Different Concentrations Of Extract Research Articles

VAKUMLU EMDİRİM İLE RENKLİ FONKSİYONEL TAVUK ETİ KÜPLERİNİN ÜRETİLMESİ

Bu çalışmada; vakumlu emdirim tekniği ile renklendirildikten sonra kurutulan tavuk eti küplerinin bazı kalite özelliklerinin belirlenmesi amaçlanmıştır. Bu amaca yönelik olarak; tavuk göğüs eti küpleri 25°C’de 2 saat süreyle farklı konsantrasyonlardaki (1, 2 ve 4°Bx) hibiskus ekstraktları emdirilerek renklendirilmiş ve 60°C’de konveksiyonel kurutucuda kurutulmuştur. Çalışmada kullanılan hibiskus ekstraktlarının pH değerleri 2.07-2.27 aralığında belirlenmiştir. Hibiskus ekstraktı kullanılarak elde edilen renkli küplerin nem içeriği %33.77-35.18 ve pH değeri 4.42-5.06 aralıklarında belirlenmiştir. Yüksek hibiskus konsantrasyonunda (4°Bx), et küplerinin daha koyu renkli ve toplam monomerik antosiyanin miktarının ise daha yüksek (50.75 mg/kg) olduğu tespit edilmiştir. Ayrıca, konsantrasyon arttıkça, örneklerin toplam fenolik madde miktarı ve antioksidan aktivitesinin de yükseldiği belirlenmiştir. Çalışma sonuçları, tavuk etinin hem renklendirilmesi hem de fonksiyonel bileşikler ile zenginleştirilmesinde vakumlu emdirim tekniğinin başarıyla kullanılabileceğini göstermektedir. Bununla birlikte arzu edilen son ürün özelliklerine göre uygulama şartlarının optimize edilmesi önem arz etmektedir.

The toxicological effects of Eryngium foetidum extracts on zebrafish embryos and larvae depend on the type of extract, dose, and exposure time.

Eryngium foetidum is a herbaceous plant found in tropical and subtropical regions. In vivo pharmacological parameters show that leaf extracts of this plant have antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities due to their bioactive compounds such as flavonoids and phenols. Despite the evidence for several bioactivities of E. foetidum, information on its safety and tolerability is limited. The objective of this study was to assess the effect and concentration of different extracts of E. foetidum on the development of zebrafish (Danio rerio) embryos. To study the impact of aqueous (AE), ethanolic (EE), and methanolic (ME) extracts, the embryos were exposed to 0.625, 1.25, 2.5, 5, and 10mgmL-1 for up to 120-h postfertilization to assess embryonic developmental toxicity and then to 0.039, 0.078, 0.156, 0.312, and 0.625mgmL-1 to assess the antioxidant responses of the enzymes superoxide dismutase catalase, glutathione S-transferase (GST), and cell apoptosis. The results showed that, depending on the extraction solvent, concentration used, and exposure time, E. foetidum extracts caused mortality, altered the hatching time, and promoted changes in enzymatic activities. Delays in development and increased GST activity were found in all treatments. Apoptosis was not observed in any of the treatments. In conclusion, AE, EE, and ME concentrations above 0.625mgmL-1 can cause adverse effects on the early stages of zebrafish development.

EXPLORING THE PHYTOTOXIC EFFECTS OF Glycyrrhiza glabra L. ON EMERGENCE AND SEEDLING GROWTH OF Pisum sativum L.

Chemical weed control method may cause environmental hazards and residual effects in crops and soil. Alternate approach to control weeds is getting attentions in sustainable production system. Use of aqueous extracts of crops is getting scientific attention as eco-friendly alternative to synthetic herbicides especially under scenario of fast increasing herbicide resistance in weeds. Therefore, the present study was conducted at Weed Science Laboratory, Department of Agronomy, University of Agriculture, Faisalabad during winter 2018 to determine the phytotoxic effects of Glycyrrhiza glabra on winter vegetable crop Pisum sativum. The experiments were laid out under a completely randomized design (CRD), having three replicates. The experiments comprised of seven treatments with different concentrations of extract. The wood of G. glabra extracts comprised 0%, 0.25%, 0.5%, 10%, 20%, 40%, and 80% concentration applied on Pisum sativum. Data on seed germination, root length, shoot length, and fresh weight of weed and pea was recorded following standard procedures. Data were analyzed using Fishers Analysis of Variance. The differences among treatments were separated using a least significant difference test (LSD). Results revealed that aqueous extracts were possessed phytotoxic chemicals that cause phytotoxic effect to P. sativum and result in maximum germination percentage (%), Mean Germination time (Days), Time to 50% germination and minimum, root length (mm), shoot length (mm), root fresh weight (mg), shoot fresh weight was recorded at 80 % concentration of G. glabra. It is concluded that there are phenolic compounds in the wood extract of G. glabra which cause allelopathic effects on P. sativum.

Evaluation of functional and bioactive properties of crude gill extract of Tor putitora using different assays

SummaryFor centuries, natural products isolated from animals, plants and microbes have been the centre of attention for the world's scientific community for their role in eradicating and reducing many diseases. The present research work is also performed on the crude gill extract of Tor putitora named TPG‐Ex to analyse its various functional and bioactive properties. A total of five different biological assays were performed. Two assays performed were the free radical scavenging assays having other free radical generators such as DPPH and H2O2. Different concentrations of extract were used in both assays. The maximum scavenging ability in the Antioxidant DPPH assay was 59.42% at 50 μL. In the H2O2 radical scavenging assay, the maximum scavenging ability of TPG‐Ex recorded was 55.46% at a concentration of 50 μL. The antiangiogenic potential of the TPG‐Ex CAM assay was assessed, which also showed maximum inhibition of neovascularisation at a concentration of 80 μL. The antidiabetic potential of TPG‐Ex was also revealed after processing the extract through glucose uptake by yeast cells assay and glucose adsorption assay. TPG‐Ex indicated maximum glucose uptake at a concentration of 20 μg of 56.61%.

Formulation and Evaluation of Antibacterial Gel containing Ethanol Extract of Thorns of Bombax ceiba

Purpose: Formulation and evaluation of the herbal gel preparation from the thorn’s extract of Bombax ceiba to check its antibacterial activity against the bacteria Staphylococcus aureus and Propionibacterium acnes. Methods: Agar well diffusion method were employed for the purpose. Results: Gel formulation of different concentrations of extract were formulated that is 2, 4, 6 and 8%, respectively and antibacterial activity of the gels were measured against the bacteria S. aureus and P. acnes. In this clindamycin gel was used as the standard for comparative analysis. It was concluded from evaluation results that a formulation of 8% showed better antibacterial activity than other formulated preparations. In addition to this, gel formulations were evaluated considering various parameters such as pH, appearance, viscosity, spreadability and homogeneity and the result were calculated. Conclusion: The ethanol thorn extract of B. ceiba possess good antibacterial property against the S. aureus and P. acnes. It also contains various phytoconstituents which may be helpful in various health-related problems.

Comparative studies of antibacterial activity of Ocimum Sanctum and curcuma longa leaf extracts against E. coli and Staphylococcus Aureus

Due to their traditional therapeutic use, Ocimum Sanctum (Tulsi) and Curcuma Longa (Turmeric) have been subjected to numerous antibacterial tests. To compare the antibacterial effects of tulsi (Ocimum sanctum) as well as Curcuma Longa (Turmeric) against Gram-positive (Staphylococcus aureus) as well as Gram-negative (Escherichia coli) bacteria. The method of agar well diffusion was used. In addition, different phytochemical tests were carried out. Aside from the froth test, tannin tests; Sterol tests showed positive. All of the results are presented in the form of a graph and various tables. When turmeric was used, it exhibited a 22 mm Zone of Inhibition (ZOI) in E. coli and a 17 mm ZOI in Staphylococcus aureus. When tulsi was applied, however, roughly 20mm of ZOI was found in both E. coli and Staphylococcus aureus. At six different concentrations, the diameter of ZOI was measured, methanol extract of both the Turmeric and tulsi, including 0.2 g/ml, 0.3 g/ml, 0.4 g/ml, 0.5 g/ml, 0.6 g/ml, and 0.7 g/ml.In conclusion, Tulsi leaf extract and Turmeric curcumin extract were collected to perform various antibacterial activities. The agar well diffusion method was used in this study. The bactericidal activity of different concentrations of extracts varied significantly.

Preparation and characterization of curcumin and pomegranate peel extract chitosan/gelatin-based Films and their photoinactivation of bacteria

The development of photoantimicrobial films for food and medical devices decontamination is a continuous challenge. In this work, chitosan/gelatin-based films containing curcumin and pomegranate peel extract in different concentrations (0.5–5.0 mg photosensitizer g−1 solution) were prepared and characterized by ultraviolet–visible spectroscopy, fluorescence spectroscopy, infrared spectroscopy and scanning electron microscope; their structural morphology (thickness and moisture content, solubility and swelling degree, and opacity) was also analyzed. The photobleaching results (up to 90%) demonstrated a potential application of the films in antimicrobial photodynamic therapy (aPDT). In the antimicrobial tests, the films displayed a significant photoantimicrobial effect reducing Staphylococcus aureus up to 4 log units, using a light source at 450 and 525 nm (50 J cm−2) for curcumin and pomegranate peel extract films, respectively. The results of this work open way for future development of photoantimicrobial films based on natural polymers, containing photosensitizers, for medical devices (e.g.: endotracheal tubes and catheters) and food packaging.

Orodispersible films based on jambolan juice and natural extracts: Development, characterization and sensory evaluation

AbstractBackgroundThe orodispersible films (ODFs) are ultra‐thin films that dissolve rapidly within the oral cavity. These can be used to carry vitamins, nutraceutical compounds and drugs. Generally, its formulated with synthetic polymers and artificial colorants. Due to growing consumer interest in healthier products, it is necessary to study the use of natural materials for ODF production, such as fruit juices and natural extracts. In this study, ODFs were prepared using pectin, jambolan juice and extracts of guaco, pomegranate, clove or cinnamon in different concentrations. The impact of the addition of natural extracts in different concentrations was evaluated by the following properties of the films: swelling, disintegration time, tensile strength, elongation at break, Young's modulus, work of adhesion, visual and sensory.ResultsThe films showed a visually homogeneous purple color, slowly disintegrating (from 1.1 ± 0.1 to 2.2 ± 0.1 min), flexible, and adherent enough independently of the extract used. The anthocyanin present in the jambolan juice (6.58 ± 0.02 mg g−1) contributed higher antioxidant capacity shown by the ODF (198–292 μMol TEg−1). The sensory evaluation reveals that the films presented adequate acceptance for a new kind of product.ConclusionThe results indicate the use of jambolan juice and natural extracts is suitable for the formulation of ODFs as a healthy product, maintaining technological properties.

Origanum vulgare extract as a natural additive in fresh cheese

Oregano (Origanum vulgare) is a spice that has in its extracts and essential oils biological activities of interest, such as antimicrobial and antioxidant. These qualities are in line with the increased interest in natural alternatives for preserving foods such as fresh cheese due to its short shelf life. The objective of this research was to evaluate the acceptance, antibacterial and antioxidant activity of the hydroalcoholic extract of O. vulgare incorporated in fresh cheese during cold storage. For this, cheeses were produced with different concentrations of extract (0, 0.5, 1 and 3 g of extract/kg). Acceptance was assessed using a nine-point hedonic scale. The presence of Salmonella was investigated and the enumeration of coagulasepositive staphylococci, mesophilic, psychrotrophic and thermotolerant coliforms was performed in the samples. The antioxidant activity was evaluated in vitro (DPPH and FRAP assays) and directly in the food (measuring the malondialdehyde content). The data revealed a good acceptance of all cheeses regardless of the oregano concentration (mean 7.0 to 7.7). The addition of 0.3 g of oregano extract/kg of cheese promoted the inhibition of thermotolerant coliforms during the storage period. Antioxidant activity was demonstrated in vitro but not verified in the cheese. Foods have a complex composition, and the concentration and proportion of different constituents may have influenced the biological actions of the extract. Thus, further research becomes necessary, with variation in storage time and concentrations to know the action of this extract directly in the food model.

A Green Approach for the Biosynthesis of Gold Nanoparticles Using Cuminum cyminum L. Seed and Its Application for Pain Management in Rats.

Background:This study investigated the antinociceptive effect of cumin and its biosynthesized AuNPs. Methods:Cumin extract (E) and cumin-AuNPs (GN) were prepared and administered intraperitoneally at the concentrations of 200, 500, and 1000 mg/ml to 27 male rats. UV–Vis spectroscopy and AFM were applied for AuNPs synthesis confirmation. The nociceptive behavior was assessed, and IL-6 serum levels were measured. Results:Cumin-AuNPs showed a peak absorption of 515 nm, and a size of about 40 nm. Three different concentrations of extract had no significant effect on acute and chronic nociceptive behavior. GN + E200 (46.00 ± 10.59) showed a significant acute anti-nociceptive effect compared to the control (98.66 ± 4.91; p = 0.029) and SS300 (98.33 ± 20.30; p = 0.029) groups. Also, GN + E500 (42.00 ± 11.84) significantly reduced acute nociceptive behavior compared to the control (98.66 ± 4.91; p = 0.019), SS300 (98.33 ± 20.30; p = 0.020), and GN + E1000 (91.00 ± 26.00; p = 0.040) groups. IL-6 serum levels reduced significantly in GN + E500 (24.65 ± 10.38; p = 0.002) and SS300 (33.08 ± 1.68; p = 0.039) compared to the controls (46.24 ± 3.02). Chronic nociceptive behavior was significantly lower in the SS300 (255.33 ± 26.30) compared to E200 (477.00 ± 47.29; p = 0.021), E500 (496.25 ± 46.29; p = 0.013), and GN + E500 (437.00 ± 118.03; p = 0.032) groups. Conclusion: Our findings suggest the potential effects of cumin-AuNPs on formalin-induced nociceptive behavior, which is independent of IL-6serum levels.

Development and in-vitro characterization of HAP blended PVA/PEG bio-membrane

Hydroxyapatite HAP is a bioceramic that can be applied for bone tissue regeneration. Polyvinyl Alcohol (PVA)/Polyethylene Glycol (PEG) polymers are water-soluble and have faster biodegradation properties. The PVA-PEG-HAp thin membrane was cast by using the transparent solution petri-dish method. The PVA-PEG-HAp thin membrane was investigated by Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), Energy Dispersive X-Ray Analysis (EDX), and Field Emission Scanning Electron Microscope (FESEM. Both PVA and HAp of membrane matrix had typical peaks at 2Ɵ value 19.78. PEG also has a distinctive peak at 26.687. The five (5, 25, 50, 75, and 10 μl) different concentrations of extracts of PVA-PEG-HAp thin membrane were pertinent to interacting for 24 h with the L929 mouse cell line, and the results were exhibited a maximum of 99% and 93% cell viability, 1% and 7% cytotoxicity, respectively. Hence the newly developed polymer biomembrane is a biocompatible alternative for biofilms in medical applications.

Antimicrobial activities of aqueous and ethanolic extracts of some natural spices (garlic, turmeric, thyme and onions) on some clinical isolates

Different types of spices used daily have been documented to have antimicrobial and medicinal value as well. Most bacteria are sensitive to the extracts from plants such as, garlic, onion, thyme and turmeric etc. Spices such as these have been used as antimicrobial agents in their raw form for the treatment of wounds, injuries and joint pains etc. The present study was conducted to investigate the antibacterial activity of these natural spices. Different concentrations of extracts were prepared by using two solvents; water and ethanol, the test organisms used were Escherichia coli and Staphylococcus aureus. All the spices used were effective against the test organisms, but the best activity was shown in garlic, while the least activity was shown in thyme, in general, the ethanolic extracts were more reactive than the aqueous extracts. Natural spices could be a potential source for antimicrobial agents. Validation of natural spices as alternative and complementary medicine can be achieved by several in vivo trials through which the safe use of such products can be assured. This study emphasizes the therapeutic potential of spices and the need to enhance its exploitation on this regard.

Quebracho-Based Wood Preservatives: Effect of Concentration and Hardener on Timber Properties

Tannin polyphenols are produced by plants to protect themselves against natural decay. It is expected that impregnating low-durable timber with tannin extracts of more durable species such as quebracho (Schinopsis balansae) will enhance the durability of the specimens. This biomimetic approach combined with the in situ polymerization of quebracho–hexamine formulations can be a valid alternative to synthetic wood preservatives. In this work, we aim to evaluate the impregnation mechanism as well as the impact of tannin and hardener concentration on the mechanical and leaching resistance properties of treated wood. Compression resistance, surface hardness and leaching resistance of four different common non-durable wood species: spruce (Picea abies), pine (Pinus spp.), poplar (Populus alba) and beech (Fagus sylvatica) impregnated with different concentrations of extract and hexamine are presented. The results show that the mechanical properties of tannin-impregnated timber are enhanced, especially for timber with lower densities. Tannin and hardener concentrations tendentially do not contribute significantly to further increase MOE (modulus of elasticity), MOR (modulus of rupture) and Brinell hardness. Similar results are also obtained when the specimens are tested against leaching: tannin is significantly more water-resistant when cured with hexamine, but higher amounts of hardener do not further improve its water resistance. These findings suggest that quebracho tannin–hexamine formulations are already effective at low concentrations (5 to 10% extract with 2.5 to 5% hexamine).

Antibacterial Activity of Boswellia carterii Aqueous Extract and Its Effect on Phagocytosis in vitro.

Boswellia serrate has been traditionally used for the treatment of several inflammatory diseases, and bacterial resistance to antibiotics has recently increased the use of bioproducts. The present study aimed to assess the antibacterial effect and phagocytic ability of the aqueous extract of the Boswellia serrata in bacteria isolated in nosocomial infections. Boswellia carterii plant was collected and prepared from the aqueous extract in different concentrations. A total of 125 samples were collected from various clinical sources, including urine, sputum, wounds, otitis, and blood, from patients of both genders in different age groups. The results demonstrated that out of the 59 infected samples, urine samples had the highest infection (68%), followed by wounds, sputum, and otitis reported as (60%), (44%), and (40%), respectively. On the other hand, blood samples had the lowest percentage of infection (28%). Microscopic diagnostic results, biochemical tests, API Staph System, API 20E System, and Vitek 2 Compact pointed out that the highest infection rates were related to Staphylococcus aureus (32.20%), Pseudomonas aeruginosa (25.33%), and Escherichia coli (22.03%), while the lowest infection rate was detected in Klebsiella pneumonia (20.33%). The results indicated that aqueous extract of Boswellia carterii had an antibacterial activity for all bacterial isolates, 25 mg/ml of extract gave an inhibition zone of 10.8 mm,10.4 mm, 7mm, and 10mm for S. aureus, E. coli, P aeruginosa, and K. pneumonia, respectively, while 200 mg/ml of extract gave 24 mm, 22 mm, 18.4 mm, and 20 mm, respectively. The results pointed to a significant increase in the phagocytosis rate, with the phagocytosis of blood samples treated with Boswellia carterii extract (79.7%), as compared to control samples (57.75%). As evidenced by the results of this study, the aqueous extract of the Boswellia carterii plant showed antibacterial effects and a positive impact on the phagocytic ratio; nonetheless, it is recommended that further studies be conducted to characterize the compounds of this herb.

Potential of vermicompost extract in enhancing the biomass and bioactive components along with mitigation of Meloidogyne incognita-induced stress in tomato.

Increasing inorganic fertilizer and pesticide use has been linked to increased health risks for humans and cattle, as well as substantial water and soil contamination. In recent years, vermicomposting has shown to be a viable alternative to chemical pesticides. Vermicompost and vermicompost products such as extract and leachate assist plants in a number of ways. According to recent studies, vermicompost extract (VCE), when used as a supplement, is thought to work as a growth and stress tolerance booster for plants. These liquid supplements also help to suppress a range of pests, such as root knot nematodes. In the present study, neem- and cattle dung-based vermicompost extracts of different concentrations (0, 20, 40, 60, 80 and 100%) were prepared and used for their application against nematode infection in tomato seedlings under laboratory conditions. Apart from its antagonistic action against Meloidogyne incognita, the influence of VCE on plant growth was investigated by analyzing its morphological characteristics in tomato seedlings infected and uninfected with M. incognita. Seeds were pre-soaked in VCE for the seed priming process before being allowed for germination. After 10days of nematode inoculation, biochemical parameters like protein content, activity of antioxidative enzymes, non-enzymatic antioxidants, stress indices, photosynthetic pigments, proline content and secondary metabolites were also analyzed. The results revealed that neem-based VCE was fatal to second-stage juveniles, with an 82% mortality rate following exposure to the highest dose. When eggs were exposed to 100% VCE, 33.8% of hatching was suppressed, indicating that VCE had an antagonistic effect on nematode egg hatching. Further, all the morphological and biochemical parameters were significantly enhanced in VCE-treated tomato seedlings as compared to untreated seedlings. Stress indices were also found to be significantly lowered by the VCE treatments in the infected plants. The effect of VCE on seedling growth and physiology was shown to be concentration dependent. As a result, the current findings show that VCE has the potential to be used as a plant growth accelerator as well as an environmentally friendly biocontrol agent against nematode pathogenesis in tomato plants.

Evaluation of Antimicrobial Activity of Aqueous Extract of "Ocimum Sanctum-Queen of Herb" on Dental Caries Microorganisms: An In Vitro Study.

AimTo evaluate the efficacy of three different concentrations of Ocimum sanctum aqueous extract against various caries causing microorganisms, Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, and Lactobacillus acidophilus.Materials and methodsAqueous extract of Ocimum sanctum was prepared by the cold extraction method. The extract was diluted with milli Q water, to obtain 3 different concentrations [2%, 3%, and 4%] of the extract. Glycerol 6%v/v and tween-80, 0.1%v/v were also added to get a stable suspension. About 0.2% chlorhexidine was used as a positive control and milli Q water was used as a negative control. The extract, along with the controls, was then subjected to microbiological investigation to determine which concentration among the three different concentrations of extract gave a wider inhibition zone against Streptococcus mutans, Streptococcus mitis, Streptococcus sanguis, and Lactobacillus acidophilus. The zones of inhibition were measured in millimeters.ResultsOcimum sanctum aqueous extract demonstrated maximum antimicrobial activity against microorganisms responsible for dental caries at the 4% concentration level although 3% and 2% were also effective. Maximum activity was seen against S. mutans and S. sanguis with 4% extract.ConclusionOcimum sanctum aqueous extract was effective against all caries causing the microorganisms.Clinical significanceDental caries is still a major oral disease in children which affects their quality of life. It is important to come up with an alternative oral hygiene aid which is easily available and with lesser side effects and maximum benefits by acting against caries causing microbes. Thus aqueous extract of commonly available tulsi is studied to know its effect on caries causing microorganisms.How to cite this articlePai KR, Pallavi LK, Bhat SS, et al. Evaluation of Antimicrobial Activity of Aqueous Extract of “Ocimum Sanctum-Queen of Herb” on Dental Caries Microorganisms: An In Vitro Study. Int J Clin Pediatr Dent 2022;15(S-2):S176-S179.

Antifungal activity of “Mahogany” ( Khaya senegalensis ) leaf extract against some selected fungi

Effect of leaf aqueous extracts of Khaya senegalensis were determined on disease causing fungi. This work aimed to determine the antifungal effect of Khaya senegalensis leaf extract on the growth of some selected fungi. In this study, the phytopathogenic fungi isolated from the spoiled bread are identified based on morphological and cultural characters. The identification of the fungi was confirmed by pathogenicity tests, the organisms were found to be Mucor spp. and Rhizopus spp. Leaf aqueous extracts of different concentrations (100, 200, 300, 400 and 500 mg mL-1) of Khaya senegalensis were added to the growth media prior to inoculation. The aqueous extracts have inhibited the mycelial growth of the fungi, and this effect gradually increased with an increase in the concentration. It could be emphatically concluded that the tested plant extract can effectively control fungi affecting. This makes it potential biofungicide for the management of fungi associated with bread as it is cheap and environmentally safe due to their fungicidal and fungitoxic ability.

Study of Blend of Extracts from Bitter Leaf (Vernonia Amygdalina) Leaves and Banana (Musa Acuminata) Stem as Corrosion Inhibitor of Mild steel in Acidic Medium

This study investigates the effect of concentration of different ratio of the leaves extracts Bitter leaf (Vernonia amygdalina) and Banana stem (Musa Acuminata) on corrosion of mild steel in acidic medium using weight loss method at different temperatures (303 K to 323 K) to determine the inhibitory potentials of the extracts. The FTIR and SEM were used to determine the functional groups and morphology of the mild steel respectively during the experiment. The results showed that the inhibition efficiency (IE) increased with an increase in the concentration of the inhibitor (0.1 g/l to 0.5 g/l) but decreased with an increase in temperature (303 K to 323 K) for all the ratios of bitter leaf and banana stem extracts used. The results also showed that the inhibition efficiency increases with an increase in the concentration and shows maximum inhibition efficiency of (80.85%) at optimum concentration (0.5 g/L) The Energy Dispersive X-ray Spectroscopy (EDS) spectra of the mild steel showed that the composition of Carbon and Oxygen increased by 4% and 24.31%, respectively. The increase might probably be attributed to the presence of (CH2) in CH2OH group. The presence of cavities and pits in the Scanning Electronic Microscope (SEM) images of mild steel showed the damages on the mild steel by corrosion medium. The corrosion rate of mild steel in 1M HCl decreased with an increase in the concentration of the extracts for the different concentrations of extracts used, 100% bitter leaf extract (BLE) has the highest corrosion inhibitory efficiency; this was closely followed by 1:4 blends of bitter leaf extract (BLE) and banana extract (BNE). 100 % BNE extract has the lowest inhibitory efficiency for the different ratios of extracts concentration used. Bitter leaves extracts have better inhibitory efficiency than Banana stem extracts.

Study of Blend of Extracts from Bitter Leaf (Vernonia Amygdalina) Leaves and Banana (Musa Acuminata) Stem as Corrosion Inhibitor of Mild steel in Acidic Medium

This study investigates the effect of concentration of different ratio of the leaves extracts Bitter leaf (Vernonia amygdalina) and Banana stem (Musa Acuminata) on corrosion of mild steel in acidic medium using weight loss method at different temperatures (303 K to 323 K) to determine the inhibitory potentials of the extracts. The FTIR and SEM were used to determine the functional groups and morphology of the mild steel respectively during the experiment. The results showed that the inhibition efficiency (IE) increased with an increase in the concentration of the inhibitor (0.1 g/l to 0.5 g/l) but decreased with an increase in temperature (303 K to 323 K) for all the ratios of bitter leaf and banana stem extracts used. The results also showed that the inhibition efficiency increases with an increase in the concentration and shows maximum inhibition efficiency of (80.85%) at optimum concentration (0.5 g/L) The Energy Dispersive X-ray Spectroscopy (EDS) spectra of the mild steel showed that the composition of Carbon and Oxygen increased by 4% and 24.31%, respectively. The increase might probably be attributed to the presence of (CH2) in CH2OH group. The presence of cavities and pits in the Scanning Electronic Microscope (SEM) images of mild steel showed the damages on the mild steel by corrosion medium. The corrosion rate of mild steel in 1M HCl decreased with an increase in the concentration of the extracts for the different concentrations of extracts used, 100% bitter leaf extract (BLE) has the highest corrosion inhibitory efficiency; this was closely followed by 1:4 blends of bitter leaf extract (BLE) and banana extract (BNE). 100 % BNE extract has the lowest inhibitory efficiency for the different ratios of extracts concentration used. Bitter leaves extracts have better inhibitory efficiency than Banana stem extracts.

Phytochemical Screening, Acute Toxicity and Anti-Rabies Activities of Extracts of Selected Ethiopian Traditional Medicinal Plants

Background: Croton macrostachyus, Justicia schimperiana, and Ricinus communis have been widely used for the traditional treatment of rabies in Ethiopia. The objective of this study was to investigate the phytochemical constituents, acute toxicity, and antirabies activity of crude extracts of the leaves of Justicia schimperiana and Ricinus communis and the stem bark of Croton macrostachyus. Methods: In studying the presence of phytochemicals within each plant extract, standard procedures were used. The Organization for Economic Corporation and Development (OECD) Guideline No.423 was used for the determination of acute toxicity and antirabies activities of extracts towards Swiss albino mice. Different concentrations of extracts were tested for their cytotoxic effect on Vero cells through 3-(4, 5-Dimethylthiazol-2-yl)-2, 5Diphenyltetrazolium Bromide (MTT) assay. The antirabies assay was carried out based on the minimal toxic concentration of extracts. Results: The phytochemical constituents identified were alkaloids, flavonoids, phenols, steroids, tannins, and terpenoids. All the extracts were slightly toxic in the Swiss albino mice model but noncytotoxic in Vero cell lines. The antirabies assay result showed that all plant extracts had a moderate to good antirabies potential. The methanol extracts exhibited an appreciable antirabies activity compared to the other extracts under investigation. Conclusion: The present study concluded that the studied plant extracts have possessed different phytochemicals and have shown appreciable antirabies activities. Hence these plants could be a potential source of the novel antirabies compound and also may be effective against other viruses.