Effects of Sucrose and Myo-inositol on In Vitro Shoot Multiplicationof Promising Interspecific F1 Hybrid of Eucalyptus (Eucalyptus Tereticornis X Eucalyptus Grandis)

Abstract

Abstract –Micropropagation technique is standardized for its multiplication, using nodal segments of 25-30 years old trees i.e. promising interspecific F1 hybrid of Eucalyptus (Eucalyptus tereticornis X Eucalyptus grandis). 0.1% Mercuric chloride solution for 10-15 minutes used for surface sterilization of nodal segments followed by 0.1% fungicide treatment for 1 minute and then washed 4-5 times with sterilized distilled water. These surface sterilized nodal segments were cultured on MS medium combination with auxin and cytokinin (NAA + BAP) for axillary bud proliferation. MS medium with combin- ation of 1.5mg/l BAP + 0.1mg/l NAA gave optimum rate of axillary bud induction. The in vitro shoot were cultured on MS medium with different concentration of BAP (0.1–3.0 mg/l) alone or in combination with NAA (0.1-1.5mg/l) and supplemented with sucrose at 3% level was the best for the growth and development of shoots. These proliferated axillary shoots were excised and subcultured on MS + 1.0 mg/l BAP + 0.1mg/l NAA medium to proliferate in vitro shoots.