The present work aims to evaluate the antiinflammatory and antioxidant activities of the Maytenus senegalensis (Lam.) roots hydroethanolic extract and to determine its active fraction. The total extract is fractioned in cold alcohol 75%. Phytochemical studies and in vitro antioxidant test are performed on the extract and obtained fractions. During 28 days, mice were sensitized and challenged with OVA to induce allergic airway inflammation. the anti-inflammatory effect of the plant extract and its fractions is assessed by differential white blood cell counts and histopathological examinations. Them antioxidant effect is evaluated in vivo by MDA and glutathione dosage. Qualitative and quantitative phytochemical tests showed that the extract and its fractions contain phenolics compounds. In vitro, both extract and its fractions expressed antioxidant activities. Treatment with M. senegalensis extract and its fractions significantly decreased inflammatory cells number in BALF, reduced MDA concentration and enhanced glutathione level (p<0.05; p<0.01; p<0.001) in lung homogenate. Histological observations confirmed the anti-inflammatory effect of the extract and its fractions. Comparatively, the supernatant fraction showed the best effect. This study may land up in establishing the scientific evidences for the traditional claims anti-asthmatic and anti-inflammatory activity of Maytenus senegalensis (Lam.). It also suggests that the phytochemical groups involved in this activity would be more concentrated in the supernatant fraction.