Different Districts Of Punjab Research Articles

Geospatial Dynamics of SARS-CoV-2 Variants during the Fifth Wave of COVID-19 in Punjab, Pakistan

Background: The study was conducted in the various districts of Punjab, Pakistan, to ascertain the incidence and epidemiology of SARS-CoV-2 variants circulating in the population during the fifth wave of COVID-19.Method: A total of 9603 nasopharyngeal swab samples of suspected patients were collected from the different districts of Punjab from December 2021 to April 2022. In the BSL-3 facility, an auto-extractor (Uni-medica) was used to extract the RNA genome. Viral detection and quantification were performed using real-time reverse transcriptase PCR. Multiplex PCR was used to target different mutations of the spike protein in order to identify SARS-CoV-2 variants.Results: 711 samples were found to be positive from a total of 9603. The Omicron variant of concern (VOC) was the predominant lineage of SARS-CoV-2 circulating at the time of sampling. Ninety-one percent proportion of COVID-19 was caused by the Omicron, followed by the wild variant (3.80%) and Delta (68.11%). Men were found to have a greater Omicron prevalence (47.96%) than women (42.05%). Furthermore, compared to older individuals (32.07%) and younger kids (10.55%), adults had the higher percentage of Omicron (47.39%).Conclusion: This study brought attention to the Omicron variant's circulation in Punjab Province, Pakistan, during the COVID-19 fifth wave.Keywords: Coronavirus; Omicron; Delta; Variant of Concern; Multiplex PCR; Punjab; Pandemic

Niche partitioning and competition between different rabbit breeds using stable isotopes

Stable isotope analysis (SIA) is an evolving method for determining diet, understanding food web and resolving biogeochemical issues in the ecosystem. This study aims to trace out ecological niche preferences/partitioning and competition among the lagomorphs, including two different breeds of European rabbit (Oryctolagus cuniculus), New Zealand rabbit and American Dutch rabbit, using SIA. Thirty-two samples of tooth enamel were analysed, which were collected from different districts of Punjab, Pakistan, including Okara, Sahiwal and Kasur. Among these samples, 16 belonged to the New Zealand breed (08 male and 08 female rabbits) and 16 to the American Dutch breed (08 male and 08 female rabbits). Significant (P<0.001) intergender differences in the isotope content of δ13C in the enamel for New Zealand and American Dutch rabbit were found. The European rabbits showed significant differences for both genders in the stable isotope of oxygen in the enamel (δ18O) values (P=0.05). Nitrogen stable isotope results showed no significant intergender differences between American Dutch and New Zealand rabbits (P=0.24). The stable isotope results for δ13C, δ15N, and δ18O indicate that the trophic niche partitioning of both breeds overlaps, which can potentially cause competition for resources, whereas the water intake may differ among different genders, which may reflect differential gender-related activities. The archaeological and fossilised data of lagomorphs is present, but there is no significant literature available for living lagomorphs (rabbits). In general, this study provides a basic and first dataset for δ13C, δ15N, and δ18O of living lagomorphs, which can serve as a comparative dataset for future studies.

Molecular detection of Coxiella burnetii in raw meat samples collected from different abattoirs in districts Kasur and Lahore of Punjab, Pakistan.

Coxiella burnetii is the zoonotic pathogen that causes Q fever; it is widespread globally. Livestock animals are its main reservoir, and infected animals shed C. burnetii in their birth products, feces, vaginal mucus, urine, tissues, and food obtained from them, i.e., milk and meat. There were previously very few reports on the prevalence of C. burnetii in raw meat. This study aimed to determine the prevalence of C.burnetii and its molecular characterization in raw ruminant meat from the Kasur and Lahore districts in Punjab, Pakistan, as this has not been reported so far. In this study, 200 meat samples, 50 from each species of cattle, buffalo, goat, and sheep, were collected from the slaughterhouses in each district, Kasur and Lahore in 2021 and 2022. PCR was used for the detection of the IS1111 element of C. burnetii. The data were recorded and univariate analysis was performed to determine the frequency of C. burnetii DNA in raw meat samples obtained from different ruminant species using the SAS 9.4 statistical package. Of the total of 200 raw meat samples, C. burnetii DNA was present in 40 (20%) of them, tested by PCR using the IS1111 sequence. The prevalence of C.burnetii differed among the studied species of ruminants. When species were compared pairwise, the prevalence in cattle was statistically significantly lower than in sheep (P = 0.005). The sequence alignment based on origin implied that the strains are genetically diverse in different districts of Punjab, Pakistan. The findings demonstrated that the prevalence of C. burnetii, especially in raw meat samples, deserves more attention from the health care system and professionals from Punjab, Pakistan, i.e., abattoir workers and veterinarians.

Heavy metals contamination in vegetables irrigated with wastewater: a case study of underdeveloping regions of Pakistan.

Irrigation of food crops with untreated wastewater leads to the accumulation of heavy metals in their edible parts. Exposure to toxic elements through consumption of contaminated vegetables even at very low concentration is becoming a serious problem for human health in many countries. This issue of heavy metals contamination needs great attention especially in Pakistan where irrigation by wastewater has become a common practice in urban and periurban areas. The present study was conducted to assess the contamination of different heavy metals including nickel (Ni), cadmium (Cd), lead (Pb) and chromium (Cr) in vegetables irrigated with wastewater in different districts of Punjab (Pakistan) like, Faisalabad, Lahore, Gujranwala, Sargodha, Sahiwal, Multan, Rawalpindi and Bahawalpur during years 2016-2019. The maximum contamination factor (Ni, 1.39; Cd, 5.86; Pb, 3.99; Cr, 2.24), pollution load index (2.92) and degree of contamination (13.48) were observed in wastewater irrigated agricultural soils of district Gujranwala as compared to other districts. Mean metal concentration in vegetables grown with untreated wastewater and transfer factor varied with plant type, frequency of irrigation and soils types/series of different districts of Punjab. The highest metal contamination was observed in Gujranwala where 44% vegetable samples were contaminated with Ni, 87% with Cd, 97% with Pb and 88% with Cr. Radar analysis and hierarchical cluster analysis showed that mustard leaves, spinach and lettuce were more contaminated vegetables compared to other vegetables. This study contributes to the evidence-based conclusion that cultivation of vegetable with untreated wastewater should be prohibited and required treatment of wastewater is needed.

Availability and readiness of primary healthcare facilities for the management of non-communicable diseases in different districts of Punjab, Pakistan.

Non-communicable diseases (NCDs) and their effects are rising quickly. NCDs such as cardiovascular illnesses, diabetes, cancer, and chronic lung diseases cause 60% of global deaths; of which, 80% occur in developing countries. In established health systems, primary healthcare handles most of the NCD care. This is a mixed-method study conducted to analyze the health service availability and readiness toward NCDs using the SARA tool. It included 25 basic health units (BHUs) of Punjab, which were selected through random sampling. Quantitative data were collected using the SARA tools, while qualitative data were collected through in-depth interviews with healthcare providers working at the BHUs. There was a problem of load shedding of both electricity and water in 52% of the BHUs, which leads to the poor availability of healthcare services. Only eight (32%) out of 25 BHUs provide the diagnosis or management of NCDs. The service availability was the highest for diabetes mellitus (72%), followed by cardiovascular disease (52%) and then chronic respiratory disease (40%). No services were available for cancer at the BHU level. This study raises issues and questions about the primary healthcare system in Punjab in two areas: first, the overall performance system, and second, the readiness of basic healthcare institutions to treat NCDs. The data show that there are many persisting primary healthcare (PHC) deficiencies. The study found a major training and resource deficit (guidelines and promotional materials). Therefore, it is important to include NCD prevention and control training in district training activities. NCDs are underrecognized in primary healthcare (PHC).

Prevalence and diversity of ectoparasites in Wild Rock Pigeon (Columba livia) in Punjab region, Pakistan.

The current study was carried out to estimate the prevalence and diversity of ectoparasites in rock pigeons in different regions of Punjab, Pakistan. A total of 120 birds were captured from March 2017 to February 2019. The ectoparasites were collected by standard procedures and preserved in 70% ethanol containing one drop of glycerin. Data related to age, health status, sex, type of area, sampling location and season were collected using a standardized form. Ectoparasites were identified based on morphological characteristics by using identification keys. Ninety-six (80%) birds were infested with ectoparasites. A total of seven families and thirteen species of different ectoparasites were observed. Mainly, seven species of lice, two species of flies, one species of tick and three species of mites were recovered from infested birds. The female pigeons were more often infested (89.02%) than male pigeons (60.52%). The prevalence was found higher during summer (100%) as compared to other seasons. The infestation rate was higher in Industrial area (97.50%) as compared to other regions. The highest prevalence of ectoparasites (100%) was recorded from Sargodha district. There was significant (P < 0.05) variation among number of ectoparasites on wing, chest, tail and neck within age groups, seasons and ecological zones. The occurrence of parasites in relation to area, age, health status, sex and season were significant. The infestation rate of parasites in rock pigeon is high in different districts of Punjab. It is recommended that these wild birds infested with multiple species of ectoparasites could be the potential source of infestations in domesticated birds if they come in contact with them. The contact of domesticated birds should be prevented from wild birds to minimize the chance of cross species transmission of ectoparasites.

Detection of antimicrobial resistance genes in extended spectrum beta-lactamase-producing Escherichia coli from milk of indigenous Beetal goats of Punjab.

Antimicrobial resistance (AMR) is a burning issue in the present era. Mastitis in dairy animals is one of the most important causes of huge production loss to dairy farmers. Aims: The study aims to find the prevalence, antimicrobial resistance profile, and resistance genes in the extended-spectrum beta-lactamase-producing Escherichia coli in mastitic milk. A total of 125 milk samples were collected from Beetal goats suffering from clinical mastitis from different districts of Punjab and processed for bacterial isolation and further identification. The drug resistance profile of ESBL-producing E. coli and its associations with molecular markers was analyzed using statistical analysis. The prevalence of ESBL-producing E. coli in dairy goats of Punjab was recorded as 6.4%. The isolates showed the highest resistance to the beta-lactam group of antibiotics. The resistance percentages of streptomycin, gentamicin, tetracycline, chloramphenicol, clotrimazole, and colistin were 50%, 37.5%, 50%, 25%, 25%, and 50%, respectively. The isolates showed intermediate resistance to imipenem (12.5%) and tetracycline (25%). The ESBL-producing E. coli isolates harbored the resistance genes blaCTXM (100%), blaTEM (62.5%), blaSHV (25%), blaOXA (37.5%), tetA (37.5%), tetB (25%), aadA (37.5%), sul1 (25%), MOXM (12.5%), DHAM (25%), and blaCMY-2 (50%). Tetracycline and sulphonamide resistances were statistically associated with their respective resistance genes (P<0.05). Streptomycin resistance was not statistically associated with the presence of the aadA gene (P>0.05). The genes blaIMP and blaNDM were not recorded in any of the isolates. In this study, 12.5% of the isolates showed co-resistance to colistin and carbapenem. Antimicrobial resistance is a hot topic and requires immediate attention.

Critical review of School Health &amp; Nutrition Program for achieving Advance Preventive Healthcare for Children in Punjab

The School Health and Nutrition (SHN) program was launched in year 2007-08 in different districts of Punjab, Pakistan. As the core of this program, School Health and Nutrition Supervisors (SHNS) were hired as the main agents to implement the program’s objectives. These SHNS were appointed at Basic Health Units (BHUs) and tasked with the objectives to screen the children in public schools, identify sick children, ensure their medical treatment, referrals, and follow-ups along with conducting health education sessions in their respective schools and communities. The current paper attempted to assess the performance of this program and suggested succinct measures to reform it in order to achieve its desired objectives. It included the analysis of the reasons and bottlenecks due to which the highlighted anticipated outcomes of this program could not be fully realized. After analyzing the qualitative and quantitative shortcomings in the design and implementation of this program, the current article recommends workable solutions based on strategic revisions in both the clinical as well as the procedural part of the program.&#x0D; Keywords: adolescent health, preventive healthcare system, rural health, rural nutrition program, school children&#x0D;

Techno-economic feasibility analyses of grid- connected solar photovoltaic power plants for small scale industries of Punjab, Pakistan

The globally soaring energy prices and electricity shortfall are major hurdles in the economic development of Pakistan. To cope with periodic power outages, small and medium enterprise (SME) business owners have to fall back on alternate power sources such as backup generators and uninterruptible power supplies (UPS), which further increase the per kWh cost of electricity, power quality issues, and greenhouse gas (GHG) emissions. On the contrary, grid-tied solar photovoltaic (PV) systems are not only economical and sustainable but support the national power grid to mitigate environmental emissions. This study aims to investigate and compare the techno-economic viability of grid-connected solar photovoltaic power plants for the manufacturing SME sector in four different districts of Punjab, Pakistan. Based on the technical, financial, and environmental indicators, a detailed techno-economic, sensitivity, and GHG emission analysis is conducted using RETScreen Expert software. The research findings clearly show that the proposed solar PV projects for all four locations are technically, financially, and environmentally viable, however, Sargodha as compared to other sites is the most feasible location with the highest capacity factor of 17.8 %, highest internal rate of return 14.9 %, lowest payback period 7.7 years, and least levelized cost of electricity 8.5 ¢/kWh. For validation, the simulation results are compared with performance metrics from PV plants erected in various parts of the world. Applying the same research approach to the whole industrial sector of Punjab recommends adding 13,469 MW of PV capacity to satisfy the industry’s 20446.21 GWh annual energy consumption and to cut emissions by 90,17,581 t CO2 per year. This research work presents guidelines for researchers to evaluate the feasibility of suitable PV technologies for the SME sector thereby helping investors to have a holistic view of potential investment zones.

Molecular characterization of Xanthomonas citri subsp. citri isolates and its resistance sources in citrus germplasm

Bacterial canker caused by Xanthomonas citri subsp. citri is an important disease of citrus. Ten isolates were obtained from the 17 samples collected from different districts of Punjab. Pathogenicity test on Kinnow revealed that Xcc 6 isolated from Kagzi lime was the most aggressive isolate with per cent disease index of 50.28. Thirteen cultivars of citrus were screened with the Xcc 6 using the detached leaf method under laboratory conditions. Screening was also done under field conditions. The cultivar Limequat had shown a highly resistant reaction with no disease development under laboratory conditions as well as under field conditions. Similarly, mandarins except PAU Kinnow 1 which displayed moderately resistant reaction exhibited moderately susceptible response under field conditions. The least average size and number of lesions were exhibited by the cultivar PAU Kinnow 1 under laboratory conditions. The isolates displayed variable results for different biochemical tests. All the bacterial isolates were Gram negative, rod shaped and exhibited growth on nutrient agar medium. Antibiotic sensitivity test revealed chloramphenicol to be the most effective antibiotic. Molecular characterisation was conducted using 15 RAPD primers. Similarity coefficient ranging from 0.098 and 0.634 was obtained with the highest similarity between Xcc 3 from Ladhowal, Ludhiana and Xcc 4 from PAU, Ludhiana and minimum similarity between Xcc 6 from PAU, Ludhiana and Xcc 2 from Ferozepur with a similarity coefficient of 0.098. Dendrogram construction using Unweighted Pair Group Method with Arithmetic Mean analysis gave two separate clusters at 30 per cent similarity. Only Xcc 8 from Boparai Kalan, Ludhiana belonged to the second cluster while all the other nine fell in the first cluster.

Development of integrated rodent management module in pea crop

Survey of pea crop in different districts of Punjab revealed severe rodent damage to pea crop. Therefore, there is a need to develop integrated rodent pest management (IRPM) module in this crop. Three to six blocks (each of 0.4 ha) of pea crop were selected in Ludhiana, Fatehgarh Sahib and Sangrur districts for determining critical timing of rodent damage and to develop integrated rodent management technology for effective rodent management in pea crop. Rodent population was assessed before and after the treatments as well as during different stages of crop by burrow count method. Results revealed that rodents start making their burrows on bunds at early stage of crop and start migrating inside fields at pod formation stage. At this stage, single symptomatic burrow baiting with 0.005% bromadiolone or with 2% zinc phosphide bait along with timely weed management and physical elimination of rodents during irrigation prevented rodent damage.

Malaria vector sibling species distribution in different endemic districts of Punjab, India.

Malaria transmission in Punjab, India is mainly seasonal with variation in its endemicity that may be due to varying vector behaviour in different areas of the state, primarily attributed to the existence of sibling species complexes among the vector species. So far there is no report regarding the existence of malaria vectors sibling species in the state of Punjab, therefore, the present study was planned to investigate the status of sibling species of two main vectors of malaria viz. Anopheles culcifacies and Anopheles fluviatilis in different districts of Punjab. Mosquito collections were made through hand catch in the morning hours. Malaria vector species An. culicifacies and An. fluviatilis were morphologically identified and man hour density was calculated. Both the vector species were subjected to molecular assays for sibling species identification through amplification of D3 domain of 28S ribosomal DNA by allele-specific PCR. Four sibling species of An. culicifacies, were identified viz. A, B, C and E. Species A was identified from Bhatinda district, species B, C and E from. S.A.S. Nagar and species C from Hoshiarpur. Two sibling species S and T of An. fluviatilis were identified from districts S.A.S. Nagar and Rupnagar. Presence of four sibling species of An. culicifacies and two sibling species of An. fluviatilis in Punjab necessitates planning of longitudinal studies to ascertain their role in disease transmission so that appropriate interventions may be applied to achieve malaria elimination.

Iron Asseveration in Soil, Forage and Animals Blood and Feces Samples Collected from Different Districts of Punjab, Pakistan

Iron Asseveration in Soil, Forage and Animals Blood and Feces Samples Collected from Different Districts of Punjab, Pakistan

Squamous Cell Carcinoma and its Association to different chewing Habits in Punjab, Pakistan

Oral squamous cell carcinoma is the world’s most prevalent and alarming cancerous disorder. Primarily it was found that elderly men are mostly affected by this disease due to tobacco and excessive use of alcohol. However, some studies also show a higher incidence of OSCC among young patients less than 40 years of age. Objective: To assess the risk factors for oral squamous cell carcinoma in Lahore. Methods: A randomized retrospective study was performed at Mayo hospital, Lahore. 52 patients suffering from diagnosed oral squamous cell carcinoma were selected as sample from different districts of Punjab, Pakistan. Data was collected by using pre-tested questionnaire. Results: Among all patients 87.50 % patients were addicted to different habits i.e. smoking (23.07%) and some chewing habits such as Paan (8.65%), Sapari (2.88%), Naswar (16.34%) and some patients were found to be addicted to a combination of factors like (Smoking and Paan 2.88%), (Smoking and Naswar 18.26%), (Smoking and Sapari 3.84%), (Paan and naswar 4.80%) and (Paan and Supari 6.73%). The ages of patients were mostly above than 35 years; only 4 patients were below than 35 years. Conclusion: Present data and measures to gratify and slow down the progress of OSCC are scarce. Steps should be taken to create awareness regarding risk factors of OSCC, early diagnosis and how effective treatment can make a difference.

Practices of Examiners in Marking English Essay at HSSC Level

The present study was carried out to spot the raters’ practices in marking English essays at the intermediate level in contrast to the marking system of O-level English language assessment in Punjab, Pakistan. The preceding researchers opined that a significant number of students fail in English as a subject. To unravel, the facts a sample of 350 HSSC (Higher Secondary School Certificate) paper raters with mixed experience, academic and professional qualifications have been taken randomly from the different districts of Punjab. Many of them had worked as examiners or sub-examiner for more than 10 years. The researchers formulated, piloted, and self-administered a questionnaire by visiting their workplaces. SPSS has been used to assay the data, and results have been generated. The results deduced that HSSC paper raters never exercised any rubric. They deduct and award scores on their individual judgments about the kind/number of mistakes, length of an essay, and handwriting. There has been found a scarcity of inter-rater reliability. Further, they are self-trained and are ignorant of the objectives; set in the national curriculum for HSSC. The current study is significant as it has implications onboard officials, policymakers, and examiners, which will finally promote skill-based learning.

Maize yield projections under different climate change scenarios in different districts of Punjab

The study aimed to find out possible changes in climatic data (temperature and rainfall) from the regional climate model viz. PRECIS(Providing Regional Climates for Impact Studies)under different SRES scenarios (A1B, A2 and B2 scenario) by the mid (2021-2050) and end (2071-2100) century at six locations of Punjab representing different agroclimatic zones and to study their impact on maize yield using the crop growth simulation model.The results revealed that the different zones of the state are expected to bewarmer during the mid century and this trend has been projected to continue by the end of century because of increase in maximum and minimum temperature at all the locations.The CERES-Maize simulated significant decrease in duration and grain yield of maize crop under projected climate scenarios. The reduction in the crop duration and grain yield was found to be more under the A1B and A2 scenario (high emission scenario) followed by B2 scenario (low emission scenario)due to adverse effects on crop physiology.

Appraisal of iron accumulation in soil, forages, and blood plasma of sheep and goats: a case study in different districts of Punjab, Pakistan.

Minerals are essential for ruminants affecting significantly the production of grazing livestock. Iron level in forages, soil, and blood plasma of the small ruminants (goat and sheep) was investigated in three districts of Punjab. Atomic absorption spectrophotometer was used to determine the concentration of iron in collected samples. The results revealed that the mean Fe concentrations in soil of districts Sargodha, Mianwali, and Bhakhar were significantly varied and ranged from 21.85 to 23.78, 28.45 to 31.2, and 18.079 to 24.33 mg/kg, respectively. The Fe level in soil of Mianwali significantly varied and was higher than Sargodha and Bhakkar. The mean Fe concentration in forages which were used for feeding purpose were significantly varied and found between 10.95-14.49, 23.63-25.65, and 6.616-9.45 mg/kg for Sargodha, Mianwali, and Bhakhar, respectively. The mean Fe concentrations in blood plasma of goat which consumed the contaminated forages were 8.5026-11.763 mg/L in district Sargodha, 19.77-20.19 mg/L in Mianwali, and 5.508-5.858mg/L in Bhakkar. In blood plasma of sheep, the residual levels of Fe in districts Sargodha, Mianwali, and Bhakhar were ranged from 9.987 to 12.455, 15.8 to 19.785, and 3.425 to 6.383 mg/L, respectively. This study provides the data of metals effected by different sites and also their mobility from low to higher trophic level which enables us to study the iron toxicity in different trophic levels, and we recommend different safe limits and treatment in case of low and high metal profile.

Impact of Wastewater and Canal Water Irrigation on the Accumulation of Copper in Maize and Millet of Different Districts of Punjab Pakistan

In current work, we examined the absorption of metal Copper in generally grown food crops (Maize &amp; Millet), cultivated in the waste water irrigated different Districts of Punjab, Pakistan. Analyzed samples of Water, Soil, shoot and grain were processed through atomic absorption spectrometric method. Our findings of Copper in water and Shoot were highest for Millet of Sargodha, irrigated with wastewater (1.69 mg/kg and 1.43 mg/kg respectively). In soil and grain samples maximum absorption of Cu was obtained in the waste water irrigated Maize (Sargodha) and Millet (Sheikhupura). Overall, Cu level was within the acceptable parameters set by FAO/WHO (2001). In this study, we also find out different indices such as Enrichment factor, Translocation factor, Pollution Load Index, Bio-concentration factor, Daily intake of metal and Health Risk Index. The study determined that frequently intake of waste watered forages in experimenting zone may carry possible health uncertainties in inhabitants.

Age-dependent dose assessment of uranium intake from bottled water in Punjab state, India

Abstract Uranium, both a radioactive material and a heavy metal, poses a health risk due to its radiological properties and chemical toxicity. In the present study, uranium concentration and relative age-dependent effective dose have been measured in 27 commercial brands of bottled waters collected randomly from different districts of Punjab, India. Uranium concentration varied from 0.19 to 9.29 μg l−1 with a mean value of 1.58 μg l−1, a standard deviation of 1.95 μg l−1 and a median of 0.82 μg l−1. Uranium concentrations in all the samples were found to be lower than the World Health Organization (WHO) and U.S. Environmental Protection Agency (USEPA) prescribed limit of 30 μg l−1 and AERB India proposed a radiological based limit of 60 μg l−1. Based on permissible limits, all the samples were suitable for human consumption. The annual effective dose was determined by taking the recommended water intake values of different age groups. The mean annual effective dose for all age groups was well within the International Commission on Radiological Protection (ICRP) recommended reference dose level of 1 mSv y−1. The highest dose was calculated for infants, which makes them the most crucial group of the population.

Molecular detection of benzimidazole resistance in Haemonchus contortus of sheep in Punjab

Single nucleotide polymorphism (SNP) at codon 167, 198 and 200 of β-tubulin isotype 1 gene accounts for benzimidazole resistance in Haemonchus contortus. To assess the anthelmintic resistance to benzimidazole group, allele specific polymerase chain reaction (AS-PCR) was employed on 50 adults of local abattoir of Ludhiana and 138 harvested larvae of H. contortus from faecal samples collected from field flocks of 6 districts of western zone of Punjab. The adults were found to be homozygous resistant (rr) showing 100% prevalence of resistant allele (r). AS-PCR on 138 larvae collected from different districts of Punjab showed 49.28% (69) homozygous resistant (rr), 3.62% (5) homozygous susceptible (SS) and 46.37% (64) heterozygous (rS) population. Species was confirmed as H. contortus by PCR-RFLP from the three fragments of size 462 bp, 211 bp and 147 bp. The pioneer study on the molecular diagnosis of benzimidazole resistance in H. contortus showed an overall allele frequency for resistant and susceptible population to be 0.72 (TTC) and 0.28 (TAC), espectively indicating development of high level of resistance in sheep.