The objective of this study was to determine the biochemical and microbiological attributes in areas with different vegetation covers (mature forest, secondary forest, and sugar cane monoculture) and seasonal changes, and to compare the sensitivity of these attributes types (mycorrhizal, enzymatic, and carbon attributes) in response to changes in vegetation cover and season. Soil samplings were conducted over two years (one per semester, during the dry and wet periods of 2007 and 2008) in three types of areas: (I) mature forest; (II) secondary forest; and (III) sugar cane crop. The enzymatic activity of fluorescein diacetate, dehydrogenase, saccharase, basal carbon respiration, the carbon content of microbial biomass, the microbial coefficient, total and easily extracted glomalin soil-related proteins, mycorrhizal colonization, the number of glomerospores, total organic carbon, fulvic and humic acid content, and the fulvic/humic acid ratio were analyzed. In general, mycorrhizal parameters were more sensitive in distinguishing areas with different types of vegetation cover. Additionally, carbon stocks in areas of mature and secondary forest were higher compared to areas cultivated with sugar cane. The study reveals that mycorrhizal properties, such as root colonization and GRSP fractions, are more sensitive indicators of soil health than soil C attributes, providing a cost-effective alternative for evaluating soil health in the Atlantic Forest. The confirmed hypothesis supports the effectiveness of microbiological activity and C dynamics in discerning significant changes between sugar cane cultivation and forested areas. The study underscores the vital role of conserving forested areas, serving as crucial carbon reservoirs, and emphasizes the potential for enhanced sustainability through conservation practices and rapid soil function recovery in secondary forests within the Atlantic Forest.
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