The anti HIV drug Perfenazine has been qualitatively and quantitatively analyzed by direct current polarographic (DCP), differential pulse polarographic(DPP) and amperometric technique. Physicochemical, and microbial studies on Fe (III)-Perfenazine complex have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies and amperometric titrations, the metal: drug ratio has been worked out to be 1: 1. The metal ligand interaction has been studied using polarographic method at 25±1°C and at ionic strength of μ = 1.0 (KCI). IR spectral studies have been used to ascertain metal:ligand binding site which speaks of the complex formation between the metal and the Perfenazine ligand through the primary -0H group. Microbial studies on the complex was done against various pathogenic bacteria viz. Escherichia coli, Salmonella typhi, Vibrio cholerae and Diplococcus pneumoniae. The results of the microbial studies with the metal: drug complex revealed that the drug: metal complex was more potent against the pathogenic bacteria as compared to the pure drug, Perfenazine. As such Fe (III)-Perfenazine complex may be recommended to the therapeutic experts for its possible use as more potent anti-Aids drugs.