Different Areas Of Bangladesh Research Articles

Prevalence and factors influencing gastrointestinal parasitic infections in sheep in Bangladesh.

Gastrointestinal (GI) parasites are major contributors to decrease productivity in livestock over the world. A cross-sectional study was conducted in different areas of Bangladesh to determine the prevalence of GI parasitic infections and their association with the biotic and abiotic factors in sheep. A total of 572 faecal samples were collected from the selected areas of Bangladesh and microscopic examination was performed for the identification of parasites using flotation and sedimentation technique. Out of 572, 441 animals were found infected with one or more species of GI parasites with an overall prevalence of 77.1%. Nine types of parasites from four different classes were detected namely Strongyles (42.1%), Strongyloides sp. (27.1%) and Trichuris sp. (1.0%), Moniezia sp. (2.4%), Paramphistomum cervi (32.5%), Fasciola gigantica (6.1%) and Schistosoma sp. (3.5%), coccidia (16.6%) and Balantidium coli (7.9%). Nematodes infections (56.8%) were significantly highest among trematodes (37.9%), protozoa (24.4%) and cestode (2.4%). In the present study, all the biotic factors including sex, age, physiological condition of female and body condition score (BCS) of animals were insignificantly (p>0.05) associated with the prevalence of GI parasitic infection in sheep but among the abiotic factors, muddy housing of animals, rainy season, having no knowledge about GI parasites and illiteracy of farmers were significantly (p<0.05) associated with the GI parasitic infections. This epidemiological investigation will assist to build a suitable control program against GI parasites in sheep and thus, help to prevent production loss and increase livelihood of small holder farmers.

Estimation of aflatoxin levels in selected spices in different areas of Bangladesh

Estimation of aflatoxin levels in selected spices in different areas of Bangladesh

Assessment of toxic and essential metals in fish feed ingredients available in different areas of Bangladesh

Contamination of heavy metals in fish feed ingredients is regarded as a major crisis globally especially in developing countries because it may be the source of toxicity in the food chain. Fish farming in Bangladesh is growing very rapidly and fish available in the markets are mostly coming from fish farms where fish farmers mostly use commercial fish feeds manufacturing with different ingredients. In this study, several types of ingredients based on different origins were collected from several areas and after measuring dry weight these were digested by a mixture of acids. This study is concerned to access the toxic and essential metals in different fish feed ingredients which are frequently used to produce commercial fish feed in Bangladesh. The concentration (mg kg-1 dry weight) range of toxic metals such as; Pb (0.56- 1.73), Cd (0.12-0.97), Cr (0.15-0.88), As (BDL), Hg (BDL), Ni (1.10-2.50) and essential metals such as; Fe (13.57-48.96), Cu (10.11-28.09), Zn (10.60-26.25), Na (12.07-35.00), K (13.06-28.97), Ca (10.00-47.96) in selected fish feed ingredients were recorded by Inductive Coupled Plasma Optical Emission Spectroscopy (ICP-OES, Optima 7000DV) with significant variation (P&amp;lt;0.05). The analyzed toxic and essential metal concentrations in most of the ingredients were within the safe limit proposed by World Health Organization, Food and Agricultural Organization and European Union indicating no health risk.

Evaluation of eight isabgol (Plantago ovata Forsk.) germplasm performance grown under different climatic conditions in Bangladesh

The extent of genetic diversity in the crop plants is of prime concern to plant breeders and germplasm curators. Therefore, a study was employed to determine the genetic diversity and to evaluate the performance of eight genotypes of isabgol (Plantago ovata Forsk.) through analysis of morpho-physiological and yield attributing characteristics. All the germplasm was collected from different areas of Bangladesh. The experiments were conducted at the research field of Regional Spices Research Centre, Magura during the rabi season, 2019-20 to characterize phenotypically and to evaluate the performance of different isabgol germplasm. The experiments were laid out in randomized complete block design with three replications. The highest plant height (42.33 cm), number of tillers per plant (7.33), number of leaves per plant (74), length of leaf (30.00 cm), number of spikes per plant (29.0), length of spike (4.03 cm), 1000 seeds weight (2.0 g) and seed yield (823 kg ha-1) of isabgol was found from the germplasm PO-001 and the lowest plant height (39.67 cm), number of tillers per plant (6.33), number of leaves per plant (69.33), length of leaf (26.33 cm), number of spikes per plant (23.0), length of spike (3.67 cm), 1000 seeds weight (1.87 g) and seed yield (705 kg ha-1) of isabgol was found from the germplasm PO-007. On the basis of this field trail in terms of seed yield and other important agronomic characteristics the genotypes PO-001 can be chosen for future breeding material to release a commercial variety in Bangladesh climatic conditions.

RT-PCR Mediated Identification of SARS-CoV-2 Patients from Particular Regions of Bangladesh and the Multi-factorial Analysis Considering Their Pre and Post Infection Health Conditions

Background: SARS-CoV-2 is a type of coronavirus shows bizarre features with fatality rates at 34.4%. The clinical and non-clinical features of COVID-19 might be causative factors for more rapid community spreading compared to MERS and SARS-CoV-1.&#x0D; Objectives: To study the efficacy of RT-PCR based detection of SARS-CoV-2 patients. Besides, the patients' pre and post-infection health conditions depending on selective clinical and nonclinical parameters were analyzed.&#x0D; Materials and Methods: Clinical and non- clinical data were collected from 205 randomly selected COVID-19 patients, identified through RT-PCR from different areas of Bangladesh. The data were analyzed using statistical and analytical tools to illustrate the impact of the pandemic situation on the sufferers. &#x0D; Results: Molecular identification of the patients through RT-PCR has been observed as the most efficient option for detecting SARS-CoV-2 patients. Patients with the smoking habit have been reported to be more prone to the COVID-19 infection; surprisingly the non-smoker female was the worse suffers. The patients aged 60-69 years were the worst sufferers with every adverse health conditions mentioned. Professionally, 28% self-employed male and 8.19% female were infected. The most infected individuals were house wife (32.029%), as next to the health care workers (22.1%). With the increased age group, a decreased number of smokers were found. Nebulization of 5% home and 12% hospital treated patients were provided, with Oxygen for 26% of hospitalized patients. After recovery, combined physical and psychological complications were observed in 19% of male and 11% female. The socioeconomic, environmental and geographical annotations exposed a clear relationship with the rate of infection as revealed from the nonclinical data analysis.&#x0D; Conclusion: RT-PCR proved its specialty in COVID-19 detection. In addition, impacts of different clinical and nonclinical factors on the physical conditions of the nCoV patients were found significant in the research.

A survey of hand anthropometry of Bangladeshi agricultural farm workers

The target of this investigation was to represent an anthropometric dataset of hand dimensions from agricultural farm workers in two different areas of Bangladesh. A total of 200 farm workers (110 males and 90 females) age between 15 and 52 years-old were included as voluntary participants from two regions of Bangladesh. 27 hand dimensions were measured, recorded, and analysed from the sample populations. The mean, standard deviation, and different percentile esteems of collected data were also estimated and recorded. Moreover, Kolmogorov-Smirnov and t-statistical tests were performed for making the anthropometric comparison among different populaces. Results showed that all the hand dimensions of the males were higher than that recorded for the female participants of Bangladesh. In addition, the outcomes showed noteworthy contrasts in hand measurements among Bangladeshi people and other considered populaces. The result of this examination is expected to influence the design of agricultural hand tools, machinery, and equipment in Bangladesh from industrialized countries. It will also generate the interest of more anthropometric studies in Bangladesh that are related to the design of hand tools or equipment for work or other activities.

Validation of non-electric chick brooding system in different areas of Bangladesh- A new device for rural poultry farmers

The experiment was conducted to validate an alternative chick brooding device developed for rural poultry farmers in 3 Technology Dissemination Area (TDA) of Poultry Technology Development and Dissemination Project (PTDDP) during winter. The device is a locally designed drum type non-electric chick brooder. A total of 8 poultry farmers in two groups (500 and 1000 chicks) used the specially designed brooder of different sizes. The size of the drum (Height, H- 40.64 × Diameter, D- 45.72) and (H-40.64 × D- 60.96) cm found suitable for 500 and 1000 chicks respectively. The diameter of the hover should double of diameter of the drum with 15 cm long down ward lid around the hover and two bottom outlets for each drum. Manufacturing cost and brooding cost were TK. 2250 and TK. 900 for 500 chicks and TK. 1750 and Tk.1350 for 1000 chicks respectively. The drum designed for 500 and 1000 chicks required 25 kg and 35 kg of saw dust in one time filling that could produce heat for 14 and 22 hours, respectively. Better growth performance as well as profitability was found in non-electric chick brooding groups than the brooding with electricity and diesel burner (locally known as Hericane). It may be concluded that the newly developed non electric chick brooding device could be suitable as an alternative chick brooding system for rural poultry farmers, especially in the areas where there is no electricity supply.&#x0D; Bangladesh J. of Livestock Res. 21-25: 178-182, 2018

Determination of Iodine Content of Commercially Available Table Salts at the Retailer Level in Selected Areas of Bangladesh

Aims: Iodine deficiency is one of the most common micronutrient deficiencies in Bangladesh. To combat iodine deficiency disorders, universal salt iodization is mandatory in Bangladesh. The aim of our study was to determine the iodine content of both packaged and open edible table salts sold at the retailer level in different areas of Bangladesh.&#x0D; Study Design: The study is an experimental cross-sectional study.&#x0D; Place and Duration of Study: The present study was conducted in the food analysis laboratory of Department of Food Technology and Nutrition Science, Noakhali Science and Technology University from March 2019 to June 2019. A total of 90 salt samples were collected from ten retailers selected based on convenience sampling from two districts: Dhaka and Noakhali. Among the samples, 45 were packaged salts from Fifteen different brands and the rest of the 45 samples were non brand open salt.&#x0D; Methodology: The iodine content of iodized salt samples was determined by the iodometric titration method.&#x0D; Results: The mean iodine content of both types of salts is 17.801±1.973 ppm. The mean iodine contents of packaged salts and open salts are 30.691±2.679 ppm and 4.912±1.008 ppm, respectively. Only 42% of the total salt samples are adequately iodized (&gt;20 ppm). 75% of packaged salt samples are adequately iodized and only 8% of open salt samples are adequately iodized.&#x0D; Conclusion: As iodine content in open table salts doesn’t meet the criteria set by the government, the sale of open salt for human consumption should be stopped.

Taxonomic account of orb-weaving spider genus Cyrtarachne Thorell, 1868 (Araneae: Araneidae) of Bangladesh

A taxonomic study on some orb-weaving spiders of the genus Cyrtarachne Thorell, 1868 (Araneae: Araneidae) were carried out from different areas of Bangladesh. Two species were identified in this study namely C. biswajiti n. sp. and C. sundari Tikader. C. biswajiti has described as new to science. Illustrated descriptions of the species were provided herewith.&#x0D; J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(2): 75-82

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Staphylococcus aureus is gram-positive cocci that can cause foodborne illness which can be transmitted by chicken meat, both raw and undercooked; consumption of which may cause infection and/or toxicity in consumers. This current study was conducted for the detection of the prevalence of S. aureus in three types of poultry samples which included chicken meat, chicken eggs and droppings. Samples were aseptically collected from different rural and urban areas of 8 districts of Bangladesh in triplicate collection method which was conducted in the Centre for Excellence, Department of Microbiology, Primeasia University, Dhaka, Bangladesh to detect the Multi Drug-Resistant (MDR) S. aureus. Isolation was done by using 7.5% Sodium Chloride broth for enrichment and Mannitol salt agar after enriching samples in Buffer Peptone water. Antibiogram was done by using Kirby Bauer Method on Muller Hinton agar. The current study revealed 68% (17/25), 84% (21/25) and 52% (13/25) of S. aureus in chicken meat, eggs and dropping, respectively. Around 75.47% isolates (40/53) showed 100% similarity with S. aureus from the generated Dendrogram based on biochemical data. Cefixime (62.67%) showed the highest sensitivity against all of the isolates while Penicillin (86.62%) exhibited the highest resistance. Moreover, 59.62% of isolates were resistant to 5 or more drugs (Multidrug-Resistant). Poultry products such as meat and eggs were contaminated with drug-resistant S. aureus which can cause serious health effect to the consumer. The drug-resistant pathogen in droppings helps to indicate the spread of drug-resistant isolates in the environment. Therefore, proper sanitation measures should be taken to ensure the quality of the products during slaughtering, collecting eggs or disposing of poultry litter.

Clinical investigation of PPR outbreak and sero-prevalence of PPR viral antibody in different areas of Bangladesh

Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. This research work was done in 2016-2017 by executing, surveillance and clinical investigation studies to determine present status of circulating PPR virus and its detection of antibody level of PPRV in different areas of Bangladesh. cELISA was conducted to detect the PPR antibody and RT-PCR also used for identification of N gene PPRV. The clinical outbreak of PPR, the total 124 samples was collected at the six locations of the country and highest case fatality (morbidity) was recorded at Jhenaidah 93.75% (75 out of 80).The highest morbidity rate and mortality rate was 69.23% and 13.07% respectively. The result of RT-PCR indicates the PPR virus circulating in the different regions of Bangladesh. For sero-prevalence of PPR antibodies of 366 serum samples were collected at different region of Bangladesh such as Chuadanga 47.81% (22 out of 47), Sirajganj 34.21% (13 out of 38), Thakurgaon 48.15% (26 out of 54), Satkhira 56.92% (37out of 65), Jhenaidah 33.33% (28 out of 84) and Chattogram 30.79 % (24 out of 78). It is reflected that the selected areas are highly suspected PPR and need to proper vaccination against PPR vaccine that can protect PPR disease in goat and sheep which helps to meet global PPR control strategy as well as contribute to achieve the two (2) number of Sustainable Development Goals (SDGs).&#x0D; Asian Australas. J. Biosci. Biotechnol. 2019, 4 (2), 109-115

Sero-surveillance of circulating PPR virus and its molecular analysis in selected areas of Bangladesh

Peste des Petits Ruminants (PPR) is a highly fatal viral disease of goat and sheep. This research work was done in 2017-2018 by executing, surveillance and epidemiological studies to determine present status of circulating PPR virus and its molecular characterization in different areas of Bangladesh. cELISA was conducted to detect the PPR antibody and RT-PCR also used for identification of N gene PPRV. Sera samples and nasal swabs were collected from eight (8) selected villages under Meherpur sadar upazila of Meherpur district on questionnaire basis. Considering two villages as control and six villages as treatment villages. The total 1860 sera were collected at pre vaccination, 21 days, 3 months, 6 months of post vaccination at these selected areas that tested by cELISA and 8035 goat and sheep were received locally produced PPR Vaccine. Baseline study showed that a total of 950 household rear goats in selected 8 villages where number of goats per household ranges from 4.0-5.0. Deworming was done before vaccination in the treatment villages. Pre-vaccination status of six (6) treatment villages were 55.95%, 50.76%, 37.68%, 41.12%, 44.62% and 43.26% in Chakshamnagar, kola, Amjupi, Amdah, Gopalpur and Chadbill respectively, whereas in the control villages (2) seropositive were 40.00% and 42.57% in Doforpur and Mayamari, respectively. Overall 44.90% goats were seropositive against PPR Virus in treatment villages before vaccination. The Sera was analyzed from 21 days, 3 months and 6 months of post-vaccinated goat and sheep from the treatment (6) villages showed the average herd immunity level of goats and sheep rose to 89.10%, 93.25% and 93.37% respectively whereas in the control villages seropositive goats was 38.14%, 43.98% and 35.64% respectively. Awareness building campaigns with villagers have been conducted involving both men and women through the training, meeting, regular visit of household, distribution of poster and leaflet. The mortality and case fatality rate recorded were 7.4% and 18.8%, respectively due to PPR outbreaks. In clinical case, total 59 nasal swabs were molecular characterized by RT-PCR and 41 (69.49%) samples were N gene positive. Among them, the highest presence of PPR virus was recorded at Meherpur sadar upazila 80.77% (21 out of 26) samples was positive. The result of RT-PCR indicates the PPR virus circulating in the different regions of Bangladesh. It is reflected that locally produced PPR vaccine confers sufficient herd immunity that can protect PPR disease in goat and sheep which helps to meet global PPR control programme.&#x0D; Asian Australas. J. Biosci. Biotechnol. 2019, 4 (2), 88-96

Socioeconomic and Environmental Conditions of Agricultural Migrants in Southwest Region of Bangladesh

This study aimed to identify the socioeconomic and environmental challenges of the migrants who are migrated in Krishnonagar and Mohammadnagar areas of Khulna district. By using simple random sampling technique 348 (157 from Krishnanagar and 191 from Mohammadnagar) household heads were selected as the respondents. The length of living was considered to assess the effect of migration on the socioeconomic and environmental conditions of the respondents. Seven indicators (income, occupation, education, class, family status, poverty situation and health) were considered to assess the socioeconomic conditions of the respondents. The regression analysis supports that the length of migration has its effect on income (71%), poverty situation (66%) and class position (64%) of the respondents. That means the socioeconomic conditions of the people living in the study areas for long time is better than those of new migrants. In this study environmental issues include water and air quality, housing conditions, waste disposal systems and related hazards affected by the respondents as the migrants. The respondents were migrated from different areas of Bangladesh mainly due to natural disaster and crop cultivation related vulnerability. Among the total respondents 87.4 percent of the household heads are male and 12.6 percent are female. The present study shows that nearly half of the respondents (47.7%) in the study area were found to be involved in seasonal work. The average monthly household income in the study areas was 5892 BDT and 55.2 percent respondents were absolutely poor. About 17 percent respondents claimed for the ownership of the house but 57.87 percent were living in rented house. Among the environmental challenges waterlogging and problem of solid waste disposal were severe. In this regard some useful points of recommendations for effective urban management and rural development activities are suggested.&#x0D; South Asian J. Agric., 7(1&amp;2): 6-14

Microbial Contamination and Detection of Antibacterial Activity of Syzygium aromaticum against Food Borne Pathogens

This study was undertaken to find out the presence of contaminating microorganisms in commonly available Syzygium aromaticum samples collected from different areas of Bangladesh and also to evaluate the antibacterial traits of these Syzygium aromaticum samples against food born pathogens. Total viable bacterial count (TVBC) was determined on nutrient agar and for the isolation of specific microorganisms different selective media were used. Crude, ethanol, methanol, hot water and cold water extracts of the samples were prepared for analysing their antibacterial activity using the agar well diffusion method. Furthermore, the minimum inhibitory concentration (MIC) of the crude extracts was determined. TVBC was found between 104 to 106 cfu/g. None of these samples showed the presence of fungus. Staphylococcus spp. was present almost in all the samples between 104 to 106 cfu/g while Bacillus spp. was noticed only in one sample. In vitro antibacterial activity of the crude, methanolic and ethanolic extracts of the samples was found to be effective mostly against Escherichia coli, Klebsiella spp., Listeria spp., Pseudomonas spp. and Bacillus spp. On the contrary, hot water extracts of only two samples showed antibacterial property against Pseudomonas spp., Listeria spp. and Klebsiella spp. MIC was confirmed by using 96 well plate methods and the minimum inhibitory concentration was between 11.75 to 94 mg/ml.&#x0D; Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 1-5

In-Vitro Antibacterial Properties of Ethanol and Methanol Extracts of Betel Leaves Collected from Different Areas of Bangladesh

Current investigation depicted in-vitro antibacterial activity of available betel leaf (sweet, hot and fresh betel leaves) collected from different place of Bangladesh. The in-vitro antimicrobial activities of the ethanol and methanol extracts of the betel leaf were noticeable against tested bacteria. Among 3 categories of samples, the antibacterial action of ethanol extracts of hot betal leaf was found the highest against most of the bacteria except Klebsiella sp. and Staphylococcus sp., while the methanol extract showed antibacterial activity against all the pathogenic strains tested. The highest MIC (1mg/ml) and the lowest MIC values (25mg/ml) of hot betel leaf and sweet betel leaf were found against E. coli and all test organisms, respectively. This evidence revealed that betel leaf extracts can be used to combat against human pathogens.&#x0D; Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 125-127

Status of anthelmintic resistance of gastrointestinal nematodes in organized sheep and goat farms

Anthelmintic resistance has become a global problem that threatens livestock production worldwide. The present study was investigated the status of anthelmintic resistance in gastrointestinal (GI) nematodes of small ruminants in two organized sheep and goat farms in two different areas of Bangladesh by fecal egg count reduction test (FECRT) for albendazole, levamisole and ivermectin. In each farms, naturally infected animals were divided into four groups of 10 animals. Fecal samples from each group were collected on day 0 and day 14 of post treatment to measure the eggs per gram of feces (EPG). The fecal samples of each group before and after treatment were also considered for culture to identify resistant parasite. In case of organized sheep farm, the result of FECRT of albendazole was 90.17, 95% confidence with upper and lower limit was 97.82 and 55.68, respectively. The result of FECRT of levamisole and ivermectin was 98.25 and 96.77, 95% confidence with upper and lower limit was 99.79, 85.12 and 99.11, 88.31, respectively. In case of organized goat farm, the result of FECRT of albendazole was 100, 95% confidence with upper and lower limit was 0 and 0, respectively. The result of FECRT of levamisole and ivermectin was 97.99 and 100, 95% confidence with upper and lower limit was 99.59, 90.28 and 0, 0 respectively. The results revealed that gastrointestinal nematodes were found to be resistant to albendazole in organized sheep farm and suspected to be resistant to levamisole and ivermectin. In organized goat farms, the GI nematodes found to be susceptible to all anthelmintics used for this study. Coproculture revealed that Haemonchus spp. were resistant parasite in sheep farm. This seems to be the first documentation of anthelmintic resistance against GI nematodes in organized sheep and goat farms in Bangladesh. Appropriate measures need to be taken to overcome the situation.&#x0D; Asian J. Med. Biol. Res. December 2018, 4(4): 378-382

New species of the genus Eriovixia Archer, 1951 (Araneae: Araneidae) from Bangladesh

A study on the orb-weaving spider genus Eriovixia Archer was carried out from different areas of Bangladesh. Two species of the genus were recorded of which one species E. nocturnalis nov. sp. is reported to be new to science and another one E. laglaizei (Simon) is a new record for the area of present study. The paper contains generic diagnosis, illustrated description, distribution and key to the species.Bangladesh J. Zool. 46(1): 1-10, 2018

SEROPREVALENCE OF EQUINE BRUCELLOSIS: FIRST REPORT IN BANGLADESH

Despite the endemicity of brucellosis, there is no report on the equine brucellosis in Bangladesh. The Rose Bengal Test (RBT) was used to determine the seroprevalence of Brucella antibodies amongst 112 horses from different areas of Bangladesh. The overall seroprevalence of equine brucellosis was 1.79%.The prevalence recorded in Ghatail area was 3.45% and there was no positive reactor in Shakipur and Savar areas. Sex wise prevalence showed that the prevalence was 3.08%in female and 0.00% in male horse. Only the adult (&gt;3 years of old) horses showed the positive RBT reaction (2.35%), whereas young (&lt;3 years of old) horses did not showed positive RBT reaction. The present study reports the first serological prevalence of Brucella infection in horses in Bangladesh. There is need for the inclusion of horses in brucellosis surveillance and control strategies in Bangladesh to safeguard people from high risk.

An Analysis of Cytogenetic and Clinical Phenotype of Klinefelter Syndrome Over 17 Years

Background: Clinical phenotype in Klinefelter syndrome (KS) shows utmost contrariety according to the genetic presentation. The karyotype 47, XXY is one of the commonest types of sex chromosomal abnormality in males presenting with infertility, hypogonadism, small penis, gynaecomastia and tall stature. Cytogenetic study is the only way to differentiate between chromosomal abnormality and other androgen deficiency disorders. The aim of this study was to investigate cytogenetic and phenotypic profile of Klinefelter syndrome in a group of referred patients with suspected genetic disorders.Methods: This observational study was carried out at the Cytogenetic Laboratory of the Department of Immunology BIRDEM General Hospital for a period of seventeen years from 2000 to 2016. A total of 9,216 patients suspected for different chromosomal abnormalities (e.g. numerical chromosomal disorders, primary amenorrhoea, ambiguous genitalia etc.) were included in this study referred by physicians of various discipline from different areas of Bangladesh. From the patients referred for cytogenetic study, detailed family history and physical findings were noted. Complete genetic examination and pedigree construction was done to exclude non-chromosomal causes of anomalies. For cytogenetic analysis, peripheral lymphocyte culture by the standard method using the G-banding technique was employed.Results: In this study 1.67% (154) of referred patients were diagnosed as Klinefelter syndrome in cytogenetic study and most of them were diagnosed in their adulthood between 20-29 years of age. Classical cytogenetic form of KS-47, XXY (87%) were most common followed by other mosaic and supernumerary X chromosome aneuploidy. Most of the patients presented with tall stature (61.7%) followed by other features such as gynaecomastia (45.5%), eunuchoid skeleton (29.8%), sexual dysfunction (34.41%), small penis (22.7%), and delayed development of secondary sex characteristics (22.7%).Conclusion: Diagnosis of Klinefelter syndrome in early age before puberty is needed to be differentiated from other related disorders and thereby improving the quality of life by providing appropriate and timely treatment. Therefore, we have to focus to improve our overall capacity to diagnose genetic disorders for proper interventionBirdem Med J 2018; 8(2): 126-131

Determining the Hazardous Medical Waste Generation Rates of Private Health Care Facilities-Case Study from Dhaka City of Bangladesh

Many developing countries are lagging behind the developed countries to properly manage their hazardous healthcare waste. These waste pose a significant epidemiological risks to the general people. To estimate the epidemiological risks and to establish parameters of any waste management plan, quantitative estimation of healthcare waste generation is essential. Most researches on quantitative estimation of healthcare wastes to date, have not addressed the differences in waste generation trends between the government and private healthcare sectors. This study was carried out to estimate the hazardous healthcare waste generation from private healthcare sector of Bangladesh; with a future aim do the same for government healthcare sector. A total of 50 privates healthcare facilities of different categories from Dhaka North City Corporation (DNCC) and Dhaka South City Corporation (DSCC) were selected for this study following a random sampling technique. Secondary data was obtained from PRISM Bangladesh (PRISM Bangladesh Foundation is a non-profit voluntary development organization established in 1989. In the context of environmental management, PRISM Bangladesh operates in different areas of Bangladesh with the assistance and support of UN organizations such as UNDP, UNICEF, UNDRO and UNCDF.) for these 50 healthcare facilities and personal visits to waste treatment sites were made. The estimated the hazardous healthcare waste generation rate for the private healthcare sector was found to be 0.17 kg bed-1 day-1 on per patient per day basis and an overall of 6796 kg daily. The estimated waste quantity and the proportion of hazardous healthcare waste to total healthcare waste was found to vary significantly due to different parameters related to the type and size of healthcare facility, seasonal variation etc. Based on the findings of this research, more accurate strategic planning of waste management systems for developing countries can be established by governments and municipal authorities to reduce the epidemiological risks.