Different Stages Of Maturation Research Articles

Physicochemical characterisation of a new Myrtaceae fruit native to Brazilian Cerrado

ABSTRACT A new fruit of the Myrtaceae, Eugenia bonita, was recently discovered. Its composition is unknown, which makes its physicochemical characterisation and its development profile during maturation necessary. Therefore, this work constitutes the first study aimed at determining the quality parameters and bioactive compounds of new fruit of E. bonita, at different stages of maturation. The fruits were collected in the city of Bonito de Minas, Minas Gerais in five stages of maturation and submitted for analysis of the physicochemical characteristics. The advance of maturation caused increase in all biometric characteristics of E. bonita fruits and reduction in all constituents of the proximate composition, except moisture. In addition, the ripe fruit was characterised by high water content, high acidity, and good sweetness. Moreover, there was change in the fruit color from green to yellow-orange, after maturity. In turn, although some phytochemicals have degraded during maturation, the high levels of vitamin C (61.17 mg/100 g) and total phenolics (587.92 mg GAE/100 g) in the ripe fruits characterise E. bonita as an important source of bioactive compounds. These data are of great importance to the scientific community, food industry, and popular culture since this fruit has interesting functional properties and great nutritional value.

Targeting glucose metabolism with dichloroacetate (DCA) reduces zika virus replication in brain cortical progenitors at different stages of maturation

The underlying threat of new Zika virus (ZIKV) outbreaks remains, as no vaccines or therapies have yet been developed. In vitro research has shown that glycolysis is a key factor to enable sustained ZIKV replication in neuroprogenitors. However, neither in vivo nor clinical investigation of glycolytic modulators as potential therapeutics for ZIKV-related fetal abnormalities has been conducted. Accordingly, we tested the therapeutic potential of metabolic modulators in relevant in vitro systems comprising two pools of neuroprogenitors (NPCs), which resemble early and late stages of pregnancy. Effective doses of metabolic modulators [3.0 μM] dimethyl fumarate (DMF), [3.2 mM] dichloroacetate (DCA), and [6.3 μM] VER-246608 were determined for these cells by their effect on lactate release, pyruvate dehydrogenase (PDH) activity and cell survival. The drugs were used in a 24h pre-treatment and kept throughout ZIKV infection of NPCs. Drug effects and ZIKV replication were assessed at 24- and 56-h post-infection. In early NPCs treated with DMF, DCA and VER-246608, there was a significant reduction in the extracellular release of ZIKV potentially by PDH-mediated increased mitochondrial oxidation of glucose. Out of the three drugs, only DCA was observed to reduce viral replication in late NPCs treated with DCA. Altogether, our findings suggest that reduction of anaerobic glycolysis could be of therapeutic potential against ZIKV-related fetal abnormalities and that clinical translation should consider the use of specific glycolytic modulators over different trimesters.

The Reproductive Lifespan of Ovarian Follicle.

The functional unit within mammalian ovaries is the ovarian follicle. The development of the ovarian follicle is a lengthy process beginning from the time of embryogenesis, passing through multiple different stages of maturation. The purpose of this review is to describe the most basic events in the journey of ovarian follicle development, discussing the importance of ovarian reserve and highlighting the role of several factors that affect oocyte quality and quantity during aging including hormonal, genetic and epigenetic factors. Novel, promising anti-aging strategies are also discussed.

Cladodes of Opuntia ficus-indica (L.) Mill. Possess Important Beneficial Properties Dependent on Their Different Stages of Maturity.

Opuntia ficus-indica (L.) Mill. belongs to the Cactaceae family and the genus Opuntia; it is a succulent plant that adapts to extreme climatic conditions. The aerial part of the plant consists of the cladodes, morphological changes of branches that appear green, are covered with thorns, and are essential to reduce excessive perspiration of water. The composition of cladodes is very varied, and the main constituents are water, fibers, polysaccharides, proteins, fatty acids, vitamins, sterols, minerals, and polyphenols. Polyphenols are responsible for many beneficial activities for human health, such as antioxidant, anti-inflammatory, anticancer, and nutritional properties. The purpose of this manuscript was to compare the properties of cladodes belonging to the same plant but with different stages of maturity. Relative extracts were tested both in vitro and on a cell line and antioxidant and anti-apoptotic properties were found. The antioxidant activity was tested by the Oxygen Radical Absorbance Capacity (ORAC) test, the 1,1-diphenyl-2-picrylhydrazil (DPPH) test, and the measurement of cellular accumulation of reactive oxygen species (ROS). Anti-apoptotic activity was evaluated by the annexin/PI assay and measurement of caspases 9 and 3 expression. The results obtained showed that the extracts considered possess antioxidant and anti-apoptotic properties. However, the different stages of maturity of cladodes are essential for the performance of both functions. In addition, important variations were made in the dissolution of the extracts that brought greater safety in their use. In conclusion, this manuscript provides further information on cladodes of Opuntia ficus-indica, which can be used as adjuvants in many human pathologies.

Development of Thai-taste maoberry (Antidesma bunius (L.) Spreng.) vegan sorbet from fruit of different maturities with added inulin: Physicochemical and antioxidant properties

Maoberry (Antidesma bunius (L.) Spreng.) is a tropical fruit popularly consumed with Thai-style chili sugar flakes containing sugar, salt, and chili to enhance its flavour, and reduce its sour and bitter taste. The present work aimed to develop a Thai-taste maoberry vegan sorbet using maoberries at different stages of maturity with added inulin, and to evaluate its physicochemical properties, total phenolic and total flavonoid contents, and antioxidant capacities. Results showed that the three different stages of maoberry maturity caused significant changes on pH, total soluble solids, mixture viscosity, overrun, and firmness of the sorbets. The antioxidant activities assessed using ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 3-ethylbenzthiazoline-6-sulphonic acid (ABTS), radical cation decolourisation, and oxygen radical absorbance capacity (ORAC) assays indicated similar trends. Black maoberry sorbet exhibited total flavonoid contents and FRAP values almost twice those of red maoberry sorbet. However, adding inulin significantly increased the melting rate of the product (p < 0.05), but improved the mixture viscosity and overrun, which are important attributes of sorbets.

The types and numbers of kinesins and dyneins transporting endocytic cargoes modulate their motility and response to tau

Organelles and vesicular cargoes are transported by teams of kinesin and dynein motors along microtubules. We isolated endocytic organelles from cells at different stages of maturation and reconstituted their motility along microtubules in vitro. We asked how the sets of motors transporting a cargo determine its motility and response to the microtubule-associated protein tau. Here, we find that phagosomes move in both directions along microtubules, but the directional bias changes during maturation. Early phagosomes exhibit retrograde-biased transport while late phagosomes are directionally unbiased. Correspondingly, early and late phagosomes are bound by different numbers and combinations of kinesins -1, -2, -3, and dynein. Tau stabilizes microtubules and directs transport within neurons. While single-molecule studies show that tau differentially regulates the motility of kinesins and dynein in vitro, less is known about its role in modulating the trafficking of endogenous cargoes transported by their native teams of motors. Previous studies showed that tau preferentially inhibits kinesin motors, which biases late phagosome transport towards the microtubule minus-end. Here, we show that tau strongly inhibits long-range, dynein-mediated motility of early phagosomes. Tau reduces forces generated by teams of dynein motors on early phagosomes and accelerates dynein unbinding under load. Thus, cargoes differentially respond to tau, where dynein-complexes on early phagosomes are more sensitive to tau inhibition than those on late phagosomes. Mathematical modeling further explains how small changes in the number of kinesins and dynein on cargoes impact the net directionality but also that cargoes with different sets of motors respond differently to tau.

Status of the reproductive system of anchovy sprat (Clupeonella engrauliformis, Borodin, 1904) in the Caspian sea

The impact of negative factors on the population of anchovy sprat (Clupeonella engrauliformis, Borodin, 1904) continues, and therefore in recent years there has been a steady downward trend in the abundance and density of the species. Research catches of anchovy sprat are declining. One of the conditions contributing to the population recovery is an optimal, without critical pathologies condition of reproductive system of individuals. The purpose of this work is to study the condition of testes and ovaries of anchovy sprat. According to the histological picture of germ cells it is possible to objectively assess the state of reproductive ability of individuals. The study was carried out using the methods of classical histology. The material was gonads of females and males sampled in the summer of 2023 in the Middle Caspian Sea. According to the results of histological study it was found that gonads have different stages of maturity (II–III, III, IV and V). The presence of direct correlations between maturity indices (gonad weight, gonadosomatic index, maturity coefficient) in individuals of both sexes was found. In females, the size of oocytes of different stages of maturity is described, and the absence of correlation between cell diameter and length and mass of the individual is revealed. Distinctive features of resorption of oocytes of III and IV stages of maturity by such characteristics as shape, color and transparency of the cell are given. The proportion of females with resorbed oocytes (67%) and the average proportion of cells with developmental abnormalities (24%) were noted. The results of this study may contribute to a better understanding of the processes occurring in the reproductive glands of male and female anchovy sprat, which is very important for fish reproduction.

Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity.

The irresponsible overuse of antibiotics has increased the occurrence of resistant bacterial strains, which represents one of the biggest patient safety risks today. Due to antibiotic resistance and biofilm formation in bacteria, it is becoming increasingly difficult to suppress the bacterial strains responsible for various chronic infections. Honey was proven to inhibit bacterial growth and biofilm development, offering an alternative solution in the treatment of resistant infections and chronic wounds. Our studies included chestnut honey, valued for its high antibacterial activity, and the bacteria Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and S. epidermidis, known to form multi-species biofilm communities. Minimum inhibitory concentrations (MIC) of chestnut honey were determined for each bacterial strain. Afterwards, the mixed bacterial biofilms were treated with chestnut honey at different stages of maturity (incubation times: 2, 4, 6, 12, 24 h). The extent of biofilm inhibition was measured with a crystal violet assay and demonstrated by scanning electron microscopy (SEM). As the incubation time increased and the biofilm became more mature, inhibition rates decreased gradually. The most sensitive biofilm was the combination MRSA-S. epidermidis, with a 93.5% inhibition rate after 2 h of incubation. Our results revealed that chestnut honey is suitable for suppressing the initial and moderately mature stages of mixed biofilms.

The Transformation of a Person’s Life Plans in Mature Age

Introduction. There is a dual view about the orientation of transformation of life plans in people of mature age: on the one hand, the personality goes beyond the old structures, revealing its potential for the sake of further development; on the other hand, there is the risk of a motivational crisis associated with a change in the trajectory of personality development. Our research is designed to deepen and concretize ideas about the orientation and motivation of human life plans’ transformation at different stages of maturity. Purpose. The study of the peculiarities of motivation for the life plans’ transformation in mature age.Materials and methods. In this study, 4 tests were applied: The Diagnostics of Motivational Structure of Personality test by V. E. Milman, the Index of Aspirations test by E. Desi and R. Ryan (adapted by Yu. A. Kotelnikova), the Morphological Test of Life Values (MTLV) by V. F. Sopov, L. V. Karpushina, Test for Assessing the Life and Time Prospects of a Person by N. I. Nikolskaya. The Mann-Whitney U-test was used for statistical analysis of the obtained data.Results. The study involved 160 people aged 35–55. We found out that respondents’ motivational priorities differ in general orientation – representatives of early maturity are oriented externally, whereas the motivational orientation of older respondents is rather internal. The features of emotional experiences are also revealed: in early maturity, they are sthenic and characterized by productivity, and in later maturity, they are rather asthenic, hindering activity.Discussion. Our study expands existing information about the motivational and emotional aspects of the life plans’ transformation in early and late maturity. The motivational priorities of the representatives of both age cohorts are established. In general, maturity is associated with a redefinition of the activity vector, a revision of the significance of life goals, and a transformation of plans, which leads to an expansion or change in the boundaries of the subject or the actualization of asthenic emotional experiences.

Disaggregating the influence of maturity status on training, anthropometric, performance, skeletal periphery, and hormonal factors in athletic boys

Maturity offset (i.e., age from peak height velocity [PHV]) is widely used to assess maturational status among youth athletes, but details on the skeletal periphery, hormones and training factors are lacking. More precision is also needed to explicate the timing, tempo, and sequence of growth-related events. These gaps were addressed in a cross-sectional study. One hundred and two athletic boys (aged 14.1 ± 0.5 years) were evaluated for training details, salivary testosterone and cortisol, height, body mass, body mass index, body fat, fat-free mass (FFM), hand and carpal bone area, bone mineral content (BMC) and density (BMD), and countermovement jump performance. Participants were assigned to circa-PHV (N = 56) and post-PHV (N = 46) groups for comparisons, before data pooling and disaggregation using generalized additive and linear regression models. The older post-PHV group had a larger body size, more testosterone, and better performance and skeletal outcomes, whilst weekly training and school-based exercise favored the younger circa-PHV group (all p < 0.001). Smoother plots verified these differences via linear, or weakly non-linear, associations. Maturity offset was predicted (R2 = 0.848) by the linear combination of FFM (42.0 % relative contribution), hand BMD (31.5 %), chronological age (16.0 %), testosterone (6.7 %), and training hours (3.8 %). In conclusion, athletic boys who presented at different stages of maturity (-0.98 to 2.84 years from PHV) also differed on many developmental features. Most variables increased at constant, or near-constant, tempo with a higher maturity offset, with FFM and hand BMD emerging as the strongest linear predictors of maturational status.

Activity of enzymes in the follicular fluid and outcome of in vitro fertilization.

Defining certain parameters of the oocyte microenvironment that can be simply and quickly detected and that enable differentiation of oocytes that have better or worse quality could potentially increase the efficacy of the in vitro fertilization (IVF) method. Follicular fluid represents the microenvironment for the development of oocytes during different stages of maturation. Enzymes present in the follicular fluid may affect the quality of oocytes. There are inconsistencies in the literature concerning enzyme concentration in follicular fluid. The main aim of this study was to examine whether the concentration of enzymes aspartate aminotransferase, alanine aminotransferase (ALT), alkaline phosphatase, and lactate dehydrogenase in follicular fluid can influence the outcome of IVF. The study included 120 patients who were involved in the process of IVF. We used the follicular fluid obtained after follicular aspiration in the ovaries as biological material for the analysis. The values of enzymes LDL (method-kinetic UV test), aspartate aminotransferase (method-kinetic UV test), ALT (method-kinetic UV test) and alkaline phosphatase (method-colorimetric kinetic test) in the follicular fluid were determined on the analyzer АU 680, Becman Coulter. The criteria of the Istanbul consensus of clinical embryologists were used for the embryo assessment. The software package SPSS 20 was used for the statistical processing of data. The results of follicular fluid sample analysis showed a correlation between ALT concentration in the follicular fluid and IVF outcome. Based on our results, we can conclude that ALT concentration affects the outcome of IVF.

Physicochemical characterization, ethylene and carbon dioxide production at different stages of maturity of canistel

Physicochemical characterization, ethylene and carbon dioxide production at different stages of maturity of canistel

Erythroid variant evolving from chronic myeloid leukemia resistant to multiple tyrosine kinase inhibitors: a rare case report

BackgroundChronic myeloid leukemia (CML) is characterized by the presence of BCR::ABL1 fusion gene resulting from a reciprocal translocation, t(9;22)(q34;q11.2), leading to prominent granulocytic proliferation. The majority of patients initially present in chronic phase (CP), which may progress to advanced CML with predominantly granulocytic phenotypes in the absence of proper treatment or response to tyrosine kinase inhibitors (TKIs). We present an exceptionally rare case in which an erythroid variant emerged from a CML patient resistant to multiple TKIs. This variant is characterized by the detection of t(9;22) BCR::ABL1 fusion in erythroid precursors at various maturation stages and the absence of granulocytic progenitor hyperplasia typically seen in classical CML.Case presentationA 33-year-old female with CP-CML had received multiple TKI therapies since her initial diagnosis in 2015. Due to intolerable side effects and inconsistent adherence, she exhibited an inadequate response and developed new-onset pancytopenia. Bone marrow (BM) biopsy specimen revealed a hypercellular marrow with significant erythroid hyperplasia (90% of marrow cellularity) and a reversed myeloid-to-erythroid (M: E) ratio of 1:10. Both erythroid and myeloid cells displayed progressive maturation without dysplasia or excess blasts. Chromosomal analysis identified t(9;22) (q34;q11.2) in 19 out of 20 metaphase cells. BCR::ABL1 fusion transcript (p210 isoform) was confirmed by real-time quantitative polymerase chain reaction (RT-qPCR) and next-generation sequencing (NGS). Notably, no additional pathogenic cytogenetic abnormalities or ABL1 kinase domain mutations were detected. Here, we report the first published case of an erythroid variant emerging in a CML patient resistant to multiple TKIs—a distinct entity from the erythroid blast crisis evolving from CML.ConclusionThe erythroid variant of CML is distinguished by the presence of t(9;22) (q34;q11.2) BCR::ABL1 in predominant erythroid precursors at different stages of maturation. In a myeloid neoplasm showing predominant erythroid hyperplasia without typical CML features, it is vital to correlate morphology and t(9;22) BCR::ABL1 cytogenetic testing for accurate diagnosis, and to prevent confusion with PEL transformation in CML.

Label-free based comparative proteomics approach revealed the changes in proteomic profiles driven by different maturities in two Chinese white truffles, Tuber panzhihuanense and Tuber latisporum

T. panzhihuanense and T. latisporum are white truffle species native to China, of which T. panzhihuanense has significant commercial potential, with high nutritional value and unique flavor. Maturity is an important factor affecting the nutrition and aroma of truffles, which determines their economic status. Here, a label-free-based comparative proteomics method was used to determine the proteomic profiles of T. panzhihuanense and T. latisporum at two different stages of maturity. The results showed that both maturity and species significantly affected the protein expression patterns. T. panzhihuanense responded stronger to maturity than T. latisporum, accompanied by a more complex interaction network between proteins. Some critical proteins were regulated by maturity and variety, including those involved in aroma formation, e.g., S-adenosyl-methionine synthetase. The enrichment of oxidation–reduction processes, glycolysis, and SNARE interactions in vesicular transport were driven by species and maturity. This study provides the first insights into the proteomic profiles of T. panzhihuanense and T. latisporum, revealing the roles of key proteins and biological processes in their maturation.

Cardiomyocyte maturation alters molecular stress response capacities and determines cell survival upon mitochondrial dysfunction

Cardiomyocyte maturation during pre- and postnatal development requires multiple intertwined processes, including a switch in energy generation from glucose utilization in the embryonic heart towards fatty acid oxidation after birth. This is accompanied by a boost in mitochondrial mass to increase capacities for oxidative phosphorylation and ATP generation required for efficient contraction. Whether cardiomyocyte differentiation is paralleled by augmented capacities to deal with reactive oxygen species (ROS), physiological byproducts of the mitochondrial electron transport chain (ETC), is less clear. Here we show that expression of genes and proteins involved in redox homeostasis and protein quality control within mitochondria increases after birth in the mouse and human heart. Using primary embryonic, neonatal and adult mouse cardiomyocytes in vitro we investigated how excessive ROS production induced by mitochondrial dysfunction affects cell survival and stress response at different stages of maturation. Embryonic and neonatal cardiomyocytes largely tolerate inhibition of ETC complex III by antimycin A (AMA) as well as ATP synthase (complex V) by oligomycin but are susceptible to complex I inhibition by rotenone. All three inhibitors alter the intracellular distribution and ultrastructure of mitochondria in neonatal cardiomyocytes. In contrast, adult cardiomyocytes treated with AMA undergo rapid morphological changes and cellular disintegration. At the molecular level embryonic cardiomyocytes activate antioxidative defense mechanisms, the integrated stress response (ISR) and ER stress but not the mitochondrial unfolded protein response upon complex III inhibition. In contrast, adult cardiomyocytes fail to activate the ISR and antioxidative proteins following AMA treatment. In conclusion, our results identified fundamental differences in cell survival and stress response in differentiated compared to immature cardiomyocytes subjected to mitochondrial dysfunction. The high stress tolerance of immature cardiomyocytes might allow outlasting unfavorable intrauterine conditions thereby preventing fetal or perinatal heart disease and may contribute to the regenerative capacity of the embryonic and neonatal mammalian heart.

Maturation and antioxidant activity of 'Giombo' and 'Rama Forte' persimmons produced in the Brazilian semiarid.

The objective of this work was characterizing persimmons of the 'Giombo' and 'Rama Forte' cultivars harvested at different ripening stages in the Brazilian semiarid. Fruits were harvested at three ripening stages - green, semi-ripe and ripe - then evaluated for the following characteristics: fruit weight and diameter, skin and pulp color, fruit firmness, pulp pH, soluble solids content, titratable acidity, SSC/TA ratio, total soluble sugars, reducing sugars, astringency index, and the contents of tannin, vitamin C, carotenoid, β-carotene, and total extractable polyphenols. Also, total antioxidant activity by the DPPH and ABTS methods and pectin methylesterase, and polygalacturonase enzyme activities were evaluated. Two experiments were carried out in a completely randomized design, one for each cultivar, with treatments consisting of different stages of maturation, with five replications of three fruits each. Data were submitted to analysis of variance and the differences between the means were compared using the Tukey test at 5% probability. Fruit firmness and soluble solids content did not vary between maturation stages for any of the cultivars. However, the skin color index increased with advancing maturation for both 'Giombo' and 'Rama Forte'. The astringency index, the content of total extractable polyphenols, soluble tannins and the antioxidant capacity were lower in fruits harvested at the ripe stage, for both cultivars. It can be concluded that persimmons of the 'Giombo' and 'Rama Forte' cultivars present better physicochemical quality characteristics when harvested when ripe, with a totally yellow skin.

Comparison of Follicular Fluid Glycosaminoglycan and Hydroxyproline Concentration in Women with Polycystic Ovary Syndrome with Healthy Women

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies in women of childbearing age. As a complex structure, the extracellular matrix (ECM) and its components, including glycosaminoglycans (GAGs) and collagens, are highly affected by hormonal alterations. Objectives: This study aimed to evaluate the level of GAG and hydroxyproline in the follicular fluid (FF) of women with PCOS. Methods: Overall, 62 women referring to Fatemieh Hospital Infertility Center in Hamadan were evaluated in this study. FF samples were taken from 31 women with PCOS during ovarian puncture and 31 healthy women. Oocytes were evaluated in the different stages of maturation, namely, metaphase II (MII) and germinal vesicle (GV). Commercially available kits were used to measure the levels of glycosaminoglycans and hydroxyproline. Results: The results of the present study showed that the count of oocytes in women with PCOS was significantly higher than that in healthy women. Evaluating GAG and hydroxyproline levels indicated that their levels were meaningfully higher in the FF of women with PCOS. Conclusion: In general, the results of this study suggested that ECM changes in PCOS followed by alterations in the levels of GAGs and hydroxyproline may lead to an increase in the level of GAG and hydroxyproline in the FF. The evaluation of these markers may be the subject of further studies.

Germination and Vegetative Propagation of the Wild Species Cuphea pulchra Moric. (Lythraceae), a Potential Ornamental Crop

Cuphea pulchra Moric. is a species native to the Cerrado and Caatinga biomes that grows in environments with high temperatures and low rainfall and can be adapted as an ornamental plant for pots. Tests were carried out on C. pulchra seeds, as well as the cultivation of plants from both seeds and cuttings in a greenhouse. Seeds at different stages of maturity (green, almost ripe, and mature) were placed on agar and paper for germination tests. The cultivated plants were pruned as necessary. Two cutting tests were carried out according to the age of the donor plant. The flowering period was monitored. Germination was successful with the almost ripe seeds. Drastic pruning was able to produce compact plants in pots. Cutting tests had greater sprouting with younger donor plants. Cuphea pulchra stood out in terms of the length of the flowering period, which lasted up to ten months. Greenhouse cultivation produced viable plants for the ornamental plant market.

Side effects of X-ray irradiation on flight ability of Cydia pomonella moth.

The sterile insect technique (SIT) has proven to be an effective approach in managing the population of major invasive pests. Our previous studies showed that irradiation of Cydia pomonella males at a dosage of 366 Gy X-rays resulted in complete sterility. However, the mating competitiveness of sterilized males is significantly compromised, which can be attributed to a decline in their ability to fly. In this study, we examined the flight patterns of both male and female adults of C. pomonella. The results revealed significant variations in the average flight speed of both genders at different stages of maturity, with females displaying longer flight duration and covering greater distances. Effect of irradiation on the flight performance of 3-day-old male moths was further evaluated, as they demonstrated the longest flight distance. The findings indicated a significant decrease in flight distance, duration, and average speed, due to wing deformities caused by irradiation, which also limited the dispersal distance of moths in orchards, as indicated by the mark-and-recapture assay. Reverse-transcription quantitative polymerase chain reaction analysis revealed a down-regulation of flight-related genes such as Flightin, myosin heavy chain, and Distal-less following radiation exposure. These findings demonstrate that X-ray irradiation at a radiation dose of 366 Gy has a detrimental effect on the flight ability of male C. pomonella adults. These insights not only contribute to a better understanding of how radiation sterilization diminishes the mating competitiveness of male moths, but also aid in the development and improvement of SIT practices for the effective control of C. pomonella. © 2023 Society of Chemical Industry.

Assessment of the Effects of Roasting, Contact Grilling, Microwave Processing, and Steaming on the Functional Characteristics of Bell Pepper (Capsicum annuum L.).

Bell peppers (Capsicum annuum L.) in various stages of maturity are widely used in the diets of individuals and in the food industry; they are consumed both fresh and after thermal processing. However, every type of processing impacts the overall textural and bioactive characteristics of this plant-based food. In order to quantify the changes in the bioactive substances and color-structural characteristics that occur during selected heat treatments (contact grilling, roasting, roasting combined with microwaving, and steam cooking) of bell peppers at three maturity stages (green, yellow, and red), analyses of antioxidant activity, reducing sugar content, polyphenolic compound content, textural properties, and color coordinates in the L*a*b* system were carried out. Some of the processes used, such as contact grilling (15.43 mg GAE/g d.b.) and roasting combined with microwaving (15.24 mg GAE/g d.b.), proved to be beneficial as the total polyphenol content of green peppers (2.75 mg GAE/g d.b.) increased. The roasting (3.49 mg TE/g d.b.) and steaming (6.45 mg TE/g d.b.) methods decreased the antioxidant activity of yellow bell peppers (14.29 mg TE/g d.b.). Meanwhile, the roasting (0.88 mg Glc/g d.b.), contact-grilling (2.19 mg Glc/g d.b.), simultaneous microwaving and roasting (0.66 mg Glc/g d.b.), and steaming (1.30 mg Glc/g d.b.) methods significantly reduced the content of reducing sugars and reducing substances in red bell peppers (4.41 mg Glc/g d.b.). The studies proved that in order to preserve the antioxidant and bioactive properties of bell peppers, it is necessary to consider the use of appropriately selected heat treatments, depending on the different stages of maturity. The proper selection of adequate thermal treatment can not only increase digestibility, but also improve the bioavailability of bioactive substances from this raw material.