Abstract Objective: To study responsibility, as one of the stress parameters in workplace, in males and females of open urban population from a gender perspective. Design and method: The study was conducted as a part of cardiological screening in open workable population of both sexes aged 25–64 years in Tyumen. A representative sample was formed from voting lists of 2,000 citizens of one of the administrative districts of Tyumen, 250 people in each of eight groups defined by sex and age (25–34, 35–44, 45–54, 55–64). Response to cardiac screening was 77.7%. To analyze stress at work, questionnaire WHO MONICA-psychosocial “Knowledge and attitude to your health” was applied. In this study, questions regarding workplace responsibility were used to determine stress in the workplace. Results: Statistically significant differences in answers to a question “Has your responsibility at work changed in the past 12 months?” were registered at young age in favor of women (25–34 years: 37.5–54.9%; 35–44: 39.5–53.1% in males and females, respectively, p < 0.01). Over the past 12 months, work responsibility was rated as “insignificant” by 7.9% of males and 3.4% of females in open population, p < 0.001. Similar trends in males and females in relation to this category of answers were detected in age groups of 25–34 (5.7–0.8%, p < 0.05) and 55–64 (18.7–3.7%, p < 0.001). Self-assessment of work responsibility as “high” over the past 12 months was significantly more often detected among females compared to males in general population (53.6–45.2%, p < 0.001) and in elder age group 55–64 (52.8–32.7%, p < 0.001). Conclusions: In open urban population, according to the model of Tyumen city, the highest self-assessment of responsibility in workplace was registered in females, mainly in elder group, and increased work responsibility as in elder age group 55–64 so as predominantly in females younger than 45 years. Thus, generating informational and educational technologies for heart diseases prevention in adult population of the city, top-priority orientation is to be focused on needs of “social risk” groups in population, exactly in young workable females.