Differential White Blood Cell Research Articles

Utility of Red Blood Cell Distribution Width to Albumin Ratio and Hemoglobin to Red Blood Cell Distribution Width Ratio as Biomarkers in Pediatric Sepsis

Objectives − The objective was to compare these parameters in children with severe and non-severe sepsis and to evaluate their utility in predicting mortality. Material and Methods − A cross-sectional study conducted in the Pediatric Intensive Care of a dis- trict hospital in India. The hematological parameters of 54 children with severe sepsis and 55 with non-severe sepsis were analysed.Results − The children with severe sepsis had significantly lower HRR (Hemoglobin-RDW ratio) with higher RDW, plateletcrit and RAR as compared to those with non-severe sepsis. There were no significant differences between the groups with respect to the differential and total white blood cell counts or their ratios and other platelet indices. As compared to the survivors, non-survivors had significantly lower HRR with higher RDW and RAR. At a cut-off value of 3.845, RAR was 79.2% sensitive and 59% specific and at a cut-off value of 0.712 HRR was 77.8% sensitive and 60% specific in prediction of mortality.Conclusions − Children with severe sepsis had higher RAR and lower HRR when compared with children with non-severe sepsis. Significantly higher RAR and lower HRR were also noted among non-survivors as compared to survivors. The biomarkers RAR and HRR are readily available and useful in predicting mortality in children with sepsis.

The association of blood eosinophil counts and FEV1 decline: a cohort study.

Accelerated lung function decline is characteristic of COPD. However, the association between blood eosinophil counts and lung function decline, accounting for current smoking status, in young individuals without prevalent lung disease is not fully understood. This is a cohort study of 629 784 Korean adults without COPD or a history of asthma at baseline who participated in health screening examinations including spirometry and differential white blood cell counts. We used a linear mixed-effects model to estimate the annual change in forced expiratory volume in 1 s (FEV1) (mL) by baseline blood eosinophil count, adjusting for covariates including smoking status. In addition, we performed a stratified analysis by baseline and time-varying smoking status. During a mean follow-up of 6.5 years (maximum 17.8 years), the annual change in FEV1 (95% CI) in participants with eosinophil counts <100, 100-199, 200-299, 300-499 and ≥500 cells·µL-1 in the fully adjusted model were -23.3 (-23.9--22.7) mL, -24.3 (-24.9--23.7) mL, -24.8 (-25.5--24.2) mL, -25.5 (-26.2--24.8) mL and -26.8 (-27.7--25.9) mL, respectively. When stratified by smoking status, participants with higher eosinophil count had a faster decline in FEV1 than those with lower eosinophil count in both never- and ever-smokers, which persisted when time-varying smoking status was used. Higher blood eosinophil counts were associated with a faster lung function decline among healthy individuals without lung disease, independent of smoking status. The findings suggest that higher blood eosinophil counts contribute to the risk of faster lung function decline, particularly among younger adults without a history of lung disease.

Antioxidant Effects of Vitamin C on Some Hematological Parameters of Male Wistar Rats Exposed to Lead Acetate

The study investigated the effects of vitamin C on platelet parameters, white blood cell count and white blood cells differentials in Wistar rats exposed to lead acetate. A total of twenty-four male Wistar rats weighing between 160g and 200g were utilized. The experimental animals were divided into four groups of six rats each (n=6). Group 1 served as the control group (received normal feed and water), group II received 10mg/kg body weight of lead acetate, group III received 100mg/kg body weight of Vitamin C, and group IV received 10mg/kg body weight of lead acetate followed by 100mg/kg body weight of Vitamin C. Lead and Vitamin C, along with normal feed, were administered for four weeks. Blood samples were collected via jugular puncture and stored in EDTA bottles for analysis to determine the blood profile of the rats. The results showed significant increase in Platelet Count (PLT) in group III and a significant decrease in Mean Platelet Volume (MPV) in group IV (Pb + vitamin C). The Mean Platelet Width (MPW) showed decrease in groups 2, 3, and 4 compared to the control group, although this decrease was not statistically significant. The study also noted an elevated level of white blood cells (WBC) in response to the antioxidant treatment, indicating a potential positive impact on immune function. In conclusion, this study demonstrates the therapeutic effect of Vitamin C against the toxic effects of lead on platelet parameters and white blood cell count and differentials.

Assessment of CD4, CD8 and White Cell Parameters Amongst HIV Seronegative Pregnant Subjects in Port Harcourt, Nigeria

Pregnancy involves maternal immunological adjustments to accommodate the fetus and maintain a strong immune defense against potential pathogens. The present study evaluated the changes in CD4, CD8, white blood cell (WBC) and total lymphocyte count (TLC) amongst HIV seronegative pregnant subjects in Port Harcourt, Nigeria. A total of 302 female subjects (18-39 years) were recruited for the study. They consisted of 205 pregnant subjects and 97 non-pregnant subjects which served as the control. All subjects were screened for HIV type 1 and type 2 using standard test kits. Total and differential white blood cell counts were determined using a haematology auto analyzer while the total lymphocyte count (TLC) was obtained by multiplying total white blood cell count (TWC) with percentage lymphocyte count. The CD4 and CD8 cell counts were analyzed using the automated flow cytometry analyzer while the CD4:CD8 cell count ratio was obtained by dividing the CD4 cell count value by that of CD8. The result of the study shows a statistically significant decrease in CD4 and CD8 cell counts, lymphocyte and total lymphocyte counts and an increase in neutrophil count in all the trimesters of pregnancy when compared to the non-pregnant control (p&amp;lt;0.05). Also, there was a significant increase in WBC during the third trimester and a similar decrease in monocyte count in the first and third trimesters of pregnancy. The evidence from the present study concludes that pregnancy modifies the maternal immune response to ensure fetal survival and the protection of the mother from invading pathogens as reported in the increase in total WBC, neutrophil and monocyte counts and a reduction in TLC, CD4 and CD8 counts. The study recommends routine assessments of these crucial cellular immune markers for pregnant women during antenatal visits.

Neutrophil-lymphocyte and Platelet-lymphocyte Ratios of Pregnant Women Living with HIV/AIDS

Background: Chronic inflammation, a factor for high mortality, persists throughout the pregnancy period of women living with human immunodeficiency Virus (HIV).&#x0D; Aim: To determine whether pregnancy alone or the combined effects of HIV and pregnancy are the sources of the inflammation, this study evaluated the predictive variables for inflammation, lymphocyte ratio (NLR), and Platelet-Lymphocyte Ratio (PLR).&#x0D; Materials and Methods: A population of 128 women, aged 15-49 years were grouped into 4; Group A- 32 HIV positive and pregnant, Group B- 32 HIV negative and pregnant, Group C- 32 HIV positive not pregnant, Group D- 32 HIV negative not pregnant (control group). Differential white blood cell and platelet count methods were used to determine the neutrophil (N), Lymphocyte (L) and platelet (P) counts. The ratio calculation method was used to derive the NLR and PLR.&#x0D; Results: NLR was significantly higher for group A (2.62±0.41) compared to groups B (2.25 ± 0.18); C (0.95 ± 0.07); D (1.68 ± 0.19) at p ≤ 0.00. However, the NLR of groups A and B (p ≤ 0.05) were not significantly different, PLR was significantly higher for group A (9.86±1.12) compared to groups B (6.65 ± 1.12); C (5.35 ± 0.47); D (6.34 ± 0.43). There was no significant difference in blood pressure across the groups, significant difference was however observed in their Body Mass Index.&#x0D; Conclusion: Hence, Inflammation may have been caused by pregnancy alone.

Comparison of the Haematological Parameters of Indigenous and Exotic ‎Dogs in Maiduguri, Nigeria

Health status of dogs may be assessed with haematological parameters, but adaptation to environmental stress can lead to adjustment of these normal parameters. This study was designed to compare the haematological parameters of indigenous and exotic dogs in Maiduguri during the hot dry season. Forty apparently healthy indigenous (n = 20) and exotic(n = 20) dogs of both sexes and aged from 3-48 months were used. Blood sample (5 ml) was collected from the cephalic vein of each dog in EDTA bottles. Parameters were determined using microhaematocrit method for packed cell volume (PCV), haemocytometery for red blood cell (RBC) count and total white blood cell (TWBC) count, cyanmethemoglobin method for haemoglobin concentration (Hb), and examination of stained blood smear for relative differential white blood cell (WBC) count. Mean corpuscular volume (MCV) and haemoglobin concentration (MCHC), and absolute differential WBC counts were calculated. The TWBC and neutrophil counts were significantly (p &lt; 0.05) higher in indigenousthan exotic dogs. Exotic females had significantly (p &lt; 0.05) lower MCHC and neutrophil count than indigenous females and all males. Age significantly (p &lt; 0.05) affected MCV, but the highest value was recorded in young exotic dogs. Adult indigenous dogs had significantly (p &lt; 0.05) higher neutrophil count than young indigenous and all exotic dogs. Monocyte count was significantly (p &lt; 0.05) higher in young than adult exotic and all indigenous dogs. However, haematologicalvalueswere within standard reference intervals for dogs.

Effect of Dexamethasone in Feed on Intestinal Permeability, Differential White Blood Cell and Immune Organs in Rabbits

Effect of Dexamethasone in Feed on Intestinal Permeability, Differential White Blood Cell and Immune Organs in Rabbits

Performance Assessment of Sysmex DI-60: Is Digital Morphology Analyzer Reliable for White Blood Cell Differentials in Body Fluids?

Few studies have evaluated digital morphology (DM) analyzers on body fluids (BF). We evaluated the performance of a DM analyzer, Sysmex DI-60 (Sysmex, Kobe, Japan) for white blood cell (WBC) differentials in BF samples. In five BF samples (two pleural fluids and three ascites) containing a single, dominant cell type (>80%, neutrophils, lymphocytes, macrophages, abnormal lymphocytes, and malignant cells in each sample), we evaluated the precision of the DI-60 and compared the WBC differentials and turnaround times (TAT) between DI-60 and manual counting. The precision of the DI-60 pre-classification and verification was excellent (%CV, 0.01-3.16%). After verification, the DI-60 showed high sensitivity, specificity, and efficiency (ranges: 90.8-98.1%, 96.8-97.9%, and 92.5-98.0%, respectively) for the dominant cell types in neutrophil- and lymphocyte-dominant samples. For all samples, the DI-60 and manual counting showed high correlations for major cell types (neutrophils, lymphocytes, macrophages, and others, r = 0.72 to 0.94) after verification. The agreement between the pre-classification and verification of the DI-60 was strong in the neutrophil-dominant sample (κ = 0.81). The DI-60 showed a significantly longer TAT (min: s) than manual counting for all samples (median TAT/slide: 6:28 vs. 1:53, p < 0.0001), with remarkable differences in abnormal lymphocyte- and malignant cell-dominant samples (21:05 vs. 2:06; 12:34 vs. 2:25). The DI-60 may provide reliable data in neutrophil- and lymphocyte-dominant BF samples. However, it may require longer times and higher workloads for WBC differentials especially in BF samples containing atypical cells. Further improvement would be needed before applying DM analyzers for routine clinical practice in BF analysis.

Paradoxical eosinophilic and cytokine responses to oral corticosteroid treatment in patients with asthma exacerbations

BackgroundThymic stromal lymphopoietin (TSLP) orchestrates eosinophilic inflammation, which may increase in asthma exacerbations. On the contrary, miR-1 inhibits TSLP-mediated eosinophil trafficking in lung endothelium. Whether the balance of TSLP and miR-1 levels determines the response to oral corticosteroids during the treatment of asthma exacerbations remains unknown. ObjectiveTo investigate the involvement of TSLP/miR-1 axis in inflammatory response to OCS treatment for asthma exacerbations. MethodsWe measured the concentrations of TSLP and other inflammatory cytokines and miR-1 expression during acute asthmatic exacerbations treated with standard oral corticosteroids (OCS) in a real-life setting. Twenty eight consecutive patients with acute asthma exacerbations treated with OCS for 1 week at the Emergency Department (ED) were studied prospectively. Steroid responders (SR) were identified by a significant reduction in blood eosinophils, whereas paradoxical responders (PR) showed no markedly decreased or even increased absolute blood eosinophil counts after OCS treatment. Differential white blood cell counts, blood cytokine levels and miR-1 expression within and between groups were compared before and after OCS treatment. The baseline cytokine concentrations in both groups were compared with those of stable asthmatics (SA). ResultsOCS treatment significantly reduced TSLP levels in steroid responders whereas this effect did not occur in paradoxical responders (P = 0.006 and P = 0.742, respectively). In contrast, miR-1 expression was unchanged in steroid responders in response to OCS, whereas it was markedly reduced in the paradoxical responders, despite higher expression at baseline compared with stable asthmatics, which may account for slower resolution of the exacerbation. ConclusionsIn some asthmatic patients with acute exacerbations who do not suppress eosinophils after a course of OCS, there is a paradoxical decrease in plasma miR-1 and increase in TSLP compared with steroid responders, which may result in slower clinical recovery.

The Role of Rosmarinic Acid in the Protection Against Inflammatory Factors in Rats Model With Monocrotaline-Induced Pulmonary Hypertension: Investigating the Signaling Pathway of NFκB, OPG, Runx2, and P-Selectin in Heart.

Shortness of breath and syncope are common symptoms of right ventricular failure caused by pulmonary arterial hypertension (PAH), which is the result of blockage and increased pressure in the pulmonary arteries. There is a significant amount of evidence supporting the idea that inflammation and vascular calcification (VC) are important factors in PAH pathogenesis. Therefore, we aimed to investigate the features of the inflammatory process and gene expression involved in VC in monocrotaline (MCT)-induced PAH rats. MCT (60 mg/kg, i.p.) was used to induce PAH. Animals were given normal saline or rosmarinic acid (RA) (10, 15, and 30 mg/kg, gavage) for 21 days. An increase in right ventricular systolic pressure was evaluated as confirming PAH. To determine the level of inflammation in lung tissue, pulmonary edema and the total and differential white blood cell counts in the bronchoalveolar lavage fluid were measured. Also, the expression of NFκB, OPG, Runx2, and P-selectin genes was investigated to evaluate the level of VC in the heart. Our experiment showed that RA significantly decreased right ventricular hypertrophy, inflammatory factors, NFκB, Runx2, and P-selectin gene expression, pulmonary edema, total and differential white blood cell count, and increased OPG gene expression. Therefore, our research showed that RA protects against MCT-induced PAH by reducing inflammation and VC in rats.

Rosmarinic acid improves tracheal smooth muscle responsiveness and lung pathological changes in ovalbumin-sensitized rats

Objective: To evaluate the effect of rosmarinic acid on tracheal smooth muscle responsiveness and lung pathological changes in ovalbumin-sensitized rats. Methods: Rats were randomly divided into six groups: the control group, the asthmatic group, and the asthmatic groups treated with dexamethasone (1 mg/kg; oral gavage) or three doses of rosmarinic acid (0.5, 1, and 2 mg/kg; oral gavage). For induction of asthma, rats received intraperitoneal injections and inhalation of ovalbumin. After 21 days, bronchoalveolar lavage fluid and lung samples were collected for histopathological analyses. Moreover, total and differential white blood cell counts were determined. Results: The rosmarinic acid-treated group had significantly lower tracheal smooth muscle responses to methacholine than the asthmatic group. In addition, rosmarinic acid reduced white blood cell count and the percentages of eosinophils, monocytes, and neutrophils while increasing the percentage of lymphocytes. Ovalbumin-induced lung pathological changes were significantly improved by treatment with rosmarinic acid. Conclusions: Rosmarinic acid improves tracheal smooth muscle responsiveness and lung pathological changes in ovalbumin-sensitized rats.

Evaluation of coriander seed powder supplementation on body composition, growth performance, hematology, and serum biochemical indices in common carp (Cyprinus carpio) fingerlings.

This study aimed to investigate the role of coriander seed powder (Coriandrum sativum) on growth indices, feed utilization, body composition, and haemato-biochemical parameters in common carp (Cyprinus carpio) fingerlings over 84 days. One hundred and forty-four common carp (25.24±0.05 g) were assigned into four groups fed with different diets, namely 0 (basal diet), 1%, 2%, and 4% of coriander seed powder (CSP). In the current study, C. carpio fed with dietary CSP revealed significant improvement in weight gain, final weight, specific growth rate, total feed intake, feed conversion efficiency, feed conversion ratio, protein intake, and protein efficiency ratio, in comparison to control fish fed after 84 days (P>0.05). It was also found that fish fed with 1%CSP-supplemented dietary had the best growth performance and feed utilization. The crude protein of fish fed with CSP dietary treatments increased, and significant differences were only found in the fish fed with 1%CSP diet, in comparison to the control group. The CSP supplementation groups showed significant increases in hemoglobin, hematocrit, albumin, total protein, and globulin compared to the control group. Nevertheless, differential white blood cells, mean corpuscular hemoglobin concentration, cholesterols, and triglycerides were significantly reduced in the CSP dietary group in comparison to the control group. It was also found that CSP dietary treatment significantly increased lipase and amylase in comparison to the control group (P>0.05). However, the highest lipase and amylase levels were obtained at 1%CSP and 2%CSP dietary treatment groups, compared to the control basal diet. Based on the results, CSP supplementation could improve the overall health status and growth performance of common carp fingerlings.

Alterations in haematological indices of indomethacin-ulcerated rats treated with Persea americana seed and Bryophyllum pinnatum leaf ethyl acetate fraction

This study determined the alterations in haematological parameters of indomethacin-ulcerated rats treated with P. americana seed (PAS) and B. pinnatum leaf (BPL) ethyl acetate fraction. Fifty (50) healthy Wistar albino rats were randomly assigned into ten (10) groups of five (5) animals each according to body weight (100-120 g). Animals in group I served as normal control; the rats in groups III-X were pre-treated with 20 mgKg-1 b. wt. Omeprazole (STD), 400 mgKg-1 b. wt. PAS, 400 mgKg-1 b. wt. BPL, 400 mgKg-1 b. wt. PAS + BPL (1:1), 400 mgKg-1 b. wt. PAS + BPL (1:2), 400 mgKg-1 b. wt. PAS + BPL (1:3) respectively for 21 days. Thereafter, animals in groups II-X were induced for gastric ulcer by intubation of 30 mgKg-1 b. wt. indomethacin after being fasted for 24 hours. The animals were sacrificed after 4 hours and the complete blood count was determined using an automated hematology analyzer. Results obtained from the study showed significant (p&lt;0.05) elevation in the total and differential white blood cell count of Wistar rats in the indomethacin-induced gastric ulcer control group and ethyl acetate fraction treated groups. Indomethacin, PAS, and BPL ethyl acetate fraction and the combinations did not cause marked changes in red blood cell indices. However, platelet count was significantly (p&lt;0.05) reduced by the combinations of the P. americana seed and B. pinnatum leaf ethyl acetate fraction. These findings demonstrated that indomethacin induction resulted in a significant increase in the total white blood cell and platelet count when matched with the control group; this was similarly seen in PAS+BPL (1:3) and PAS+BPL (3:1) combinations of PAS and BPL. The result is suggestive of a compromise in the hemostatic capability of the blood in rats treated with some combinations of P. americana seed and B. pinnatum leaf ethyl acetate fraction.

A comparison study between the Siemens ADVIA 120 and manual method for the differential white blood cell count in goats.

Although widely used, the ADVIA 120 hematology analyzer has not been previously validated for determining the differential leukocyte count in goats. The aim of this study was to compare the differential leukocyte counts provided by the ADVIA 120 (A-diff) and the manual method (M-Diff) in goats. EDTA blood samples that were analyzed within 4 h of collection were used in the study. The following exclusion criteria were applied: inappropriately filled tubes or tubes containing clots, erroneous ADVIA peroxidase cytograms, and blood smears of poor quality. The A-Diff was compared with the M-Diff performed by two independent observers on 200 leukocytes. Forty samples were included after previously excluding eight samples. The correlation between the A-Diff and M-Diff was very strong for eosinophils (r = .870, p < .001) and strong for lymphocytes (r = .796, p < .001) and neutrophils (r = .730, p < .001), while no significant correlation was observed for monocytes (r = .026, p = .872). The Passing-Bablok regression analyses revealed statistically significant constant errors for neutrophils (5.83%; 95% confidence interval [CI]: 0.41%, 12.18%) and eosinophils (1.89%; 95% CI: 1.17%, 2.71%). Bland-Altman analyses showed a statistically significant negative bias for lymphocytes (-5.0%) and a statistically significant positive bias for eosinophils (2.2%). The very low basophil percentages precluded a meaningful method comparison. The ADVIA 120 overall demonstrated good performance for the differential WBC count in goats under the conditions of this study. Therefore, it can be considered suitable for routine hematologic screening in goats. Nonetheless, it should be emphasized that any abnormal result should be confirmed with a blood smear evaluation.

Cognition and its relationship with differential white cell count among obese undergraduates in Nigeria

Abstract Introduction: Studies have linked obesity with cognitive impairment and low-grade inflammation; however, results have been inconsistent. The aim of this study was to compare global cognitive function and its relationship with indices of obesity and total and differential white blood cell (WBC) counts in obese and normal-weight undergraduates in Kano, Nigeria. Materials and Methods: Eighty participants (40 obese and 40 normal weights) were randomly recruited for this case–control descriptive study. Anthropometric indices were measured according to the World Health Organization’s Stepwise protocol; global cognitive function was assessed using the Montreal Cognitive Assessment (MoCA), while the WBC count was performed by manual Leishman staining technique. Data were analyzed on statistical package for social science using an independent t-test, Chi-square test of association, Pearson’s correlation, and simple linear regression. Results: The mean age of the participants is 24 years, obese a year older (t = -2.2, P = 0.030). There was no significant difference in global cognitive function score (MoCA score) between the obese and normal-weight participants (t = -1.08, P = 0.283). However, 40% of the normal weight and 42% of the obese participants had mild cognitive impairment. Similarly, the two groups did not differ in total and differential WBC count except for %monocyte, which was higher in normal-weight participants (t = 2.22, P = 0.029). None of the anthropometric and WBC indices correlated with the MoCA score; however, %monocyte was negatively correlated with body mass index (BMI) (r = -0.244, P = 0.029), waist circumference (r = -0.216, P = 0.050), hip circumference (r = -0.234, P = 0.037), and wait-to-height ratio (r = -0.245, P = 0.028) among the whole while %lymphocyte was negatively correlated with BMI (r = -0.311, P = 0.050) among obese participants. Conclusion: Obesity is not associated with a decline in cognitive function and inflammation in obese and normal-weight undergraduates in Nigeria.

Systemic and Lung Inflammation and Oxidative Stress Associated With Behavioral Changes Induced by Inhaled Paraquat Are Ameliorated by Carvacrol.

Paraquat (PQ) is an herbicide toxin that induces injury in different organs. The anti-inflammatory and antioxidant effects of carvacrol were reported previously. The effects of carvacrol and pioglitazone (Pio) alone and their combination on inhaled PQ-induced systemic and lung oxidative stress and inflammation as well as behavioral changes were examined in rats. In this study, animals were exposed to saline (control [Ctrl]) or PQ (PQ groups) aerosols. PQ-exposed animals were treated with 0.03 mg/kg/day dexamethasone (Dexa), 20 and 80 mg/kg/day carvacrol (C-L and C-H), 5 mg/kg/day Pio, and Pio+C-L for 16 days. Inhaled PQ markedly enhanced total and differential white blood cell (WBC) counts, nitric oxide (NO), and malondialdehyde (MDA) levels but decreased catalase (CAT) and superoxide dismutase (SOD) activities and thiol levels both in the bronchoalveolar lavage fluid (BALF) and blood and increased interferon-gamma (INF-γ) and interleukin-10 (IL-10) levels in the BALF (p < 0.001 for all cases) except lymphocyte count in blood which was not significantly changed. The escape latency and traveled distance were increased in the PQ group. However, the time spent in the target quadrant in the Morris water maze (MWM) test and the duration of time latency in the dark room in the shuttle box test were reduced after receiving an electrical shock (p < 0.05-p < 0.001). Inhaled PQ-induced changes were significantly improved in carvacrol, Pio, Dexa, and especially in the combination of the Pio+C-L treated groups (p < 0.05-p < 0.001). Carvacrol and Pio improved PQ-induced changes similar to Dexa, but ameliorative effects produced by combination treatments of Pio+C-L were more prominent than Pio and C-L alone, suggesting a potentiating effect for the combination of the two agents.

Some Hematological Indices as Predictors of Survival in Chronic Myeloid Leukemia Patients

Abstract Background: Despite the promising of introduction of tyrosine kinase inhibitors (TKIs), chronic myeloid leukemia (CML) remains a significant cause of annual mortality. Red blood cell distribution width (RDW), neutrophil/lymphocyte ratio (NLR), and platelet/lymphocyte ratio (PLR) are parameters derived from a complete blood count (CBC) commonly used to diagnose anemia, autoimmune diseases, and inflammation. These parameters have been reported to have a strong association with various diseases, including hematologic malignancies. Objectives: The study aims to identify whether RDW, NLR, and PLR can act as predictors of survival in newly diagnosed and treated CML patients. Materials and Methods: The study involved 60 Iraqi patients (37 males, 23 females, aged 17–69 years) with CML at chronic phase, who were referred to the National Center of Hematology/Mustansiriyah University, Baghdad, from February 2022 to December 2022. Twenty were newly diagnosed (T0), and 40 were under TKI treatment (T+), with 20 on imatinib and 20 on nilotinib. Additionally, a control group of 20 age- and gender-matched healthy subjects was included. CBC assessed red blood cell (RBC) indices across all groups. Results: There was no significant difference in the age of CML patients at the onset of disease between males (34.5 ± 11.7 years) and females (34 ± 11.9 years). Likewise, there was no significant difference in the treatment of CML patients with imatinib or nilotinib between males (48% and 52%) and females (53.3% and 47.7%), respectively. Most RBC indices for patients and controls were within normal ranges without significant differences. However, RDW% in T0 was markedly elevated (20.4%), with about 80% showing anisocytosis, surpassing both T+ and controls, and exceeding the upper limit of normal. The total and differential white blood cell (WBC) counts were significantly higher in T0 compared to T+, exceeding their normal ranges. Additionally, the NLR was significantly higher in T0 (8.13) compared with T+ and controls (1.80 and 1.87, respectively). Platelet count, mean platelet volume, and platelet distribution width (PDW%) differed significantly among the three groups but remained within the normal range. However, PLR in T0 (31 ± 24) was significantly lower than those in T+ and controls (130 ± 43 and 102 ± 27, respectively). Conclusion: It can be concluded that the monitoring of some parameters in peripheral blood in CBC test (as a simple and inexpensive test) such as RDW%, NLR%, and PLR% during the therapy course of CML patients may act as predictive markers to evaluate the prognosis of disease in CML patients and the degree of response to certain TKI treatment.

Conformity of Differential White Blood Cell (WBC) Examination Results on the Sysmex DI-60 And Sysmex XN-1000 at Prof. Dr. IGNG Ngoerah General Hospital, Denpasar, Bali

Conformity of Differential White Blood Cell (WBC) Examination Results on the Sysmex DI-60 And Sysmex XN-1000 at Prof. Dr. IGNG Ngoerah General Hospital, Denpasar, Bali

Cellular Inflammatory Markers and the Castelli Risk Indices in Women with Gestational Diabetes Mellitus

Background: The clinical importance of early identification of potential predictors of cardiovascular events in women with gestational diabetes mellitus (GDM) cannot be overemphasized. Thus, this study was aimed to determine the levels of selected cellular inflammatory markers and Castelli I and II risk indices in women with GDM. Methods: A total of 40 pregnant women, consisting of 11 women with GDM and 29 women without GDM, were randomly enrolled in this case-control study using the convenient sampling method. After participants fasted overnight, venous blood samples were taken, and the plasma lipid profiles were determined using the spectrophotometric methods. Then, differential white blood cells counting was done using a microscope. Thereafter, plasma low-density lipoprotein cholesterol (LDL-C) levels, Castelli risk index-I (CRI-I) and CRI-II, neutrophil-lymphocyte ratio (NLR), and monocyte lymphocyte ratio (MLR) were calculated using the appropriate formula. Student’s t-test, Mann Whitney U, Chi-square, and Spearman’s rho correlations were used for statistical analysis. P-values less than 0.05 were considered statistically significant.Results: Mean age, gestational weight, fasting plasma glucose (FPG), total cholesterol (TC), triglyceride (TG), LDL-C, and neutrophil count were significantly higher in women with GDM. Likewise with the Castelli I and II risk indices values, as well as the NLR. In contrast, HDL-C levels, lymphocytes, and monocytes counts were significantly lower in women with GDM. However, the two groups observed no difference in the MLR ) .Conclusion: Gestational diabetes mellitus is associated with dyslipidemia and systemic inflammation, which are forerunners of cardiovascular diseases.

Sex differences in plasma metabolites in a guinea pig model of allergic asthma.

Sex seems to be a contributing factor in the pathogenesis of bronchial asthma. This study aimed to find sex-related differences in metabolome measured by hydrogen-1 nuclear magnetic resonance ((1)H NMR) spectroscopy in healthy and ovalbumin (OVA)-sensitized guinea pigs. Adult male and female animals were divided into controls and OVA-sensitized groups. OVA-sensitization was performed by OVA systemic and inhalational administration within 14 days; on day 15, animals were killed by anesthetic overdose followed by exsanguination. Blood was taken and differential white blood cell count was measured. Left lung was saline-lavaged and differential cell count in the bronchoalveolar lavage fluid (BALF) was measured. After blood centrifugation, plasma was processed for (1)H NMR analysis. Metabolomic data was evaluated by principal component analysis (PCA). Eosinophil counts elevated in the BALF confirming eosinophil-mediated inflammation in OVA-sensitized animals of both sexes. Sex differences for lactate, glucose, and citrate were found in controls, where these parameters were lower in males than in females. In OVA-sensitized males higher glucose and lower pyruvate were found compared to controls. OVA-sensitized females showed lower lactate, glucose, alanine, 3-hydroxy-butyrate, creatine, pyruvate, and succinate concentrations compared to controls. In OVA-sensitized animals, lactate concentration was lower in males. Data from females (healthy and OVA-sensitized) were generally more heterogeneous. Significant sex differences in plasma concentrations of metabolites were found in both healthy and OVA-sensitized animals suggesting that sex may influence the metabolism and may thereby contribute to different clinical picture of asthma in males and females.