Different Periods Of Gestation Research Articles

Timing of dietary zinc additions during gestation for improved piglet survival.

The objectives of this study were to determine a practical approach to feeding elevated dietary zinc (Zn) to gestating sows in a commercial setting and to confirm preweaning mortality could be reduced by feeding high Zn to sows during different periods of gestation. The study was conducted at a commercial sow farm in the upper Midwest. Mixed parity sows (n = 267) over three consecutive weekly farrowing groups (sows farrowing within 1wk) were assigned randomly to one of the three dietary treatments within parity. Treatments consisted of: (1) control sows fed a corn-soybean meal diet containing 206mg/kg total supplemental Zn supplied by zinc hydroxychloride; (2) breed-to-farrow: as control + 147mg/kg supplemental Zn as ZnSO4 (353mg/kg total supplemental Zn) fed from 5 d after breeding to farrowing; and (3) day 110-to-farrow: as control fed from breeding to farrowing + 4,079mg/kg supplemental Zn as ZnSO4 (4,285mg/kg total supplemental Zn) starting day 110 of gestation until farrowing. At farrowing, individual piglets were weighed and identified within 12h of birth. Data were analyzed using PROC GLIMMIX of SAS and the model considered the fixed effect of dietary treatment and random effect of farrowing group. Dietary treatments did not affect number of total pigs born per litter. For breed-to-farrow sows, there was an increase in the percentage of pigs born alive compared to sows fed the control and day 110-to-farrow treatments (P < 0.001). The number of stillborn pigs expressed as a percentage of total litter size at birth decreased for breed-to-farrow sows (P < 0.001) compared with control or day 110-to-farrow sows. Mortality of low birth weight piglets from birth to weaning did not differ among dietary treatments (P = 0.305); however, a trend for decreasing post-natal mortality (P = 0.068) of normal birth weight pigs was observed for pigs born to sows fed elevated Zn 5 d before farrowing. In conclusion, feeding elevated Zn to sows throughout gestation increased the proportion of pigs born alive suggesting that elevated gestational Zn intake makes piglets more robust to endure the stresses of farrowing and decreases intrapartum mortality. Under the conditions of this study, elevated Zn intake of sows did not influence piglet post-natal survival. However, feeding high zinc throughout gestation may decrease piglet mortality during the parturition process.

Epidemiological analysis of congenital glaucoma: a national scenario.

Congenital glaucoma is a disease that involves increased intraocular pressure and can result in irreversible visual deterioration. The study of epidemiology allows the delineation of the characteristics associated with patients and specific risk factors. The objective of this study was to examine epidemiological trends, place of residence, duration of gestation, sex, and race of the newborn diagnosed with congenital glaucoma in Brazil. Data from SINASC (National Live Birth System) were used to analyze the period from 2017 to 2021 in Brazil. Linear regression and analysis of variance tests were employed to assess significance. The statistical significance was determined by p<0.05. A total of 47 cases of congenital glaucoma were identified in Brazil during the study period, with the highest incidence between the years of 2018 and 2021. The analysis of the distribution indicated that the states with the highest incidence were São Paulo, followed by Rio Grande do Sul and Pernambuco. Approximately 60% of cases occurred in male individuals, compared with 19 female cases. The ethnic analysis showed the highest incidence among whites and mixed. Regarding the length of pregnancy, statistical differences were observed between newborns of different periods of gestation. Infants born from pregnancies lasting between 28 and 31 weeks and 32 and 36 weeks were significant when analyzed with the group between 37 and 41 weeks. Studies on the mechanisms of congenital glaucoma seek to improve knowledge about the disease. Epidemiological evaluation is essential for identifying demographic and clinical patterns of the disease.

Effects of maternal calcium propionate supplementation on offspring productivity and meat metabolomic profile in sheep.

This study determined the effect of dietary calcium propionate (CaPr) as a source of energy supplementation during the First Half of Gestation (FMG), the Second Half of Gestation (SMG), and during All Gestation (AG), on offspring post-weaning growth performance, meat quality, and meat metabolomic profile. Thirty-one pregnant ewes were assigned to one of four treatments: a) supplementation of 30 gd-1 of CaPr during the first half of gestation (day 1 to day 75, n = 8) (FMG); b) supplementation of 30 gd-1 of CaPr during the second half of gestation (day 76 to day 150, n = 8) (SMG); c) supplementation of 30 gd-1 of CaPr during all gestation (AG, n = 8); d) no CaPr supplementation (control; CS, n = 7). The ewes were ad libitum fed a basal diet based on oat hay and corn silage. Ewes were distributed in a completely randomized unbalanced design to four treatments. The FMG group had lower (P ≤ 0.05) birth weight and weaning weight than the CS group. However, the average daily gain was similar across all treatments. Empty body weight and FMG had lower values (P ≤ 0.05) than the other groups. Both FMG and AG had lower hot carcass weight (P ≤ 0.05) compared to CS, while CaPr treatments resulted in reduced hot carcass yield (P ≤ 0.05). Meat color and texture were similar among treatments. A principal component analysis between gestation stages showed a trend for separating CS and FMG from SMG and AG, and that was explained by 93.7% of the data variability (PC1 = 87.9% and PC2 = 5.8%). Regarding meat metabolomic profile, 23 compounds were positively correlated between all treatments. Only 2 were negatively correlated (eicosane and naphthalene 1,2,3); but tetradecanoic acid, hexadecane, undecane 5-methyl, (-)-alpha, hexadecenoic acid, octadecanoic acid, and octadecane had a highly significant correlation (P ≤ 0.05). Overall, dam supplementation with CaPr during different periods of gestation provoked changes in meat metabolites related to the biosynthesis of fatty acids in lambs without negative changes in lamb's growth performance and carcass quality.

The influence of the coronavirus disease transmitted during pregnancy on the course of gestation and changes in the main biotopes of a woman’s organism

The article presents literature data on the peculiarities of the pregnancy course and changes in the main biotopes in women who suffered from COVID-19 in different periods of gestation. Certain regularities regarding the association of perinatal complications with the severity of the disease, the presence of somatic and gynecological pathology in these pregnant women, in particular, obesity and chronic inflammatory diseases of the genital organs, were observed.According to research conducted on the basis of the department of pathology of pregnancy and childbirth of the State Institution «Institute of Pediatrics, Obstetrics and Gynecology named after academician O. M. Lukyanova of the National Academy of Sciences of Ukraine», the risk group of a severe course of the coronavirus disease includes all the same factors as for any other diseases, not related to pregnancy: age over 35 years; the presence of concomitant pathology (diabetes, obesity, hypertension), work, lifestyle or housing conditions that increase the risk of infection. More frequent manifestations of obstetric pathology were found in older women and primipara women.Complicated obstetric anamnesis, namely, medical abortions and miscarriages, cervical pathology, previous inflammatory diseases, especially of the genital organs, are considered to be risk factors. The course of pregnancy and childbirth are accompanied by such complications as the threat of early miscarriage; anemia; combination of placental dysfunction, polyhydramnios and fetal distress; anomalies uterine activity, which increases the frequency of pathological childbirth in this contingent of women and requires repeated hospitalization during pregnancy.A review of scientific publications in recent years demonstrates the significant impact of COVID-19 on the composition of biotopes of the human organism. There are convincing data on the close relationship between the infection of the mucous membranes of the upper respiratory tract and the infection of the lungs with the SARS-CoV-2 virus. Lung hypoxia, typical for this infection, causes the growth of anaerobes and facultative anaerobes, which are part of the mentioned microbiomes during a person’s life. A significant number of observations have found the correlation of changes in the microbiocenosis of the respiratory tract and intestines in patients with SARS-CoV-2 and convalescents. At the same time, there is also a disorder in the vaginal microbiocenosis after the coronavirus disease, especially during pregnancy. A conclusion was made regarding the correlation between the severity of COVID-19 course and the number of lactobacilli in the vaginal biotope: the more severe course, the worse of the microflora composition. The gestation period is more often complicated by the threat of early pregnancy loss, placental dysfunction, polyhydramnios, fetal distress, and the threat of premature birth in those women who suffered a coronavirus infection in the I trimester. A large rate of polyhydramnios in most cases indicates the risk of intrauterine infection, so such women should be carefully monitored both for the impact of COVID-19 and for the activation of latent infections in the background of reduced immunity and, first of all, violations of the vaginal biotope. At the same time, a high frequency of obesity was found among these women, which worsens the course of infectious processes and the condition of the main biotopes.Therefore, for this contingent of women, the issue of timely detection and correction of violations of the genital tract microbiocenosis as a prevention of perinatal and obstetric complications becomes relevant. In order to avoid the negative impact of changes in the vaginal microbiocenosis on the condition of a woman and her child in the future, modern universal and safe preparations during pregnancy and lactation should be used for local treatment and strengthening of regenerative processes, which do not contain an antibacterial component, do not cause antibiotic resistance and are effective, available and convenient to use. The clinical experience of recent years allows to recommend for wide use in obstetrics and gynecology the complex safe and effective local preparations, which include chlorhexidine, chlorophyllipt and preparations with hyaluronic acid and plant extracts.

PROGESTERONE RECEPTOR GENE POLYMORPHISM AS A FACTOR IN PRETERM BIRTH (LITERATURE REVIEW)

The purpose of the work – to analyze data from the world literature regarding thedetermination of the probable role of the progesterone receptor gene polymorphism asa factor in the occurrence of premature birth.Conclusions. 1. Progesterone receptor gene polymorphism plays a significant role inthe mechanisms of development and course of miscarriage, negative pregnancy outcomeand low birth weight of newborns. 2. The probability of the threat of premature birthin different periods of gestation or termination of pregnancy with premature birth maydepend on the presence of one or another polymorphism. 3. Genetic variability thatcan lead to changes in the biological functions of the progesterone receptor, the geneticpolymorphism of the progesterone receptor is a major risk factor for complications ofpreterm birth.

Thyroid Function at Age Fifty After Prenatal Famine Exposure in the Dutch Famine Birth Cohort.

BackgroundEarly-life exposures during gestation may permanently alter thyroid physiology and health in adulthood. We investigated whether exposure to the Dutch Famine (1944-1945) in late, mid, or early gestation influences thyroid function (i.e., incidence of thyroid disease, thyroid autoantibodies, thyroid stimulating hormone (TSH), and free thyroxine (FT4) levels) in adulthood. We specifically assessed whether potential effects of famine differed for men and women.MethodsThis study includes 910 men and women born as term singletons in the Wilhelmina Gasthuis in Amsterdam, the Netherlands, shortly before, during, or after the Dutch Famine. We evaluated medical histories for previous diagnosis or current treatment for thyroid dysfunction. At age 50 blood samples were drawn from 728 individuals for tests of thyroid function. We studied the prevalence of overt hypo- and hyperthyroidism and thyroid autoimmunity using medical histories, and measurements of TSH, FT4, anti-TPO and anti-TG, comparing participants exposed to famine at different pregnancy trimesters or born before or conceived after the famine. Additionally, we studied associations of TSH and FT4 levels with in utero famine exposure in a subsample of men and women free of thyroid disease that were exposed in late, mid, or early gestation.ResultsThere were no differences in thyroid dysfunction diagnosis or current treatment between participants at age 50 years who been exposed to famine during different periods of gestation and those born before or conceived after. There was no association between famine exposure and overt hypo- or hyperthyroidism or thyroid autoantibody positivity. Women who had been exposed to famine in mid gestation had slightly lower TSH levels than women who had not been exposed to famine prenatally (b=-0.06; 95%; CI=[-0.11,-0.02]; p<0.01). No differences in TSH levels were observed in men, and no differences in FT4 levels were observed in men or women.ConclusionsThere are no differences in adult thyroid disease at age 50 years according to prenatal famine exposure. However, the lower TSH levels in women exposed to famine in the second trimester suggest that there may be sex-specific effects of famine exposure during a critical period of thyroid development on hypothalamic-pituitary-thyroid axis regulation in adulthood.

The effect of prenatal hypoxia on the manifestation of the ventilatory reaction of Wistar rats upon presentation of a hypoxic stimulus

The aim of the scientific work was to study the multidirectional effect of hypoxic sessions presented to pregnant rats at different periods of gestation on the manifestation of the ventilatory reaction of their offspring with dosed hypoxic hypoxia.Materials and methods: a plethysmograph were used to record respiratory volume, respiratory rate and calculate the minute volume of respiration of intact rats (n=77) of the Wistar breed.Results and discussion: presentation of a hypoxic stimulus to pregnant rats on certain days of gestation did not affect the gestation period, body weight and number of offspring, but changed the ventilatory response to hypoxia in males born and reached three months of age. Prenatal hypoxia, presented in the middle of pregnancy, led to a decrease in the ventilatory response to lack of oxygen in the offspring, and exposure of pregnant rats in hypoxic conditions at the end of the second trimester increased the ventilatory response to hypoxia.

Оцінка ефективності відеолапароскопічної апендектомії у вагітних

The purpose of the study is to assess the efficiency results of treatment of acute appendicitis in pregnant women by using laparoscopy at different periods of gestation on the basis of studying the effect of increased intra-abdominal pressure due to carbon dioxide pneumoperitoneum on the course of pregnancy, the state of the uterus and fetus, as well as improving the technique of performing the operation. Materials and methods. 73 pregnant women with acute appendicitis were examined. The average age of the surveyed was 25.9±0.7 years, ranged from 17 to 42 years. The gestation period ranged from 4-5 to 37-39 weeks. There were 26 (35.6%) pregnant women in the first trimester, 32 (43.8%) – in the second trimester, and 15 (20.5%) – in the third trimester. The intervention was performed using the Olympus OTV-SC endoscopic complex. Pneumoperitoneum with CO2 was created about 10-12 mm Hg. In all women, the parameters of body temperature and blood pressure were studied, and the postoperative restoration of the motor-evacuation function of the gastrointestinal tract was assessed. Laboratory research included the study of individual indicators of the leukocyte formula, changes in the hemostasis system and microbial contamination of tissues of trocar wounds. The results of the effect of increased intra-abdominal pressure during laparoscopy on the state of the fetus and uterus in the early postoperative period were assessed using the BMT-9141 (Germany) and FM-7 (England) fetal monitor in women with a gestational age of 32 - 39 weeks. Results and discussion. The study showed that laparoscopy is possible with good results in the treatment of acute appendicitis in pregnant women in the first, second and third trimesters but with few exceptions. The technical features of performing laparoscopy in pregnant women are associated with the increased size of the uterus during the development of pregnancy. When substantiating therapeutic measures for endoscopic interventions based on the study and assessment of signs of systemic inflammatory response syndrome like temperature reaction, individual indicators of leukocyte count, peripheral hemodynamics, as well as restoration of dysfunctions of the gastrointestinal tract, the revealed advantages of laparoscopic appendectomy. Removal of the appendix from the abdominal cavity in plastic containers reduced microbial contamination of wounds, leading to a decrease in the number of postoperative complications. Conclusion. Laparoscopic interventions are effective in acute appendicitis in pregnant women. They provide a mild intoxication syndrome, rapid physical rehabilitation of patients. Their advantages are the reduction of manifestations of uterus and fetal disorders, rapid postoperative recovery of gastrointestinal tract functions

Role of vaginal progesterone on uterine artery Doppler changes in pregnant women at risk of preterm labor

BackgroundProgesterone is increasingly being used in women at high risk for preterm labor and for maintenance tocolysis. Ultrasound evaluation of uterine in pregnant women in the second trimester of pregnancy can be a predictor of birth complications.ObjectiveTo study the effect of vaginal progesterone on uterine by doppler sonography among women in the second and third trimesters of pregnancy who are at risk of preterm labor.Patients and methodsA prospective study was carried out on 60 patients attending Obstetrics and Gynecology Department, Menoufia Teaching Hospital, Menoufia, Egypt, during the period from March 2019 to March 2020. The patients were admitted to the hospital for preterm labor, which was arrested by acute tocolysis within 48 h. All participants were subjected to routine baseline investigations and Doppler flow assessment.ResultsThere was a highly statistically significant difference (P < 0.001) in uterine artery pulsatility index (PI) before and after treatment (P = 0.000). Uterine artery PI decreased significantly after treatment (0.817 ± 0.16) than before treatment (0.969 ± 0.27). Uterine artery PI decreased significantly after treatment than before treatment in different periods of gestation (weeks). Moreover, there was no statistically significant difference (P>0.05) between the studied women as change in umbilical artery PI before and after treatment in different periods of gestation (weeks). Moreover, uterine artery PI was significantly correlated (P < 0.05) with period of gestation (week) before and after progesterone treatment.ConclusionUterine artery pulsatility index (PI) decreased significantly after progesterone treatment than before treatment in different periods of gestation (weeks). Umbilical artery PI and middle cerebral artery Doppler PI were not significant different among the studied patients before and after progesterone treatment in different periods of gestation (weeks). Uterine artery PI and middle cerebral artery Doppler PI were negatively associated with period of gestation (week) before and after progesterone treatment.

Морфофункциональные изменения яичек плода человека при внутриутробной гипоксии

Introduction. Intrauterine hypoxia is one of the most frequent complications in pregnancy. It is one of the risk factors of fetal development disorders. In the antenatal period, the effects of hypoxia on re-productive system can impair fetus development and lead to negative consequences. The aim was to studypathological and functional features of testes development in different periods of gestation with chronic intrauterine hypoxia. Materials and methods. We examined autopsies of testicular tissues of 78 fetuses deceased at gestation weeks 19–40. Histological and immunohistochemical methods (using antibodies to Ki-67, Bcl 2, AR, ER, and FGF) were employed. Results. Morphometric examination showed a decrease in the number of tubules, cells in the tubules, and the area of the tubules with a significant increase in the stromal area. Immunohistochemistry revealed a weak expression of proliferation and apoptosis marker, a pronounced diffuse expression of estrogen receptor, and a weak expression of androgen receptor. Fibroblast growth factor expression was increasing up to week 29 and then sharply decreased. Conclusion. Chronic hypoxia leads to damage of germ cells and gland atrophy in the testicles of fetuses. Prolonged oxygen deprivation in the testicular tissue triggers compensatory mechanisms, which manifest in the apoptosis inhibitor expression. Hypoxia disrupts proliferation processes and reduces the functional activity of interstitial endocrinocytes, which is confirmed by the weak AR expression and pronounced ER expression during all gestation periods. These structural changes in testicular morphogenesis may lead to impaired fertility. Keywords: hypoxia, testicle, immunohistochemistry, fertility disorders, antenatal period

ПЕРИНАТАЛЬНАЯ ОХРАНА ПЛОДА И НОВОРОЖДЕННОГО. ВЛИЯНИЕ ВРЕДНЫХ ФАКТОРОВ НА ПЛОД

The lecture discusses the tasks of perinatology, the influence of damaging factors at different periods of gestation, types of damaging effects, perinatal diagnosis and prevention of risks of fetal damage.

Transabdominal ultrasonographic determination of pregnancy and fetal viability in buffalo cows

The study was conducted to evaluate the application of transabdominal ultrasonography for the determination of pregnancy and fetal viability in Iraqi buffaloes. Thirty-two buffaloes at different periods of gestation were included. The buffaloes were palpated transrectally and transabdominal ultrasonography was applied to determine the pregnancy and fetal viability status. The accuracy, sensitivity, specificity, the positive and negative predictive value of transrectal palpation were 93.7, 100, 83.3, 90.9, and 100%, and for transrectal palpation were 78.1, 100, 58.8, 68.2, and 100%, respectively. The values of accuracy and specificity in the first trimester obtained by transabdominal ultrasonography were lower than those obtained by rectal palpation (66.6 vs. 94.4% and 62.5 vs. 90.9%), whereas the same values of accuracy and specificity were at second and third trimesters. Notwithstanding the values of the transrectal palpation and transabdominal ultrasonography sensitivity were 100% in the three trimesters of buffalo’s gestation. At the first trimester of gestation, transabdominal ultrasonography required more time in comparison to transrectal palpation to get the final decision. Conversely, transrectal palpation required more time than transabdominal ultrasonography to diagnose the pregnant buffalos which were at the second and third trimesters of gestation. The transabdominal ultrasonography pregnancy diagnosis in the third trimester was faster than at the second and first trimester, a highly significant (P<0.001) negative correlation was reported between the time required for pregnancy diagnosis and the gestation age. It can be concluded that the transabdominal is a practical approach of ultrasonography for pregnancy diagnosis and determination of fetal viability in buffalo cows especially at the second and third trimesters of gestation.

Frequent Placental SARS-CoV-2 in Patients with COVID-19-Associated Hypertensive Disorders of Pregnancy

Introduction: Studies described an increased frequency of hypertensive disorders of pregnancy (HDP) after a COVID-19 episode. There is limited evidence about SARS-CoV-2 viral load in placenta. This study aimed to investigate the relationship between SARS-CoV-2 viral load in the placenta and clinical development of HDP after COVID-19 throughout different periods of gestation. Methods: This is a case-control study in women with and without gestational hypertensive disorders after SARS-CoV-2 infection diagnosed by RT-PCR during pregnancy. Patients were matched by gestational age at the moment of COVID-19 diagnosis. We performed an analysis of SARS-CoV-2 RNA levels in placenta. Results: A total of 28 women were enrolled. Sixteen patients were diagnosed with COVID-19 during the third trimester and the remaining 12 patients in the other trimesters. Ten placentas (35.7%) were positive for SARS-CoV-2, 9 of them (9/14, 64.3%) belonged to the HDP group versus 1 (1/14, 7.2%) in the control group (p = 0.009). Those cases with the highest loads of viral RNA developed severe preeclampsia (PE). Conclusion: Among women diagnosed with COVID-19 during pregnancy, the presence of SARS-CoV-2 in the placenta was more frequent among women suffering from PE or gestational hypertension. Furthermore, the most severe cases of HDP were associated with high placental viral load, not necessarily associated with a positive nasopharyngeal RT-PCR at delivery. Our data suggest that SARS-CoV-2 infection during pregnancy could trigger gestational hypertensive disorders through persistent placental infection and resulting placental damage.

Gestational weight gain trajectories in GARBH-Ini pregnancy cohort in North India and a comparative analysis with global references.

To describe the pattern of gestational weight gain (GWG), derive reference centiles for GWG specific to North Indian population, and to compare the weight gain across different periods of gestation with the INTERGROWTH-21st reference. A prospective pregnancy (GARBH-Ini) cohort was initiated and followed between May 2015 and June 2019 in a district hospital, Gurguram, North India. GWG centile curves were modelled by Generalized Additive Models for Location, Scale and Shape method (n = 2844) and compared with INTERGROWTH-21st reference. The independent association of GWG with biological and social predictors was assessed using multivariable regression analysis. Percentiles (3rd, 10th, 50th, 90th and 97th) for each completed week from 18-40 weeks of gestation were derived from smoothed centile curves. The median GWG across pregnancy during specific antenatal visits was 1.29 at 18, 4.44 at 26, 5.8 at 30 and 9.06 kg at 40 weeks of gestation. Nearly 26% of participants had GWG < 10th centile at 18-20 weeks as per INTERGROWTH-21st reference and this increased to 45% at delivery. Significant predictors of GWG included maternal age, height, first trimester body mass index, parity, type of family, and use of clean fuel for cooking. These GWG percentiles will serve as a useful reference, particularly during the WHO recommended antenatal visit schedule for optimum pregnancy outcomes, for clinicians and researchers. Multiple independent biological and social predictors of GWG suggest that single interventions are unlikely to bridge the gap between general Indian population and international references.

The influence of birth weight and gestational age on kidney function in premature infants

Introduction. The impact of prematurity on the functional state of the kidneys in infants has not yet been sufficiently studied. Aim. To determine the influence of birth weight and gestational age on the creatinine level in the blood and glomerular filtration rate (GFR) in early childhood. Materials and methods. A retrospective analysis was conducted on medical records of 316 children aged from 1 month to 1.5 years, hospitalized at the Department of Early Childhood Pathology (National Medical Research Center for Children’s Health, Moscow) from 2012 to 2020 due to consequences of perinatal CNS damage. Children without congenital kidney diseases, with normal urine values in medical history, without structural abnormalities on ultrasound were included in this study. Serum creatinine was determined by the enzymatic method, GFR - by the Schwartz’s formula using a coefficient of 0.413, as well as, previously proposed coefficients of 0.33 for premature and 0.44 for full-term infants. Results. In premature infants, notably born with extremely low birth weight and very low birth weight, at the age of 1 year, serum creatinine is reduced compared to full-term infants, GFR in deep-premature infants exceeds the level of GFR in full-term infants by the year. The results allow concluding the method of calculating GFR by formulas based on serum creatinine to be invalid. Due to possible hyperfiltration in preterm infants, they need regular monitoring urine tests, blood pressure, due to the risk of developing chronic kidney disease. Conclusions. It is necessary to search for other methods for determining GFR in extremely premature infants. The established indices of the blood creatinine content can be used as reference values for different periods of gestation and body weight at birth in institutions using the enzymatic method for determining blood creatinine. The obtained GFR indices as a reference can be recommended for full-term and premature babies born after 32 weeks of gestation and with a birth weight of more than 1500 g.

FEATURES OF THE CLINICAL COURSE OF PREGNANCY, CHILDBIRTH IN WOMEN WITH COVID-19 AT DIFFERENT PERIODS OF GESTATION

The study of the features of the clinical course of pregnancy, childbirth in women who have had COVID-19 at different periods of gestation is relevant, especially in the context of a modern pandemic. Purpose of work. To determine the features of the clinical course of pregnancy, childbirth in women with COVID-19 at different periods of gestation. Materials and methods. 57 women who were ill with Covid-19 at different stages of pregnancy were examined. Results and discussion. The peculiarities of the clinical course of pregnancy, childbirth in women who have undergone Covid-19 diseases in the 1st trimesterinclude the syndrome of threatened abortion (73.6 %), placental dysfunction (100%), fetal growth retardation syndrome (42.9 %); in the second trimester - syndrome of threatened premature birth (55.6 %), placental dysfunction (100 %), amniotic fluid pathology (63.2 %); in the third trimester - anemia - in 100 %, antenatal fetal distress - in 42.1 %, pareclampsia - in 47.4 %, pathological blood loss - in 26.3 %. Conclusions. The course of pregnancy and childbirth in women who have undergone COVID-19 diseases in different trimesters of gestation have certain clinical features. Gestational complications in women who were ill in the early stages of the first trimester include the syndrome of threatened abortion, spontaneous abortion, missed abortion, premature birth in the second trimester, fetal growth retardation syndrome in the second and third trimesters. The gestational complications in women who were ill in the second trimester include the syndrome of threatened abortion, premature birth, pathology of amniotic fluid (amniotic fluid of «black» color), the phenomenon of angiitis in the abdominal cavity, placental dysfunction, fetal growth retardation syndrome, distress fetus, antenatal fetal death. The gestational complications in women who were ill in the third trimester include the syndrome of threatened premature birth, placental dysfunction, and fetal distress.

Pathomorphological changes of the placenta in coronavirus disease (COVID 19)

The aim of this research was to study the pathomorphological changes in the placenta in case of coronavirus disease (COVID-19) in the anamnesis during different periods of gestation. Materials and methods. 53 placentas of women with the coronavirus disease (COVID-19) (RNA determination of the SARS-CoV-2) during the pregnancy were studied. The material was divided into the following groups: 1 and 2 – placentas in cases with a negative PCR test in full-term newborns who were born in satisfactory condition and high Apgar score. Group 1 (n = 29) – mother’s history of COVID-19 at 34–39 weeks of gestation; group 2 (n = 17) – mother’s history of COVID-19 at 23-33 weeks of gestation; 3 (n = 7) – placenta in case of antenatal fetal death. Results. Statistically significant differences were revealed between study groups: chorioamnionitis prevailed in group 1 (in 28 cases (96.6 %); confidence interval (CI): 86.4–100.0 %; P1-2 = 0.004); in groups 2 and 3 – arteriosclerosis (in 13 cases (76.5 %); CI: 52.2– 93.9 %; P1-2 = 0.0003 and in 7 cases (100 %); CI: 75.7–100.0 %; P1-3 = 0.001; respectively). COVID-19 was diagnosed in group 1 from 34 to 39 weeks of gestation (median 36.5), group 2 from 23 to 33 weeks (median 28.0), in group 3 from 13 to 32 weeks with a median of 24.5. Antenatal fetal death was observed between 14 and 41 weeks (median 31.4). Conclusions. Pathomorphological changes in the placenta in the coronavirus disease COVID-19 depended on the duration of the post-COVID interval (the time interval from the diagnosis of COVID-19 and the moment of delivery), due to the sequential change in the phases of the inflammatory process: alteration, exudation and proliferation, followed by fibrosis. The formation of acute placental insufficiency in coronavirus disease COVID-19, diagnosed up to 1–6 weeks to delivery, is associated with the development of severe disorders of circulation and acute exudative inflammatory reactions of varying severity. The formation of chronic placental insufficiency is associated with the proliferative stage of inflammation. These changes lead to the development of fibrosis in the wall of arterioles and intervillous space. The most significant structural changes in the placenta, leading to placental insufficiency, were observed in group 2 – at the time of infection in the period from 23–33 weeks of pregnancy. Coronavirus disease COVID-19 in the mother in the second trimester of pregnancy is a risk factor for perinatal losses, which are caused by the changes in the placenta described above with an increase in the duration of the post-COVID interval.

Systematic review and meta-analysis of vitamin D deficiency in different pregnancy on preterm birth: Deficiency in middle pregnancy might be at risk.

Background:Current studies suggest that vitamin D deficiency during pregnancy can produce a certain effect for preterm birth (PTB), but there is no research showing whether vitamin D deficiency has a consistent effect in different pregnancies; thus, we conducted a systematic review and meta-analysis of 24 observational studies, grouping them according to the gestational age at the time of serum sampling, to investigate whether vitamin D deficiency in different periods of gestation has different effects on PTB and to provide an evidence-based basis for pregnant women to measure and supplement vitamin D.Methods:The databases PubMed-Medline, EMBASE, the Cochrane Library, Web of Science, EBSCO, CBM, and CNKI were searched until February 2020. Two researchers independently assessed the eligibility and quality of studies, and STATA 12.0 software was used for meta-analysis.Result:Seven cohort studies, 13 case–control studies, and 4 cross-sectional studies were included from 2500 articles by inclusion and exclusion criteria. After adjusting for age, race, and other confounding factors, meta-analysis results showed that vitamin D deficiency in the first trimester, the second trimester, and the third trimester did not increase the risk of PTB (odds ratio (OR) = 1.01, 95% confidence interval (CI) (0.88, 1.16), P = .867; OR = 1.12, 95%CI (0.92, 1.37), P = .249; OR = 1.05, 95%CI (0.87, 1.27), P = .602). However, there was moderate heterogeneity in the study of vitamin D deficiency in the second trimester, and subgroup analysis suggested that vitamin D deficiency in the second trimester may increase the risk of PTB (OR = 1.33, 95%CI (1.15, 1.54), P = .000). A sensitivity analysis of the second trimester showed that excluding any 1 study did not significantly change the results.Conclusions:Vitamin D deficiency in early and late pregnancy may not be associated with PTB, while vitamin D deficiency in middle pregnancy is likely to have an important effect on PTB. Vitamin D levels should be measured in the second trimester of pregnancy, and vitamin D supplements should be provided if necessary.

Growth performance and linear body measurements of gestating rabbit subjected to varying level of feed restrictions

To optimize production and ensure sustainability, there is need for judicious management of the resources employed in livestock production enterprise. For inefficient utilization of resources can jeopardize or hamper livestock production and availability. This present study was conducted to determine the performance and changes in morphological parameters of rabbit does subjected to quantitative feed restriction at different periods of gestation. A total of seventy-two rabbits does with initial live weight range of 1.7-2.0 kg were employed in this study. The study was arranged in a 3x3 factorial layout. The rabbits were divided into nine treatments of eight does per treatment and exposed to three levels of feed restrictions (0, 15, 30%) at different gestation periods (15-19, 20-24, 25-29 days). Water was supplied ad libitum throughout the experimental period. Data were collected and documented for analysis on feed intake, weight gain, changes in linear body parameters and gestation length. Significant (p&lt;0.05) differences were noted for final weight, weight gain, feed gain, feed intake and gestation length. Restriction at 15% recorded highest final and weight gain. Longest gestation length was recorded for does on 30% restriction. Interaction between restriction and periods of gestation shows significant (p&lt;0.05) differences in weight gain, feed gain, feed intake and gestation length. All the morphological parameters considered in this present study were not influenced (p&gt;0.05) by restricted feeding and periods of feed restriction. Therefore, it can be concluded that 15% feed restriction could be applied at 20-24 days of gestation without any adverse effect on maternal well-being.

Correction: Vaginal microbiome analysis of healthy women during different periods of gestation.

Correction: Vaginal microbiome analysis of healthy women during different periods of gestation.