Different Concentrations Of Ethylene Glycol Research Articles

Effect of ethylene glycol on micellization and micellar-catalyzed alkaline hydrolysis reaction of a cationic surfactant at 293–313 K

Changes in critical micellar concentrations (CMC’s) of gemini surfactant, α, ω-ethane bis(dimethyl cetyl ammonium bromide) (C16-2-C16) with different concentrations of ethylene glycol (EG) addition have been investigated by electrical conductivity method. Subsequently, alkaline hydrolysis of ethyl acetate (EA) in the presence of C16-2-C16 and C16-2-C16-EG has been studied conductometrically at 303.2 and 313.2 K, respectively. It was found that an increase in concentrations of EG added to C16-2-C16 aqueous solutions caused an increase in CMC’s of C16-2-C16, provoked by the decrease in the interfacial Gibbs energy contribution to GM∘. The hydrolysis of EA showed catalytic and restrained dual behavior in the presence of surfactant, it may be related to higher microviscosity and change of morphology with increased surfactant for C16-2-C16 at higher concentration. Addition of EG did not change microenvironment in micellar interfacial region significantly, which had less effect on gemini C16-2-C16 micellar catalytic efficiency.

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled Straw (OPS) method using different concentrations of ethylene glycol and dimethyl sulphoxide as cryoprotectants. After rehydration the oocytes underwent in vitro fertilisation; the resultant zygotes were then cultured in vitro for four days to assess embryonic development. In the first experiment, in vitro maturation of M and LW oocytes was compared. No significant difference was observed in the nuclear maturation rate of LW and M oocytes. In the second experiment, the sensitivity of oocytes to vitrification was examined by evaluating oocyte morphology after thawing. A higher percentage of LW oocytes showed normal morphology compared to M oocytes, indicating that Mangalica oocytes are more sensitive to cryoprotectants than Large White oocytes. After warming and in vitro fertilisation, more than 50% of the oocytes started embryonic development and by the end of the incubation period morula stage embryos had developed in both groups. The results show that the OPS vitrification technique is well suited to preserve Mangalica oocytes and from these oocytes morula embryos can be produced.

Optical fiber refractometer based on a Fabry-Pérot interferometer

A fiber optic sensor for liquid refractive index measurement based on a Fabry-Perot interferometer is described. The interferometer is achieved between the reflection of a short fiber Bragg grating and the Fresnel reflection from the cleaved fiber end. This fiber end is then in contact with the liquid sample to provide refractive index measurements. The sensor is characterized by immersing the fiber tip in distilled water with different concentrations of ethylene glycol. A linear relation of the interferometer fringe visibility with refractive index variation is observed, and a resolution of ~10−3 is obtained. It is also shown that the sensor operation is independent of temperature effects, other than the one related to temperature-induced change of the liquid refractive index.

70 EFFECT OF CRYOPROTECTANT CONCENTRATION ON THE IN VITRO SURVIVAL AND CELL PROLIFERATION OF PORCINE BLASTOCYSTS VITRIFIED USING THE OPEN PULLED STRAW SYSTEM

The objective of the present project was to study the effect of the concentrations of ethylene glycol (EG) and dimethyl sulfoxide (DMSO) during vitrification on the survival and hatching rates of porcine blastocysts. Embryos were collected by laparotomy from weaned crossbred sows (n = 18), vitrified, and warmed (one-step dilution) as described by Cuello et al. (2004 Theriogenology 62, 1144–1152). Vitrification was performed in different concentrations of EG and DMSO (15%, 16%, and 17% v/v for each cryoprotectant) or in an EG-based medium (40% v/v) using superfine open pulled straws. Fresh and vitrified blastocysts were cultured for 24 h in TCM199 and assessed by stereomicroscopy during the culture. Blastocysts that reformed their blastocoelic cavities after warming, displaying a normal zona pellucida and excellent appearance, were considered viable. The in vitro survival rate was defined as the ratio of viable embryos divided by the total number of embryos cultured. The hatching rate is determined as the ratio of the number of embryos hatched in vitro to the total number embryos cultured. Some vitrified and fresh embryos classified as viable were processed for Hoechst 33342 staining and proliferation cell nuclear antigen (PCNA) inmunolocalization. The proliferation index was defined as the number of PCNA-positive nuclei divided by the total number of nuclei stained with Hoechst 33342. Data were analyzed by ANOVA using the MIXED procedure (SPSS version 11.5; SPSS, Inc., Chicago, IL, USA). Data were expressed as mean values � SEM. The survival rate was similar for fresh and vitrified blastocysts, except for blastocysts vitrified using 15% cryoprotectants, which displayed a lower (P < 0.05) survival rate (84.2 � 4.8%) than fresh blastocysts (94.6 � 5%) and blastocysts vitrified using 40% EG, 16%, or 17% EG-DMSO (88.8 � 4.9, 96.8 � 4.9, and 96.4 � 5%, respectively). Fresh blastocysts showed a higher (P < 0.05) hatching rate (80.7 � 4.5%) than their vitrified counterparts (range: 48.4 � 7.7–55.3 � 7.8%). Vitrified and fresh blastocysts showed similar cell proliferation indexes (range: 75.8 � 3.2–83.7 � 3). When only hatched blastocysts among groups were compared, the proliferation rate decreased (P < 0.05) after vitrification with 17% EG-DMSO. Among vitrification groups, there was no significant difference in the number of total cells. However, vitrified blastocysts had a lower (P < 0.05) total cell number (range: 116.6 � 6.7–124.8 � 6.6) than fresh blastocysts (195.5 � 11.4). In conclusion, under our experimental conditions, the concentration of EG-DMSO can be decreased until 16% in the vitrification medium with no reduction of the in vitro developmental ability of the blastocysts. In addition, a 40% EG-based medium can be used for vitrification with results similar to those achieved using a medium containing 16% EG-DMSO.

Study of a rat model for calcium oxalate crystal formation without severe renal damage in selected conditions

Although nephrotoxic in high doses, ethylene glycol (EG) has been used with ammonium chloride (NH(4)Cl) or vitamin D(3) to study calcium oxalate stone formation in rat models. In the present study we used EG alone or with NH(4)Cl to study hyperoxaluria, crystaluria, and crystal attachment to renal epithelial cells in rats with minimal renal damage. Six-week-old male Sprague-Dawley (SD) rats were given food and special drinking water. In experiment 1 the drinking water contained 1.0% NH(4)Cl plus four different concentrations of EG (0.8%, 0.4%, 0.2%, 0.1%). In experiment 2 the drinking water contained EG alone (0.8%, 0.4%, 0.2%, 0.1%). Urine was collected for 24 h before the rats were sacrificed. In experiment 1 the rats were sacrificed 5-13 days after starting the special water. In experiment 2 the rats were sacrificed 7-21 days after starting the special water. Bladder urine was also obtained. Blood and urine were tested for calcium, phosphorus, and creatinine. In addition, urine was tested for pH, oxalate and N-acetyl-beta-D glucosaminidase (NAG). Kidney sections were stained with hematoxylin-eosin, von Kossa and Pizzolato stain. Crystal morphology was determined using polarizing microscopy, and composition was determined using high-resolution X-ray powder diffraction. Experiment 1: Aggravation of renal function, an increase in urinary oxalate and NAG excretion, and crystals observed in the kidneys all correlated with EG concentration and length of drinking time. In bladder urine, calcium oxalate monohydrate (COM) crystals exceeded calcium oxalate dihydrate (COD) crystals. Experiment 2: Renal function remained unchanged. Oxalate excretion increased and NAG increased slightly. Crystals occurred only in the papillary tip region. Crystals in bladder urine were mostly COD. In the current rat model, calcium oxalate crystaluria could be induced without severe renal damage in selected cases. Either and/or both COM and COD might form and interact with kidney epithelium. We propose different experimental conditions to study the various phases of calcium oxalate stone formation in young male SD rats.

Dielectric Studies of Glycine–Ethylene Glycol–Water Solutions Using Time Domain Reflectometry

Time domain reflectometry (TDR) has been used for dielectric relaxation measurements on the glycine–ethylene glycol–water ternary system (TDR) at 25, 30, 35, and 40°C in the frequency range from 10 MHz to 10 GHz. Glycine–ethylene glycol–water solutions are prepared with different concentrations of ethylene glycol (0, 5, 10, 15, 20, and 30%) and also for different glycine molar concentrations (0, 0.2, 0.4, 0.6, 0.8, and 1 M). The dielectric relaxation parameters are measured for aqueous glycine solutions also to compare the results with those for the glycine–ethylene glycol–water ternary system. For all the solutions considered, only one relaxation peak was observed in this frequency range. The complex permittivity spectra for the aqueous glycine solutions can be well described by the Cole–Davidson expression, whereas that for the ternary system can be well described by the Havriliak–Negami expression. The logarithm of the relaxation time log(τ) shows a nonlinear relation with the glycine molar concentration that implies a change in the relaxation mechanism with glycine concentration. The dielectric strength Δe increases with an increase in glycine molar concentration, whereas it decreases with an increase in ethylene glycol concentration.

Isolated ovine primordial follicles cryopreserved in different concentrations of ethylene glycol.

Cryopreservation of primordial follicles represents an opportunity to preserve female gametes, and consequently to protect the reproductive capacity of humans and animals, as well as to safeguard genetic material from endangered animal species or rare breeds. The aim of this work was to assess the toxicity of different concentrations of ethylene glycol (EG) to primordial follicles, and verify the viability of these follicles after the freezing-thawing procedure. Primordial follicles were isolated from ovine ovaries and exposed to different EG concentrations to evaluate the cryoprotectant (CPA) toxicity before and after cryopreservation. After isolation of primordial follicle (control), the number (mean+/-S.E.M.) of viable primordial follicles/ml was 3764+/-795.21. The number of viable follicles in the toxicity test using EG at 0, 0.5, 1.0, 1.5, 2.0, and 2.5 M was 1096+/-447.9, 960+/-446.67, 948+/-366.14, 832+/-313.59, 856+/-280.67, and 700+/-255.02, respectively. The number of viable follicles at concentrations of 2.5 M was less than for controls. After cryopreservation, the numbers decreased to 0+/-0, 148+/-85.46, 764+/-246.69, 824+/-291.9, 844+/-296.27, and 588+/-200.65, respectively for 0, 0.5, 1.0, 1.5, 2.0, and 2.5 M EG. The number of viable follicles at 0, 0.5, and 2.5 M was less than for controls. In conclusion, after the freezing and thawing procedure, concentrations of 1.0, 1.5, and 2.0 M EG can be successfully used for the cryopreservation of isolated follicles in sheep.

Thermal Stability of Collagen Fibers in Ethylene Glycol

The mechanism that renders collagen molecules more stable when precipitated as fibers than the same molecules in solution is controversial. According to the polymer-melting mechanism the presence of a solvent depresses the melting point of the polymer due to a thermodynamic mechanism resembling the depression of the freezing point of a solvent due to the presence of a solute. On the other hand, according to the polymer-in-a-box mechanism, the change in configurational entropy of the collagen molecule on denaturation is reduced by its confinement by surrounding molecules in the fiber. Both mechanisms predict an approximately linear increase in the reciprocal of the denaturation temperature with the volume fraction ( ϵ) of solvent, but the polymer-melting mechanism predicts that the slope is inversely proportional to the molecular mass of the solvent ( M), whereas the polymer-in-a-box mechanism predicts a slope that is independent of M. Differential scanning calorimetry was used to measure the denaturation temperature of collagen in different concentrations of ethylene glycol ( M = 62) and the slope found to be (7.29 ± 0.37) × 10 −4 K −1, compared with (7.31 ± 0.42) × 10 −4 K −1 for water ( M = 18). This behavior was consistent with the polymer-in-a-box mechanism but conflicts with the polymer-melting mechanism. Calorimetry showed that the enthalpy of denaturation of collagen fibers in ethylene glycol was high, varied only slowly within the glycol volume fraction range 0.2 to 1, and fell rapidly at low ϵ. That this was caused by the disruption of a network of hydrogen-bonded glycol molecules surrounding the collagen is the most likely explanation.

The Beneficial Effect of Organic Solvents on the Enzymatic Synthesis of Nucleoside Analogues Using N-Deoxyribosyltransferases fromLactobacillus leichmannii(E.C. 2.4.2.6)

Addition of ethylene glycol (10% v/v) has a beneficial effect on the synthesis of 2′-deoxynucleosides catalysed by crude preparations of N-deoxyribosyltransferases from Lactobacillus leichmannii. In the absence of added organic solvent decomposition of products and starting materials by deamination or hydrolysis occurs giving rise to poor yields of products if the transfer reaction is slow. The glycosyl transfer reaction is unaffected by addition of organic solvent but decomposition of products and starting materials is largely suppressed. The organic solvent appears to inhibit selectively contaminating enzymes in the crude N-deoxyribosyltransferase preparation as the purified transferase does not possess hydrolytic or deaminating activity. Different concentrations of ethylene glycol and other organic solvents have been examined as inhibitors of the side reactions but 10% (v/v) appears to be the most effective. Using the N-deoxyribosyltransferase in the presence of ethylene glycol, a number of 2′-deoxynuc...

Effect of ethylene glycol on the phase transition kinetics of gluco- and galactocerebrosides

The effect of different concentrations of ethylene glycol in water on the phase transition (metastable → stable state) of Gaucher's glucocerebroside, of bovine brain cerebroside type II (non hydroxy acyl chains only) and of N-palmitoylgalactocerebroside has been investigated. The phase transition and its kinetics were inferred from the thermograms at different heating and cooling rates and confirmed by FTIR spectra of the cerebrosides in the different states. The significance of the conformational differences of the glucose and of the galactose residues with respect to their solvation, and the subsequent effect on the intermolecular interactions and the phase transition is discussed.