Abstract Introduction Menopause is known to have a significant impact on mental health, women's quality of life, and sexuality. Objective The purpose of this study is to evaluate the impact of menopause on sexual function in women. The associations of sexual function with demographic and clinical variables were evaluated. Methods We performed a retrospective study comparing the relationship between female sexual function index and menopause in premenopausal women and postmenopausal women. Patients with a diagnosis of benign gynecologic disease who admitted for gynecological surgery at our institution from January 2009 to December 2012 were evaluated. Total 349 women were enrolled in this study and complete the Female Sexual Function Index (FSFI) questionnaires. Risk factors for female sexual function were examined using logistic regression models. Patients were separated into two groups: premenopausal women (group 1), postmenopausal woman (group 2). Women with prior gynecologic surgery were excluded. Continuous variables was compared using the two sample t-test, while non-continuous variables using the Chi-squared test. Results The mean age of the premenopausal women group was 41.7 years, and the average age of the postmenopausal women group was 58.8 years. There was no difference in parity, body weight, height, BMI, disease incidence, and smoking rate between the two groups. Comparing the female sexual function between the two groups, there was a significant relationship between menopause and female sexual function; Desires disorder (p value=0.00), Arousal disorder (p value=0.002), Lubrication disorder (p value=0.003), Orgasm disorder (p value=0.019), Satisfaction disorder (p value=0.002), Pain (p value=0.001), Total FSFI (Premenopausal women; 20.3/postmenopausal women; 15.3, p value=0.001). The result of the FSFI study between the two groups, the postmenopausal women group showed a significant decrease compared to the premenopausal women in all areas. Conclusions Female sexual dysfunction (FSD) is a complex disorder which has an impact on women regardless of their age and race, and related to a variety of biological, psychological, and social determinants. Sexuality impact quality of life through effects on the emotional and psychological health of a woman. Consequently, clinicians who take care of women appreciate when they may be vulnerable to sexual dysfunction. The menopausal transition, a time characterized by hormonal, physiologic, and social changes, is often associated with sexual dysfunction. As postmenopausal woman population is increasing, the further study will be needed in a larger subgroup of women with long-term follow up period. Disclosure No