biofertilizer

The addition of Ca in anaerobic digestion will passivate the heavy metals in digestate. The effects of CaCl2, Ca(OH)2 and CaCO3 addition on the anaerobic digestion process and the speciation changes of Cu and Zn in solid fraction of anaerobic digestate (SFD) after digestion were studied. Then the effects of Ca-containing solid fraction digestate (SFD-Ca) application on the speciation changes of Cu and Zn in soil and the effects of ryegrass on the enrichment of Cu and Zn were also explored through pot experiments. The results indicated that: The addition of Ca(OH)2 was beneficial to the transfer of Cu and Zn from the liquid fraction of anaerobic digestate (LFD) to SFD, and the transfer effect was the best. The transfer amount of Cu and Zn increased by about 10.0% and 4.2%, respectively, compared with the blank group. The SFD-Ca promoted the increase of biomass, inhibited the enrichment of Cu and Zn by ryegrass, and the effect of a high concentration of Ca(OH)2 was the best. Ryegrass biomass increased by 19.7%, and the enrichment of Cu and Zn decreased by 39.3% and 31.1%, respectively. High concentration of Ca(OH)2-containing solid fraction digestate (SFD-Ca(OH)2) had the best passivation effect on Cu and Zn in soil, the residual state of Cu and Zn in soil increased by 4.3% and 7.8%, respectively. Adding a high concentration of Ca(OH)2 had a positive effect on ryegrass biomass and reduced the risk of Cu and Zn when SFD was used as biofertilizer.

Effect of Dietary Supplementation of Ginger and Garlic Mixture on Performance of Ardi Goats under Stressful Conditions

Background: Among the different range animals, goats can adapt and survive greatly under harsh environmental conditions. The aim of this study was to investigate the effect of using a mixture of garlic and ginger as supplementation to improve the growth performance and nutrient digestibility of Ardi goats. Methods: A total of twenty Ardi goats (40.35±1.8 kg) were assigned randomly into four experimental groups. The first group received a basal diet (control), the second to fourth groups were dietary supplemented with a mixture of garlic and ginger at the different levels of 20, 40, and 60 g/animal/day, respectively for 12 weeks. Result: There were no significant differences in the final body weight and feed conversion ratio of goats fed different levels of dietary supplemented of ginger and garlic mixture. The TDMI and daily DMI significantly reduced in groups fed diets supplemented with 20 and 40 g/animal/day than the control group. The digestion coefficients of CP, fiber fraction digestibility, and TDN values decreased in different groups. In conclusion, the addition of ginger and garlic mixture at the studied levels did not significantly affect animal performance and had negative impacts on feed utilization and nutrient digestibility.

Geochemical mapping by unmixing alluvial sediments: An example from northern Australia

Alluvial sediments have long been used in geochemical surveys as their compositions are assumed to be representative of areas upstream. Overbank and floodplain sediments, in particular, are increasingly used for regional to continental-scale geochemical mapping. However, during downstream transport, sediments from heterogeneous source regions are carried away from their source regions and mixed. Consequently, using alluvial sedimentary geochemical data to generate continuous geochemical maps remains challenging. In this study we demonstrate a technique that numerically unmixes alluvial sediments to make a geochemical map of their upstream catchments. The unmixing approach uses a model that predicts the concentration of elements in downstream sediments, given a map of the drainage network and element concentrations in the source region. To unmix sedimentary chemistry, we seek the upstream geochemical map that, when mixed downstream, best fits geochemical observations downstream. To prevent overfitting we penalise the roughness of the geochemical model. To demonstrate our approach we apply it to alluvial samples gathered as part of the Northern Australia Geochemical Survey. This survey gathered samples collected over a ∼500,000 km2 area in northern Australia. We first validate our approach for this sample distribution with synthetic tests, which indicate that we can resolve geochemical variability at scales greater than 0.5–1° in size. We proceed to invert real geochemical data from the total digestion of fine-grained fraction of alluvial sediments. The resulting geochemical maps for two elements of potential economic interest, Cu and Nd, are evaluated in detail. We find that in both cases, our predicted downstream concentrations match well against a held-out, previously ‘unseen’ subset of the data, as well as against data from an independent geochemical survey. By performing principal component analysis on maps generated for all 46 available elements we produce a synthesis map to identify the significant geochemical domains in this part of northern Australia. This map shows strong spatial similarities to the underlying lithological map of the area. Finally, we compare the results from our approach to geochemical maps produced by interpolating alluvial geochemical data using two standard methods: kriging and inverse distance weighting. We find that, unlike the method presented here, both these methods can generate maps that are dampened relative to expected magnitude, as well as being spatially distorted. We argue that the unmixing approach is the most appropriate method for generating geochemical maps from regional-scale alluvial surveys.

Fractionation, Structural Characteristics, Functionality, Aromatic Profile, and In Vitro Digestibility of Lentil (Lens culinaris) Proteins

Consumers’ attitudes toward more plant-based components in their diet has greatly driven the exploration of functional plant proteins. The aim of this study was to systematically investigate the structural and functional properties, nutritional and aromatic profiles, and in vitro digestion of protein fractions from the underexploited lentil grain. Albumin (LA), globulin (LG), and its subunits legumin (LL) and vicilin (LV) were fractionated from red lentil flour through alkaline extraction–isoelectric precipitation and/or different salt extractions. LA and LV exhibited unique structural properties (more β-sheet and α-helix structures), higher solubility (almost 100% at pH 7.0), and foaming capacity (>200% at pH 7.0), while LG exhibited better emulsifying properties. LA showed a distinct aromatic profile including high numbers of volatile compounds, especially alcohols, esters, and acids. After in vitro gastric-intestinal digestion, the hydrolysis degree of LA (24.25%) was the lowest, owing to the presence of major antinutritional factors.

Acidification and alkalinization pretreatments of biowastes and their effect on P solubility and dynamics when placed in soil

BackgroundSustainability concerns as well as recent increases in fertilizer prices exacerbates the need to optimise the use of biowastes as fertilizers. For this reason, we investigated how different pretreatments affect the P dynamics when biofertilizers are placed in the soil. MethodsSewage sludge (SS), sewage sludge ash (SS-ash), meat and bone meal (MBM), and the solid fraction of biogas digestate (BGF) were pretreated with H2SO4, NaOH, and Ca(OH)2 and incubated for 2 and 12 days, respectively, in a one-dimensional reaction system for detailed studies of the interactions in the biomaterial-soil interface and the soil adjacent to the placement zone. ResultsOur results showed that acidification and treatment with NaOH increased the P solubility of the biomaterials. The P loss from the biomaterial layer to the soil was correlated with water-extractable P in the biomaterials (0.659) and water-extractable P in the soil (0.809). Acidification significantly increased the total amount of P depleted from the biomaterial to the soil whereas NaOH pre-treatment did not. However, for NaOH-treated SS and SS-ash, the apparent recoveries were significantly higher compared to the acidification due to a decrease in soil P sorption capacity as the soil pH increased due to residual alkalinity in the biomaterials. ConclusionsAcidification showed promising results by increasing the P solubility of all the biomaterials, and the alkalinization of SS and SS-ash with NaOH by increasing the apparent recovery in the soil. However, further studies are needed to assess the effects of these treatments on plant growth and P uptake.

Effect of coated cysteamine hydrochloride and probiotics supplemented alone or in combination on feed intake, nutrients digestibility, ruminal fermentation, and blood metabolites of Kamphaeng Saen beef heifers.

This study aimed to determine the effects of coated cysteamine hydrochloride (CSH) and probiotics (PB) supplemented alone or in combination on feed intake, digestibility, ruminal fermentation, and blood metabolites of heifer beef cattle. Sixteen heifers (body weight = 210 ± 41 kg; age = 9 ± 2 months) were assigned according to a randomized complete block design in a 2 × 2 factorial arrangement. All animals were fed the basal diet, which contained an 82:17 concentrate-to-forage ratio, and the forage source was rice straw. The treatments were as follows: (1) 0% PB + 0 g/d CSH, (2) 0.1% PB + 0 g/d CSH, (3) 0% PB + 20 g/d CSH, and (4) 0.1% PB + 20 g/d CSH. The main effect of CSH supplementation has been found to improve feed intake (P < 0.05). There were no treatment interactions with nutrient digestibility or rumen fermentation parameters. Supplementation of CSH did not affect any of the variables evaluated, while probiotics supplementation increased DM digestibility due to the increases in CP and fiber fraction digestibility. Compared to controls and CSH, at 16 h post-feeding, heifers receiving probiotics tended (P = 0.07) to show 17% greater ruminal NH3-N concentration, but this effect was not evident at 2 h post-feeding. However, the main effects of probiotic supplementation showed a tendency to increase the number of total bacteria and fungal zoospores in the rumen at 2 h post-feeding. The blood triglyceride (BTG) concentration of heifers fed a diet supplemented with 20 g/d CSH and 0.1% probiotics was found to be greater than those fed CSH alone (P < 0.1) at 16 h post-feeding, and then, there were greater BTG concentrations than other treatments (P < 0.05) at 2 h post-feeding. In conclusion, the combination of CSH and PB did not potentiate the effects of probiotics on digestibility and rumen fermentation and had minimal effects on blood parameters.

Digestate from Agricultural Biogas Plants as a Reservoir of Antimicrobials and Antibiotic Resistance Genes-Implications for the Environment.

Antimicrobials and antibiotic resistance genes (ARGs) in substrates processed during anaerobic digestion in agricultural biogas plants (BPs) can reach the digestate (D), which is used as fertilizer. Antimicrobials and ARGs can be transferred to agricultural land, which increases their concentrations in the environment. The concentrations of 13 antibiotics in digestate samples from biogas plants (BPs) were investigated in this study. The abundance of ARGs encoding resistance to beta-lactams, tetracyclines, sulfonamides, fluoroquinolones, macrolide-lincosamide-streptogramin antibiotics, and the integrase genes were determined in the analyzed samples. The presence of cadmium, lead, nickel, chromium, zinc, and mercury was also examined. Antimicrobials were not eliminated during anaerobic digestion. Their concentrations differed in digestates obtained from different substrates and in liquid and solid fractions (ranging from 62.8 ng/g clarithromycin in the solid fraction of sewage sludge digestate to 1555.9 ng/L doxycycline in the liquid fraction of cattle manure digestate). Digestates obtained from plant-based substrates were characterized by high concentrations of ARGs (ranging from 5.73 × 102 copies/gDcfxA to 2.98 × 109 copies/gDsul1). The samples also contained mercury (0.5 mg/kg dry mass (dm)) and zinc (830 mg/kg dm). The results confirmed that digestate is a reservoir of ARGs (5.73 × 102 to 8.89 × 1010 copies/gD) and heavy metals (HMs). In addition, high concentrations of integrase genes (105 to 107 copies/gD) in the samples indicate that mobile genetic elements may be involved in the spread of antibiotic resistance. The study suggested that the risk of soil contamination with antibiotics, HMs, and ARGs is high in farms where digestate is used as fertilizer.

Equilibrated Gas and Carbonate Standard-Derived Dual (Δ47 and Δ48) Clumped Isotope Values.

Carbonate clumped isotope geochemistry has primarily focused on mass spectrometric determination of m/z 47 CO2 for geothermometry, but theoretical calculations and recent experiments indicate paired analysis of the m/z 47 (13C18O16O) and m/z 48 (12C18O18O) isotopologues (referred to as and ) can be used to study non-equilibrium isotope fractionations and refine temperature estimates. We utilize 5,448 and 3,400 replicate measurements of carbonate samples and standards, and 183 and 195 replicate measurements of gas standards from 2015 to 2021 from a multi-year and multi-instrument data set to constrain and values for 27 samples and standards, including Devils Hole cave calcite, and study equilibrium -, -temperature, and -temperature relationships. We compare results to previously published findings and calculate equilibrium regressions based on data from multiple laboratories. We report acid digestion fractionation factors, and , and account for their dependence on the initial clumped isotope values of the mineral.

Stale bread waste recycling as ingredient for fresh oven-baked white bread: effects on dough viscoelasticity, bread molecular organization, texture, and starch digestibility.

Wasted food produced for human consumption is estimated at 33% globally, and bread is the food product with the highest percentage. There is an ongoing drive to reincorporate food waste still useful and safe into the production chain of food for human consumption. This work aims to contribute to the study of the feasibility of recycling stale bread waste flour (BWF) into fresh oven-baked white bread, by replacing 0, 20, 40, 60, 80, and 100 g/100 g of the wheat flour with BWF. Storage and loss moduli increased as the BWF content increased. The baked loaf exhibited decreased lightness and yellowness but increased redness. Increasing BWF contents produced decreased loaf volume and hardness but increased moisture content. Fourier-transform infrared analysis showed that the BWF addition induced important changes in the water, protein, and starch molecular organization. Therefore, starch digestibility showed reductions in both rapidly and slowly digestible starch fractions. Principal component analysis revealed that replacements of up 20 g/100 g can produce white bread with textural, colour, and starch digestibility characteristics like that of the control bread. The fresh oven-baked white bread variation produced by replacing 20 g/100 g of the wheat flour with BWF exhibited comparable colour, volume, texture, and starch digestibility features as a control bread did made with 0 g/100 BWF. Higher replacement percentages of wheat flour by stale BWF produced unsuitable drawbacks in the white bread characteristics, but those might be deemed as convenient in other types of bakery products. © 2023 Society of Chemical Industry.