Different Concentrations Of BAP Research Articles

In Vitro Shoot Multiplication and Rooting of 'Kashan' and 'Hervy Azerbaijan' Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications.

The damask rose (Rosa damascena Mill.) is an ornamental-medicinal plant from the Rosaceae family, and its aromatic compounds and essential oils are applied globally in the food, cosmetic, and pharmaceutical industries. Due to its economic value, this research aimed to establish a protocol for an efficient, rapid, and cost-effective method for in vitro shoot multiplication and rooting of the R. damascena 'Kashan' and 'Hervy Azerbaijan' genotypes. Nodal segments (as primary explants) were cultured on the Murashige and Skoog (MS) medium with combinations of various plant growth regulators (PGRs) such as gibberellic acid (GA3), 6-benzylaminopurine (BAP), and indole-3-butyric acid (IBA), as well as a PGR-like substance, phloroglucinol (PG), vitamins such as ascorbic acid (AA), and activated carbon in the form of active charcoal (AC). For the establishment stage, 0.1 mg·L-1 PG, 0.2 mg·L-1 GA3, and 1 mg·L-1 BAP were added to the media. Secondary explants (nodal segments containing axillary buds produced from primary explants) were obtained after 30 days of in vitro culture and transferred to the proliferation media supplemented with different concentrations of BAP (0, 0.5, 1, 1.5, 2, and 2.5 mg·L-1) and GA3 (0, 0.1, 0.2, 0.4, 0.8, and 1 mg·L-1) together with 0.1 mg·L-1 PG and 20 mg·L-1 of AA. The rooting media were augmented with different concentrations of BAP and GA3 with 0.1 mg·L-1 of IBA, PG and 20 mg·L-1 of AA and AC. The results showed that the highest regeneration coefficient (4.29 and 4.28) and the largest number of leaves (23.33-24.33) were obtained in the explants grown on the medium supplemented with 2 mg·L-1 BAP and 0.4 mg·L-1 GA3 for the 'Kashan' and 'Hervy Azerbaijan' genotypes, respectively. Likewise, this PGR combination provided the shortest time until bud break (approximately 6.5 days) and root emergence (approximately 10 days) in both genotypes. The highest number of shoots (4.78 per explant) and roots (3.96) was achieved in this medium in the 'Kashan' rose. Stem and root lengths, as well as stem and root fresh and dry weights, were also analyzed. In most measured traits, the lowest values were found in the PGRs-free control medium. Rooted plantlets were transferred to pots filled with perlite and peat moss in a 2:1 proportion and were acclimatized to ambient greenhouse conditions with a mean 90.12% survival rate. This research contributes significantly to our understanding of Damask rose propagation and has practical implications for the cosmetic and ornamental plant industries. By offering insights into the manipulation of regeneration processes, our study opens up new possibilities for the effective production of high-quality plant material.

Establishment of an Effective in vitro Regeneration System for Sugar Beet (Beta vulgaris L.)

Plant regeneration and dedifferentiation are known to be difficult for sugar beet (Beta vulgaris L.) due to its recalcitrant nature. This leads to low transformation efficiency and thus successful application of plant molecular techniques is limited in sugar beet for its genetic enhancement. A prolific regeneration method has been established by modulating several plant growth regulators on the in vitro regeneration of Beta vulgaris var. V6 KWS Serenada. Several types of explants excised from young seedlings of this variety were used for both direct and indirect regeneration of shoots. The highest response towards direct shoot formation and callus induction were obtained from cotyledonary nodes and hypocotyls, respectively. Explants were cultured on MS medium supplemented with different concentrations of BAP, GA3 and 2, 4 -D for callus induction as well as formation of shoots. Hypocotyls responded well for callus induction on MS medium containing 0.1 mg/l BAP + 2.0 mg/l 2, 4-D, while cotyledonary nodes exhibited the highest responses towards shoot formation on MS medium containing 1.0 mg/l BAP and 1.5 mg/l GA3. MS medium containing 2.0 mg/l IBA produced the highest number of roots per shoot. The in vitro raised rooted plantlets were successfully transferred to soil for acclimatization. Plant Tissue Cult. & Biotech. 33(2): 181-188, 2023 (December)

In vitro multiplication of Eugenia calycina Cambess. (Myrtaceae) under the influence of BAP and NAA

Eugenia calycina Cambess. (Myrtaceae) is a fruit species native to Brazil indicated for the recovery of degraded areas. Generally its propagation occurs by seeds, but leads to an increase in the juvenile period of the plants, problems in maintaining seed viability and pathogen incidence. However, with the application of tissue culture techniques it is possible to produce seedlings in large scale, uniformity of the plants obtained and reduction in the period of seed germination. Thus, the objective of this research was to define the best medium for in vitro multiplication of E. calycina. Nodal segments of 1 cm, extracted from E. calycina plants already established in vitro, were used as explants. The treatments consisted of different concentrations of BAP (0; 0.5; 1 and 2 mg L-1) and NAA (0; 0.5; 1 and 2 mg L-1). The experimental design was completely randomized, in a 4x4 factorial scheme, with four concentrations of BAP and four concentrations of NAA, with four replications and three tubes per replication. After 90 days, shoot number, length and fresh mass of the aerial part, production and fresh mass of callus were evaluated. The MS medium plus 2.0 mg L-1 of BAP is ideal for in vitro multiplication of E. calycina.

The influence of 6-Benzylaminopurine (BAP) on yield responses and photosynthetic physiological indices of soybean

Soybean plants produce a large number of flower buds. However, the majority of them do not become pods and die during development. The bulk of total reproductive abscission is caused by flower and pod drop, or abortion, which impacts prospective soybean yield. Plant growth regulators serve critical functions in plant development, but only recently have their tasks been recognized in the manifestation of yield components, product, and seed qualities in soybean. The objectives of this study were to see how different concentrations of 6-Benzylaminopurine (BAP) affected photosynthetic physiological indexes, as well as gene expression of chlorophyll synthesis at the transcript level in soybean plants. In the current investigation, spraying different concentrations of BAP had no significant effect on the soybean yield. However, the BAP generated significant alterations in chlorophyll content as compared to the control. Consequently, the use of BAP significantly boosted the net photosynthetic rate. Furthermore, the current study found that BAP significantly influenced stomatal conductance and net transpiration compared to the control plant. The application of BAP significantly improves the expression of all genes that encode for chlorophyll biosynthesis. The expression of genes in leaves exhibited more up-regulation as compared to the stem. In general, this work offers a new and better understanding of how BAP might be employed to boost yield and chlorophyll biosynthesis.

Propagation of Gaharu Plant Gyrinops Versteegii Species Provenant Beringin Throught in Vitro Culture

Grynops verstegii is a non-timber forest product commodity that has a very high economic value. People know this tree as the agarwood tree. In Lombok, the Beringin provenance is one of the leading provenances, so it is very important to conserve its germplasm. This study aims to find alternatives for gaharu development through seed production using in vitro culture techniques. This research was carried out in July 2021 at the Tissue Culture Unit of the Immunobiology Laboratory, University of Mataram. In this experiment there were 8 media treatments with different concentrations of BAP (Ssitokini) and NAA (auxin). The results showed that callus growth occurred on MS 1, MS 3, MS 4, MS 6, and MS 7 media. However, the best growth was obtained in MS 1 treatment with a combination of 1.0 ppm BAP and 0.5 ppm NAA. While most of the samples experienced browning and contamination.

In vitro Micropropagation of Berberis chitria (Lindl.) - A Rare Medicinal Plant from Himachal Pradesh, India

An efficient in-vitro micropropagation protocol has been developed for Berberis chitria (Lindl.) - a rare medicinal plant from Himachal Pradesh, India using seeds and in-vitro nodal explants. The effects of different concentrations of BAP and GA3 for in-vitro seed germination was investigated and consequently 6.0 mg/l BAP + 0.0 mg/l GA3 was reported to be excellent for seed germination. Callus induction from leaf explants from aseptically grown seedling were also studied by using various concentrations of BAP and 2, 4-D and showing 94.26 ± 0.12% response at the concentration of 2.0 mg/l BAP + 0.4 mg/l 2, 4-D. The highest percentage of multiple shoot induction was obtained (100.00 ± 0.01% with an average of 4.50 ± 0.3 shoots) on MS medium augmented with 6.0 mg/l BAP + 1.0 mg/l Kn + 1.0 mg/l IAA. Whereas, the combined concentration of 1.0 mg/l NAA + 1.0 mg/l IBA + 0.5 mg/l IAA showed highest (76.05 ± 0.09%) root induction with an average of 3.84 ± 0.27 roots per shoot. The survival rate of these plantlets under greenhouse condition was 85%. This research communication provides first record about the in-vitro multiplication of B. chitria, collected from Himachal Pradesh Himalayas of India. Plant Tissue Cult. & Biotech. 32(2): 227-236, 2022 (December)

In Vitro Propagation of Pyracantha angustifolia (Franch.) C.K. Schneid.

Narrow-leaf firethorn or pyracantha (Pyracantha angustifolia (Franch.) C.K. Schneid.), from the family Rosaceae, is a species of large and thorny evergreen shrub. In this study, a procedure is presented for efficient axillary shoot multiplication and root induction in P. angustifolia using Murashige and Skoog (MS), woody plant (WPM), and Linsmaier and Skoog (LS) culture media supplanted with 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA). The disinfection of the axillary buds was performed with a 70.23% success rate on a basal MS medium augmented with 0.5 mg·L−1 gibberellic acid (GA3). Uniform and axenic explants were then cultured on MS, WPM, and LS media enriched with different concentrations of BAP, 0.3 mg·L−1 GA3, and 0.1 mg·L−1 IBA. The highest multiplication coefficient (2.389) was obtained for the MS medium supplemented with 2.5 mg·L−1 BAP. After one month, newly formed micro-shoots were transferred to rooting media (MS, WPM, and LS) containing different concentrations of IBA, together with a constant concentration of 0.1 mg·L−1 BAP. The micro-shoots were kept in the dark for one week and then cultured in a 16/8 h light/dark regime. The MS medium supplemented with 1 mg·L−1 IBA was the most effective in stimulating rooting (88.76% of micro-shoots). The highest number of roots (3.5 per micro-shoot) was produced in the MS medium enriched with 1.5 mg·L−1 IBA. The rooted plantlets were transferred into pots filled with perlite and peat moss in a 2:1 proportion and acclimatized to ambient greenhouse conditions, with a resultant mean 92.84% survival rate. Thus, this protocol can be successfully applied for the in vitro mass propagation of P. angustifolia.

Effects of Sucrose and Myo-inositol on In Vitro Shoot Multiplicationof Promising Interspecific F1 Hybrid of Eucalyptus (Eucalyptus Tereticornis X Eucalyptus Grandis)

Abstract –Micropropagation technique is standardized for its multiplication, using nodal segments of 25-30 years old trees i.e. promising interspecific F1 hybrid of Eucalyptus (Eucalyptus tereticornis X Eucalyptus grandis). 0.1% Mercuric chloride solution for 10-15 minutes used for surface sterilization of nodal segments followed by 0.1% fungicide treatment for 1 minute and then washed 4-5 times with sterilized distilled water. These surface sterilized nodal segments were cultured on MS medium combination with auxin and cytokinin (NAA + BAP) for axillary bud proliferation. MS medium with combin- ation of 1.5mg/l BAP + 0.1mg/l NAA gave optimum rate of axillary bud induction. The in vitro shoot were cultured on MS medium with different concentration of BAP (0.1–3.0 mg/l) alone or in combination with NAA (0.1-1.5mg/l) and supplemented with sucrose at 3% level was the best for the growth and development of shoots. These proliferated axillary shoots were excised and subcultured on MS + 1.0 mg/l BAP + 0.1mg/l NAA medium to proliferate in vitro shoots.

In Vitro Plant Regeneration via Adventitious Shoot Regeneration from Cotyledon and Leaf Explants of Diplocyclos Palmatus

ABSTRACT Micropropagation protocol for Diplocyclos palmatus through direct shoot induction from isolated cotyledon and leaf explants was established. Cotyledons were isolated from 2-week-old seedlings and cultured in the Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP, 0.5–2.0 mg L−1) alone or in combination with α-naphthalene acetic acid (NAA, 0.2–1.0 mg L−1) for shoot induction. The highest frequency of response (89%) and the mean number of shots (6.8) were observed in an MS medium supplemented with 1.5 mg L−1 BAP and 0.5 mg L−1 NAA. Leaves at various stages of development were isolated from in vitro grown shoots and cultured in an MS medium supplemented with different concentrations of BAP (0.5–2.5 mg L−1), Thidiazuron (TDZ, 0.5–2.5 mg L−1) alone and in combination with NAA (0.2 mg L−1) for shoot induction. The highest shoot induction frequency of 91% and shoot number of 7.8 shoots per explant were observed when 15-d-old- leaves were cultured in an MS medium enriched with 2.0 mg L−1 TDZ and 0.5 mg L−1 NAA after 45 d. The isolated cotyledon and leaf-derived shoots showed optimum rooting on ½ MS medium fortified with 4.0 mg L−1 indole-3-butyric acid (IBA). The rooted plants were transferred to the soil with 90% success.

Stimulation of phenolic compounds accumulation and antioxidant activity in in vitro culture of Salvia tebesana Bunge in response to nano-TiO2 and methyl jasmonate elicitors

In the present study, we first attempted to achieve an efficient procedure for optimizing callogenesis from apical meristem and leaf explants of Salvia tebesana on MS media containing different concentrations of BAP alone and in combination with 2,4-D. Then, the inducing effect of nano-TiO2 (10, 60, and 120 mg L−1) and methyl jasmonate (50, 100, and 200 µM), as abiotic elicitors were studied on the enhancement of phenolic compounds, rosmarinic acid, and some individual flavonoids as well as antioxidant capacity of callus. According to the results, the highest callogenesis rate (100 and 93.33, respectively) and DW (0.55 ± 0.03 and 0.36 ± 0.02 g, respectively) per responsive explant were achieved from apical meristem on MS media containing "BAP1 + 2,4-D1" mg L−1 and from leaf explant on the medium supplemented with "BAP0.5 + 2,4-D1" mg L−1. The elicitation with 10 and 60 mg L−1 nano-TiO2 (respectively for apical meristem and leaf), and 50 µM MeJa could significantly promote the production of predominant phenolic derivatives in S. tebesana calli, where the highest content of total phenolics, O-diphenols, phenolic acid, flavonoid, flavone and flavonol, proanthocyanidin was recorded. As well as results declared that the increasing of rosmarinic acid was more correlated with nano-TiO2 treatment than MeJa elicitation. Moreover, the highest content of apigenin (0.33 ± 0.02 μg g−1 DW) was detected after MeJa-elicitation (50 µM), while the maximum level of quercetin (2.62 ± 0.09 μg g−1 DW) and rutin (13.79 ± 0.87 μg g−1 DW) were obtained after exposure to 60 mg L−1 nano-TiO2, both from leaf-derived calli. While a significant positive correlation was recorded between antioxidant assays (DPPH and FRAP) and phenolic derivatives of treated calli; a very strong correlation occurred between the content of rosmarinic acid of apical meristem-derived calli and DPPH and FRAP values (r2 = − 0.921 and r2 = − 0.913, P < 0.01 respectively). Our results showed that the combination of in vitro culture and elicitation would be a good technique to successfully produce and enhance the content of pharmacologically valuable metabolites in S. tebesana. Nano-TiO2 and methyl jasmonate elicitors in a concentration-dependent manner increased the phenolic compounds, specific flavonoids, and antioxidant properties in the callus culture of Salvia tebesana Bunge.

In-Vitro Regeneration of Interspecific F1 Hybrid (Eucalyptus citriodora and Eucalyptus torelliana) of Eucalyptus

Tissue culture technique was standardized for in vitro shoot multiplication, using nodal segments of 25- 30 years old trees i.e., promising interspecific F1 hybrid of Eucalyptus (Eucalyptus citriodora and Eucalyptus torelliana). Due to the phenolic exudation explants did not survive and eventually died without regenerating buds. The explants collected from July to September were the best for in vitro studies for icropropagation. The axillary buds were surface sterilized with 0.1% Mercuric chloride solution for 10-15 minutes, followed by 0.1% Bavistin treatment for 1 minute and subsequently washed 3-4 times with sterilized distilled water. These surface sterilized axillary buds were cultured on MS medium supplemented with cytokinin and auxin. MS medium supplemented with 1.5mg/l BAP + 0.1mg/l NAA proved to be the best hormonal combination for induction of axillary bud which resulted in the development of 1-3 axillary shoots. The proliferated shoots were cultured on MS medium with different concentration of BAP (0.1 – 3.0 mg/l) alone or in combination with NAA (0.1-1.5mg/l) and supplemented with sucrose at 3%. The aim of the paper was to obtain maximum rate of in vitro regeneration of FRI-15 by using micropropagation technique.

Effects of Taurine against benzo[α]pyrene-Induced Cell Cycle Arrest and Reactive Oxygen Species-Mediated Nuclear Factor-Kappa B Apoptosis via Reduction of Mitochondrial Stress in A549 Cells

Taurine is a free amino acid that prevents reactive oxygen species (ROS) formation. ROS production is associated with oxidative stress, cell proliferation, apoptosis, inflammation, and DNA alterations in benzo[α]pyrene (BaP)-induced lung cells. Here, we assessed the effect of adding of 25 mM taurine on human pulmonary alveolar epithelial A549 cells treated with different concentrations of BaP. After culturing for 24 h, the cells were tested for biomarkers including cell viability, cellular morphology, Annexin V-FITC/propidium iodide, cell cycle regulation, ROS accumulation, mitochondrial membrane potential (MMP), and expression of related signaling genes and proteins. BaP induced cell cycle arrest and decreased cell viability in a dose-dependent manner. In addition, 50 μM BaP induced a 52.2% increase in ROS levels and inhibited MMP by up to 80%; however, taurine decreased BaP-induced ROS production by 19.5% and restored MMP. The expression of nuclear factor-kappa B (NF-κB), B-cell lymphoma-2 (BCL-2) homologous antagonist killer (Bak), BCL-2-associated X protein (Bax), and cytochrome c at both the mRNA and protein levels were increased, and the expression of BCL-2 and BCL-x1 was decreased by BaP treatment. Furthermore, BaP activated caspase-3/7 expression by up to 25%. However, taurine decreased the expression of NF-κB, Bak, Bax and cytochrome c levels, reduced caspase-3/7 activities, and increased the expression of BCL-2 and BCL-x1. Hence, taurine attenuates BaP-induced oxidative stress and mitochondrial dysfunction by inhibiting the NF-κB-mediated intrinsic apoptosis pathway in A549 cells. Taurine can be considered as a preventive molecule to prevent lung damage.

Somatic Embryogenesis from Immature Olive Zygotic Embryos.

The effects of plant growth regulators (PGRs) and the explant type on the embryogenesis and plant regeneration of olive (Olea europaea L. ssp europaea var. sativa) cv. "Chetoui" were studied using immature zygotic embryos. Embryogenic callus induction was achieved on OMc medium supplemented with different concentrations of BAP and NAA at low levels. Immature zygotic embryos as juvenile tissues are competent for somatic embryogenesis independently of PGRs supplementation. Repetitive somatic embryogenesis was obtained on PGRs-free media or at low concentrations in the dark.

Effect of different concentrations of BAP and constant NAA on tissue-cultured regeneration of manga Bamboo (Dendrocalamus stocksii)

Effect of different concentrations of BAP and constant NAA on tissue-cultured regeneration of manga Bamboo (Dendrocalamus stocksii)

Tissue culture technique on micropropagation of grape B41

This research was carried out in the Tissue Culture Lab of the National Commission of Biotechnology (NCBT) in Damascus/ Syria and in Faculty of Agriculture– Damascus University during 2018- 2019 to optimize an effective method for micropropagation of grape Vitis vinifera L. Explants of (0.5-1cm) with adjacent bud were planted in multiplying stage on MS medium contains different concentrations of BAP (0, 0.5, 1, 1.5, 2, 3 mg/ l ), with or without 0.2 mg/ l NAA or IAA. The multiplied explants transferred in comparison with MS medium without hormones (control) with different concentrations of IAA (1, 2, 4, 8 mg/ l) for its rooting stage. The results revealed that the hormone combinations (2 mg/ l BAP + 0.2 mg/ l NAA) had the highest average of the plant length (3.233 cm), leaves and the number of formed buds (5.667 leaves, 4.667 bud/ plant biopsy respectively). The hormonal combinations BAP/ NAA led to an obvious increase in the studied parameters in comparison to hormonal combinations treatments BAP/ IAA and nutrition medium with BAP only treatments, whereas the hormonal combinations IAA/ NAA did not lead to an obvious increase in the number of leaves and formed buds in compare with nutrition medium with BAP only treatments. The best percentage of rooting (92.8%) was achieved in MS medium supplemented with (1 mg/ l IAA). 87% of the adapted produced plantlets from tissue culture were successfully moved and planted in soil.

Protocol Optimization for Rapid Multiplication of Cassava (Manihot esculenta Crantz) using the Nodal Segment

Background: Cassava is a vital food security crop for millions of people, especially in sub-Saharan Africa. Since the crop produces a reasonable yield on marginal soils, it could help alleviate world hunger. Consequently, the increase in cassava production and its quality characteristics are significant. However, the low multiplication rate of this main crop has delayed the spread of improved varieties among farmers. As a result, tissue culture techniques can be a viable solution to overcome these challenges. Methods: The study used a nodal segment as an explant to evaluate different concentrations of BAP and NAA for an efficient, cost-effective in vitro mass multiplication of the AWC-1 cassava variety. CuSO4, commercial bleach and ethanol had used to sterilize nodal explants taken from greenhouse-grown plants at various time intervals. Result: The best medium for micro shoots induction had found to be medium without growth regulators. Among different treatments used for shoot multiplication purposes, the maximum shoot number has been recorded on M.S. medium supplemented with 0.75 mg/l BAP and 0.2 mg/l NAA. Medium with 0.5 mg/l NAA concentration was the best for rooting induction. A survival rate of 86% has obtained in the greenhouse and the plantlets appeared to be morphologically normal.

Cryopreservation of grapevine shoot tips by frozen in vitro

This research was carried out in the Tissue Culture Lab of the National Commission of Biotechnology (NCBT) in Damascus/ Syria and in Faculty of Agriculture– Damascus University during 2018- 2019 to optimize an effective method for micropropagation of grape Vitis vinifera L. Explants of (0.5-1cm) with adjacent bud were planted in multiplying stage on MS medium contains different concentrations of BAP (0, 0.5, 1, 1.5, 2, 3 mg/ l ), with or without 0.2 mg/ l NAA or IAA. The multiplied explants transferred in comparison with MS medium without hormones (control) with different concentrations of IAA (1, 2, 4, 8 mg/ l) for its rooting stage. The results revealed that the hormone combinations (2 mg/ l BAP + 0.2 mg/ l NAA) had the highest average of the plant length (3.233 cm), leaves and the number of formed buds (5.667 leaves, 4.667 bud/ plant biopsy respectively). The hormonal combinations BAP/ NAA led to an obvious increase in the studied parameters in comparison to hormonal combinations treatments BAP/ IAA and nutrition medium with BAP only treatments, whereas the hormonal combinations IAA/ NAA did not lead to an obvious increase in the number of leaves and formed buds in compare with nutrition medium with BAP only treatments. The best percentage of rooting (92.8%) was achieved in MS medium supplemented with (1 mg/ l IAA). 87% of the adapted produced plantlets from tissue culture were successfully moved and planted in soil.

In vitro Plant Regeneration in Banana (Musa sp.) cv. Grand Nain

This study was conducted at the tissue culture laboratory in Pakistan. Suckers obtained from the banana cv. Grand Nain to explore the effects of different concentrations of BAP and IAA. After inoculation with Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) and the Indole-3- acetic acid (IAA) the suckers first turn into the brown after 5-10 days then turned into a green mass-like structure after 30 days. From this mass-like structure shoots and roots were developed. Among the different concentrations, BAP 7.5 mg/l and IAA 0.5 mg/l showed the highest number of shoots 0.75, 2.75 and 6.25 per explant and shows the highest shoot lengths which 1.03 cm, 2.45 cm and 3.40 cm respectively. IAA is essential for only roots proliferation. The roots of different lengths were produced when the concentration of IAA 0.5 mg/l was used and 2.93 cm, 4.63 cm and 5.88 cm roots length produced at 15, 30 and 45 days after occultation respectively. Then these plants were transferred to the greenhouse for hardening acclimatization with the environment, the hardening process took place, and the established plantlets are ready for planting.

The Effect of MS Media Strength and Cytokinin in the Induction of Shoots from Shoot Tip Explants of Australian Finger Lime (Citrus australasica cv. Tasty Green)

The Australian Finger Lime (Citrus australasica) is a type of citrus from the Rutaceae family, endemic to the east coast of Australia. The finger lime, loaded with numerous vitamins and renders a unique taste, has also been backed by science to contain essential amounts of antioxidants that are beneficial for cell protection, immune response, cancer prevention, ageing, arthritis and prevention of kidney stones. Current propagation attempts still rely on conventional methods that are less efficient and resulted in the slow establishment of farms for finger lime especially for commercialization purposes. This study focuses on the induction of shoots from shoot tip explants using 6-Benzylaminopurine (BAP) and Kinetin. Aseptic explants were inoculated into Murashige and Skoog (MS) medium of full-strength and half-strength followed by full-strength MS media supplemented with different concentrations of BAP and Kinetin. Results obtained in this study showed no significant differences in terms of the number of axillary shoots produced between explants cultured in full and half-strength MS media. However, the highest number of shoots and increment in shoot length were obtained from MS media supplemented with 2.0 mg/L BAP with the values 1.80 ± 0.27 and 2.56 ± 0.36 cm, respectively. In conclusion, MS media supplemented with 2.0 mg/L BAP was found optimal in the induction of shoots and shoot elongation of C. australasica cv. Tasty Green.

In Vitro Micropropagation of Lilium candidum Bulb by Application of Multiple Hormone Concentrations Using Plant Tissue Culture Technique

The multiplication efficacy by bulb is low and the plantlets are more susceptible to disease, therefore, there is a need to develop a protocol for its propagation. Lilium candidum is listed in the saitma prefecture Red Data Book as a critically endangered plant and rescuing information regarding its micro-propagation is rather limited. On this regard, the application of in vitro micropropagtion procedure might help to obtain large numbers of uniform plants of endangered species of Lilium. Dried lilies are a rich source of fiber and also rich in sodium and carbs. Lily bulbs have proteins and starch and also small quantities of iron, calcium, phosphorous, and vitamin B1, B2, C. The health benefits of the lily for the heart are well known on account of the active cardiac glycosides as well as the flavonoids which tend to stimulate the arteries and can cause them to dilute. Another one of the therapeutic uses of the lily flower is in the case of treating burns and preventing the formation of scar tissue. One of the main health benefits of the lily flower is that it helps regulating the heart rate there by allowing the heart to function more efficiently and regular. Having multiple medicinal properties we decided to cultivate Lilium candidum using plant tissue culture so farming can be increased using this cost efficient techniques. In this research, we have studied various Effect of different concentration of BAP and NAA on the initiation of Lilium candidum from bulb and IBA, IAA and NAA on the rooting of shoots of Lilium Candidum.