Differences In Thermal Sensitivity Research Articles

Metabolic traits predict the effects of warming on phytoplankton competition.

Understanding how changes in temperature affect interspecific competition is critical for predicting changes in ecological communities with global warming. Here, we develop a theoretical model that links interspecific differences in the temperature dependence of resource acquisition and growth to the outcome of pairwise competition in phytoplankton. We parameterised our model with these metabolic traits derived from six species of freshwater phytoplankton and tested its ability to predict the outcome of competition in all pairwise combinations of the species in a factorial experiment, manipulating temperature and nutrient availability. The model correctly predicted the outcome of competition in 72% of the pairwise experiments, with competitive advantage determined by difference in thermal sensitivity of growth rates of the two species. These results demonstrate that metabolic traits play a key role in determining how changes in temperature influence interspecific competition and lay the foundation for mechanistically predicting the effects of warming in complex, multi‐species communities.

Evaluation on intrinsic quality of Wolfiporia cocos influenced by cultivation methods by a multi-step IR macro-fingerprinting technique

Wolfiporia cocos is a well-known traditional East-Asian medicinal fungus. In this study, four Wolfiporia cocos samples (FC-DM, RC-DM, FC-YN and SC-YN) of three cultivation methods harvest from Dabie Mountain area and Yunnan province were discriminated and identified by a multi-step IR macro-fingerprinting technique. The correlation coefficient of the three cultivation methods of Wolfiporia cocos are higher than 0.98 in the range of 450–2600 cm−1. According to retrieving the spectrum in Sadtler database, the main compositions of Wolfiporia cocos are laminarin polysaccharide. While Correlation coefficients of synchronous 2D-IR spectra of FC-DM and other Wolfiporia cocos samples were furtherly calculated, acquiring a quantitative discrimination result. The thermal sensitivity of polysaccharides of SC-YN is different from other samples in the range of 850–1370 cm−1. The difference in thermal sensitivity of CH (1402 and 1474 cm−1) and amino acid (1666 or 1651 cm−1) could also be considered as the key factors to discriminate them. The result proved that the 2DCOS-IR could discriminate Wolfiporia cocos of different cultivation methods.

Altitude influences thermal ecology and thermal sensitivity of locomotor performance in a toad-headed lizard

Population differentiation in ectotherm physiological performance may be driven by adapting to different thermal environments. In this study, we measured locomotor performance in two different altitude populations of the Qinghai toad-headed lizards (Phrynocephalus vlangalii) at different test temperatures to assess between-population differences in thermal sensitivity of sprint speed. Low-elevation lizards ran faster than high-elevation lizards at most test temperatures. Sprint speed varied with test temperature similarly between populations, but the thermal sensitivity (performance breadth) differed significantly. Low-elevation lizards had a lower optimal temperature (Topt) for sprint speed and narrower performance breadth than high-elevation lizards as inferred from the thermal performance curves constructed for each individual. We also measured the body temperature of active lizards (Tb) in the field and selected temperature (Tsel) in the laboratory. Low-elevation lizards had a lower Tsel, and less variable Tb than high-elevation lizards. In both populations, Tsel was lower than Topt for sprint speed, which was inconsistent with the prediction for a match between thermal preference and Topt. Our results suggest that lower thermal sensitivity and weaker locomotor ability for high-elevation lizards may be an adaptive response to the local environmental conditions (e.g., greater thermal variability, higher food availability, and lower predator pressure).

Effect of a patent foramen ovale in humans on thermal responses to passive cooling and heating.

Humans with a patent foramen ovale (PFO) have a higher esophageal temperature (Tesoph) than humans without a PFO (PFO-). Thus the presence of a PFO might also be associated with differences in thermal responsiveness to passive cooling and heating such as shivering and hyperpnea, respectively. The purpose of this study was to determine whether thermal responses to passive cooling and heating are different between PFO- subjects and subjects with a PFO (PFO+). We hypothesized that compared with PFO- subjects PFO+ subjects would cool down more rapidly and heat up slower and that PFO+ subjects who experienced thermal hyperpnea would have a blunted increase in ventilation. Twenty-seven men (13 PFO+) completed two trials separated by >48 h: 1) 60 min of cold water immersion (19.5 ± 0.9°C) and 2) 30 min of hot water immersion (40.5 ± 0.2°C). PFO+ subjects had a higher Tesoph before and during cold water and hot water immersion (P < 0.05). However, the rate of temperature change was similar between groups for each condition. Within a subset of 18 subjects (8 PFO+) who experienced thermal hyperpnea, PFO+ subjects experienced thermal hyperpnea at a higher absolute Tesoph but with a blunted magnitude compared with PFO- subjects. These data suggest that PFO+ subjects have a higher Tesoph at rest and have blunted thermal hyperpnea during passive heating.NEW & NOTEWORTHY Patent foramen ovale (PFO) is found in ~25-40% of the population. The presence of a PFO appears to be associated with a greater core body temperature and blunted ventilatory responses during passive heating. The reason for this blunted ventilatory response to passive heating is unknown but may suggest differences in thermal sensitivity in PFO+ subjects compared with PFO- subjects.

Population level differences in thermal sensitivity of energy assimilation in terrestrial salamanders

Thermal adaptation predicts that thermal sensitivity of physiological traits should be optimized to thermal conditions most frequently experienced. Furthermore, thermodynamic constraints predict that species with higher thermal optima should have higher performance maxima and narrower performance breadths. We tested these predictions by examining the thermal sensitivity of energy assimilation between populations within two species of terrestrial-lungless salamanders, Plethodon albagula and P. montanus. Within P. albagula, we examined populations that were latitudinally separated by >450km. Within P. montanus, we examined populations that were elevationally separated by >900m. Thermal sensitivity of energy assimilation varied substantially between populations of P. albagula separated latitudinally, but did not vary between populations of P. montanus separated elevationally. Specifically, in P. albagula, the lower latitude population had a higher thermal optimum, higher maximal performance, and narrower performance breadth compared to the higher latitude population. Furthermore, across all individuals as thermal optima increased, performance maxima also increased, providing support for the theory that “hotter is better”.

Morphological and physiological determinants of local adaptation to climate in Rocky Mountain butterflies.

Flight is a central determinant of fitness in butterflies and other insects, but it is restricted to a limited range of body temperatures. To achieve these body temperatures, butterflies use a combination of morphological, behavioural and physiological mechanisms. Here, we used common garden (without direct solar radiation) and reciprocal transplant (full solar radiation) experiments in the field to determine the thermal sensitivity of flight initiation for two species of Colias butterflies along an elevation gradient in the southwestern Rocky Mountains. The mean body temperature for flight initiation in the field was lower (24-26°C) than indicated by previous studies (28-30°C) in these species. There were small but significant differences in thermal sensitivity of flight initiation between species; high-elevation Colias meadii initiated flight at a lower mean body temperature than lower-elevation Colias eriphyle. Morphological differences (in wing melanin and thoracic setae) drive body temperature differences between species and contributed strongly to differences in the time and probability of flight and air temperatures at flight initiation. Our results suggest that differences both in thermal sensitivity (15% contribution) and in morphology (85% contribution) contribute to the differences in flight initiation between the two species in the field. Understanding these differences, which influence flight performance and fitness, aids in forecasting responses to climate change.

Adaptive thermal comfort in Australian school classrooms

This survey of thermal comfort in classrooms aimed to define empirically the preferred temperatures, neutral temperatures and acceptable temperature ranges for Australian school children, and to compare them with findings from adult populations. The survey was conducted in a mixture of air-conditioned, evaporative-cooled and naturally ventilated classrooms in nine schools located in three distinct subtropical climate zones during the summer of 2013. A total of 2850 questionnaires were collected from both primary (grade) and secondary (high) schools. An indoor operative temperature of about 22.5°C was found to be the students’ neutral and preferred temperature, which is generally cooler than expected for adults under the same thermal environmental conditions. Despite the lower-than-expected neutrality, the school children demonstrated considerable adaptability to indoor temperature variations, with one thermal sensation unit equating to approximately 4°C operative temperature. Working on the industry-accepted assumption that an acceptable range of indoor operative temperatures corresponds to group mean thermal sensations of −0.85 through to +0.85, the present analysis indicates an acceptable summertime range for Australian students from 19.5 to 26.6°C. The analyses also revealed between-school differences in thermal sensitivity, with students in locations exposed to wider weather variations showing greater thermal adaptability than those in more equable weather districts.

Individual (co)variation in thermal reaction norms of standard and maximal metabolic rates in wild‐caught slimy salamanders

Summary Standard metabolic rate (SMR) and maximal metabolic rate (MMR) are fundamental measures in ecology and evolution because they set the scope within which animals can perform activities that directly affect fitness. In ectotherms, both SMR and MMR are repeatable over time when measured at a single ambient temperature (Ta). Many ectotherms encounter variable Ta from day to day and over their lifetime, yet it is currently unknown whether individual differences hold across an ecologically relevant range of Ta (i.e. thermal repeatability; RT). Moreover, it is possible that thermal sensitivity of SMR and MMR are important individual attributes, and correlated with one another, but virtually nothing is known about this at present. We measured SMR and MMR across an ecologically relevant Ta gradient (i.e. from 10 to 25 °C) in wild‐caught salamanders (Plethodon albagula) and found that RT was significant in both traits. SMR and MMR were also positively correlated, resulting in a lower RT in absolute and factorial aerobic scopes (AAS and FAS). We found significant individual differences in thermal sensitivity for both SMR and MMR, but not for AAS and FAS. The intercept (at Ta = 0 °C) and the slope of the thermal reaction norms were negatively correlated; individuals with low MR at low Ta had a higher thermal sensitivity. Finally, individuals with a high thermal sensitivity for SMR also had high thermal sensitivity for MMR. Our results suggest that natural selection occurring over variable Ta may efficiently target the overall level of – and thermal sensitivity in – SMR and MMR. However, this may not be the case for metabolic scopes, as the positive correlation between SMR and MMR, in addition to their combined changes in response to Ta, yielded little individual variation in AAS and FAS. Our results support the idea that organisms with low metabolism at low Ta have a high metabolic thermal sensitivity as a compensatory mechanism to benefit in periods of warmer environmental conditions. Hence, our study reveals the importance of considering within‐individual variation in metabolism, as it may represent additional sources of adaptive (co)variation.

Individual variation in thermal performance curves: swimming burst speed and jumping endurance in wild-caught tropical clawed frogs

The importance of studying individual variation in locomotor performance has long been recognized as it may determine the ability of an organism to escape from predators, catch prey or disperse. In ectotherms, locomotor performance is highly influenced by ambient temperature (Ta), yet several studies have showed that individual differences are usually retained across a Ta gradient. Less is known, however, about individual differences in thermal sensitivity of performance, despite the fact that it could represent adaptive sources of phenotypic variation and/or additional substrate for selection to act upon. We quantified swimming and jumping performance in 18 wild-caught tropical clawed frogs (Xenopus tropicalis) across a Ta gradient. Maximum swimming velocity and acceleration were not repeatable and individuals did not differ in how their swimming performance varied across Ta. By contrast, time and distance jumped until exhaustion were repeatable across the Ta gradient, indicating that individuals that perform best at a given Ta also perform best at another Ta. Moreover, thermal sensitivity of jumping endurance significantly differed among individuals, with individuals of high performance at low Ta displaying the highest sensitivity to Ta. Individual differences in terrestrial performance increased with decreasing Ta, which is opposite to results obtained in lizards at the inter-specific and among-individual levels. To verify the generality of these patterns, we need more studies on individual variation in thermal reaction norms for locomotor performance in lizards and frogs.

Variation in thermal sensitivity and thermal tolerances in an invasive species across a climatic gradient: lessons from the land snail Cornu aspersum.

The ability of organisms to perform at different temperatures could be described by a continuous nonlinear reaction norm (i.e., thermal performance curve, TPC), in which the phenotypic trait value varies as a function of temperature. Almost any shift in the parameters of this performance curve could highlight the direct effect of temperature on organism fitness, providing a powerful framework for testing thermal adaptation hypotheses. Inter-and intraspecific differences in this performance curve are also reflected in thermal tolerances limits (e.g., critical and lethal limits), influencing the biogeographic patterns of species’ distribution. Within this context, here we investigated the intraspecific variation in thermal sensitivities and thermal tolerances in three populations of the invasive snail Cornu aspersum across a geographical gradient, characterized by different climatic conditions. Thus, we examined population differentiation in the TPCs, thermal-coma recovery times, expression of heat-shock proteins and standard metabolic rate (i.e., energetic costs of physiological differentiation). We tested two competing hypotheses regarding thermal adaptation (the “hotter is better” and the generalist-specialist trade-offs). Our results show that the differences in thermal sensitivity among populations of C. aspersum follow a latitudinal pattern, which is likely the result of a combination of thermodynamic constraints (“hotter is better”) and thermal adaptations to their local environments (generalist-specialist trade-offs). This finding is also consistent with some thermal tolerance indices such as the Heat-Shock Protein Response and the recovery time from chill-coma. However, mixed responses in the evaluated traits suggest that thermal adaptation in this species is not complete, as we were not able to detect any differences in neither energetic costs of physiological differentiation among populations, nor in the heat-coma recovery.

Hot and steady: Elevated temperatures do not enhance muscle disuse atrophy during prolonged aestivation in the ectothermCyclorana alboguttata

Animals that undergo prolonged dormancy experience minimal muscle disuse atrophy (MDA) compared to animals subjected to artificial immobilisation over shorter timeframes. An association between oxidative stress and MDA suggests that metabolic depression presumably affords dormant animals some protection against muscle disuse. Because aerobic metabolism is temperature sensitive, we proposed that MDA in dormant (aestivating) ectotherms would be enhanced at elevated temperatures. In the green-striped burrowing frog, Cyclorana alboguttata, the thermal sensitivity of skeletal muscle metabolic rate is muscle-specific. We proposed that the degree of atrophy experienced during aestivation would correlate with the thermal sensitivity of muscle metabolic rate such that muscles with a relatively high metabolic rate at high temperatures would experience more disuse atrophy. To test this hypothesis, we examined the effect of temperature and aestivation on the extent of MDA in two functionally different muscles: the M. gastrocnemius (jumping muscle) and M. iliofibularis (non-jumping muscle), in C. alboguttata aestivating at 24 or 30 °C for 6 months. We compared a range of morphological parameters from muscle cross-sections stained with succinic dehydrogenase to show that muscle-specific patterns of disuse atrophy were consistent with the relative rates of oxygen consumption of those muscle types. However, despite muscle-specific differences in thermal sensitivity of metabolic rate, aestivation temperature did not influence the extent of atrophy in either muscle. Our results suggest that the muscles of frogs aestivating at high temperatures are defended against additional atrophy ensuring protection of muscle function during long periods of immobilisation.

Regional distribution of thermal sensitivity to cold at rest and during mild exercise in males

Although several studies have compared thermal sensitivity between body segments, little is known on regional variations within body segments. Furthermore, the effects of exercise on the thermal sensation resulting from a cold stimulus remain unclear. The current experiment therefore aimed to explore inter- and intra-segmental differences in thermal sensitivity to cold, at rest and during light exercise. Fourteen male participants (22.3±3.1 years; 181.6±6.2cm; 73.7±10.3kg) were tested at rest and whilst cycling at 30% VO2max. Sixteen body sites (front torso=6; back=6; arms=4) were stimulated in a balanced order, using a 20°C thermal probe (25cm2) applied onto the skin. Thermal sensations resulting from the stimuli were assessed using an 11-point cold sensation scale (0=not cold; 10=extremely cold). Variations were found within body segments, particularly at the abdomen and mid-back where the lateral regions were significantly more sensitive than the medial areas. Furthermore, thermal sensations were significantly colder at rest compared to exercise in 12 of the 16 body sites tested. Neural and hormonal factors were considered as potential mechanisms behind this reduction in thermal sensitivity. Interestingly, the distribution of cold sensations was more homogenous during exercise. The present data provides evidence that thermal sensitivity to cold varies within body segments, and it is significantly reduced in most areas during exercise.

Cortical thickness correlates of pain and temperature sensitivity

It is well established that there is individual variability in pain and temperature sensitivity. Functional brain imaging studies have found that interindividual heat pain variability correlates with brain activity in sensory and pain modulation areas. Thus, it is possible that these individual differences are associated with variability in gray matter thickness of cortical regions involved in thermoreception and pain. To test this, we investigated the relationship between thermal thresholds and cortical thickness in 80 healthy subjects. Subjects underwent a psychophysical session to determine their cool detection (CD), warm detection (WD), cold pain (CP), and heat pain (HP) threshold. A high-resolution structural magnetic resonance imaging scan was acquired for each subject. We correlated each threshold measure to cortical thickness of regions associated with thermoreception and pain. The mean (± SD) thresholds were 30.7°C (± 0.8) for CD, 33.8°C (± 0.7) for WD, 11.7°C (± 9.7) for CP, and 45.3°C (± 2.8) for HP. The brain gray matter analysis revealed a strong correlation between greater thermal and pain sensitivity and cortical thickening of the primary somatosensory cortex. Additionally, greater sensitivity to cool stimuli correlated with cortical thickening in the paracentral lobule, and greater WD correlated with cortical thinning in the anterior midcingulate cortex. We also found that greater HP sensitivity correlated with thickening in the posterior midcingulate cortex and the orbitofrontal cortex. These cortical gray matter correlates of thermal and pain sensitivity provide a neural basis for individual differences in thermal sensitivity.

FDTD analysis of a noninvasive hyperthermia system for brain tumors

BackgroundHyperthermia is considered one of the new therapeutic modalities for cancer treatment and is based on the difference in thermal sensitivity between healthy tissues and tumors. During hyperthermia treatment, the temperature of the tumor is raised to 40–45°C for a definite period resulting in the destruction of cancer cells. This paper investigates design, modeling and simulation of a new non-invasive hyperthermia applicator system capable of effectively heating deep seated as well as superficial brain tumors using inexpensive, simple, and easy to fabricate components without harming surrounding healthy brain tissues.MethodsThe proposed hyperthermia applicator system is composed of an air filled partial half ellipsoidal chamber, a patch antenna, and a head model with an embedded tumor at an arbitrary location. The irradiating antenna is placed at one of the foci of the hyperthermia chamber while the center of the brain tumor is placed at the other focus. The finite difference time domain (FDTD) method is used to compute both the SAR patterns and the temperature distribution in three different head models due to two different patch antennas at a frequency of 915 MHz.ResultsThe obtained results suggest that by using the proposed noninvasive hyperthermia system it is feasible to achieve sufficient and focused energy deposition and temperature rise to therapeutic values in deep seated as well as superficial brain tumors without harming surrounding healthy tissue.ConclusionsThe proposed noninvasive hyperthermia system proved suitable for raising the temperature in tumors embedded in the brain to therapeutic values by carefully selecting the systems components. The operator of the system only needs to place the center of the brain tumor at a pre-specified location and excite the antenna at a single frequency of 915 MHz. Our study may provide a basis for a clinical applicator prototype capable of heating brain tumors.

Effects of heat-shock selection during pelagic stages on thermal sensitivity of juvenile sea cucumber, Apostichopus japonicus Selenka

The effects of heat-shock during pelagic stages on growth and survival of juvenile sea cucumber Apostichopus japonicus were investigated to test the possibility to acquire high thermal tolerance individuals after the heat-shock selection. Larvae at the stages of blastula, gastrula, and auricularia were heat-shocked at selected temperatures (21.5, control; 26, 28, and 30°C) for 45 min and returned to 21.5°C for continuous rearing. There was a significant difference in thermal sensitivity among different developmental stages, and thermal tolerance of the larvae was correlated with the expression of heat shock protein 70 (Hsp70). Juveniles after the heat-shock selection at pelagic stages showed higher induced thermotolerance than those without heat-shock. Therefore, heat-shock application at early pelagic development stages is potentially a useful way to select high-thermotolerance variety of the sea cucumbers.

Putting ourselves in a striped snake's shoes: thermal sensitivity of locomotor performance in a melanistic/striped colour‐dimorphic snake

AbstractIntermorph differences of thermoregulatory abilities in colour‐polymorphic (dimorphic) species have been demonstrated in many ectotherms. Usually, these studies reported slower body warming in pale‐coloured morphs than in dark‐coloured morphs. Under this circumstance, one way in which pale‐coloured individuals can manage their slower body warming is to perform better at lower temperatures than dark‐coloured individuals. If this is the case, the former need not necessarily raise their body temperature to the same level as the latter. Based on this scenario, intermorph differences in thermal sensitivity of performance were examined using the melanistic/striped colour‐dimorphic snake Elaphe quadrivirgata as a model species. As an indication of performance capability, the crawling speed was measured at several temperatures. Although striped individuals exhibited slower body warming than melanistic individuals under experimental conditions, the former did not exhibit faster crawling speed than the latter at lower temperatures. Shape and position of the performance curve were almost identical between melanistic and striped individuals, indicating a highly static nature in thermal sensitivity of crawling. Coupled with the results of field studies, it is suggested that striped individuals manage their slower body warming by efforts of behavioural thermoregulation. The possible significance of ecological performance in the wild was discussed.

ACCOUNTING FOR HUMAN VARIABILITY AND SENSITIVITY IN SETTING STANDARDS FOR ELECTROMAGNETIC FIELDS

Biological sensitivity and variability are key issues for risk assessment and standard setting. Variability encompasses general inter-individual variations in population responses, while sensitivity relates to unusual or extreme responses based on genetic, congenital, medical, or environmental conditions. For risk assessment and standard setting, these factors affect estimates of thresholds for effects and dose-response relationships and inform efforts to protect the more sensitive members of the population, not just the typical or average person. While issues of variability and sensitivity can be addressed by experimental and clinical studies of electromagnetic fields, investigators have paid little attention to these important issues. This paper provides examples that illustrate how default assumptions regarding variability can be incorporated into estimates of 60-Hz magnetic field exposures with no risk of cardiac stimulation and how population thresholds and variability of peripheral nerve stimulation responses at 60-Hz can be estimated from studies of pulsed gradient magnetic fields in magnetic resonance imaging studies. In the setting of standards for radiofrequency exposures, the International Commission for Non-Ionizing Radiation Protection uses inter-individual differences in thermal sensitivity as one of the considerations in the development of "safety factors." However, neither the range of sensitivity nor the sufficiency or excess of the 10-fold and the additional 5-fold safety factors have been assessed quantitatively. Data on the range of responses between median and sensitive individuals regarding heat stress and cognitive function should be evaluated to inform a reassessment of these safety factors and to identify data gaps.

Consequences of Metamorphosis for the Locomotor Performance and Thermal Physiology of the NewtTriturus cristatus

During metamorphosis, most amphibians undergo rapid shifts in their morphology that allow them to move from an aquatic to a more terrestrial existence. Two important challenges associated with this shift in habitat are the necessity to switch from an aquatic to terrestrial mode of locomotion and changes in the thermal environment. In this study, I investigated the consequences of metamorphosis to the burst swimming and running performance of the European newt Triturus cristatus to determine the nature and magnitude of any locomotor trade-offs that occur across life-history stages. In addition, I investigated whether there were any shifts in the thermal dependence of performance between life-history stages of T. cristatus to compensate for changes in their thermal environment during metamorphosis. A trade-off between swimming and running performance was detected across life-history stages, with metamorphosis resulting in a simultaneous decrease in swimming and increase in running performance. Although the terrestrial habitat of postmetamorphic stages of the newt T. cristatus experienced greater daily fluctuations in temperature than the aquatic habitat of the larval stage, no differences in thermal sensitivity of locomotor performance were detected between the larval aquatic and postmetamorphic stages. The absence of variation across life-history stages of T. cristatus may indicate that thermal sensitivity may be a conservative trait across ontogenetic stages in amphibians, but further studies are required to investigate this assertion.

Hemorheology and oxygen transport in vertebrates. A role in thermoregulation?

We studied the effect of temperature on blood rheology in three vertebrate species with different thermoregulation and erythrocyte characteristics. Higher fibrinogen proportion to total plasma protein was found in turtles (20%) than in pigeons (5.6%) and rats (4.2%). Higher plasma viscosity at room temperature than at homeotherm body temperature was observed in rats (1.69 mPa x s at 20 degrees C vs. 1.33 mPa x s at 37 degrees C), pigeons (3.40 mPa x s at 20 degrees C vs. 1.75 mPa x s at 40 degrees C), and turtles (1.74 mPa x s at 20 degrees C vs. 1.32 mPa x s at 37 degrees C). This fact allow us to hypothesize that thermal changes in protein structure may account for an adjustment of the plasma viscosity. Blood viscosity was dependent on shear rate, temperature and hematocrit in the three species. A different behaviour in apparent and relative viscosities between rat and pigeon at environmental temperature was found. Moreover, the blood oxygen transport capacity seems more affected by a reduction of temperature in rats than in pigeons. Both findings indicate a greater influence of temperature on mammalian erythrocyte than on nucleated red cells, possibly as a consequence of differences in thermal sensitivity and mechanical stability between them. A comparison between the three species revealed that apparent blood viscosity measured at homeotherm physiological temperature was linearly related to the hematocrit level of each species. However, when measured at environmental temperature, rat blood showed a higher apparent viscosity than those found in species with non-nucleated red cells, thus indicating a higher impact of temperature decrease on blood viscosity in mammals. This suggest that regional hypothermia caused by cold exposure may affect mammalian blood rheological behaviour in a higher extent than in other vertebrate species having nucleated red cells and, consequently, influencing circulatory function and oxygen transport.

Sensitivity of multidrug-resistant Salmonella typhimurium DT104 to organic acids and thermal inactivation in liquid egg products

A mixture of four Salmonella typhimurium DT104 strains and a mixture of four S. typhimurium non-DT104 strains were examined for their ability to grow in tryptic soy broth (TSB) acidified with acetic, lactic, citric, or malic acids at pH 5·4, 4·4, and 3·7. Significantly (P<0·05) higher numbers of S. typhimurium DT104 cells were detected at pH 4·4 and 4·0 in TSB acidified with acetic acid and at pH 4·4 and 3·7 in TSB acidified with lactic acid compared to non-DT104 cells. Acid-shocked and non-shocked (control) cells were plated on TSA (pH 7·3) acidified with lactic acid at pH 5·4, 4·4, and 4·0 and on TSA (pH 7·0±0·2) containing 0·5, 2·5, and 5% sodium chloride. Populations of acid-shockedS. typhimurium DT104 and non DT104 cells recovered on acidified or salt-supplemented TSA were significantly (P<0·05) lower than those of non-shocked cells. A significantly lower number of acid-shocked non-DT104 cells recovered on TSA at pH 5·4, compared to acid-shocked DT104 cells, suggests that DT104 cells may be more resistant to acid shock and subsequent exposure to acid pH. D values and z values of acid-shocked or non-shocked cells of DT104 and non-DT104 strains in liquid whole egg (WE), egg yolk (EY), egg white (EW), whole egg+10% salt (WES), and egg yolk+10% salt (EYS) were determined. Differences in thermal sensitivity of the two types of cells were few. Rates of thermal inactivation of S. typhimurium DT104 cells indicate that the USDA pasteurization process would eliminate >8 log10cfu ml−1of EW heated at 57°C and >11 log10cfu ml−1of WE, EY, WES, or EYS heated at 61°C. D values of acid-shocked DT104 and non-DT104 cells heated in liquid egg products were significantly (P<0·05) lower than those of respective non-shocked cells.