A series of experiments were conducted to address the need of a psychophysical tool to measure the perceptual segregation of concurrent sources. The experiments measured listeners' sensitivity to the spectral profile of a target sound embedded in a concurrent masker. Both the target and masker were harmonic complexes, which were presented at different fundamental frequencies in order to investigate the effects of this acoustic cue on segregation. The task was designed so that it either strongly encouraged the segregation of the two complexes (task-driven design) or it did not necessarily require segregation (listener-driven design). In both cases, the degree of segregation was assessed by deriving the relative decision weights on the target and masker. Larger differences between the target and masker weights were found as the fundamental frequency difference between the two complexes increased (0.5 – 15 semitones), suggesting that listeners were more successful in selectively attending to the target alone at larger fundamental frequency separations. Although quite different thresholds were obtained for the task-driven and listener-driven designs, the estimates of the decision weights were consistent across the two task designs, indicating that listeners' motivations did not influence the usefulness of the periodicity cue in segregating concurrent sounds.