Different Concentrations Of Chitosan Research Articles

The Effect of Chitosan Type and Concentration on Physical Characteristics and Drug Release Behavior of Andrographolide-Chitosan Microparticles Prepared by Ionic Gelation – Freeze Drying

Andrographolide is low in solubility and bioavailability, with short half-life (t1/2 = 1.5-2 hours), with chitosan micro-particles expected to improve its dissolution. Therefore, the objective of this research was to investigate the effect of chitosan concentration and the in vitro release of andrographolide from micro-particles prepared by ionic gelation using sodium tripolyphosphate (TPP) as cross-linker. Furthermore, the micro-particles used were prepared with two different concentration of chitosan namely 19 cPs and 50 cPs. The result showed that the micro-particles obtained were spherical in shape and heterogeneous in size, with Infrared spectra indicating ionic bonding between chitosan and TPP. The DTA thermogram and XRD diffractogram exhibited a change in crystal structure to a more amorphous form. The entrapment efficiency (EE) of andrographolide in micro-particles was about 78%, with no impact on the chitosan concentrations (19 cPs and 50 cPs) affected by EE. The kinetics released was followed by the Higuchi model in phosphate buffer, with pH 7 ± 0,05 higher than the andrographolide substance. It was investigated that andrographolide-chitosan micro-particles had the ability to enhance its dissolution rate.

Ecotoxicity evaluation: preparation of poly-ε-caprolactone and chitosan nanoparticles as carriers of thiamethoxam pesticide

The transmission of Huanglongbing (HLB) disease on citrus plants is through dissemination of the bacteria Candidatus Liberibacter ssp, by Diaphorina citri psyllid, its insect vector. Chemical control of the psyllids, and thiamethoxam (neonicotinoid insecticide) is one of the active ingredients used in the control of HLB. This insecticide is water soluble, unstable and rapidly degraded by photolysis. Pesticide nanoformulation is one of the strategies to control release of active compound as well as protection for premature degradation. Thus, studies of the effectiveness of encapsulated pesticide formulations are extremely important for enabling its use in agriculture. This study reports the encapsulation of the insecticide thiamethoxam in polymeric particles from poly-ε-caprolactone (PCL) and chitosan by double emulsion and solvent evaporation method using different concentrations of chitosan and two Pluronic (poloxamer) copolymers, F 127 and F68. These nanoparticles were characterized in terms of size, zeta potential, polydispersity, and encapsulation efficiency. The encapsulation efficiency, measured by liquid chromatography was 34%. The nanoparticles obtained from optimized conditions resulted in homogeneous and monodisperse particles with a positive superficial charge. The microalgae Raphidocelis subcapitata (bioindicator chloroficea) and microcrustacean Artemia salina, were used to evaluate the ecotoxicity of nanopesticide in comparison to pesticide already in the market. The ecotoxicity study demonstrated that nanopesticide was less toxic that commercial formulations in the studied conditions.

The rejection of mono- and di-valent ions from aquatic environment by MWNT/chitosan buckypaper composite membranes: Influences of chitosan concentrations

Owing to the scarcity of proper drinking water is an urgent problem, MWNT/Chitosan membrane is greeting to reject mono- and di-valent ions from water. MWNT/Chitosan membrane was fabricated through the dispersion of Multi-walled carbon nanotubes (MWNTs) in an aqueous solution containing different concentrations of chitosan. The influence of solution concentration on membrane salt rejection properties, as well as contact angle, electrical conductivity, water permeability, mechanical properties, zeta potential, surface area and internal pores morphologies has been investigated. The resulting buckypaper demonstrate that the contact angle (91° ± 4° to 124° ± 3°), electrical conductivity (17 ± 1 to 83 ± 3 S/cm), water permeability (0.59 ± 0.04 to 5.73 ± 0.3 L/m2 h bar), surface area and internal pores morphologies of the buckypaper membranes were decreased by increasing the concentration of chitosan. While, the mechanical properties (tensile strengths varied between 35 ± 2 and 75 ± 3 MPa) and zeta potential of these buckypaper membranes were found to increase with increasing the amounts of chitosan. A buckypaper fabricated from MWNTs and a high concentration of chitosan (0.4% w/v) showed a higher rejection efficiency for these salts, possibly due to their smaller internal pore volumes and lower specific surface area.

Chitosan‐based scaffold counteracts hypertrophic and fibrotic markers in chondrogenic differentiated mesenchymal stromal cells

Cartilage tissue engineering remains problematic because no systems are able to induce signals that contribute to native cartilage structure formation. Therefore, we tested the potentiality of gelatin-polyethylene glycol scaffolds containing three different concentrations of chitosan (CH; 0%, 8%, and 16%) on chondrogenic differentiation of human platelet lysate-expanded human bone marrow mesenchymal stromal cells (hBM-MSCs). Typical chondrogenic (SOX9, collagen type 2, and aggrecan), hypertrophic (collagen type 10), and fibrotic (collagen type 1) markers were evaluated at gene and protein level at Days 1, 28, and 48. We demonstrated that 16% CH scaffold had the highest percentage of relaxation with the fastest relaxation rate. In particular, 16% CH scaffold, combined with chondrogenic factor TGFβ3, was more efficient in inducing hBM-MSCs chondrogenic differentiation compared with 0% or 8% scaffolds. Collagen type 2, SOX9, and aggrecan showed the same expression in all scaffolds, whereas collagen types 10 and 1 markers were efficiently down-modulated only in 16% CH. We demonstrated that using human platelet lysate chronically during hBM-MSCs chondrogenic differentiation, the chondrogenic, hypertrophic, and fibrotic markers were significantly decreased. Our data demonstrate that only a high concentration of CH, combined with TGFβ3, creates an environment capable of guiding in vitro hBM-MSCs towards a phenotypically stable chondrogenesis.

Optimization and Physicochemical Characterization of Chitosan and Chitosan Nanoparticles Extracted from the Crayfish Procambarus clarkii Wastes

The main objective of this study was to allocate and evaluate the most optimum condition to prepare the chitosan and chitosan nanoparticles that will, subsequently, give the best physicochemical characterization regarding the nanoparticles' size and surface morphology. Chitosan from the exoskeleton wastes of Procambarus clarkii with degree of deacetylation 87% was extracted. Viscometry indicated that the obtained chitosan had a low molecular weight that can be useful in many applications. Chitosan nanoparticles were prepared by using different concentrations of chitosan and the polyanion sodium tripolyphosphate (TPP). For the determination of the optimum condition for the prepared chitosan nanoparticles, three different ratios of chitosan to the polyanion TPP were used, 1:1, 3:1, and 1:3. A novel nanoparticle system composed of low molecular weight chitosan was successfully prepared by the ionotropic gelation technique under an aqueous-based condition. The physicochemical characteristics of chitosan and chitosan nanoparticles were elucidated by Fourier-transform IR spectroscopy, nuclear magnetic resonance spectroscopy, elemental analysis, scanning electron microscopy, transmission electron microscopy, and zeta potential. The best physicochemical characteristics were obtained by adding chitosan to TPP in a ratio of 1:1. The small particle size and narrow range distribution of the obtained chitosan nanoparticles may increase their chance for easy and efficient manipulation for several applications in various fields. In addition, the obtained positively charged chitosan nanoparticles can be used for the delivery of negatively charged compounds.

Observation on the drag reducing effect of low concentration chitosan solution in turbulent pipe flow

Polymer-based drag reducing agent (DRA) is used widely in various industries due to its ability to enhance fluid flow as well as economically beneficial to various sectors. This study is conducted to observe the drag reducing effect of chitosan as DRA and observed its effect on the turbulent flow inside the pipe. The experiment is conducted in a closed loop circulation system where water is the transport medium. The pipe system consist of acrylic pipes with 0.013 m, 0.025 m and 0.038 m diameter. The chitosan was extracted from shrimp shells and five different concentration of chitosan were tested. The degree of deacetylation of the chitosan shows 97% which indicates its solubility in water. It was found that the highest drag reduction percentage were obtained from 0.038 m pipe of 33 ppm concentration which is 28%. The flow pattern inside the pipe is also analysed and discussed in this study

The Elasticity of Calcium Phosphate MAO Coatings Containing Different Concentrations of Chitosan

Calcium-phosphate coatings were formed on the commercially pure titanium by the method of microarc oxidation (MAO) from the electrolyte, which additionally contains 2.5-15wt.% solution of chitosan. The surface morphology, wettability and elasticity of the formed coatings were investigated. It is shown that the addition of chitosan to the electrolyte composition increases porosity and elasticity of the coatings.

Utilization of chitosan/Ag bionanocomposites as eco-friendly photocatalytic reactor for Bactericidal effect and heavy metals removal

Chitosan is a nontoxic, eco-friendly, and biocompatible natural polymer which could be used in an extensive range of applications for example in the areas of membranes, biomedicine, hydrogels, wastewater treatment, food packaging. Moreover, chitosan based nanomaterials had high sorption capacities, chelating activities, stability, and versatility, that would be potentially applied as green reactants in various scientific and engineering applications. The current study involved the preparation of silver nanoparticles incorporated into chitosan thin films and used for various purposes including photo-oxidation of organic pollutants, heavy metal removal (Cd, Pb, Cr, and Fe) and antibacterial activity. The fabricated chitosan/silver (CS/Ag) bionanocomposites thin films were characterized by the ultraviolet-visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and Fourier transforms infrared (FT-IR) spectroscopy. Furthermore, the prepared CS/Ag bionanocomposites had revealed good photodegradation rate, heavy metals removal and antimicrobial activity against gram-negative bacteria like E. coli, and gram-positive bacteria like G. bacillus, with increasing the loading of different concentrations of chitosan and silver nanoparticles incorporated into the prepared bionanocomposite thin films. Consequently, the prepared CS/Ag bionanocomposites are considered good candidates for wastewater treatment through photo-oxidation of organic pollutants, heavy metal removal as well as respectable antibacterial materials.

Chitosan hydrogel inclusion in fish mince based emulsion sausages: Effect of gel interaction on functional and physicochemical qualities

Chitosan has been reported to possess diverse applications in food industry. The cross linking properties of chitosan beneficially affects the protein-carbohydrate interactions which in turn enhances the functional properties in emulsion based meat products. In this context, the current study was conducted to incorporate hydrogel with different concentrations of chitosan (0%, 0.125%, 0.25%, 0.375% and 0.5%) into fish mince (Pangasianodon hypophthalmus) sausages in order to develop a functional sausage products with appealing flavour and desirable texture. The comparative analysis of various functional properties of the produced sausages showed that inclusion of chitosan gel had significant effect (p < 0.05) on the emulsion stability, gel-strength, pH, cooking yield, WHC, structural-compactness, texture, colour and sensory attributes of the products when compared with control. Further, from the study, it can be inferred that, sausages with 0.25% chitosan content (CS-2) uphold the best necessary functional and physiochemical attributes and presumed to be a superior ready-to eat product.

In Vitro Evaluation of Chitosan Induced Cytotoxicity against Cancerous Cell lines: MCF-7, Saos-2 and HeLa

Objective/ Background: Cancer is still the most common cause of morbidity in world and new powerful anticancer agents without severe side effects from natural sources is important. Methods: The evaluation of cytotoxicity and apoptosis induction was carried out in MCF-7,HeLa and Saos-2 as cancerous cell lines with different histological origin and human fibroblast served as control normal cell. The cells were treated with different concentrations of chitosan and the cytotoxicity was determined using MTT assay after 24, 48 and 72 h .The mode of death was evaluated by flow cytometry . Results: While both types of chitosan showed significant concentration-dependently cytotoxic effects against the three cancerous cell lines, fibroblast cells showed somehow more compatibility with chitosan. On the other hand, there were no significant differences between LMWC and HMWC cytotoxicity in all cell lines. The flow cytometry results showed the apoptosis pattern of death more in Saos-2 and HeLa while necrosis was more observable with MCF7. Also higher viability with both types of chitosan was seen in fibroblast as normal cells Conclusion: Chitosan shows anticancerous effect against 3 cancerous cell lines, while it is compatible with normal diploid fibroblast cells. Furthermore, it seems that the molecular weight of chitosan does not affect its anticancerous property.

Morphology dependent electrical property of chitosan film and modeling by fractal theory

The present study proposes a more general fractal approach-iterated function system (IFS) model for explaining the morphology of a biopolymer like chitosan dissolved in various organic acids and forming film and compared with the experimental results through multifractal approach. The bio polymer like chitosan has been dissolved in various organic acids like formic, acetic, adipic and the film of the same also have been formed through solution casting technique. The morphology of the prepared films have been obtained from scanning electron microscope (SEM) and the electrical impedance from LCR HIOKI Hi-Tester. The IFS theory is used to generate the morphology of chitosan film dissolved in various organic acids of various concentrations. In order to draw the similarity between morphological structure of experimentally obtained self-similar property of chitosan dissolved in various acids of different concentration of chitosan and the same has been generated by random iterated algorithm. The generalized fractal dimensions are estimated and compared with the electrical property like impedance which follows the same trend. Further, the growth of bacterial colonies and its self-similar structure has been generated by the same method. Hence it is inferred that our model is a more general model for the generation of self-similar property exhibited by nature.

Changes in the quality of sliced mortadella sausages prepared using chitosan

AbstractThis study aimed to evaluate the changes in the quality of mortadella sausages with different concentrations of chitosan during the cold storage period. The mortadella was prepared using 0, 1, 2, and 3% chitosan. It was then sliced, vacuum packed and stored at 4°C for a period of 60 days. During the storage period pH, water activity, oxy‐reduction potential, residual nitrite, and degree of oxidation were evaluated. In addition, the development of coagulase‐positive staphylococci, Salmonella spp., Clostridium reducing sulfite, coliforms, psychrotrophic microorganisms, mold, and yeast as well as lactic acid bacteria were analyzed. Sensorial analyzes to characterize mortadella were performed using the check‐all‐that‐apply (CATA) method. Mortadella prepared with chitosan presented technological feasibility, an antimicrobial and antioxidant activity, sensory acceptance, and in addition it is a source of fibers.Practical ApplicationsMortadella is a meat product consumed in many countries, however, it is food that needs to improve its image for the consumer, mainly because it is rich in fat, salt, and synthetic additives. Chitosan is a natural polysaccharide that has been studied as a functional ingredient in foods, possessing antioxidant, and antimicrobial characteristics. The addition of chitosan to mortadella can improve the quality of the product and provide the consumer a meat product with nutritional benefits and greater stability and shelf life.

Treatment of breast cancer with engineered novel pH-sensitive triaryl-(Z)-olefin niosomes containing hydrogel: an in vitro and in vivo study

Triaryl-(Z)-olefin (TZO) was synthesized as a Tamoxifen (TMX) analogue for breast cancer treatment to avoid developing the resistance and toxicity of TMX. TZO was synthesized using McMurry olefination reaction and has anti-cancer activity better than TMX by two folds. In this paper, in situ pH-sensitive TZO-loaded noisome hydrogel was prepared for delivering and targeting TZO to its site of activity. Equi-molar of cholesterol and span 60 was used to prepare TZO-loaded niosomes using the Hand Shaking Method. The central composite experimental design was used to prepare differently in situ pH-sensitive TZO-loaded niosomes formulae. The formulae were done by incorporated TZO-loaded niosomes into different concentrations of chitosan and Glyceryl monooleate (GCM). Increasing the chitosan and GCM concentrations resulted in significantly increasing the viscosity and significantly decreasing the release of TZO from different formulae. The formula composed of (0.61% w/v) of chitosan and (0.23% w/v) of GCM was chosen as an optimum formula to evaluate the efficacy of TZO using Ehrlich carcinoma mice model. A significant anti-tumour effect was shown in comparison with TMX. Briefly, in situ pH-sensitive TZO-loaded niosomes could be an effective treatment for breast cancer.

Kinetic models of swelling and thermal stability of silver/poly(vinyl alcohol)/chitosan/graphene hydrogels

Silver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by UV–vis spectroscopy (UV–vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM) and Fourier-transform infrared spectroscopy (FT-IR), thermal characteristics were determined by differential scanning calorimetry (DSC) and mechanical properties were measured by tensile test. The swelling studies were carried out in phosphate buffer to simulate natural physiological environment and data were fitted by several kinetic models to determine the diffusion mechanism and diffusion coefficients of the swelling medium through the hydrogel matrices. It was shown that the presence of silver nanoparticles increased the uptake capability and equilibrium swelling degree of the composite hydrogels. The antibacterial activity was confirmed against Escherichia coli and Staphylococcus aureus, while the hydrogels without AgNPs exhibited antibacterial properties due to the presence of chitosan. With the addition of AgNPs, the samples showed stronger activity and fast reduction in the number of colonies, confirming the synergistic effect of chitosan and AgNPs on the antibacterial activity.

Nanogel loaded with surfactant based nanovesicles for enhanced ocular delivery of acetazolamide.

Objective: Intraocular pressure has always been a great challenge for topical ophthalmic drugs. The study aimed to develop ocular surfactant based nanovesicles (NVs) carried in mucoadhesive nanogel providing efficient topical delivery of acetazolamide (ACZ).Methods: For the sake of optimizing formulation parameters, the effect of the type of edge activator and its ratio to sorbitan monostearate (Span 60) on the mean particle size, entrapment efficiency (%EE), and zeta potential (ZP) of produced NVs was investigated.Results: The selected formulation composed of Span 60:sodium deoxycholate with ratio 80:20 showed an average diameter of 202.90 nm, %EE of 90.2%, and ZP of −38.1 mV with a spherical and smooth surface. The ACZ loaded nanovesicles (ACZ-NVs) were embedded in different concentrations of Chitosan–sodium tripolyphosphate (CS-TPP) nanogels. The nanogel prepared using 1.5% CS showed the most promising viscosity, adhesion time, and rheological behavior (118,246 cP, 290 min, and thixotropic behavior, respectively). The in vitro release of ACZ showed a controlled release profile after incorporation in nanogels. The in vivo irritation test showed minimal irritation for the nanogel formulation compared to ACZ topical suspension. The effect of intraocular pressure lowering was significantly prolonged using ACZ-NV nanogels compared to ACZ oral tablets. Histopathological examination emphasized the healing power of CS on retinal atrophy.Conclusion: The research work indicated a promising potential for successful topical delivery of ACZ.

Co-encapsulation of imidacloprid and lambda-cyhalothrin using biocompatible nanocarriers: Characterization and application

A well-known strategy for managing pest resistance is application of mixture of pesticides. Conventionally formulated pesticides have several environmental incompatibilities. The use of biocompatible and biodegradable nanocarriers in formulating pesticides could improve environmental protection. In this study, a mixture of imidacloprid and lambda-cyhalothrin was co-encapsulated for the first time using liposomes as nanocarrier to simultaneously deliver these insecticides. Ethanol injection was used to produce self-assembled liposomes. The formed nanoliposomes were coated with different concentrations of chitosan. Nanoparticles were characterized by dynamic light scattering (DLS), scanning electron microscope (SEM) and FT-IR spectroscopy. The encapsulation efficiencies of lambda-cyhalothrin and imidacloprid were about 93% and 51%, respectively. The insecticide carrying liposomes had a size and surface charge of 57 nm and +0.6 mV, respectively. The size and surface charge of the particles produced were increased to 69 nm and +31 mV after being coated with chitosan (0.1%, W/V). In this study, residual activity of technical grade imidacloprid, lambda-cyhalothrin and their mixture and the effect of adjuvants used in commercial and nano formulations of these insecticides on Myzus persicae Sulzer was investigated. The insecticidal effects and duration of residual activity of nano-formulations was correlated with concentration of chitosan in final formulation. In accordance with the life cycle of M. persicae, using the mixture of imidacloprid and lambda-cyhalothrin improves the residual effect over their use alone. The use of lipid nanocarriers makes the improvement even further and can be a better alternative to conventional combination of these insecticides due to their more environmental friendliness.

Fabrication and characterization of chitosan film impregnated ciprofloxacin drug: A comparative study

Abstract Present study focuses on fabrication of biofilm from chitosan (natural polymer) for loading of the drug to heal the wound. The chitosan based film serves various advantages like biocompatibility, cost effective, easy formulation and sustained drug delivery for wound healing. Three different concentrations of chitosan (Ch) films were prepared like 1% (Ch1), 1.5% (Ch1.5) and 2% (Ch2). These films were impregnated with ciprofloxacin drug and they were characterized by various parameters like thickness, tensile strength, in-vitro drug release and swelling properties. The chemical structure and morphology of drug loaded biofilms were analyzed by fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) analysis and their antibacterial efficacy was also evaluated against bacteria such as Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The in-vitro biocompatibility was evaluated using NIH 3T3 fibroblast cell lines. The results revels that the ciprofloxacin loaded chitosan biofilm might serve as a potential drug delivery system for wound healing applications.

Optimization of chitosan nanoparticles as an anti-HIV siRNA delivery vehicle.

Chitosan has emerged as a promising polysaccharide for gene/siRNA delivery. However, additional works will be required to modify chitosan nanoparticles. In the present study, chitosan nanoparticles were well modified to introduce anti-HIV siRNA into two mammalian cell lines, macrophage RAW 264.7 and HEK293. We first generated two stable cell lines expressing HIV-1 Tat, and then designed and generated an efficient anti-tat siRNA. The nanoparticles were prepared by using different concentrations of chitosan, polyethylenimine (PEI) and carboxymethyl dextran (CMD) in various formulations and then their physicochemical and biological properties were investigated. The results demonstrated that the combination of chitosan with both CMD and PEI significantly improved both cell viability and siRNA delivery. The modified chitosan nanoparticles (ChNPs) at the N:P ratio of 50 were approximately uniform spheres with sizes ranging from 100 to 150 nm and a positive zeta potential of about +22 mV. In both cell types, the nanoparticles noticeably increased siRNA delivery efficiency with no significant cytotoxicity or apoptosis-inducing effects compared to the control cells. In addition, the nanoparticles significantly reduced the RNA and protein expression of HIV-1 tat in both stable cells. These data show that the nanoparticle formulation could potentially be used in gene therapy, especially against HIV infection.

Diosgenin Biosynthesis in the Sprouts of Fenugreek as Influenced by Chitosan

Trigonella foenum-graecum L. (Fabaceae), known as Fenugreekis an annual herb, contains various secondary metabolites including steroidal saponins, flavonoids, phenolic compounds and alkaloids. This study was planned to investigate the effect of concentrations of chitosan on growth, expression of diosgenin biosynthetic genes and diosgenin accumulation in sprouts of T. foenum-graecum. Results revealed that there is no positive response of chitosan in any of the studied growth parameters i.e. shoot and root length, fresh and dry weight. In control treatment without any chitosan solution the highest values for all the parameters were observed. The results indicated that increasing concentrations of chitosan had no significant effect on shoot and root lengths. The fresh weight of sprouts treated with chitosan decreased markedly, but not significantly, compared with that of the control. However, the dry weight of sprouts treated with chitosan was almost similar to that of the control. Diosgenin biosynthetic pathway in response to different concentration of chitosan enhanced to an increasing level in the expression of all treated sprouts than those of no chitosan (control) treated sprouts. The highest expression level was detected when treated with 0.05 mg/mL chitosan in most of the genes except TfSQS. The expression of TfSQS was slightly higher in sprouts treated with 0.01 mg/mL chitosan than in those treated with 0.05 mg/mL chitosan. The highest expression level was in TfCAS at 0.05 mg/mL chitosan exhibiting 3 times higher expressing than that of control. The increasing expression trend for both TfSQLE and TfSTRL remained up to 0.05 mg/mL chitosan showing 1.7 times more expression in this concentration and then started to decrease the expression level. Diosgenin content in the T. foenum-graecum sprouts did not respond positively to chitosan treatment. Our findings could be potentially implemented for future studies in this arena.

Effects of postharvest polyamine application and edible coating on maintaining quality of mango (Mangifera indica L.) cv. Langra during cold storage.

Mango is a tropical fruit which is sensitive to chilling injury. The present work investigated the potential of edible coatings of chitosan and polyamine spermidine in increasing shelf life and quality of mango. The control fruits (treated with distilled water) and the mango fruits treated with different concentrations of chitosan (0.5%, 1.0%, and 2.0%) and spermidine (0.5, 1.0, and 2.0 mM) were studied to improve postharvest characteristics and quality maintenance during cold storage. Parameters such as firmness, weight loss, fungal contamination, total phenol, antioxidant activity, vitamin C, pH, total soluble solids (TSS), titratable acidity (TA), flavor index, color index, and ethylene production were measured after at harvest (0), 8, 16, and 24 days of storage at 15 ± 2°C and relative humidity of 85%–90%. Chitosan and spermidine delayed water loss, firmness, and fungal contamination. Application of chitosan containing ascorbic acid significantly increased phenolic content and antioxidant activity compared to the control plants. It also changed soluble solid content, TA, pH of pulp, and sugar content and decreased ethylene production. The obtained results suggested that chitosan (2%) and spermidine (2 mM) had potential to improve firmness and delay deterioration processes of “Langra” mango after harvest.