Different Areas Of Spain Research Articles

Clinical implementation of a multiplex PCR detection method for HPV causing plantar warts and genotype distribution in different geographical areas of Spain

Human papillomavirus is the ethological agent of various tumors, including plantar warts as one of the most frequent clinical presentations. Diagnosis of these warts continues to be mainly clinical, and a significant incidence of misdiagnosis leads to inadequate treatment. The aim of this study is to implement and validate a multiplex PCR detection method in the clinical setting to detect HPV in samples and to study genotype distribution in Spain to improve future molecular diagnostics. Viral DNA was extracted from 128 samples of clinically suspected plantar warts from various locations in Spain. A multiplex PCR was run alongside internal controls, and amplicons were processed for sequencing and HPV genotyping. The method was validated by assessing both inter- and intra-run repeatability. The PCR detection method returned 81.2 % (n = 104) positive results in the samples tested. Inter- and intra-run repeatability tests showed excellent intra-run agreement (κ = 1.00, p < 0.001) and good inter-run agreement (κ = 0.737, p < 0.001). The most frequent HPV type was HPV1, followed by HPV27, showing a statistical difference between the distribution of HPV genotypes in different areas of Spain. Clinical implementation of a DNA PCR detection method for plantar warts can avoid 18.8 % of unnecessary treatments in doubtful cases, and the method is reliable and validated for the purpose. HPV types show an asymmetric geographical distribution that should be considered for diagnosis and treatment.

Analysis of the Influence of Socio-Demographic Variables and Some Nutrition and Lifestyle Habits on Beverage Consumption in the Spanish Population.

Beverages and drinks play a significant role in maintaining the integral health of individuals. The aim of this study is to discover the pattern of beverage consumption in different groups of the Spanish population and to investigate its relationship with other nutritional variables and habits. To achieve the objectives, an observational, descriptive and cross-sectional study was conducted. For data collection, a questionnaire was designed and validated that explored different beverage and food consumption variables as well as socio-demographic and lifestyle variables. The instrument was disseminated, among the Spanish young adult population, through snowball sampling using social networks, collecting a sample of 17,541 valid surveys. Bivariate comparative analyses and correlation analyses were performed, and finally, the principal component analysis (PCA) method was used in order to study the relationships between variables related to drinking and health. The main results show significant differences in the pattern of beverage consumption between the socio-demographic variables of sex, age and educational level, as well as between different areas of Spain, while the PCA model shows the relationship between the consumption of sugar-sweetened beverages with the Healthy Nutrition Index of the population and sport practice. Based on the results of the study, the following conclusions were reached: the beverage consumption pattern of the Spanish population is affected by socio-demographic variables. Healthier drinking habits affect the nutrition and health of the population.

Serological survey of Leishmania infantum in apparently healthy dogs in different areas of Spain.

Canine leishmaniosis caused by Leishmania infantum is an endemic disease in Spain. The dog is considered the main reservoir, and the detection of specific serum antibodies against L. infantum antigen is the most used technique for diagnosing this infection. The LEISCAN LEISHMANIA ELISA test is a commercialized enzyme-linked immunosorbent assay for the detection and measurement of canine anti-Leishmania serum antibodies. The aim of this study was to assess seroprevalence results of apparently healthy dogs in different areas of Spain using LEISCAN. Collection of sera from 5451 apparently healthy dogs was performed between 2020 and 2021 in different areas of Spain. Dogs were of adult age (≥12 months), were not previously diagnosed with clinical leishmaniosis or vaccinated against Leishmania and did not present clinical signs compatible with L. infantum infection. LEISCAN was performed following the manufacturer's protocol. The overall seroprevalence was 5.5%. The highest seroprevalences were found in the Southeast of Spain: Comunidad Valenciana (14%) and Región de Murcia (14%), whereas the lowest seroprevalences were found in Northern Spain: Galicia (1%), Navarra (2%) and Castilla y León (2%) (p-value <0.001). In conclusion, the seroprevalence for L. infantum in apparently healthy dogs in Spain varied from almost no infection to being over 10%.

Atrial fibrillation as a new prognosis factor in chronic patients after hospitalization: the CHRONIBERIA index

A collaborative project in different areas of Spain and Portugal was designed to find out the variables that influence the mortality after discharge and develop a prognostic model adapted to the current healthcare needs of chronic patients in an internal medicine ward. Inclusion criteria were being admitted to an Internal Medicine department and at least one chronic disease. Patients’ physical dependence was measured through Barthel index (BI). Pfeiffer test (PT) was used to establish cognitive status. We conducted logistic regression and Cox proportional hazard models to analyze the influence of those variables on one-year mortality. We also developed an external validation once decided the variables included in the index. We enrolled 1406 patients. Mean age was 79.5 (SD = 11.5) and females were 56.5%. After the follow-up period, 514 patients (36.6%) died. Five variables were identified as significantly associated with 1 year mortality: age, being male, lower BI punctuation, neoplasia and atrial fibrillation. A model with such variables was created to estimate one-year mortality risk, leading to the CHRONIBERIA. A ROC curve was made to determine the reliability of this index when applied to the global sample. An AUC of 0.72 (0.7–0.75) was obtained. The external validation of the index was successful and showed an AUC of 0.73 (0.67–0.79). Atrial fibrillation along with an advanced age, being male, low BI score, or an active neoplasia in chronic patients could be critical to identify high risk multiple chronic conditions patients. Together, these variables constitute the new CHRONIBERIA index.

Spanish wastewater reveals the current spread of Monkeypox virus

Besides nasopharyngeal swabs, monkeypox virus (MPXV) DNA has been detected in a variety of samples such as saliva, semen, urine and fecal samples. Using the environmental surveillance network previously developed in Spain for the routine wastewater surveillance of SARS-CoV-2 (VATar COVID-19), we have analyzed the presence of MPXV DNA in wastewater from different areas of Spain. Samples (n=312) from 24 different wastewater treatment plants were obtained between May 9 (week 19 of 2022) and August 4 (week 31 of 2022). Following concentration of viral particles by a validated aluminum adsorption-precipitation method, a qPCR procedure allowed us to detect MPXV DNA in 56 wastewater samples collected from May 16 to August 4, 2022, with values ranging between 2.2×103 to 8.7×104 genome copies (gc)/L. This study shows that MPXV DNA can be reproducibly detected by qPCR in longitudinal samples collected from different Spanish wastewater treatment plants. According to data from the National Epidemiological Surveillance Network (RENAVE) in Spain a total of 6,119 cases have been confirmed as of August 19, 2022. However, and based on the wastewater data, the reported clinical cases seem to be underestimated and asymptomatic infections may be more frequent than expected.

Family, school, and crossroads

In this article, we analyse the language regimentation of multilingual repertoires and plurilingual practices of young people of Moroccan origin in three different areas of Spain – Barcelona, Galicia, and Madrid. By focusing on the family and the school as two highly interconnected settings (Heller, 2012) which have an impact on their socialisation trajectories and on their development of functional bi/multilingualism – and, thus, on their linguistic practices – we consider, on the one hand, how these young people position themselves regarding multilingualism and the conception of a local and transnational plurilingual speaker and, on the other, how they invest their linguistic repertoire in the transnational space or what Hornberger (2005) calls the ‘implementational spaces’ of multilingual practices. Following a sociolinguistic and discursive critical analysis of the data collected (ethnographic data, the results of several didactic activities, focus groups and interviews), the results point out how family and school discourses surrounding multilingualism contribute to maintaining a situation of multilingualism, which includes both the heritage languages and the institutional and national languages and varieties. Moreover, the results show the ways these plurilingual youth navigate this multilingualism but also how they reconstruct it by deploying hybrid and creative practices.

Editorial introductions.

Editorial introductions.

Bullying, basic psychological needs, responsibility and life satisfaction: connections and profiles in adolescents

The goal was to assess the interactions between bullying and adolescents’ basic psychological needs, responsibility and life satisfaction. 1785 students from secondary education (year eight, n = 404; year nine, n = 390; year 10, n = 364; year 11, n = 376), and Baccalaureate (year 12, n = 251), from 16 schools located in three different areas of Spain: north (Asturias), central-north (Leon), and central-south (Cuenca y Albacete) agreed to participate. 590 answered the questionnaire used referring to Math, 596 to Literature and 599 to Physical Education. Results showed five profiles: one adaptive (class three) with high levels of basic psychological needs satisfaction, low levels of basic needs frustration, and high levels of life satisfaction, personal and social responsibility, and the lowest levels of victimization and aggression, and four other less adaptive profiles. Therefore, the satisfaction of these needs must be promoted in the schools to prevent and/or mitigate bullying problems.

Editorial introductions

Editorial introductions

Fusarium mycotoxins in total mixed rations for dairy cows.

Mycotoxins produced by certain fungal species of the Fusarium genus are frequently found as contaminants in cereals and feedstuffs. Fumonisins (FBs), deoxynivalenol (DON) and zearalenone (ZEN) are of special concern relative to animal health and productivity. The aim of this work was to analyse the levels of Fusarium mycotoxin contamination in samples of total mixed rations (TMRs) for dairy cows. To accomplish this analysis, an HPLC-MS/MS multi-mycotoxin method was developed and validated. The relation between the formulation of TMR samples and the presence of mycotoxins was also studied. From February 2016 to January 2018, a total of 193 TMR samples for dairy cows collected from farms located in different areas of Spain were analysed for the presence of FBs, ZEN, DON and their metabolites. In total, 112 samples (58%) were contaminated with at least one mycotoxin, and 38 samples (20%) presented more than one mycotoxin. FBs were the mycotoxins most frequently found (34% positive samples). DON was detected in 17% of samples, and ZEN was detected in 16% of samples. Among the metabolites analysed, only deoxynivalenol-3-glucoside (DON-3-Glc) and 15-acetyldeoxynivalenol (15-ADON) were detected. The levels of all the Fusarium mycotoxins studied were always below the values recommended by the European Commission for feedstuffs. The wide variety of ingredients used in the formulation of the analysed samples made it difficult to reach definite conclusions, although it seemed that some cereal silages and concentrates such as cereals or compound feed used as ingredients of the TMR may be related to the presence of mycotoxins.

A Spanish Intentional Mass-Casualty Incidents Medical Response Model: Delphi Consensus.

The increase in global violence in recent years has changed the paradigm of emergency health care, requiring early medical response to victims in hostile settings where the usual work cannot be done safely. In Spain, this specific role is provided by the Tactical Environment Medical Support Teams (in Spanish, EMAETs). The Victoria I Consensus document defines and recognizes this role, whose main lines of work are the emergency medical response to the tactical team and to the victims in areas under indirect threat, provided that the tactical operators can guarantee their safety. To reinforce the suitability of this approach, we submitted the possible outcomes of this response model to a panel of national experts to assess this proposal in the different areas of Spain. The chosen research design is a conventional Delphi method, based on the content of the Victoria I Consensus response model. The panel of 52 expert reviewers from 11 different regions were surveyed anonymously; a high degree of accord was recognized when the congruence of the responses exceeded 75%. Consensus agreement was reached in all sections of the survey after two iterations. Specific contributions and recommendations were made to achieve unanimous consensus despite the population and resource differences in the country. Our results suggest that the EMAET approach is useful in areas with short response times. However, in more sparsely populated areas, this may not be feasible, and a more pragmatic response model may be suitable.

Editorial introductions

Editorial introductions

Helicobacter pylori seroprevalence in Spain: influence of adult and childhood sociodemographic factors.

Helicobacter pylori (H. pylori) chronic infection causes severe digestive diseases, including gastric cancer, and certain strains entail a higher risk. Risk factors for this infection are still not fully understood. The aim of this study was to describe the association of adult and childhood sociodemographic factors with the seroprevalence of H. pylori, and with CagA and VacA antigen-specific seropositivity among H. pylori-seropositive individuals in the Spanish adult population. Serum antibody reactivity to H. pylori proteins was evaluated using multiplex serology in 2555 population-based controls enrolled in the MCC-Spain study, a multicase-control study recruiting participants from 2008 to 2013 in different areas of Spain. H. pylori seroprevalence was defined as seropositivity against at least four bacterial proteins. Information on sociodemographics, lifestyles, and environmental exposures was collected through personal interviews. Prevalence ratios and 95% confidence intervals were estimated using Poisson regression models to assess the association of lifetime sociodemographic factors with H. pylori seroprevalence and with seropositivity for CagA and VacA. H. pylori seroprevalence was 87.2%. Seropositivity was statistically significantly higher in men, increased with age, BMI, and number of siblings, and decreased with education and socioeconomic family level at birth. Among H. pylori-seropositive individuals, seropositivity was 53.3% for CagA, 61.4% for VacA, and 38.8% for both CagA and VacA. Ever smokers had lower seroprevalence for CagA and VacA than never smokers. H. pylori seroprevalence among this Spanish adult population was high and one third of the population was seropositive for two well-known markers of gastric cancer risk: CagA and VacA. Sex, age, education, and BMI were associated with H. pylori seroprevalence.

Wind power ramp event detection with a hybrid neuro-evolutionary approach

In this paper, a hybrid system for wind power ramp events (WPREs) detection is proposed. The system is based on modeling the detection problem as a binary classification problem from atmospheric reanalysis data inputs. Specifically, a hybrid neuro-evolutionary algorithm is proposed, which combines artificial neural networks such as extreme learning machine (ELM), with evolutionary algorithms to optimize the trained models and carry out a feature selection on the input variables. The phenomenon under study occurs with a low probability, and for this reason the classification problem is quite unbalanced. Therefore, is necessary to resort to techniques focused on providing a balance in the classes, such as the synthetic minority over-sampling technique approach, the model applied in this work. The final model obtained is evaluated by a test set using both ELM and support vector machine algorithms, and its accuracy performance is analyzed. The proposed approach has been tested in a real problem of WPREs detection in three wind farms located in different areas of Spain, in order to see the spatial generalization of the method.

Does the public's negative perception towards wood in rivers relate to recent impact of flooding experiencing?

Instream large wood (LW) is widely perceived as a source of hazard that should be avoided. This is also the case of Spain, where wood has been systematically removed from rivers for decades. Consequently, people are accustomed to rivers with minimal or no LW at all. However, the presence and transport of wood is natural and has positive ecological effects. Previous studies reported that the general negative perception towards LW in rivers is related to the lack of background knowledge about stream ecology or fluvial dynamics. However, we hypothesize here that recent flooding experience has an influence on the perception of LW as well. To test this hypothesis, we surveyed groups of individuals living in different areas of Spain that have been affected more or less frequently by floods. In addition, we surveyed a group of scientists to test whether their perception towards LW differs from that of the general public. We observe that flooding experience is not the main controlling factor of how LW is perceived. Instead, we observe that respondents, independently of the time passed since the last flood, perceived watercourses with LW as less aesthetically, more dangerous, and with a larger need to improve channels than in watercourses without LW. Regional differences were detected, potentially related to differences in environmental attitudes. We confirm the existence of a gap in perception between scientific communities and the general public regarding natural river systems with wood; thereby highlighting the need to transfer knowledge, training, and education to bridge this gap. The generalized negative perception towards LW could have important consequences on the implementation of river management measures, such as LW augmentation for restoration purposes. This study underlines that wood removal should be more balanced in post-flood works and that public information is needed to implement a balanced LW management policy.

Nut Allergy in Two Different Areas of Spain: Differences in Clinical and Molecular Pattern.

Introduction: Different clinical and molecular patterns of food allergy have been reported in different areas of the world. The aim of the study is to evaluate differences in allergen patterns among nut-allergic patients in two different areas of Spain. Material and methods: A total of 77 patients with nut allergy from two different regions of Spain (Madrid and Asturias) were evaluated. Results: Hazelnut, peanut, and walnut were the three most frequent nuts eliciting allergy in both regions, but in a different order. Patients from Madrid experienced systemic reactions more often than patients from Asturias (73.5% Madrid vs. 50.0%, p < 0.05). The percentage of sensitizations to LTP (Lipid Transfer Protein) was higher than Bet v 1 (p < 0.05) in the Madrid area. The percentage of sensitizations in Asturias area was similar to LTP than Bet v 1 (Pru p 3 46.4%, Bet v 1 42.9%, ns). Bet v 1 was the predominant allergen involved among hazelnut-allergic patients (56.2%), while LTP was more common in peanut-allergic patients (61.5%). Conclusion: Walnut, hazelnut, and peanut were the most frequent nuts eliciting allergy in Spain. Despite this, important differences in molecular pattern were appreciated not only between both regions, but also among nut-allergic patients in Asturias. The different molecular pattern was linked to the frequency of systemic symptoms.

«Entonces usted niega ¿no? ¿O que no se acuerda?»: estudio cuantitativo de los tipos de preguntas utilizadas en interrogatorios judiciales españoles

Esta investigación se centra en analizar los tipos de preguntas utilizadas en interrogatorios judiciales españoles. Para ello, se han analizado dos corpus de juicios orales celebrados en dos zonas geográficamente dispares de España: Almería y Barcelona. En el presente artículo se argumenta, con datos estadísticos, el frecuente uso de enunciados con fines interrogativos cuya entonación responde a patrones prototípicos aseverativos; esta tipología presenta mayores índices de frecuencia en el corpus recogido en Almería (43.9%) que en el de Barcelona (23.7%).

Determination of fluoroquinolone antibiotic residues in the plasma of Eurasian griffon vultures (Gyps fulvus) in Spain

Due to the possible toxicological impact, the accumulation of pharmaceuticals in wildlife as a consequence of human practices is of growing concern. The consumption of carrion at feeding stations – the so-called ‘vulture restaurants’ – with no management of the veterinary drugs it contains may expose scavengers to pharmaceuticals. To demonstrate this, we analyzed plasma from Eurasian griffon vultures (Gyps fulvus) originating from two different areas of Spain for antibiotics such as enrofloxacin and ciprofloxacin, its primary metabolite. Quinolone residues were detected in about 65% (n=106) of birds, of which 15.1% (16/106) had quantifiable amounts of enrofloxacin (0.049±0.102μg/mL) and 5.7% (6/106) of ciprofloxacin (0.009±0.007μg/mL). The differences in exposure between the two sampled areas are attributable to different types of carrion management: the vultures that fed in areas with a high density of dead livestock (supplied directly to feeding stations) were more prone to exposure than those that sought food in areas where carcass availability is more unpredictable. Our findings are evidence that vultures have access to medicated livestock and that there are quantifiable levels of livestock antibiotics in vulture plasma. However, the vultures analyzed in this study had maximum antibiotic concentrations of only 0.4μg/mL, much less than the concentrations used in the clinical treatment of scavengers and a level that is probably too small to cause intoxication.

COPPADIS-2015 (COhort of Patients with PArkinson's DIsease in Spain, 2015), a global--clinical evaluations, serum biomarkers, genetic studies and neuroimaging--prospective, multicenter, non-interventional, long-term study on Parkinson's disease progression.

BackgroundParkinson’s disease (PD) is a progressive neurodegenerative disorder causing motor and non-motor symptoms that can affect independence, social adjustment and the quality of life (QoL) of both patients and caregivers. Studies designed to find diagnostic and/or progression biomarkers of PD are needed. We describe here the study protocol of COPPADIS-2015 (COhort of Patients with PArkinson’s DIsease in Spain, 2015), an integral PD project based on four aspects/concepts: 1) PD as a global disease (motor and non-motor symptoms); 2) QoL and caregiver issues; 3) Biomarkers; 4) Disease progression.Methods/designObservational, descriptive, non-interventional, 5-year follow-up, national (Spain), multicenter (45 centers from 15 autonomous communities), evaluation study. Specific goals: (1) detailed study (clinical evaluations, serum biomarkers, genetic studies and neuroimaging) of a population of PD patients from different areas of Spain, (2) comparison with a control group and (3) follow-up for 5 years. COPPADIS-2015 has been specifically designed to assess 17 proposed objectives. Study population: approximately 800 non-dementia PD patients, 600 principal caregivers and 400 control subjects. Study evaluations: (1) baseline includes motor assessment (e.g., Unified Parkinson’s Disease Rating Scale part III), non-motor symptoms (e.g., Non-Motor Symptoms Scale), cognition (e.g., Parkinson’s Disease Cognitive Rating Scale), mood and neuropsychiatric symptoms (e.g., Neuropsychiatric Inventory), disability, QoL (e.g., 39-item Parkinson’s disease Quality of Life Questionnaire Summary-Index) and caregiver status (e.g., Zarit Caregiver Burden Inventory); (2) follow-up includes annual (patients) or biannual (caregivers and controls) evaluations. Serum biomarkers (S-100b protein, TNF-α, IL-1, IL-2, IL-6, vitamin B12, methylmalonic acid, homocysteine, uric acid, C-reactive protein, ferritin, iron) and brain MRI (volumetry, tractography and MTAi [Medial Temporal Atrophy Index]), at baseline and at the end of follow-up, and genetic studies (DNA and RNA) at baseline will be performed in a subgroup of subjects (300 PD patients and 100 control subjects). Study periods: (1) recruitment period, from November, 2015 to February, 2017 (basal assessment); (2) follow-up period, 5 years; (3) closing date of clinical follow-up, May, 2022. Funding: Public/Private.DiscussionCOPPADIS-2015 is a challenging initiative. This project will provide important information on the natural history of PD and the value of various biomarkers.

Leucoagaricus viridariorum (Agaricaceae, Agaricales), a new species from Spain

Leucoagaricus viridariorum is proposed as a new species based on material collected in different areas of Spain. This taxon is characterised macroscopically by its small, whitish basidiomes, minutely squamulose-fibrillose pileus, evanescent ascendant annulus and growth in man-made environments. Microscopically, its subglobose to broadly ellipsoid spores, the clavate cheilocystidia and the trichodermic pileipellis are diagnostic. Based on molecular data of the internal transcribed spacer of nuclear ribosomal DNA (nrITS) this species belongs to the Leucoagaricus/Leucocoprinus clade of the Agaricaceae where it is sister to Leucoagaricus amanitoides.